scholarly journals Simultaneous Saccharification and Fermentation and Factors Influencing Ethanol Production in SSF Process

Author(s):  
Manikandan Kanagasabai ◽  
Karuppaiya Maruthai ◽  
Viruthagiri Thangavelu
2014 ◽  
Vol 917 ◽  
pp. 80-86
Author(s):  
Mohd Saman Siti Aisyah ◽  
Pacharakamol Petchpradab ◽  
Yoshimitsu Uemura ◽  
Suzana Yusup ◽  
Machi Kanna ◽  
...  

Separate hydrolysis and fermentation (SHF) is the common process in producing ethanol from lignocellulosic biomass. Nowadays, simultaneous saccharification and fermentation (SSF) process has been seen as potential process for producing ethanol with shortens process time with higher yield of ethanol. Hence, in the current work, the utilization of empty fruit bunches (EFB) in SSF process was studied. In order to improve saccharification reactivity of EFB, hydrothermal pretreatment at 180 and 220 °C was used to pretreat EFB. The findings showed that SSF has the potential in producing ethanol from EFB.


2009 ◽  
Vol 161 (1-8) ◽  
pp. 93-105 ◽  
Author(s):  
Danielle da Silveira dos Santos ◽  
Anna Carolina Camelo ◽  
Kelly Cristina Pedro Rodrigues ◽  
Luís Cláudio Carlos ◽  
Nei Pereira

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Wojciech Białas ◽  
Adrian Czerniak ◽  
Daria Szymanowska-Powałowska

Fuel ethanol production, using a simultaneous saccharification and fermentation process (SSF) of native starch from corn flour, has been performed using Saccharomyces cerevisiae and a granular starch hydrolyzing enzyme. The quantitative effects of mash concentration, enzyme dose and pH were investigated with the use of a Box-Wilson central composite design protocol. Proceeding from results obtained in optimal fermentation conditions, a kinetics model relating the utilization rates of starch and glucose as well as the production rates of ethanol and biomass was tested. Moreover, scanning electron microscopy (SEM) was applied to investigate corn starch granule surface after the SFF process. A maximum ethanol concentration of 110.36 g/l was obtained for native corn starch using a mash concentration of 25%, which resulted in ethanol yield of 85.71%. The optimal conditions for the above yield were found with an enzyme dose of 2.05 ml/kg and pH of 5.0. These results indicate that by using a central composite design, it is possible to determine optimal values of the fermentation parameters for maximum ethanol production. The investigated kinetics model can be used to describe SSF process conducted with granular starch hydrolyzing enzymes. The SEM micrographs reveal randomly distributed holes on the surface of granules.


2006 ◽  
Vol 49 (3) ◽  
pp. 481-490 ◽  
Author(s):  
Marcos Antonio das Neves ◽  
Toshinori Kimura ◽  
Naoto Shimizu ◽  
Kiwamu Shiiba

Two samples of low-grade wheat flour, namely low-grade 1 (LG1) and low-grade 2 (LG2), with different carbohydrate and fibrous content, were used as substrates. The samples were liquefied using various concentrations of alpha- or beta-amylase, in order to optimize the production of fermentable sugars; the enzyme alpha-amylase revealed higher performance. After liquefaction, the simultaneous saccharification and fermentation was conducted in a jar fermentor. Amyloglucosidase was used for saccharification, and dry baker's yeast, S. cerevisiae, for fermentation simultaneously. Glucose was consumed promptly in both cases, LG1 and LG2; ethanol production was considerably higher in LG1 (38.6 g/L), compared to LG2 (24.9 g/L). The maximum ATP production was observed early in the SSF process. LG1 revealed higher potential as substrate for ethanol production.


2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH,50°C,48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.


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