scholarly journals Pola Penurunan Viabilitas dan Pengembangan Metode Pendugaan Vigor Daya Simpan Benih Pepaya (Carica Papaya L.)

2017 ◽  
Vol 7 (3) ◽  
pp. 146
Author(s):  
Astryani Rosyad ◽  
M. Rahmad Suhartanto ◽  
Abdul Qadir

<p>ABSTRACT<br />Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method<br />between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research<br />was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage.<br />Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulation</p><p>ABSTRAK<br />Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode<br />pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian<br />Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual.<br />Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih,<br />simulasi</p>

2019 ◽  
Vol 2 (3) ◽  
pp. 7
Author(s):  
Dyah Priandini ◽  
Muhamad Rahmad Suhartanto ◽  
Abdul Qadir

Development of papaya fruit production is influenced by the availability of seed quality. High seed quality is maintained during seed storage. Estimation of vigor in relation to storability can be detected by accelerated aging test. This research aims to develop physicall accelerated aging test by, 1)determine the impact of physical accelerated aging on vigor and viability parameters on papaya seed variety Callina and Sukma, 2) determine the levels of seed moisture content and effective period of physicall accelerated aging to predict vigor of papaya seed. This research was conducted at the Laboratory of Seed Science and Technology and Green House Leuwikopo, Department of Agronomy and Horticulture, IPB in January-May 2016 using a randomized complete block design with three replications. Seeds aged by the aging equipment MPC IPB 77-1 MMM. The results showed that the aging time decreased germination value in papaya seed variety Callina and Sukma with equation y=-0.1389x3+3.3333x2–25.25x+81.5 and y=0.0171x3+0.2028x2-9.9956x+81.095. Effective imbibition is 96 hour with moisture content 63-70% in both varieties. The effective of aging time treatment at 0x4, 1x4, 2x4, 3x4, dan 4x4 minutes.Keywords:imbibition, moisture content, viability, vigor


2018 ◽  
Vol 48 (3) ◽  
pp. 28-33 ◽  
Author(s):  
A. Ya. Sotnik

The work presents the evaluation results of the viability of oat and barley collection varieties during storage in the laboratory conditions at the temperature of 20-25o C. Seed storage covered the period of 2006-2017. The research included 24 varieties of barley and 29 varieties of chaffy oats of domestic and foreign breeding collection. The seeds were stored in different packaging materials such as standard paper package; glass bottles of 0.25 liter and plastic bottles of 0.5 liter. The initial seed moisture content was 8.5-9.0 per cent. The varieties were divided into groups taking into account the initial laboratory seed germinating ability. For oats the fi rst group included 10 varieties with initial germination of 96-99%; the second group included 12 varieties with initial germination of 91-95%; the third group included 7 varieties with initial germination of 86-90%. For barley the fi rst group consisted of 5 varieties with initial germination of 91-99%; the second group consisted of 10 varieties with initial germination of 81-90%; the third group consisted of 9 varieties with initial germination of 71-80%. Determination of seed quality was carried out in 7 and 11 years’ time. The initial moisture content of seeds remained without signifi cant changes in all packaging types during the storage period of 11 years. It was also noted that germination of oat and barley seeds during 7 years of storage did not differ in any type of package. The average value in all three groups of oats was 93.2%. After 11 years of storage, germination of oat differed a little, depending on the type of package. In a paper bag it was 80.4%; in a glass bottle – 88.2%; in a plastic bottle – 85.1%. The average initial germination of three groups of barley in 7 years’ time of storage was 84.7%. After 11 years of storage, germination of barley was 53.2% in a paper bag; 63.3% in a glass bottle; 61.8% in a plastic bottle It was revealed that oat and barley seed germination in 7 years’ time did not differ in any type of package. After 11 years, germination of oats was 5.8% higher, and germination of barley was 8.6% higher in a sealed plastic bottle than in a standard paper bag. Differences in seed germinating ability between the glass and plastic bottles were not observed.


2019 ◽  
Vol 41 (4) ◽  
pp. 506-513
Author(s):  
Rafael Vergara ◽  
Raimunda Nonata Oliveira da Silva ◽  
Arieli Paula Nadal ◽  
Gizele Ingrid Gadotti ◽  
Tiago Zanatta Aumonde ◽  
...  

Abstract: Soybean is one of the leading commodities in Brazilian agribusiness. Its cultivation is widespread in different seed-producing regions of the country, where it generates income and local development. In this context, the soybean seed is a fundamental input, as its quality strongly influences the success of the crop. However, the period after the achievement of physiological maturity is critical for the maintenance of seed quality. For that reason, this study aimed at evaluating the effect of harvest delay on both the initial and final qualities of soybean seeds. The research consisted of four harvest times, in which the initial quality was assessed through tests of first germination count, germination, accelerated aging and tetrazolium. After 120 days of storage, the germination and accelerated aging tests were once again conducted. The experiment complied with a completely randomized block design with eight replications. The rainfall was monitored during the pre-harvest phase. All variables experienced negative impacts due to the delay in harvesting. Also, the seeds suffered more damage as the delay progressed, and the variables germination and seed vigor decreased after the storage period.


2012 ◽  
Vol 36 (6) ◽  
pp. 608-614 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco

The tetrazolium test is widely used for seed vigor assessment of various plant species. For wheat, however, there is no methodology specifically recommended. This study aimed at determining an efficient procedure to evaluate vigor of wheat seeds by this test; besides allowing the establishment of seed quality classes. Thus, four different wheat seed lots were assessed by determining the moisture content and through tests of germination, accelerated aging, and field seedling emergence. The following methodologies were assessed: 1) longitudinal bisection of seed, with subsequent placement of the two halves on filter paper moistened with a 1.0% tetrazolium solution, at 30º C, for 2 h; 2) longitudinal bisection, with subsequent immersion of one seed half into a 1.0% tetrazolium solution, at 30º C, for 3 h; and 3) longitudinal bisection, with subsequent immersion of one seed half into a 0.075% tetrazolium solution, at 40º C, for 2 h. It was concluded that the tetrazolium test is more efficient in evaluating wheat seed vigor when performed with immersion of one half of the seed into a 0.075% tetrazolium solution (40º C, for 2 h) or a 0.1% tetrazolium solution (30º C, during 3 h); allowing to sort seeds into four quality classes.


2016 ◽  
Vol 34 (3) ◽  
pp. 219
Author(s):  
Ari Wahyuni ◽  
M. R. Suhartanto ◽  
Abdul Qadir

<p>Soybean seed viability declines during seed storage. Soybean seed deteriorates rapidly, affected by its high protein content and often high humidity in the tropical environment. This research was aimed to develop dynamic model of soybean seed viability in an open storage. The study was conducted in three stages, namely: 1) desk study, 2) seed storage experiment, 3) development of seed storage model, simulation and verification of the model. The second stage of the experiment consisted of soybean seed storing and germination testing using completely randomized design. Treatments were three initial moisture content (7-8%, 9-10% and 11-12%) and four varieties of soybean (Anjasmoro, Wilis, Detam-1 and Detam-2). The results showed that the seed behaviour during storage period were affected by initial seed moisture content, initial viability, varieties and environmental condition. Therefore, seed moisture content, initial viability and varieties may be used as input model. Moisture content, integreting seed respiration, electric conductivity and seed storability vigor (VDSDB) were as model output. Simulation of Seed Storability Vigor Prediction Model with Model Construction Layer-Stella (MCLS) using relative humidity (RH), temperature, seed permeability, initial moisture content and initial viability as input model could logically predict the seed moisture content and seed storability vigor (VDSDB).</p>


2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.


2012 ◽  
Vol 34 (4) ◽  
pp. 541-548 ◽  
Author(s):  
Ísis Barreto Dantas ◽  
João Almir de Oliveira ◽  
Heloisa Oliveira dos Santos ◽  
Édila Vilela Resende Von Pinho ◽  
Sttela Dellyzete Veiga Franco da Rosa

Seed quality may be affected by several factors, including permeability, color, and lignin content in the seed coat. This study aimed at evaluating influence of lignin content in the tegument of seed samples of six different soybean cultivars, in which half of each sample was inoculated with the fungus Aspergillus flavus, on the physical and physiological quality, and on the seed health, during 180 days storage period, under cold chamber with controlled conditions of temperature and RH. For that, at each interval of 60 days, samples were removed, and the physiological quality of these seeds was assessed by means of moisture and lignin contents; and by tests of seed health, germination, and electrical conductivity. The moisture content of seeds remained constant during all storage period. In the seed health test, it was found that inoculation was efficient, once the minimum incidence of the fungus in the inoculated seeds was 85%. In the germination test, there was a trend of reduction on percentage germination with the increase in storage period. However, there was an increase on electrical conductivity of seeds assessed. It was concluded that there is no interference of the lignin content in the seed coat on the resistance to infection by the fungus Aspergillus flavus, even after seed storage for a period of 180 days.


2021 ◽  
Vol 38 (1) ◽  
pp. 144-157
Author(s):  
Thaísa De Oliveira Silveira ◽  
Madelon Rodrigues Braz ◽  
Gilmara Pires de Moura Palermo ◽  
Tiago Böer Breier

Seeds of native forest species for food and seedling production have a growing demand that impacts the need for studies involving seed storage in order to maintain good levels of germination quality. Thus, scientific analyzes that explore the physiological potential of the seeds of these species are necessary, especially for the pink pepper (Schinus terebinthifolia Raddi.), a species used as an alternative source of income for traditional communities and which has been arousing interest due to the consumption of its fruits as a condiment in the national and international market. This work aimed to assess the physiological quality of pink pepper seeds according to the storage period and seed size. The seeds came from a rural settlement, a pioneer in the extraction of pink pepper, in the state of Rio de Janeiro, Brazil. The seeds were evaluated for moisture content, germination, first count, length and dry weight of the germinated seedlings, during the period of time that they remained stored and the size of the seeds. The results allowed to conclude that: i) the germinative percentage of the stored pink pepper seeds decreased over the months; ii) the moisture content of the seeds decreased over the months evaluated iii) the different sizes of seeds did not show differences in germination and storage time and iv) values of length and dry weight of the seedlings did not differ according to the period of storage.


Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3078 ◽  
Author(s):  
Lei Feng ◽  
Susu Zhu ◽  
Chu Zhang ◽  
Yidan Bao ◽  
Xuping Feng ◽  
...  

Seed aging during storage is irreversible, and a rapid, accurate detection method for seed vigor detection during seed aging is of great importance for seed companies and farmers. In this study, an artificial accelerated aging treatment was used to simulate the maize kernel aging process, and hyperspectral imaging at the spectral range of 874–1734 nm was applied as a rapid and accurate technique to identify seed vigor under different accelerated aging time regimes. Hyperspectral images of two varieties of maize processed with eight different aging duration times (0, 12, 24, 36, 48, 72, 96 and 120 h) were acquired. Principal component analysis (PCA) was used to conduct a qualitative analysis on maize kernels under different accelerated aging time conditions. Second-order derivatization was applied to select characteristic wavelengths. Classification models (support vector machine−SVM) based on full spectra and optimal wavelengths were built. The results showed that misclassification in unprocessed maize kernels was rare, while some misclassification occurred in maize kernels after the short aging times of 12 and 24 h. On the whole, classification accuracies of maize kernels after relatively short aging times (0, 12 and 24 h) were higher, ranging from 61% to 100%. Maize kernels with longer aging time (36, 48, 72, 96, 120 h) had lower classification accuracies. According to the results of confusion matrixes of SVM models, the eight categories of each maize variety could be divided into three groups: Group 1 (0 h), Group 2 (12 and 24 h) and Group 3 (36, 48, 72, 96, 120 h). Maize kernels from different categories within one group were more likely to be misclassified with each other, and maize kernels within different groups had fewer misclassified samples. Germination test was conducted to verify the classification models, the results showed that the significant differences of maize kernel vigor revealed by standard germination tests generally matched with the classification accuracies of the SVM models. Hyperspectral imaging analysis for two varieties of maize kernels showed similar results, indicating the possibility of using hyperspectral imaging technique combined with chemometric methods to evaluate seed vigor and seed aging degree.


2003 ◽  
Vol 60 (3) ◽  
pp. 465-469 ◽  
Author(s):  
Angelica Brod Rodo ◽  
Julio Marcos Filho

International research on vegetable seed vigor is not at the same level attained for grain crops species. This study was conducted to identify reliable procedures for the accelerated aging and controlled deterioration tests to rank onion (Allium cepa L.) seed lots according to their physiological potential. Six seed lots of the cultivars Aurora and Petroline were evaluated in the laboratory for germination, first count, seedling vigor classification, traditional and saturated salt accelerated aging (41ºC / 48 and 72 h), controlled deterioration (24% of water / 45ºC / 24 h) and seedling emergence tests. Seed moisture content after the saturated salt accelerated aging test was lower and uniform, which is considered an important advantage in comparison to the traditional procedure. The saturated salt accelerated aging (41ºC / 48 and 72 h) and controlled deterioration (moisture content adjusted to 24% / 45ºC / 24 h) tests were the best procedures to assess the physiological potential of onion seeds, and are indicated for use in quality control programs.


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