scholarly journals Physiological quality and amylase enzyme expression in maize seeds

2013 ◽  
Vol 37 (1) ◽  
pp. 40-48 ◽  
Author(s):  
Gustavo Evangelista Oliveira ◽  
Renzo Garcia Von Pinho ◽  
Thaís de Andrade ◽  
Édila Vilela de Resende Von Pinho ◽  
Custódio Donizete dos Santos ◽  
...  
Keyword(s):  
Selection Process ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Inbred Lines ◽  
Alpha Amylase ◽  
Department Of Agriculture ◽  
Maize Seeds ◽  
Expression Of Genes ◽  
Physiological Quality

The physiological quality of maize seeds is affected by the genotype. Thus, the study of expression of genes associated with this characteristic is important in the genotype selection process in breeding programs. The aim of this research was to study the expression of amylase enzymes associated with physiological quality of maize seeds with different genotypes and seed sizes. We further sought to assess the expression of these enzymes in dry and soaked seeds The experiment was conducted in the experimental area and the Central Seed Laboratory of the Department of Agriculture of the Universidade Federal de Lavras. Seeds of four maize inbred lines were used, classified in two sizes. The physiological quality of the seeds was evaluated by means of germination, seedling emergence, seedling emergence speed index and accelerated aging test. Expression of the alpha amylase enzyme was evaluated by the electrophoresis technique and expression of the alpha amylase B73, alpha amylase (LOC542522) and beta amylase 5 (amyb5) genes was studied by the qRT-PCR technique in dry and soaked seeds of the inbred lines. There is differentiated expression of amylase enzymes in maize seeds of inbred lines with different levels of physiological quality. higher expression of amylase enzymes is observed in soaked maize seeds. The expression of transcripts is higher in smaller as wellas in soaked maize seeds of inbred lines.

scholarly journals Biochemical profiling and its role in physiological quality of maize seeds

2018 ◽  
Vol 40 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Daniele Nerling ◽  
Cileide Maria Medeiros Coelho ◽  
Adriele Brümmer
Keyword(s):  
Soluble Sugars ◽  
Accelerated Aging ◽  
Inorganic Phosphorus ◽  
Inbred Lines ◽  
Total Protein Content ◽  
Breeding Programs ◽  
Maize Hybrids ◽  
Maize Seeds ◽  
Physiological Quality

Abstract: Viability and vigor of seeds are physiological attributes influenced by a myriad of factors including the genotype. Biochemical profiling of maize seeds and its correlation with their physiological quality is of crucial importance in breeding programs that the aim is seeds of better quality. The main goal of this study was to analyze the genetic diversity among maize inbred lines and evaluate the biochemical components that have an important role in physiological quality of seeds. The seeds of the inbred lines and hybrids were submitted to viability test, vigor by the accelerated aging and cold tolerance, and biochemical profiling (total protein content, soluble proteins, total phosphorus, phytate, inorganic phosphorus, starch and soluble sugars). The genetic divergence between the inbred lines found, prompt us to conclude that genetic gains may exist in association with physiological quality of maize hybrids. Seeds with higher inorganic phosphorus and soluble sugars presented superior physiological quality than seeds with lower levels of those compounds.

scholarly journals Effect of seed vigor and sowing densities on the yield and physiological potential of wheat seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Carolina Pereira Cardoso ◽  
José Henrique Bizzarri Bazzo ◽  
Jéssica de Lucena Marinho ◽  
Claudemir Zucareli
Keyword(s):  
Seed Yield ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Crop ◽  
Seedling Length ◽  
Physiological Quality ◽  
Initial Seed ◽  
Wheat Seeds

Abstract: Initial seed vigor and sowing density interact in establishment of plants in the field, and can thus affect expression of the plasticity of the wheat crop and the yield and physiological quality of the seeds produced. The aim of this study was to evaluate the effect of seed vigor levels in combination with sowing densities on the yield and physiological potential of wheat seeds. The cultivars BRS Gralha-Azul and BRS Sabiá were used in a randomized block experimental design in a 2 × 4 factorial arrangement, with four replications. The treatments consisted of seeds of high and low vigor and four sowing densities (150, 250, 350, and 450 seeds.m-2). The following evaluations were made: seed yield, germination, first germination count, seedling length, seedling dry matter, accelerated aging, emergence speed index, and seedling emergence in sand. An increase in sowing density favors the seed yield of both cultivars; however, it reduces the vigor of the seeds produced by the cultivar BRS Gralha-Azul, especially of the seeds produced by plants originating from high vigor seeds. For the cultivar BRS Sabiá, an increase in sowing density decreases the germination performance of seeds produced by plants originating from low vigor seeds.

scholarly journals Physiological quality of dynamically cooled and stored soybean seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 353-360 ◽  
Author(s):  
Jacson Zuchi ◽  
José de Barros França-Neto ◽  
Carlos Sigueyuki Sediyama ◽  
Adílio Flauzino de Lacerda Filho ◽  
Múcio Silva Reis
Keyword(s):  
Dynamic Process ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Superior Performance ◽  
Soybean Seeds ◽  
Tropical Regions ◽  
Physiological Quality ◽  
Feasible Alternative

Storage is an important step in the production of soybean seeds, mainly in tropical regions, where high temperatures can drastically reduce seed quality. The storage at cooler temperatures may be a feasible alternative to preserve seed quality during storage. The objective of this study was to evaluate the physiological quality of soybean seeds artificially cooled by a dynamic process and stored in an environment, with non controlled temperature and relativity humidity. Two seed lots of cultivars Monsoy 8757, TMG 115 RR and BRS Valiosa RR were used. After processing and during packaging the seeds were cooled to 18 ºC by a dynamic process. Seed samples were collected after 0, 60 and 120 days of storage, using the following parameters germination, accelerated aging, seedling emergence in sand, speed of emergence index, electrical conductivity, tetrazolium and seed health. Although soybean seeds artificially cooled by the dynamic method may have slightly superior performance in maintaining physiological quality during storage in non-refrigerated warehouse, for lots of high physiological quality, these advantages are not observed.

scholarly journals Fruit maturation stage on the physiological quality of maroon cucumber seeds

2019 ◽  
Vol 49 ◽  
Author(s):  
Cleisson Dener da Silva ◽  
Andréia Márcia Santos de Souza David ◽  
Josiane Cantuária Figueiredo ◽  
Jorge Luiz Rodrigues Barbosa ◽  
Rayane Aguiar Alves
Keyword(s):  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Dry Mass ◽  
Maturation Stage ◽  
Physiological Maturity ◽  
Physiological Quality ◽  
Photography Analysis ◽  
The Ideal

ABSTRACT To obtain seeds of high physiological quality, it is of paramount importance to define the ideal harvest moment, which oftentimes corresponds to the period when the physiological maturity is reached. This experiment aimed to study the maturation process and determine the best harvest season of maroon cucumber fruits (Liso Gibão cultivar), in order to reach the maximum physiological seed quality. The flowers were labeled during the anthesis and the fruits harvested at 14, 21, 28, 35, 42, 49 and 56 days after the anthesis (DAA). The fruits had their visual aspects registered by photography analysis and, later, the seeds were extracted for physical (water and dry matter contents) and physiological (germination, seedling emergence, emergence speed index, accelerated aging and electrical conductivity) analyses. It was observed that the seeds reach the maximum dry mass (8.08 mg seed-1) between 47 and 49 DAA. However, the seed physiological maturity, characterized by a maximum germination (70 %) and vigor, occurs at 56 DDA. The best season to harvest seeds with a higher physiological quality takes place from 49 to 56 DDA, when the fruits present a yellowish color.

scholarly journals Sowing depth of annual ryegrass seed on different substrates as a vigor test

2019 ◽  
Vol 41 (2) ◽  
pp. 196-204
Author(s):  
Roberto Caetano de Oliveira ◽  
Carlos Eduardo da Silva Pedroso ◽  
Vanessa Nogueira Soares ◽  
Lílian Madruga de Tunes ◽  
Géri Eduardo Meneghello ◽  
...  
Keyword(s):  
Rice Husk ◽  
Soil Cover ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Annual Ryegrass ◽  
Coconut Fiber ◽  
Sowing Depth ◽  
Physiological Quality ◽  
Forage Utilization

Abstract: Annual ryegrass seeds having low physiological quality can lead to the formation of pastures with undesirable plant stands for soil cover and forage utilization pastures. Thus, the objective of this study was proposing a vigor test to classify annual ryegrass seeds. For that, different sowing depths (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 cm) over three substrates (sand, coconut fiber, and carbonized rice husk) were tested to differentiate the physiological quality of four seed lots of annual ryegrass cv. BRS Ponteio having similarities in germination and moisture. The seed lots were classified according to vigor through the field seedling emergence (FSE), 1,000-seeds weight, saturated salt accelerated aging, and germination first count. The 2.5 cm of sand sowing depth and 3.0 cm of coconut fiber sowing depth provided separation of seed lots in similar groups of those reported to FSE test through the seedling emergence and speed of seedling emergence tests.

scholarly journals Quality of maize seeds harvested and husked at high moisture levels

2013 ◽  
Vol 35 (3) ◽  
pp. 276-283 ◽  
Author(s):  
Valquíria de Fátima Ferreira ◽  
João Almir Oliveira ◽  
Thaís Francielle Ferreira ◽  
Leandro Vilela Reis ◽  
Vanessa de Andrade ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seedling Emergence ◽  
Mechanical Damage ◽  
Accelerated Aging ◽  
Cold Test ◽  
High Moisture ◽  
Maize Seeds ◽  
Seed Moisture ◽  
Seed Health Test

Harvest of maize seeds with high moisture levels has been recommended; nevertheless, after harvest, the ears undergo the process of husking and there is a need to assess the compatibility of these processes. The purpose of this study was to assess the effects of mechanical husking on the quality of maize seeds collected at high moisture levels. Dent BM 3061 hybrid maize seeds, manually collected from the ear, with moisture contents of 45%, 40% and 35%, and a 20/64 round sieve were used. The ears were mechanically husked (CWA machine, 312 rpm), dried in a stationary drier at 35 ⁰C until reaching 22% moisture, followed by drying at 42 ºC until reaching 12%. Then, they underwent shelling, followed by a chemical treatment with Maxin® + K-obiol® + Actellic®. Seed quality was assessed according to the occurrence tests of mechanical damage, first count, germination, seedling emergence, emergence speed index, average emergence time, electrical conductivity, accelerated aging, cold test, and seed health test at every quarter for 9 months. The mechanical husking affects maize seed quality, depending on the degree of seed moisture at harvest and on the material studied. This hybrid's seeds can be collected with seed moisture levels at 40% with the use of mechanical husking.

scholarly journals Assessing the physiological quality of common bean seeds using the Vigor-S® system and its relation to the accelerated aging test

2019 ◽  
Vol 41 (2) ◽  
pp. 187-195 ◽  
Author(s):  
André Dantas de Medeiros ◽  
Laércio Junio da Silva ◽  
Nayara Pereira Capobiango ◽  
Camila Andrade Fialho ◽  
Denise Cunha Fernandes dos Santos Dias
Keyword(s):  
Common Bean ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Growth Index ◽  
Automated Analysis ◽  
Seed Vigor ◽  
Analysis Technique ◽  
Physiological Quality

Abstract: Automated analysis of seed vigor stands out by allowing greater accuracy, standardization, objectivity, and speed in evaluation of the physiological potential of seed lots. The objective of this study was to evaluate the efficiency of the Vigor-S® system in assessing the physiological quality of common bean seeds compared to the information provided by the traditional vigor tests recommended for this species. Four genotypes of common bean were used, each one represented by four seed lots. Characterization of the physiological potential of the lots was carried out by the following tests: germination, first count of germination, seedling emergence, accelerated aging, and electrical conductivity. The results of these tests were compared with the data obtained from the image analysis technique, specifically the Vigor-S® system, which was used to evaluate seedling growth at two, three, and four days after the beginning of the germination test. Shoot length, primary root length, and seedling length were measured, as well as the growth index, uniformity index, and vigor index were calculated. Computerized analysis of seedling images using the Vigor-S® software is a reliable alternative for evaluation the physiological potential of bean seeds, and it produces information similar to evaluations traditionally used for that purpose.

scholarly journals Trinexapac-ethyl and topdressing nitrogen levels on the productivity and physiological quality of graniferous white oat seeds

2018 ◽  
Vol 40 (3) ◽  
pp. 263-271
Author(s):  
José Henrique Bizzarri Bazzo ◽  
Carlos Roberto Riede ◽  
Klever Marcio Antunes Arruda ◽  
André Prechlak Barbosa ◽  
Inês Cristina de Batista Fonseca ◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
N Fertilization ◽  
Growth Retardant ◽  
Nitrogen Levels ◽  
Oat Seeds ◽  
Physiological Quality

Abstract: The aim of this study was to evaluate the physiological quality of white oat seeds cultivated under different topdressing N levels combined with the plant growth retardant trinexapac-ethyl in two growing environments. The experiments were carried out in Londrina and Mauá da Serra Municipalities (PR, Brazil) with the cultivar ‘IPR Afrodite’. The experimental design was in randomized blocks, in a 4x2 factorial arrangement, with four replications. Treatments consisted of four N levels (0, 30, 60, and 90 kg.ha-1) combined or not with the plant growth retardant. Seed yield, 1000-seed weight, germination, first germination count, seedling length, seedling dry matter, accelerated aging, electrical conductivity, emergence speed index, and seedling emergence in sand were evaluated. Data were submitted to the analysis of variance and then to the F test and regression analysis up to the second degree (p≤0.05). The application of the plant growth retardant and N topdressing led to an increase in seed productivity in Mauá da Serra. N fertilization reduced seed quality, but this effect can be minimized, not observed or overlapped by the positive effect of the plant growth retardant. Such an effect depends on environmental characteristics and it was not observed in Londrina.

scholarly journals Physiological quality of soybean seeds produced under artificial rain in the pre-harvesting period

2016 ◽  
Vol 38 (1) ◽  
pp. 14-21 ◽  
Author(s):  
Elisa de Melo Castro ◽  
João Almir Oliveira ◽  
Amador Eduardo de Lima ◽  
Heloísa Oliveira dos Santos ◽  
José Igor Lopes Barbosa
Keyword(s):  
Lignin Content ◽  
Block Design ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Moisture Damage ◽  
Soybean Cultivars ◽  
Randomized Block Design ◽  
Physiological Quality ◽  
Storage Times

Abstract: The objective of this experiment was to evaluate responses of soybean cultivars seeds (with different levels of lignin) considering harvest postponement under the incidence of water and the effect of storage. The experiment was conducted in Iraí de Minas, Brazil using a randomized block design with three replications under a 5 x 3 x 3 factorial arrangement, with five soybean cultivars (NK 7059 RR, SYN 1163 RR, SYN 9070 RR, AS 7307 RR and SYN 1283 RR), three harvesting periods (R8, R8 + one rain simulation in the pre-harvesting period and R8 + two simulations in the pre-harvesting period) under three storage times (0, 90 and 180 days). Seeds were evaluated for their chemical composition (lignin contents), the percentage of moisture damage using the tetrazolium test and physiological quality (germination, accelerated aging, conductivity test, seedling emergence and emergence index). Cultivar AS 7307 RR had the highest lignin content in the integument, the lowest percentage of damage by moisture and the highest physiological quality. Cultivars NK 7059 RR and SYN 1163 RR had the lowest lignin contents in the integument and the highest moisture damage. Electrical conductivity increased after storing all cultivars.

scholarly journals PHYSIOLOGICAL QUALITY OF MUNG BEAN SEEDS ACCORDING TO THE PLANT DENSITY

2019 ◽  
Vol 6 (4) ◽  
pp. 29-35
Author(s):  
Edvan Costa Silva ◽  
Nei Peixoto ◽  
Natália Arruda ◽  
Natália Cássia de Faria Ferreira ◽  
Luís Augusto Batista de Oliveira
Keyword(s):  
Mung Bean ◽  
Seed Quality ◽  
Plant Density ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Dry Mass ◽  
Row Spacing ◽  
State University ◽  
Physiological Quality

Mung beans (Vigna radiata L.) are of Asian origin and have gained national importance with the production of moyashi. The study aimed to evaluate the seed physiological quality of mung beans according to the row spacing and number of plants per meter. The experiment was conducted at Goiás State University, Ipameri Campus. Tests were performed to determine the water content, germination, and vigor to evaluate the physiological quality of the seeds. The experimental design was completely randomized, with the treatments arranged in a 2 x 6 factorial scheme, having as factors the row spacing (25 and 50 cm) and the number of plants per meter (4, 8, 12, 16, 20, and 24 plants) with four replications. Based on the results of the analysis of variance, germination, first germination count, and emergence speed index were not influenced by row spacing and number of plants per meter. The shoot length of seedlings was significative affected by row spacing and the number of plants per meter. For root length, seedling dry mass and thousand-seed weight there was significant interaction between the studied factors. There was significant effect of row spacing on accelerated aging and seedling emergence. The mung bean plant density affected seed quality. Seeds produced with a row spacing of 50 cm with 24 plants per meter had the best physiological potential.

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