Effects of caffeine and used coffee grounds on biological features of Aedes aegypti (Diptera, Culicidae) and their possible use in alternative control

Oil-resin fractions from Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) were evaluated for larvicidal activity on third larval instars of Aedes aegypti, in searching for alternative control methods for this mosquito. The bioactive fractions were chemically monitored by thin-layer chromatography, ¹H and 13C nuclear magnetic resonance and mass spectrometry. Bioassays were performed using five repetitions, at a temperature of 28 ± 1°C, relative humidity of 80 ± 5% and light and dark cycles of 12h. Mortality was indicated by darkening of the cephalic capsule after 24h of exposure of the larvae to the solutions. The most active fractions were CRM1-4 (sesquiterpenes) and CRM5-7 (labdane diterpenes), which showed LC50 values of 0.2 and 0.8ppm, respectively.

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EFEKTIFITAS CAMPURAN RENDAMAN JERAMI (ORYZA SATIVA L) DAN TEMEFOS SEBAGAI ATRAKTAN TERHADAP LETHAL OVITRAP AEDES AEGYPTI L

Jurnal Ekologi Kesehatan ◽

10.22435/jek.v19i2.3060 ◽

2020 ◽

Vol 19 (2) ◽

pp. 112-118

Author(s):

Muhammad Rasyid Ridha ◽

Budi Hairani ◽

Gusti Meliyanie ◽

Wulan Rasna Giri Sembiring ◽

Abdullah Fadilly ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Hemorrhagic Fever ◽

Control Strategy ◽

Oryza Sativa L ◽

Larval Mortality ◽

Hemorrhagic Fever ◽

Alternative Control ◽

Randomized Design ◽

Completely Randomized Design ◽

Global Health Problem

ABSTRACT Dengue hemorrhagic fever is a global health problem and can be transmitted through vectors, namely Aedes aegypti. One of the controls can be through lethal ovitrap combined with attractant. This study aims to compare the percentage of trapped eggs, the number of eggs hatched, and larval mortality in the lethal ovitrap that were given two additional types of attractants. The insecticides used were temephos, while the attractants used were 20% straw soaking water, and water that Ae. aegypti have used to lay eggs. The type of research is an experiment with a completely randomized design. The sample used was female mosquito of Ae. aegypti that is full of blood from laboratory colonization. The results showed that straw soaking water was more influential attractant than the former Ae. aegypti colonization water in attracting Ae. aegypti mosquitoes to lay eggs. The highest larval mortality was found in a combination of lethal ovitrap with straw soaking water. Statistically there is an influence of the type of attractant on hatchability and the development of Ae. aegypti into adult mosquitoes. The combination of lethal ovitrap and attractant of straw soaking water can be an alternative control strategy for DHF program managers to reduce the density of Ae. aegypti mosquitoes and minimize transmission of dengue hemorrhagic fever in an area. Keywords: Aedes aegypti, attractant, lethal ovitrap, straw soaking water ABSTRAK Demam berdarah dengue merupakan masalah kesehatan global dan dapat ditularkan melalui vektor yaitu Aedes aegypti. Salah satu pengendaliannya dapat melalui lethal ovitrap yang dipadukan dengan atraktan. Penelitian ini bertujuan untuk membandingkan persentase jumlah telur terperangkap, jumlah telur menetas, dan mortalitas larva pada lethal ovitrap yang diberi tambahan dua jenis atraktan. Insektisida yang digunakan adalah temefos, sedangkan atraktan yang digunakan adalah air rendaman jerami dengan konsentrasi 20%, dan air bekas kolonisasi/telur larva Ae. aegypti. Jenis penelitian ini adalah eksperimen dengan rancangan acak lengkap. Sampel yang digunakan adalah nyamuk Ae. aegypti betina yang kenyang darah hasil kolonisasi di laboratorium. Hasil penelitian menunjukkan bahwa air rendaman jerami merupakan atraktan yang lebih berpengaruh dibandingkan air bekas kolonisasi Ae. aegypti dalam menarik nyamuk Ae. aegypti untuk bertelur. Mortalitas larva tertinggi terdapat pada kombinasi lethal ovitrap dengan air rendaman jerami. Secara statistik ada pengaruh jenis atraktan terhadap daya tetas dan perkembangan Ae. aegypti menjadi nyamuk dewasa. Kombinasi lethal ovitrap dengan atraktan air rendaman jerami dapat menjadi strategi pengendalian alternatif bagi pengelola program DBD untuk mengurangi kepadatan nyamuk Ae. aegypti dan meminimalisasi transmisi penyakit demam berdarah dengue di suatu wilayah. Kata kunci: Aedes aegypti, atraktan, lethal ovitrap, rendaman jerami

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Effect of Used Coffee Grounds on Larval Mortality of Aedes aegypti L. (Diptera: Culicidae): Suspension Concentration and Age versus Efficacy

BioAssay ◽

10.14295/ba.v2.0.52 ◽

2009 ◽

Vol 2 (0) ◽

Author(s):

Marluci Monteiro Guirado ◽

Hermione Elly Melara de Campos Bicudo

Keyword(s):

Aedes Aegypti ◽

Larval Mortality ◽

Suspension Concentration ◽

Coffee Grounds

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ASSESSING THE SUITABILITY OF STERILE INSECT TECHNIQUE APPLIED TOAEDES AEGYPTI

Journal of Biological System ◽

10.1142/s0218339008002691 ◽

2008 ◽

Vol 16 (04) ◽

pp. 565-577 ◽

Cited By ~ 19

Author(s):

CLAUDIA PIO FERREIRA ◽

HYUN MO YANG ◽

LOURDES ESTEVA

Keyword(s):

Biological Control ◽

Cellular Automata ◽

Aedes Aegypti ◽

Control Strategy ◽

Sterile Insect Technique ◽

Alternative Control ◽

Heterogeneous Distribution ◽

Insecticide Application ◽

Mosquito Behavior

The efficacy of biological control of Aedes aegypti mosquitoes using Sterile Insect Technique (SIT) is analyzed. This approach has shown to be very efficient on agricultural plagues and has become an alternative control strategy to the usual technique of insecticide application, which promotes resistance against chemical controls and is harmful to other species that live in the same mosquito habitat. By using a discrete cellular automata approach we have shown that in the case of Aedes aegypti, the spatially heterogeneous distribution of oviposition containers and the mosquito behavior, especially with respect to mating, make the application of STI difficult or impracticable.

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Larvicide and repellent activity of Hypenia irregularis (Benth.) Harley in the alternative control of mosquito Aedes aegypti

Journal of Medicinal Plants Research ◽

10.5897/jmpr2019.6777 ◽

2020 ◽

Vol 14 (10) ◽

pp. 535-543

Author(s):

Dias Possel Richard ◽

Pereira de Souza Ferreira Talita ◽

Martins de Oliveira Bessa Douglas ◽

Oliveira Ferreira Marcela ◽

Patrick Nusrala Dias Diego ◽

...

Keyword(s):

Aedes Aegypti ◽

Repellent Activity ◽

Alternative Control

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Prospecting for special metabolites and larvicidal activity of ethanolic extracts from Azadirachta indica A. Juss. (Neem), collected in Tauá-CE against Aedes aegypti mosquito larvae

Research Society and Development ◽

10.33448/rsd-v10i1.11868 ◽

2021 ◽

Vol 10 (1) ◽

pp. e48410111868

Author(s):

Daniel Alves da Silva ◽

Jakson Fernandes Lima ◽

Glória Fernandes Lima ◽

Luiza Michelly Gonçalves Lima ◽

Luciel Narcísio Conceição Viana ◽

...

Keyword(s):

Aedes Aegypti ◽

Azadirachta Indica ◽

Negative Control ◽

Toxicity Tests ◽

Alternative Control ◽

Isolation And Identification ◽

Phytochemical Study ◽

Study Results ◽

Ethanolic Extracts ◽

Ethanol Extracts

In the search for an alternative control against Aedes aegypti, many types of research are developed to discover substitutes for synthetic insecticides, including the use of oils, extracts, or active constituents from plants to find new insecticidal substances. The present work describes the phytochemical study results and evaluation of toxicity against Aedes aegypti larvae of ethanol extracts from Azadirachta indica A. Juss collected in Tauá-CE. Phytochemical tests were performed by different methods involving colorimetric, precipitation reactions, various metabolites such as alkaloids, anthocyanidins, anthocyanins, steroids, and flavonols, flavonones, triterpenoids was evident in the active extracts. For the toxicity tests, different concentrations of ethanolic extracts (250, 500, 1000 and 2000 μg / mL) were prepared with sterile distilled water and dimethylsulfoxide - DMSO (1%), and then 20 mL of each solution was added. And 25 3rd stage larvae in plastic cups. All bioassays were performed in quadruplicate. DMSO in an aqueous solution was used as a negative control. As a result, the tested extracts proved toxic to Aedes aegypti larvae, with an LC50 value higher than the control (10%) and ranging from 12% to 46%. The results obtained show that all extracts have an important pharmacological and toxicological potential. They deserve to be investigated in studies for the isolation and identification of bioactive compounds.

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Efficacy of used coffee grounds as larvicide against Aedes albopictus (Skuse, 1894) and Ae. aegypti Linné, 1762 (Diptera: Culicidae)

Journal of the European Mosquito Control Association ◽

10.52004/jemca2021.0004 ◽

2021 ◽

pp. 1-4

Author(s):

A. Drago ◽

S. Vettore ◽

S. Martini ◽

M. Dutto

Keyword(s):

Aedes Aegypti ◽

Yellow Fever ◽

Aedes Albopictus ◽

Clear Indication ◽

Coffee Grounds ◽

Unit Dose

Aedes albopictus and Aedes aegypti are two synanthropic, anthropophilic container-breeding mosquitoes. These species are very annoying, but are also vectors of dengue, chikungunya, yellow fever, Zika, and Usutu viruses, and other pathogens. Because these mosquitoes breed very close to humans, cheap homemade methods, as alternatives to commercial insecticides, could be important for their control. Coffee being a very common beverage, the grounds extracted from used coffee capsules have been tested for their larvicidal efficacy. The grounds were extracted with either 30 ml or 70 ml of 65-70 °C water. The content of one capsule was used as a unit dose to treat the quantity of water contained in a medium sized flowerpot tray. The test provided a clear indication that at this dosage, the used coffee capsules were completely ineffective at killing the larvae of Aedes species, so this method cannot be suggested to control these mosquitoes.

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