scholarly journals Improved assessment of wheat seeds vigor

2012 ◽  
Vol 36 (6) ◽  
pp. 608-614 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco

The tetrazolium test is widely used for seed vigor assessment of various plant species. For wheat, however, there is no methodology specifically recommended. This study aimed at determining an efficient procedure to evaluate vigor of wheat seeds by this test; besides allowing the establishment of seed quality classes. Thus, four different wheat seed lots were assessed by determining the moisture content and through tests of germination, accelerated aging, and field seedling emergence. The following methodologies were assessed: 1) longitudinal bisection of seed, with subsequent placement of the two halves on filter paper moistened with a 1.0% tetrazolium solution, at 30º C, for 2 h; 2) longitudinal bisection, with subsequent immersion of one seed half into a 1.0% tetrazolium solution, at 30º C, for 3 h; and 3) longitudinal bisection, with subsequent immersion of one seed half into a 0.075% tetrazolium solution, at 40º C, for 2 h. It was concluded that the tetrazolium test is more efficient in evaluating wheat seed vigor when performed with immersion of one half of the seed into a 0.075% tetrazolium solution (40º C, for 2 h) or a 0.1% tetrazolium solution (30º C, during 3 h); allowing to sort seeds into four quality classes.

2014 ◽  
Vol 36 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Juliana Tieme Yagushi ◽  
Denis Santiago Costa ◽  
José de Barros França-Neto

Seed deterioration causes losses to seed companies during the storage. For researches, the challenge has been to develop techniques which improve the seed longevity as well as laboratory analysis methods required for evaluating the evolution of deterioration in storage. This study had the objective of determining the effectiveness of the accelerated aging test with a saturated NaCl solution and Seed Vigor Imaging System (SVIS) analysis in checking soybean seed physiological in comparison with the recommended vigor tests. Soybean seeds from the cultivars BRS 184 and M-Soy 7908 RR were used, each of them were represented by six lots and stored for six months under two environment conditions. Seed physiological quality was checked every two months in storage through germination evaluations, accelerated aging (different methods), tetrazolium test (vigor and viability) seedling emergence, and SVIS analysis. The tetrazolium test is the most advisable method for evaluating soybean seed quality during storage. The results of the saturated salt accelerated aging test with NaCl at 41 ºC for 72 or 96 hours and the SVIS evaluation are also both efficient for estimating the physiological potential of seeds.


2014 ◽  
Vol 36 (2) ◽  
pp. 246-252 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Camila Ribeiro de Souza Grzybowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco

The tetrazolium test is part of internal programs of seed quality control because it allows a quick, reliable and accurate assessment of seed viability. The purpose of this study was to determine, among the various methodologies suggested in the literature, a practical and efficient procedure for carrying out the tetrazolium test in sorghum seeds, with a view toward a rapid estimate of their viability. Four seed lots of the simple hybrid Buster were used, testing two forms of seed pre-conditioning (direct immersion in water and between moistened paper towels); two types of preparation (longitudinal cut of the seed through the embryo with immersion of one of the halves in the tetrazolium solution or placement of the two halves on filter paper moistened with tetrazolium solution); two forms of staining (on paper and with direct immersion in the solution) and three concentrations of tetrazolium solution (0.1%, 0.5% and 1.0%). The tetrazolium test may be efficiently conducted for sorghum seeds through pre-conditioning between paper towels for 18 hours at 20 ºC, with immersion staining of one half of the seed in tetrazolium solution at 0.1% for three hours at 40 ºC.


2017 ◽  
Vol 7 (3) ◽  
pp. 146
Author(s):  
Astryani Rosyad ◽  
M. Rahmad Suhartanto ◽  
Abdul Qadir

<p>ABSTRACT<br />Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method<br />between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research<br />was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage.<br />Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulation</p><p>ABSTRAK<br />Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode<br />pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian<br />Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual.<br />Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih,<br />simulasi</p>


2021 ◽  
Vol 43 ◽  
Author(s):  
Carolina Pereira Cardoso ◽  
José Henrique Bizzarri Bazzo ◽  
Jéssica de Lucena Marinho ◽  
Claudemir Zucareli

Abstract: Initial seed vigor and sowing density interact in establishment of plants in the field, and can thus affect expression of the plasticity of the wheat crop and the yield and physiological quality of the seeds produced. The aim of this study was to evaluate the effect of seed vigor levels in combination with sowing densities on the yield and physiological potential of wheat seeds. The cultivars BRS Gralha-Azul and BRS Sabiá were used in a randomized block experimental design in a 2 × 4 factorial arrangement, with four replications. The treatments consisted of seeds of high and low vigor and four sowing densities (150, 250, 350, and 450 seeds.m-2). The following evaluations were made: seed yield, germination, first germination count, seedling length, seedling dry matter, accelerated aging, emergence speed index, and seedling emergence in sand. An increase in sowing density favors the seed yield of both cultivars; however, it reduces the vigor of the seeds produced by the cultivar BRS Gralha-Azul, especially of the seeds produced by plants originating from high vigor seeds. For the cultivar BRS Sabiá, an increase in sowing density decreases the germination performance of seeds produced by plants originating from low vigor seeds.


2003 ◽  
Vol 60 (3) ◽  
pp. 465-469 ◽  
Author(s):  
Angelica Brod Rodo ◽  
Julio Marcos Filho

International research on vegetable seed vigor is not at the same level attained for grain crops species. This study was conducted to identify reliable procedures for the accelerated aging and controlled deterioration tests to rank onion (Allium cepa L.) seed lots according to their physiological potential. Six seed lots of the cultivars Aurora and Petroline were evaluated in the laboratory for germination, first count, seedling vigor classification, traditional and saturated salt accelerated aging (41ºC / 48 and 72 h), controlled deterioration (24% of water / 45ºC / 24 h) and seedling emergence tests. Seed moisture content after the saturated salt accelerated aging test was lower and uniform, which is considered an important advantage in comparison to the traditional procedure. The saturated salt accelerated aging (41ºC / 48 and 72 h) and controlled deterioration (moisture content adjusted to 24% / 45ºC / 24 h) tests were the best procedures to assess the physiological potential of onion seeds, and are indicated for use in quality control programs.


2013 ◽  
Vol 35 (3) ◽  
pp. 361-367 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco

The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability.


2013 ◽  
Vol 35 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Renata Oliveira Alvarenga ◽  
Julio Marcos-Filho ◽  
Tathiana Silva Timóteo

The assessment of physiological potential is essential in seed quality control programs. This study compared the sensitivity of different procedures for evaluating super sweet corn seed vigor, focusing on the primary root protrusion test. Six seed lots, each of the SWB 551 and SWB 585 hybrids, were used. Seed physiological potential was evaluated by germination and vigor tests (speed of germination, traditional and saturated salt accelerated aging, cold test, seedling length, seedling emergence and primary root protrusion). Primary root protrusion was evaluated every 12 hours at 15 °C, 20 °C and 25 °C using two criteria (primary root protrusion and seedlings at the 2 mm root stage). It was concluded that the primary root protrusion test at 15 °C can evaluate super sweet corn seed vigor by counting the number of seedlings at the 2 mm root stage.


2012 ◽  
Vol 34 (4) ◽  
pp. 589-596 ◽  
Author(s):  
Vanessa Neumann Silva ◽  
Francisco Guilhien Gomes Junior ◽  
Silvio Moure Cicero

Nowadays, image analysis is one of the most modern tools in evaluating physiological potential of seeds. This study aimed at verifying the efficiency of the seedling imaging analysis to assess physiological potential of wheat seeds. The seeds of wheat, cultivars IAC 370 and IAC 380, each of which represented by five different lots, were stored during four months under natural environmental conditions of temperature (T) and relative humidity (RH), in municipality of Piracicaba, Stated of São Paulo, Brazil. For this, bimonthly assessments were performed to quantify moisture content and physiological potential of seeds by means of tests of: germination, first count, accelerated aging, electrical conductivity, seedling emergence, and computerized analysis of seedlings, using the Seed Vigor Imaging System (SVIS®). It has been concluded that the computerized analyses of seedling through growth indexes and vigor, using the SVIS®, is efficient to assess physiological potential of wheat seeds.


2013 ◽  
Vol 31 (2) ◽  
pp. 317-321 ◽  
Author(s):  
Salvador B Torres ◽  
Maryjane DA Gomes ◽  
Francisca Gleiciane da Silva ◽  
Clarisse P Benedito ◽  
Francisco ECB Pereira

Germination and vigor tests are essential components of seed quality control programs adopted by seed industries. We studied different procedures to perform the controlled deterioration test to identify differences in vigor among okra seed lots. Four seed lots of Colhe Bem cultivar and five seed lots of Santa Cruz 47 cultivar were submitted to the following tests: germination, seedling emergence, controlled deterioration (seeds with moisture contents of 18, 21 and 24% at 45°C for 24 and 48 hours) and moisture content. The controlled deterioration test is efficient to evaluate the physiological potential of okra seeds, and the combination of 24% water, 45°C during 24 hours is recommended.


Author(s):  
Tauan R. Tavanti ◽  
Renan F. R. Tavanti ◽  
Fernando S. Galindo ◽  
Isadora Simões ◽  
Larissa S. Dameto ◽  
...  

ABSTRACT The use of growth-promoting bacteria can increase the yield and quality of seeds of several annual crops, such as soybean. Based on that, the present study aimed to evaluate the effects of the inoculation of Bacillus subtilis strains on seed treatment, evaluating the yield and quality of seeds of two soybean cultivars. The experiment was conducted in a conventional system, in an Oxisol of clayey texture, in a crop under sprinkler irrigation. The experimental design was randomized blocks, arranged in split plots with four repetitions. Treatments were composed, in the plots, of two soybean cultivars: M7110 IPRO and Brasmax Desafio RR. Within the levels of subplots, the treatments consisted of inoculation and doses of inoculant: Bacillus subtilis Pant001 strain at doses of 0, 2, 3, 4 and 5 mL kg-1, and QST713 strain at the recommended dose of 2 mL kg-1. Inoculation with the strains Pant001 and QST713 increased the yield of both soybean cultivars tested, besides improving seed quality due to the increase in the concentration of total storage proteins, seedling emergence percentage and seed vigor by the accelerated aging test.


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