scholarly journals Inhibitory activity of root canal irrigants against Candida albicans, Enterococcus faecalis and Staphylococcus aureus

2010 ◽  
Vol 24 (4) ◽  
pp. 406-412 ◽  
Author(s):  
Tatiana Kelly da Silva Fidalgo ◽  
Roberta Barcelos ◽  
Maristela Barbosa Portela ◽  
Rosangela Maria de Araújo Soares ◽  
Rogério Gleiser ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Inhibitory Activity ◽  
Root Canal ◽  
Root Canal Irrigants ◽  
And Staphylococcus Aureus

scholarly journals An assessment of Antimicrobial Activity of Three Endodontic Sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by a Direct Contact Test: An In Vitro Study

2020 ◽  
Author(s):  
Trishnika Chakraborty ◽  
Sonali Taneja
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Microbial Count ◽  
Time Intervals ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
And Staphylococcus Aureus

Abstract For successful endodontic treatment, the antimicrobial property of endodontic filling is mandatory. Hence, the aim of this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by direct contact test (DCT).Materials and MethodsThe antimicrobial efficacy of three different sealers, AH Plus, MTA Fillapex and Endo Sequence BC, was tested against Enterococcus faecalis ATCC 29212, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC 25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspension was drawn and spread over culture plates to determine the colony count using a digital colony counter. Readings were taken at 1 hr (fresh specimen), and then at 24 hrs (after setting). The results were tabulated, and statistical analysis was performed using one-way ANOVA and Tukey’s HSD test.ResultsDCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex and Endoseq BC at both time intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum microbial count for Group 1 (AH Plus) for both time intervals (1 hour and 24 hours).ConclusionEndosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both time intervals, followed by MTA Fillapex and AH Plus.

scholarly journals An assessment of Antimicrobial Activity of Three Endodontic Sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by a Direct Contact Test: An In Vitro Study

2019 ◽  
Author(s):  
Trishnika Chakrabort ◽  
Sonali Taneja
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Microbial Count ◽  
Time Intervals ◽  
Ah Plus ◽  
Endodontic Sealers ◽  
And Staphylococcus Aureus

Abstract For a successful endodontic treatment antimicrobial property of endodontic filling is mandatory. Hence the aim of this in vitro study was to assess the antibacterial efficacy of three Endodontic Sealers on Enterococcus faecalis, Candida albicans and Staphylococcus aureus by direct contact test (DCT). Materials and Methods The antimicrobial efficacy of three different sealers (Group 1= AH Plus, 2= MTA Fillapex and 3= Endo Sequence BC) were tested against Enterococcus faecalis ATCC 29212, Candida albicans ATCC 10231 and Staphylococcus aureus ATCC 25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter 10 μL of suspension was drawn and spread over cultural plates in order to determine the colony count using digital colony counter. Readings were taken at 1 hr (fresh specimen), then at 24 hrs (after setting). The results were tabulated and statistical analysis was done using one way ANOVA and Tukey HSD test Results DCT showed a significant decrease in microbial count in AH Plus,MTA Fillapex and Endoseq BC at both the time intervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximum for Group 1(AH Plus) for both the time intervals (1 hour and 24 hours). Conclusion Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both the time intervals, followed by MTA Fillapex and AH Plus.

scholarly journals Comparison of Antimicrobial Effect of Berberine as an Endodontic Irrigant with that of Other Common Root Canal Irrigants on Three Microorganisms Involved in Persistent Endodontic Infections

2018 ◽  
Vol 8 (1) ◽  
pp. 153
Author(s):  
Zakiyeh Donyavi ◽  
Mohammad Reza Arabestani ◽  
Dara Dastan ◽  
Mohammad Esmaeilzadeh ◽  
Nazanin Shahsavand
Keyword(s):  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis ◽  
Root Canal ◽  
Antimicrobial Effect ◽  
Bacterial Counts ◽  
Anterior Teeth ◽  
Root Canal Irrigants ◽  
Significant Difference ◽  
Endodontic Infections

Background and Aim: The present study investigated the antimicrobial effect of Berberine as an endodontic irrigant on the microorganisms involved in persistent endodontic infections. In this experimental in vitro trial, organisms Enterococcus Faecalis, Staphylococcus Aureus, and Staphylococcus Epidermidis were assessed in a multi-species biofilm tooth model. Methods: Seventy-five single-rooted anterior teeth were collected and standardized to a length of 10mm. The teeth were randomly assigned into 5 groups. The teeth were then autoclaved to confirm being sterile. Afterwards, a biofilm consisting of the three selected bacteria was inoculated into the teeth and they were incubated for 21 days. Results: The comparison of the amount of reduction in viable bacterial counts after irrigation by different solutions among the groups was done by Kruskal-wallis test while the changes of viable bacterial counts before and after irrigation with each solution was done by Wilocoxon Signed Ranks test. No significant difference existed among the studied irrigation solutions regarding the mean reduction of total viable bacterial counts, neither did it exist regarding the viable staphylococcus aureus, staphylococcus epidermidis or Enterococcus faecalis counts after irrigation. MIC of berberine against staphylococcus aureus, staphylococcus epidermidis and Enterococcus faecalis species were 0.5ug/ml, 0.03ug/ml and 1ug/ml, respectively. These values were found to be 0.39ug/ml, 0.09ug/ml and 0.78ug/ml for NaOCl and 0.04ug/ml, 0.04ug/ml and 0.09ug/ml for chlorhexidine, respectively. Conclusion: it is concluded that Berberine can be used as a natural alternative instead of conventional root canal irrigants. However, more studies are required in order to confirm that characteristics of this substance are appropriate from other aspects.

scholarly journals Antimicrobial Effect of Conventional Root Canal Medicaments vs Propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans

2012 ◽  
Vol 13 (3) ◽  
pp. 305-309 ◽  
Author(s):  
Sudha Mattigatti ◽  
Deepak Jain ◽  
P Ratnakar ◽  
Shridhar Moturi ◽  
Sarath Varma ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Agar Plate ◽  
Brain Heart Infusion ◽  
Antimicrobial Effect ◽  
P Value ◽  
Agar Diffusion Test ◽  
Post Hoc

ABSTRACT Aim To evaluate and compare antimicrobial effect of various root canal medicaments against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. Materials and methods Six root canal medicaments: 2% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) Calcium hydroxide (Ca(OH)2), EDTA, MTAD and propolis and three microorganisms: Staphylococcus aureus, Enterococcus faecalis and Candida albicans were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37 degrees C for 24 hours. For the agar diffusion test (ADT), petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth injunction. Paper disks were immersed in the experimental solutions for 1 minute. Subsequently, four papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were incubated at 37°C for 48 hours. The diameter of microbial inhibition was measured around the papers disks containing the substances. One way ANOVA followed by Tukey's post-hoc test were used. p-value <0.05 was considered statistically significant. Results Propolis and other irrigants were found to be effective on C. albicans, S. aureus and E. faecalis. CHX and MTAD were found to be most effective amongst all the materials tested followed by propolis. Conclusion Propolis showed antimicrobial activity against E. faecalis, S. aureus, C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. Clinical significance Propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. It could be used as an alternative intracanal medicament. How to cite this article Mattigatti S, Jain D, Ratnakar P, Moturi S, Varma S, Rairam S. Antimicrobial Effect of Conventional Root Canal Medicaments vs Propolis Against Enterococcus faecalis, Staphylococcus aureus and Candida albicans. J Contemp Dent Pract 2012;13(3):305-309.

The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

2019 ◽  
Vol 18 (5) ◽  
pp. 262-274
Author(s):  
E. Benyagoub ◽  
N. Nabbou ◽  
S. Boukhalkhel ◽  
I. Dehini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Fusidic Acid ◽  
Quercus Robur ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

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