scholarly journals Analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum

2009 ◽  
Vol 52 (5) ◽  
pp. 1115-1121 ◽  
Author(s):  
Glauciemar Del-Vechio-Vieira ◽  
Orlando Vieira de Sousa ◽  
Mariza Abreu Miranda ◽  
Luci Senna-Valle ◽  
Maria Auxiliadora Coelho Kaplan
Keyword(s):  
Essential Oil ◽  
Formalin Test ◽  
Second Phase ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Medicinal Use ◽  
Leukocyte Mobilization ◽  
Germacrene D ◽  
Anti Inflammatory

The chemical composition, analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum were investigated. The main compounds found in the essential oil were germacrene D, α-humulene and β-cedrene. The oil, with LD50 of 2.50 g/kg, inhibited the acetic acid-induced writhing at the dose of 200 mg/kg. In the formalin test, the oil inhibited the first phase (200 mg/kg) and the second phase (100 mg/kg and 200 mg/kg). In the hot plate test, after 30 and 60 min of treatment the doses of 100 and 200 mg/kg increased the reaction time. The antiedematogenic effect, reduction on the exudate volume and leukocyte mobilization were observed at the doses of 100 and 200 mg/kg. The results indicated that A. fastigiatum possessed the analgesic and anti-inflammatory properties that supported the popular medicinal use of the plant.

Evaluation of the Anti-Inflammatory and Antinociceptive Properties of p-Cymene in Mice

2012 ◽  
Vol 67 (1-2) ◽  
pp. 15-21 ◽  
Author(s):  
Leonardo R. Bonjardim ◽  
Edisleide S. Cunha ◽  
Adriana G. Guimarães ◽  
Michele F. Santana ◽  
Makson G. B. Oliveira ◽  
...  
Keyword(s):  
Nervous System ◽  
Leukocyte Migration ◽  
Autonomous Nervous System ◽  
Second Phase ◽  
Thermal Stimulus ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
The Central Nervous System ◽  
Anti Inflammatory

We attempted to identify the antinociceptive and anti-inflammatory actions of the monoterpene p-cymene. Firstly, behavioural screening was carried out to verify the influence of p-cymene [25, 50, and 100 mg/kg intraperitoneal (i.p.)] on the central nervous system (CNS) activity. The antinociceptive activity of p-cymene was evaluated by the acetic acidinduced writhing response, formalin, and hot-plate test, respectively. The leukocyte migration induced by injection of carrageenan was used to assess the anti-inflammatory activity. p-Cymene showed depressant activity on CNS after 4 h of treatment and also a possible action on the autonomous nervous system (ANS), mainly at the dose of 100 mg/kg (i.p.). It was found that p-cymene (50 and 100 mg/kg, i.p.) significantly (p < 0.05) reduced the writhing responses induced by acetic acid. p-Cymene also decreased the licking time in the first and second phase, respectively, of the formalin test. The results of the hot-plate test showed that all doses of p-cymene increased significantly the latency time of the response to the thermal stimulus in both licking and jumping parameters. In addition, there was a significantly (p < 0.05) decreased leukocyte migration at all doses of p-cymene. The experimental data demonstrate that p-cymene possesses antinociceptive and anti-inflammatory activities

scholarly journals Antinociceptive and Anti-Inflammatory Activities ofTeucrium persicumBoiss. Extract in Mice

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Abdolhossein Miri ◽  
Javad Sharifi-Rad ◽  
Kaveh Tabrizian ◽  
Ali Akbar Nasiri
Keyword(s):  
Chronic Pain ◽  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Analgesic Effect ◽  
Formalin Test ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Cotton Pellet ◽  
Anti Inflammatory

Background.Therapeutic properties ofTeucriumspecies as antioxidant, antibacterial, analgesic, anticancer, diuretic, and tonic compounds have been proved earlier.Materials and Methods. In this study, the antinociceptive and anti-inflammatory effects of the aqueous extract ofTeucrium persicumon chronic pain, sciatic nerve ligation as a model of neuropathic pain, and inflammatory models were investigated by formalin, hot-plate, and cotton pellet-induced granuloma models in mice, respectively.T. persicumaqueous extracts (100, 200, and 400 mg/kg) were orally gavaged for one week. On 8th day, the time spent and the number of lickings were recorded in formalin test. Morphine and Diclofenac were used intraperitoneally as positive controls. In sciatic nerve ligated animals, as a model of neuropathic pain, doses (100, 200, and 400 mg/kg) ofT. persicumextract (TPE) were orally gavaged for 14 consecutive days. The analgesic effect of this extract was examined 14 days after sciatic nerve ligation using the hot-plate test. Controls received saline and Imipramine (40 mg/kg, i.p.) was used a positive control for neuropathic pain model.Results.In the formalin test, a week oral gavage of all TPE doses (100, 200, and 400 mg/kg) caused a significant decrease on the licking response compared to the control negative animals. In the hot-plate test, doses of 200 and 400 mg/kg showed significant analgesic effects in sciatic nerve ligated animals. Oral gavaged of TPE revealed significant analgesic effect on chronic pain in both formalin test and sciatic nerve ligated animals. The TPEs did not have any significant anti-inflammatory effects in cotton pellet-induced granuloma formation in mice.Conclusions.These results suggest that the aqueous extract fromT. persicumBoiss. produced antinociceptive effects. Its exact mechanism of action still remains indistinct.

scholarly journals Antinociceptive and anti-inflammatory effects of Rhodiola Rosea L. extract in rats

Folia Medica ◽  
2013 ◽  
Vol 55 (3-4) ◽  
pp. 70-75 ◽  
Author(s):  
Nina D. Doncheva ◽  
Anita St. Mihaylova ◽  
Damianka P. Getova
Keyword(s):  
Formalin Test ◽  
Heated Surface ◽  
Rhodiola Rosea ◽  
Paw Edema ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Alcohol Water ◽  
Anti Inflammatory ◽  
Rhodiola Rosea Extract

ABSTRACT BACKGROUND: Rhodiola rosea (golden root) is a unique phytoadaptagen with immunomodulatory, antioxidant, anti-inflammatory and antinociceptive activity. AIM: The aim of this study was to evaluate the antinociceptive and anti-inflammatory effects of the alcohol/water extract of Rhodiola rosea roots in rats. MATERIALS AND METHODS: Thirty male Wistar rats were used in the study. They were divided in 3 groups (n = 10), treated respectively with saline (controls), Rhodiola rosea extract 50 mg/kg bw and 100 mg/kg bw orally. The antinociceptive effect was evaluated using the hot-plate test, Randall-Sellito test and the formalin test. The hot-plate test evaluates the reaction time of rats which are dropped on a heated surface. The analgesy-meter test exerts a force increased at constant rate. In the formalin test we measured the total time spent in licking the injected paw during the early (0-10 min) and late phase (20-30 min) of test. To study anti-inflammatory effect the carrageenan-induced paw edema was used. The paw volume was measured plethysmometrically at 2, 3 and 4 hours. RESULTS: In the hot-plate test Rhodiola rosea increased in both doses the latency reaction compared with that in the controls. In analgesy-meter test Rhodiola rosea in a dose of 50 mg/kg showed a significant increase of pressure reaction compared with the controls. In the formalin test Rhodiola rosea in a dose of 100 mg/kg significantly decreased the paw licking time during the first phase. In the plethysmometer test Rhodiola rosea extract significantly reduced carrageenan-induced paw edema when compared with the saline-induced edema. CONCLUSION: The studied extract of Rhodiola rosea exhibited significant analgesic activity in all the pain models used - inhibition of thermal pain, mechanical hyperalgesia and formalin-induced pain behavior. Significant anti-inflammatory activity was observed from Rhodiola rosea extract in carrageenan induced paw edema in rats.

scholarly journals Anti-nociceptive and anti-inflammatory effects of an aqueous extract of blended leaves of Ocimum gratissimum and Psidium guajava

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Akinyinka O. Alabi ◽  
Abayomi M. Ajayi ◽  
Osarume Omorogbe ◽  
Solomon Umukoro
Keyword(s):  
Acetic Acid ◽  
Aqueous Extract ◽  
Psidium Guajava ◽  
Antinociceptive Activity ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Promising Alternative ◽  
Plate Test ◽  
Ocimum Gratissimum ◽  
Anti Inflammatory

Abstract Background To investigate the antinociceptive and anti-inflammatory activities of aqueous extract of a blended mixture of dried leaves of Ocimum gratissimum and Psidium guajava, a traditional analgesic drug polyherbal (TADP) used as a remedy for pain-related conditions. Methods Antinociceptive activity of TADP (100, 200 and 400 mg/kg) was evaluated in the hot plate test and acetic acid-induced nociception in mice while the anti-inflammatory was evaluated in carrageenan-induced paw oedema in rats. Levels of nitrite, myeloperoxidase, glutathione and malondialdehyde were assayed in carrageenan-induced paw tissue. Results TADP (200 and 400 mg/kg) significantly prolong the latency time in the hot-plate test. TADP (100–400 mg/kg) produced a dose-dependent significant inhibition of the acetic-acid induced abdominal constriction. The antinociceptive activity of TADP in the presence of naloxone and atropine was not reversed whereas yohimbine and glibenclamide significantly reversed it. TADP (100, 200 and 400 mg/kg) significantly reduced the swelling in the carrageenan-induced oedema model and also produced a reduction in the nitrite and myeloperoxidase level. TADP (400 mg/kg) significantly reduced malondialdehyde concentration and increase glutathione level in the carrageenan-induced rat paw. TADP significantly decrease the number of cellular infiltrates in the histopathological assessment. Conclusion These results indicate that polyherbal product containing blended leaves of Ocimum gratissimum and Psidium guajava possess antinociceptive and anti-inflammatory properties, hence represents a promising alternative remedy in inflammation-induced pain.

scholarly journals Evaluation of analgesic and anti-inflammatory activities of Warionia saharae essential oil

2021 ◽  
pp. 1-10
Author(s):  
Mimouna Yakoubi ◽  
Nasser Belboukhari ◽  
Khaled Sekkoum ◽  
Mohammed Bouchekara ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Essential Oil ◽  
Traditional Medicine ◽  
Analgesic Activity ◽  
Inflammatory Diseases ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Standard Drug ◽  
Analgesic Effects ◽  
Test Models ◽  
Anti Inflammatory

Warionia saharae Benth & Coss (W.s) (Asteraceae) is a monospecific genus endemic to Algeria and Morocco. Its leaves are used in their traditional medicine, such as gastrointestinal and inflammatory diseases; for instance, rheumatoid arthritis treatment. In this work, our team investigated the anti-inflammatory and analgesic effects of essential oil extracted from the dried upper parts of Warionia saharae based on different standard experimental test models. The analgesic activity was assessed by central and peripheral models, such as “hot plate” and “writhing” tests on Swiss albino mice. The hot plate test used latency measurements to assess acute cutaneous pain sensitivity, as a result; the latency of the hind-paw pain response was by licking and either shaking or jumping, those occurrences were recorded. Writhing test as a chemical method used to induce pain of peripheral origin in mice by injecting acetic acid intraperitoneally (IP). This results in characteristic stretching behavior of the animals (cramps and contortions). The evaluation of the analgesic activity, shows that the essential oil of this plant induces a decrease in the number of abdominal cramps in the contortion test and a maximum inhibition of pain. As for the anti-inflammatory effect, it was studied by the “paw edema” test, a phlogogenic agent (formaldehyde) was used to stimulate inflammation in the paws of mice. Anti-inflammatory properties can be observed by inhibiting this edema compared to the standard drug Diclofenac. In conclusion, Warionia saharae essential oil (75 mg/kg) showed a strong anti-inflammatory and analgesic activities which supports the conventional use of this plant in traditional medicine.

scholarly journals Borneol, a Bicyclic Monoterpene Alcohol, Reduces Nociceptive Behavior and Inflammatory Response in Mice

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Jackson Roberto Guedes da Silva Almeida ◽  
Grasielly Rocha Souza ◽  
Juliane Cabral Silva ◽  
Sarah Raquel Gomes de Lima Saraiva ◽  
Raimundo Gonçalves de Oliveira Júnior ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Motor Coordination ◽  
Nociceptive Behavior ◽  
Hot Plate ◽  
Rotarod Test ◽  
Hot Plate Test ◽  
Plate Test ◽  
Strength Tests ◽  
Anti Inflammatory ◽  
Motor Abnormality

Borneol, a bicyclic monoterpene, has been evaluated for antinociceptive and anti-inflammatory activities. Antinociceptive and anti-inflammatory activities were studied by measuring nociception by acetic acid, formalin, hot plate, and grip strength tests, while inflammation was prompted by carrageenan-induced peritonitis. The rotarod test was used to evaluate motor coordination. Borneol produced a significant (P<0.01) reduction of the nociceptive behavior at the early and late phases of paw licking and reduced the writhing reflex in mice (formalin and writhing tests, resp.). When the hot plate test was conducted, borneol (in higher dose) produced an inhibition (P<0.05) of the nociceptive behavior. Such results were unlikely to be provoked by motor abnormality. Additionally, borneol-treated mice reduced the carrageenan-induced leukocytes migration to the peritoneal cavity. Together, our results suggest that borneol possess significant central and peripheral antinociceptive activity; it has also anti-inflammatory activity. In addition, borneol did not impair motor coordination.

scholarly journals Analgesic effect of intrathecally administered orexin-A in the rat formalin test and in the rat hot plate test

2002 ◽  
Vol 137 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Tatsuo Yamamoto ◽  
Natsuko Nozaki-Taguchi ◽  
Tanemichi Chiba
Keyword(s):  
Analgesic Effect ◽  
Formalin Test ◽  
Hot Plate ◽  
Orexin A ◽  
Hot Plate Test ◽  
Plate Test

scholarly journals Anti-inflammatory, antipyretic, analgesic, and antioxidant activities of Haloxylon salicornicum aqueous fraction

2019 ◽  
Vol 17 (1) ◽  
pp. 1034-1042
Author(s):  
Riaz Ullah ◽  
Mansour S. Alsaid ◽  
Ali S. Alqahtani ◽  
Abdelaaty A. Shahat ◽  
Almoqbil A. Naser ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Water Extract ◽  
Antioxidant Potential ◽  
High Dose ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Aqueous Fraction ◽  
Plate Test ◽  
Anti Inflammatory ◽  
Haloxylon Salicornicum

AbstractThe medicinal plant Haloxylon salicornicum is utilized for therapeutic purposes. We previously reported the antioxidant potential of hexane fraction and methanol extracts of the same species. However, since these solvents could be clinically toxic, the current findings investigated the pharmacological effects of the water fraction. The pain relieving, antioxidant, anti-inflammatory, and antipyretic potential of H. salicornicum water extract (HEW) were studied at two concentrations (250 and 500 mg/kg) in rodents. The carrageenan stimulated rat paw edema assay was exercised to assess anti inflammatory potential in rats; yeast-stimulated hyperthermia was utilized to test antipyretic activity in mice; analgesic properties were assessed based on acetic acid-induced writhing, tail flicking, and hot-plate test; and antioxidant potential was examined with the 2,2-diphenyl-1-picrylhydrazyl assay. We found that 500 mg/kg HEW inhibited edema by 44.03%. Yeast-induced hyperthermia in mice was reduced by 250 and 500 mg/kg HEW after 30, 60, and 120 min with significant level of (P < 0.001) compared to rectal temperature of yeast administered group. The high dose of HEW (500 mg/kg) improved the reaction time of mice in the hot-plate test from 6.66 ± 0.33 to 11.33 ± 0.49 s after 120 min. In the acetic acid-stimulated writhing test, 250 and 500 mg/kg HEW decreased writhing by 32.71% and 51.40%, respectively, after 20 min. HEW also showed antioxidant effects. These results demonstrate that HEW is bioactive and has therapeutic potential for treating a variety of ailments.

scholarly journals The Antinociceptive and Anti-Inflammatory Activities ofAspidosperma tomentosum(Apocynaceae)

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Anansa Bezerra de Aquino ◽  
Luiz Henrique Agra Cavalcante-Silva ◽  
Carolina Barbosa Brito da Matta ◽  
Willians Antônio do Nascimento Epifânio ◽  
Pedro Gregório Vieira Aquino ◽  
...  
Keyword(s):  
Soluble Fraction ◽  
Ethanolic Extract ◽  
Opioid Antagonist ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Plate Test ◽  
Inflammatory Phase ◽  
Soluble Phase ◽  
Anti Inflammatory

We investigated the antinociceptive and anti-inflammatory activities of the crude ethanolic extract (CEE), its fractions, and the flavonoid isorhamnetin fromAspidosperma tomentosumusing models of nociception and inflammation in mice. In the writhing test, the CEE and its fractions (except for soluble phase, CHCl3100% and EtAcO 100%) at 100 mg/kg p.o. induced antinociceptive activity. Isorhamnetin (100 μmol/kg, p.o.) was also active. In the hot plate test, only the treatment with the fractions Hex : CHCl350%, CHCl3100%, and CHCl3 : MeOH 5% (100 mg/kg, p.o.) increased the latency time, reversed by the opioid antagonist naloxone. Fractions that were active in the hot plate test did not show catalepsy condition. It was observed that CEE, all fractions, and isorhamnetin reduced the formalin effects in the neurogenic phase. In the inflammatory phase, only CEE, isorhamnetin, and CHCl3100% and CHCl3 : MeOH 5% fractions were active. CEE and all fractions, except for CHCl3 : MeOH 10% fraction, isorhamnetin, and soluble fraction were able to produce an antioedematogenic activity in the ear capsaicin-induced edema test. In the thioglycolate-induced peritonitis, only EtAcO 100% fraction was not active. The results demonstrate thatA. tomentosumhas antinociceptive and anti-inflammatory activities in animal models.

scholarly journals Antinociceptive and anti-inflammatory effects of a Geissospermum vellosii stem bark fraction

2016 ◽  
Vol 88 (1) ◽  
pp. 237-248 ◽  
Author(s):  
JOSÉLIA A. LIMA ◽  
THIAGO W.R. COSTA ◽  
LEANDRO L. SILVA ◽  
ANA LUÍSA P. MIRANDA ◽  
ANGELO C. PINTO
Keyword(s):  
Formalin Test ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Second Phase ◽  
Murine Models ◽  
Hot Plate Test ◽  
Rat Paw Edema ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Abdominal Constriction

ABSTRACT Geissospermum vellosii (Pao pereira) is a Brazilian tree whose stem barks are rich in indole alkaloids that present intense anticholinesterase activity. The present study evaluated the effects of a stem bark fraction (PPAC fraction) and ethanolic extract (EE) of Pao pereira in classic murine models of inflammation and pain. The EE and PPAC fraction, both at a dose of 30 mg/kg, significantly reduced mice abdominal constriction induced by acetic acid by 34.8% and 47.5%, respectively. In the formalin test, EE (30 mg/kg) and PPAC fraction (30 and 60 mg/kg) inhibited only the second phase, by 82.8%, 84.9% and 100%, respectively. Compared with indomethacin, similar doses of EE or PPAC fraction were approximately twice as effective in causing antinociception. PPAC fraction was not effective in the hot plate test but reduced the inflammatory response at the second (50.6%) and third (57.8%) hours of rat paw edema induced by carrageenan. Antihyperalgesic activity was observed within 30 min with a peak at 2 h (60.1%). These results demonstrate that compounds in PPAC fraction have anti-inflammatory and antinociceptive activity by a mechanism apparently unrelated to the opioid system. Regardless of similar responses to indomethacin, the effects of PPAC fraction are mainly attributed to acetylcholine actions.

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