scholarly journals Characterization of genetic variability among common bean genotypes by morphological descriptors

2012 ◽  
Vol 12 (1) ◽  
pp. 76-84 ◽  
Author(s):  
Marilene Santos de Lima ◽  
José Eustáquio de Souza Carneiro ◽  
Pedro Crescêncio Souza Carneiro ◽  
Camila Santana Pereira ◽  
Rogério Faria Vieira ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Common Bean ◽  
Genetic Divergence ◽  
Genetic Similarity ◽  
Morphological Descriptors ◽  
Germplasm Bank ◽  
Breeding Programs ◽  
Genetic Dissimilarity

The purpose of this study was to characterize the genetic variability in 100 genotypes of the Active Germplasm Bank of common bean of the Federal University of Viçosa, by morphological descriptors, classify them in groups of genetic similarity and to identify the degree of relevance of descriptors of genetic divergence. The genotypes were evaluated based on 22 quantitative and qualitative morphological descriptors. The high-yielding genotypes V 7936, Gold Gate, LM 95103904, 1829 S 349 Venezuela, and PF 9029975, CNFC 9454 and Fe 732015, with upright growth, have potential for use as parents in common bean breeding programs. By genetic divergence analysis, the genotypes were clustered in eight groups of genetic dissimilarity. By methods of principal components, 9 of the 22 descriptors were eliminated, for being redundant or little variable, suggesting that 10-20 morphological descriptors can be used in studies of characterization of genetic variation.

scholarly journals Evaluating the genetic variability of rubber hybrid progenies of the two crosses PB260 x RO44/71 and PB260 x RO62/54 by RAPD technique

2019 ◽  
Vol 2 (3) ◽  
pp. 36-43
Author(s):  
Thien Minh Nguyen ◽  
Tien Thi My Pham
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Field Experiments ◽  
Agronomic Traits ◽  
Genetic Relationships ◽  
Genetic Material ◽  
Population Based ◽  
Shannon Index ◽  
Polymorphic Bands

The agronomic values of this population have been evaluated in the field experiments based on their phenotypic performance of agronomic traits, but the genetic variability of this population needs to be evaluated via techniques based on genetic material - DNA. In this study, the genetic variability in the investigated population of 71 hybrids and their parents was evaluated by RAPD technique, using eight selected arbitrarily primers; Genetic parameters and dendrogram expressing the genetic relationships among the investigated population were analyzed by GenALEx 6.1, Popgene 1.31 and NTSYSpc 2.1 softwares. Eight primers were used to generate the amplify products on each individual in the investigated population. From 74 genotypes, a total of 109 fragments were generated, among which, there were 89 polymorphic bands representing 81.65% with an average of 11 polymorphic bands/primer. Genetic similarity coefficient among the investigated population, based on DICE coefficient, ranged from 0.560 (LH05/0822 and PB260) to 0.991 (LH05/0781 and LH05/0841) with an average of 0,796, meaning that the genetic distance among ranged from 0.009 to 0.440 with an average of 0.231. The Shannon index and mean heterozygosity values were 0.328 and 0,176, respectively. This indicated that the progenies of the two investigated crosses possessed a relatively high range of genetic variability. The analysis of molecular variance (AMOVA) showed that genetic variation within population represented 62%, while genetic variation among two different crosses contributes 38% to the total genetic variability. Dendrogram based on DICE’s genetic similarity using UPGMA method showed that the hybrids divide into two major genetic groups (0.75), but the crosses were scattered independently of the hybrid.

scholarly journals VARIABILIDADE GENÉTICA UTILIZANDO DESCRITORES MORFOLÓGICOS PARA ACESSOS DE Capsicum spp. PARA FINS ORNAMENTAIS

Nativa ◽  
2019 ◽  
Vol 7 (6) ◽  
pp. 675
Author(s):  
Petterson Baptista da Luz ◽  
Alessandro Aparecido Brito dos Santos ◽  
Valdete Campos Ambrosio
Keyword(s):  
Genetic Variability ◽  
Ornamental Plants ◽  
Growth Potential ◽  
Breeding Programs ◽  
Canonical Correlations ◽  
Plant Parts ◽  
Potted Plants ◽  
Capsicum Spp ◽  
Biometric Templates

O mercado de pimentas para fins ornamentais é um setor recém explorado, mas com grande potencial de crescimento no ramo de floricultura e paisagismo. As pimenteiras que possuem folhagem variegada, porte baixo, frutos com coloração variada em seus diferentes estádios de maturação, contrastando com as folhagens, já são utilizadas como plantas ornamentais, cultivadas e comercializadas em vasos. O presente trabalho teve como objetivo avaliar a variabilidade genética visando o potencial ornamental de acessos de Capsicum spp. através de modelos biométricos. Foram utilizados 55 acessos de Capsicum spp, e a caracterização morfoagronômica dos acessos foi realizada avaliando diferentes partes da planta. O terceiro par de correlações canônicas, com correlação de 0,51, associa plantas com área do dossel compacto-densa, com menor comprimento e largura de dossel e de pequeno porte, associado com folhagens estreitas e de pecíolo grande. Esta associação é vantajosa para ornamentação. Ficou evidenciada a presença de variabilidade genética na população, o que é bastante favorável ao melhoramento na medida em que a heterogeneidade genética possibilita a obtenção de ganhos por meio de seleção.Palavras-chave: pimenta; modelos biométricos; correlações canônicas; análise de trilha. GENETIC VARIABILITY FOCUSED ON ORNAMENTAL POTENTIAL OF A Capsicum spp. ACCESSION ABSTRACT: Pepper market for ornamental purposes is recently exploited; however, it has a great growth potential in floriculture and landscaping. Pepper with variegated foliage, small size, and varied fruit coloration at different ripening stages contrasting with leaves have already been used as ornamental plants cultivated and purchased as potted plants. This study aimed to evaluate genetic variability of Capsicum spp. focusing on its ornamental potential by means of biometric templates. Fifty-five Capsicum spp. accessions were used and a morpho-agronomic characterization of the accessions were performed by evaluating different plant parts. The third canonical correlations are 0.51 and it associates compact-dense, small, shorter length and width canopy plants with narrow foliage and large petiole, which is advantageous for ornamentation. Genetic variation was evidenced in the population being very favorable for plant breeding programs once genetic heterogeneity enables obtaining gains through selections.Keywords: pepper; biometric templates; canonic correlations; trail analysis.

Evaluation and genetic analysis of common bean genotypes (Phaseolus vulgaris L.) at Dibatie and Mandura, Northwestern Ethiopia

2021 ◽  
Vol 7 (5) ◽  
pp. 82-90
Author(s):  
Gezahegn Tefera ◽  
Keyword(s):  
Genetic Variability ◽  
Common Bean ◽  
Coefficient Of Variation ◽  
Environmental Variability ◽  
Genetic Constitution ◽  
Genetic Advance ◽  
Breeding Programs ◽  
Days To Flowering ◽  
Lattice Design ◽  
Component Traits

Assessing and understanding the variations existing in crops due to genetic composition and environmental variability is very important in order to exploit the genetic constitution of crop plants. To this aim, variability measures such as phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) are commonly used. Heritability and genetic advance are major concerns for common bean to identify important traits for common bean genetic improvement. The field experiment was conducted at Mandura and Dibatie research substations working with sixteen genotypes of the common bean during 2017/2018 in lattice design with three replications to evaluate the performance of common bean genotypes and estimate the genetic variability. Heritability and genetic advance were estimated in relation to yield and its component traits for future breeding programs. Combined analysis of variance across locations revealed highly significant variations among genotypes for all traits under study. The PCV ranged from 3.36% for days to flowering to 15.91% for a number of pods per plant while the GCV value ranged from 0.75% for days to flowering to 13.74% for the number of pods per plant. Broad sense heritability values ranged from 5.00% for days to flowering to 84.61% for a hundred seed weight. Generally, the result of the study showed that significant genetic variability among tested genotypes and a simple selection for effective improvement of these traits. Keywords: common bean, genetic variability, genetic advance, heritability

scholarly journals Agronomic potential and selection of okra hybrids to obtain potential genitors

2018 ◽  
Vol 36 (1) ◽  
pp. 112-117 ◽  
Author(s):  
Gabriel M Maciel ◽  
Rafael R Finzi ◽  
Alexandre William C Marra ◽  
Fábio J Carvalho ◽  
Ana Paula O Nogueira
Keyword(s):  
Genetic Divergence ◽  
Canonical Analysis ◽  
Index Number ◽  
Clustering Methods ◽  
Germplasm Bank ◽  
Breeding Programs ◽  
Agronomic Potential ◽  
Per Se ◽  
Fruit Mass ◽  
Selection Of

ABSTRACT Evaluation of pre-commercial hybrids in a germplasm bank is essential for determining its commercial potential or its utility as a potential genitor in a breeding program. The objective of this study was to determine genetic divergence and per se behavior of 47 pre-commercial hybrids from okra germplasm bank of the Universidade Federal de Uberlândia. Precocity index (%), number of fruits (fruits per plant), average fruit mass (g) and productivity (g per plant) were evaluated. Analysis of genetic divergence was performed by multivariate analysis using Mahalanobis distance with different clustering methods (UPGMA and canonical analysis). The performance of hybrids was compared by Scott-Knott (p= 0.05). A significant genetic variability among okra hybrids was observed. UPGMA and canonical analysis grouped the hybrids similarly, being satisfactory to represent genetic divergence. Ten hybrids presented higher performance than the commercial hybrids. Among them, UFU-QB16 stood out as the most promising hybrid for being used as a potential parent in breeding programs after auto pollination.

scholarly journals Genetic Characterization of Cleveland Bay Horse Breed

Diversity ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 174 ◽  
Author(s):  
Khanshour ◽  
Hempsey ◽  
Juras ◽  
Cothran
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Genetic Resource ◽  
Genetic Characterization ◽  
Genetic Relationships ◽  
Similarity Measures ◽  
Horse Breed ◽  
The Usa

The Cleveland Bay (CB) is the United Kingdom’s oldest established horse breed. In this study we analyzed the genetic variability in CB horses and investigated its genetic relationships with other horse breeds. We examined the genetic variability among 90 CB horses sampled in the USA compared to a total of 3447 horses from 59 other breeds. Analysis of the genetic diversity and population structure was carried out using 15 microsatellite loci. We found that genetic diversity in CB horses was less than that for the majority of other tested breeds. The genetic similarity measures showed no direct relationship between the CB and Thoroughbred but suggested the Turkman horses (likely in the lineage of ancestors of the Thoroughbred) as a possible ancestor. Our findings reveal the genetic uniqueness of the CB breed and indicate its need to be preserved as a genetic resource.

scholarly journals Genetic diversity in common bean accessions evaluated by means of morpho-agronomical and RAPD data

2007 ◽  
Vol 64 (3) ◽  
pp. 256-262 ◽  
Author(s):  
Alisson Fernando Chiorato ◽  
Sérgio Augusto Morais Carbonell ◽  
Luciana Lasry Benchimol ◽  
Marilia Barbosa Chiavegato ◽  
Luiz Antonio dos Santos Dias ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Common Bean ◽  
Genetic Similarity ◽  
Germplasm Conservation ◽  
Molecular Data ◽  
Ex Situ ◽  
Germplasm Bank ◽  
Improvement Programs ◽  
Improved Cultivars ◽  
Germplasm Banks

Germplasm banks store genotype samples, improved varieties, landraces and wild species, all generically denominated accessions. The importance of characterizing germplasm banks is based on the identification and knowledge of relevant traits for genetic improvement and ex situ germplasm conservation. Thus, the present study had as aim the evaluation of the genetic diversity among 220 accessions of a Brazilian common bean germplasm bank of the "Instituto Agronômico de Campinas" (IAC) by means of 23 morpho-agronomical descriptors and 19 RAPD loci. These accessions correspond to genotypes from the Andean and Middle American gene pool as well as from cultivars derived from common bean improvement programs. The Middle American accessions and the improved cultivars were clustered into one group, distinct from the one formed by the Andean accessions. In relation to the molecular data, 47% of the genetic similarity was detected among the Middle American accessions, and similar results were observed for the improved cultivars (50%). The Andean accessions revealed 60% of genetic similarity. The cluster constituted by the improved cultivars and the Middle American genotypes differed, basically, in tegument color. Both molecular and morpho-agronomical data sets were equally effective to quantify and organize the genetic diversity of common bean accessions. This information may be useful to direct crosses and for the proper organization of the IAC germplasm bank.

scholarly journals Genetic characterization of the Moxotó goat breed using RAPD markers

2005 ◽  
Vol 40 (3) ◽  
pp. 233-239 ◽  
Author(s):  
Rejane Rodrigues de Oliveira ◽  
Andréa Alves do Egito ◽  
Maria Norma Ribeiro ◽  
Samuel Rezende Paiva ◽  
Maria do Socorro Maués Albuquerque ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Rapd Markers ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Distances ◽  
Goat Breed ◽  
Breeding Programs ◽  
Analysis Of Molecular Variance ◽  
Polymorphic Bands

The objective of this study was to verify the genetic diversity between and within seven populations of Moxotó goat (n = 264) from the States of Pernambuco, Paraíba and Rio Grande do Norte, using RAPD (Random Amplified Polymorphic DNA). Moxotó, as well as other naturalized breeds, suffers genetic losses due to the indiscriminate miscegenation with breeds raised in the Northeast Region of Brazil. The genetic characterization of these genetic resources is essential to conservation and breeding programs. DNA was extracted from lymphocytes using a non-organic protocol. The 16 primers used were selected from 120 decamer oligonucleotide primers and generated 56 polymorphic bands. The analysis of molecular variance (AMOVA) showed that the greater part of total genetic variability (71.55%) was due to differences between individuals within populations, while 21.21% was among populations. The analysis of variance among the pairs of populations demonstrated that the populations located in Floresta, PE x Angicos, RN presented a smaller value of intrapopulational differentiation (8.9%), indicating low genetic variability among them. Nei's genetic distances varied between 0.0546 and 0.1868 in the populations. The dendrogram generated showed that the Canindé breed, used as outgroup, clustered with the populations of Moxotó, indicating a possible common origin of the naturalized goat breeds.

scholarly journals Chemical characterization of an Ipecac active germplasm bank – a Brazilian endangered medicinal species

2021 ◽  
Vol 10 (15) ◽  
Author(s):  
Nádia Elígia Nunes Pinto Paracampo ◽  
Luiz Ricardo Santos de Souza ◽  
Jéssica Caporal Almeida ◽  
Osmar Alves Lameira
Keyword(s):  
High Performance ◽  
Genetic Material ◽  
Chemical Characterization ◽  
Array Detector ◽  
Germplasm Bank ◽  
Medicinal Species ◽  
Breeding Programs ◽  
The City ◽  
Selection Of

The active germplasm bank (AGB) of Carapichea ipecacuanha (Brot.) L. Andersson at Embrapa Eastern Amazon, in the city of Belém, PA, was the first of its kind to be opened in Brazil for this endangered medicinal species and holds important accessions for agricultural and reproductive research, including the production of active principles. This study aimed to chemically characterize 42 accessions from that AGB by simultaneously quantifying cephaeline and emetine contents in roots using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Cephaeline concentrations ranged from ‘undetected’ to 1.76%, whereas emetine concentrations were found between 0.64% and 2.49%. The overall emetine/cephaeline ratio varied from 0.43 to 3.52. The differences among mean concentrations of alkaloids observed by comparing the Scott-Knott test at 5% probability suggest the chemical variation among the samples assessed. Therefore, it is concluded such chemical differences may favor the selection of genetic material for commercial purposes based on the production of emetine and/or cephaeline, likewise may contribute to breeding programs.

scholarly journals Phenological diversity in a World Olive Germplasm Bank: Potential use for breeding programs and climate change studies

2020 ◽  
Vol 18 (1) ◽  
pp. e0701 ◽  
Author(s):  
Angjelina Belaj ◽  
Raúl De la Rosa ◽  
Lorenzo León ◽  
Clara Gabaldón-Leal ◽  
Cristina Santos ◽  
...  
Keyword(s):  
Climate Change ◽  
Simulation Models ◽  
Relevant Information ◽  
Adaptation Strategies ◽  
Germplasm Bank ◽  
Breeding Programs ◽  
Germplasm Collections ◽  
Cultivar Selection ◽  
Full Flowering

Aim of study: Crop phenology is a critical component in the identification of impacts of climate change. Then, the assessment of germplasm collections provides relevant information for cultivar selection and breeding related to phenology, being the base for identifying adaptation strategies to climate change.Area of study: The World Olive Germplasm Bank located at IFAPA Centre “Alameda del Obispo” (WOGB-IFAPA) in Cordoba (Southern Spain) was considered for the study.Material and methods: Data gathered for nine years on flowering and ripening time of olive cultivars from WOGB-IFAPA were evaluated. Thus, full flowering date (FFD) for 148 cultivars and ripening date (RD) for 86 cultivars, coming from 14 olive growing countries, were considered for characterization of olive phenology and for calibration/validation of phenological models.Main results: The characterization of WOGB-IFAPA has allowed the identification of cultivars with extreme early (‘Borriolenca’) and late (‘Ulliri i Kuq’) flowering as well as the ones with extreme early (‘Mavreya’) and late (‘Gerboui’) ripening dates. However, the very limited inter-cultivar variability, especially for FFD, resulted in a non-optimal simulation models performance. Thus, for FFD and RD the root mean square error was around 6 and 24 days, respectively. The limited inter-cultivar variability was associated to the low average temperatures registered during winter at WOGB-IFAPA generating an early accumulation of the chilling requirements, thus homogenizing FFD of all the analyzed cultivars.Research highlights: The identification of cultivars with early FFD and late RD provides useful information for breeding programs and climate change studies for identifying adaptation strategies.

Genetic variability of three fish species of genus Puntius

2017 ◽  
Vol 2 (2) ◽  
pp. 153
Author(s):  
Abu-Almaaty A. H. ◽  
Iman M. Bahgat ◽  
Zaineb M. Al-Tahr
Keyword(s):  
Molecular Weight ◽  
Genetic Variation ◽  
Genetic Variability ◽  
Fish Species ◽  
Genetic Similarity ◽  
Molecular Detection ◽  
Electrophoretic Analysis ◽  
Sds Page ◽  
New Varieties ◽  
Issr Primers

The present study was aimed to evaluate the genetic variability among three fish species of genus Puntius, Puntius semifasciolatus, Puntius conchonius and Puntius oligolepis (family: Cyprinidae) using various genetic markers (SDS-Page and ISSR). The electrophoretic analysis of protein was revealed 29 bands of molecular weight ranging from 11 to 132KD with polymorphism 28%. A total of eleven ISSR primers produced 123 total bands their size ranged from 79 to 1185bp and the percentage of polymorphism ranged from 91% to 100%. Results analysis showed that the percentages of genetic relationship among three species were 33% and 47%. The results of the study revealed that Puntius semifasciolatus and Puntius conchonius have higher genetic similarity 47% while Puntius semifasciolatus and Puntius oligolepis exhibited lower genetic similarity 33%. Considering all the gained data, it is evident that molecular detection of SDS-Page and ISSR are suitable tools in assessing genetic variation among fish species. These markers provide interesting tools for breeding new varieties of Egyptian fish.

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