scholarly journals Production of alcohol by simultaneous saccharification and fermentation of low-grade wheat flour

2006 ◽  
Vol 49 (3) ◽  
pp. 481-490 ◽  
Author(s):  
Marcos Antonio das Neves ◽  
Toshinori Kimura ◽  
Naoto Shimizu ◽  
Kiwamu Shiiba
Keyword(s):  
Ethanol Production ◽  
Wheat Flour ◽  
Simultaneous Saccharification And Fermentation ◽  
Alpha Amylase ◽  
Fermentable Sugars ◽  
Low Grade ◽  
Atp Production ◽  
Two Samples ◽  
Ssf Process ◽  
Simultaneous Saccharification

Two samples of low-grade wheat flour, namely low-grade 1 (LG1) and low-grade 2 (LG2), with different carbohydrate and fibrous content, were used as substrates. The samples were liquefied using various concentrations of alpha- or beta-amylase, in order to optimize the production of fermentable sugars; the enzyme alpha-amylase revealed higher performance. After liquefaction, the simultaneous saccharification and fermentation was conducted in a jar fermentor. Amyloglucosidase was used for saccharification, and dry baker's yeast, S. cerevisiae, for fermentation simultaneously. Glucose was consumed promptly in both cases, LG1 and LG2; ethanol production was considerably higher in LG1 (38.6 g/L), compared to LG2 (24.9 g/L). The maximum ATP production was observed early in the SSF process. LG1 revealed higher potential as substrate for ethanol production.

Ethanol Production from Hydrothermal Pretreated Empty Fruit Bunches

2014 ◽  
Vol 917 ◽  
pp. 80-86
Author(s):  
Mohd Saman Siti Aisyah ◽  
Pacharakamol Petchpradab ◽  
Yoshimitsu Uemura ◽  
Suzana Yusup ◽  
Machi Kanna ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Lignocellulosic Biomass ◽  
Simultaneous Saccharification And Fermentation ◽  
Process Time ◽  
Empty Fruit Bunches ◽  
Separate Hydrolysis And Fermentation ◽  
Common Process ◽  
The Common ◽  
Ssf Process ◽  
Simultaneous Saccharification

Separate hydrolysis and fermentation (SHF) is the common process in producing ethanol from lignocellulosic biomass. Nowadays, simultaneous saccharification and fermentation (SSF) process has been seen as potential process for producing ethanol with shortens process time with higher yield of ethanol. Hence, in the current work, the utilization of empty fruit bunches (EFB) in SSF process was studied. In order to improve saccharification reactivity of EFB, hydrothermal pretreatment at 180 and 220 °C was used to pretreat EFB. The findings showed that SSF has the potential in producing ethanol from EFB.

Ethanol Production from Sugarcane Bagasse by Zymomonas mobilis Using Simultaneous Saccharification and Fermentation (SSF) Process

2009 ◽  
Vol 161 (1-8) ◽  
pp. 93-105 ◽  
Author(s):  
Danielle da Silveira dos Santos ◽  
Anna Carolina Camelo ◽  
Kelly Cristina Pedro Rodrigues ◽  
Luís Cláudio Carlos ◽  
Nei Pereira
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Zymomonas Mobilis ◽  
Simultaneous Saccharification And Fermentation ◽  
Ssf Process ◽  
Simultaneous Saccharification

scholarly journals 2G Ethanol Production From Palm Lignocellulosic Biomass

2016 ◽  
Vol 6 (1) ◽  
pp. 773-779
Author(s):  
Leonard Guimarães Carvalho ◽  
Luiz Felipe A. Modesto ◽  
Donato A. Gomes Aranda ◽  
Nei Pereira Jr
Keyword(s):  
Ethanol Production ◽  
Lignocellulosic Biomass ◽  
Palm Oil ◽  
Oil Palm ◽  
Simultaneous Saccharification And Fermentation ◽  
Fermentable Sugars ◽  
Lignocellulosic Material ◽  
The World ◽  
Oil Residue ◽  
Simultaneous Saccharification

Brazil presents the world's largest potential for the production of palm oil due to nearly 75 million hectares of land suitable for palm culture and advantageous soil and climate. The biomass generated in the production of palm oil (palm pressed fiber, PPF) is mainly composed of lignocellulosic material that can be hydrolyzed into fermentable sugars for further conversion to ethanol. This work evaluated alkaline pretreatment of this palm oil residue and subsequent Simultaneous Saccharification and Fermentation (SSF), achieving a conversion of glucose to ethanol higher than 90% and a concentration equivalent to 22.40 g/L of the alcohol.  

scholarly journals Kinetic modeling of simultaneous saccharification and fermentation of corn starch for ethanol production.

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Wojciech Białas ◽  
Adrian Czerniak ◽  
Daria Szymanowska-Powałowska
Keyword(s):  
Central Composite Design ◽  
Ethanol Production ◽  
Corn Starch ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Kinetics Model ◽  
Ssf Process ◽  
Central Composite ◽  
Simultaneous Saccharification ◽  
Granular Starch

Fuel ethanol production, using a simultaneous saccharification and fermentation process (SSF) of native starch from corn flour, has been performed using Saccharomyces cerevisiae and a granular starch hydrolyzing enzyme. The quantitative effects of mash concentration, enzyme dose and pH were investigated with the use of a Box-Wilson central composite design protocol. Proceeding from results obtained in optimal fermentation conditions, a kinetics model relating the utilization rates of starch and glucose as well as the production rates of ethanol and biomass was tested. Moreover, scanning electron microscopy (SEM) was applied to investigate corn starch granule surface after the SFF process. A maximum ethanol concentration of 110.36 g/l was obtained for native corn starch using a mash concentration of 25%, which resulted in ethanol yield of 85.71%. The optimal conditions for the above yield were found with an enzyme dose of 2.05 ml/kg and pH of 5.0. These results indicate that by using a central composite design, it is possible to determine optimal values of the fermentation parameters for maximum ethanol production. The investigated kinetics model can be used to describe SSF process conducted with granular starch hydrolyzing enzymes. The SEM micrographs reveal randomly distributed holes on the surface of granules.

scholarly journals Synergistic Effect of Multiple Saccharifying Enzymes on Alcoholic Fermentation for Chinese Baijiu Production

2020 ◽  
Vol 86 (8) ◽  
Author(s):  
Bowen Wang ◽  
Qun Wu ◽  
Yan Xu ◽  
Baoguo Sun
Keyword(s):  
Synergistic Effect ◽  
Ethanol Production ◽  
Fermentation Process ◽  
Alcoholic Fermentation ◽  
Simultaneous Saccharification And Fermentation ◽  
Alpha Amylase ◽  
Source Tracking ◽  
Chinese Liquor ◽  
Simultaneous Saccharification ◽  
Fermentation Starter

ABSTRACT Chinese Jiuqu (fermentation starter) provides saccharifying enzymes for baijiu (Chinese liquor) fermentation, which undergoes a simultaneous saccharification and fermentation process. However, the key saccharifying enzymes associated with alcoholic fermentation from Jiuqu and their effects on ethanol production remain poorly understood. In this study, we identified 51 carbohydrate hydrolases in baijiu fermentation by metaproteomics analysis. Through source-tracking analysis, approximately 80% of carbohydrate hydrolases in the baijiu fermentation were provided by Jiuqu. Among these enzymes, alpha-amylase (EC 3.2.1.1) and glucoamylase (EC 3.2.1.3), from Aspergillus, Rhizomucor, and Rhizopus, were positively related to starch hydrolysis and ethanol production, indicating that they were the key saccharifying enzymes associated with alcoholic fermentation in the baijiu fermentation. Moreover, a combined mixture of alpha-amylase and glucoamylase (in a ratio of 1:6, wt/wt) enhanced ethanol production in a simulative baijiu fermentation under laboratory conditions. This result revealed a synergistic effect of multiple saccharifying enzymes on ethanol production in baijiu fermentation. Our study provides a potential approach to improve the efficiency of saccharification and alcoholic fermentation by optimizing the profile of saccharifying enzymes for fermentation of baijiu and other beverages. IMPORTANCE Jiuqu starter provides enzymes to the simultaneous saccharification and fermentation process of baijiu (Chinese liquor) production; however, the key saccharifying enzymes associated with alcoholic fermentation from Jiuqu and their effects on ethanol production remain unclear. We confirmed that Jiuqu was the main source of carbohydrate hydrolases for baijiu fermentation and identified two types of saccharifying enzymes from multiple microbes as the key enzymes associated with alcoholic fermentation. Moreover, a proper combination of multiple saccharifying enzymes could enhance ethanol production in baijiu fermentation. This combination provides an approach to optimize the profile of saccharifying enzymes for enhancing ethanol production in baijiu and other food fermentations.

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

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