Changes in mRNA and Protein Expression of Antioxidant Enzymes in Leukocytes of Children with Bronchial Asthma after Interval Hypoxic Training

2012 ◽  
Vol 3 (2) ◽  
pp. 125-134
Author(s):  
Klaudia V. Nesvitaylova ◽  
Olga A. Gonchar ◽  
Tatyana I. Drevitskaya ◽  
Ludmila P. Arabskaya ◽  
Mikhail M. Steshenko ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Bronchial Asthma ◽  
Hypoxic Training ◽  
Mrna And Protein Expression

scholarly journals Changes in mRNA and protein expression of antioxidant enzymes in children with bronchial asthma after interval hypoxic training

2012 ◽  
Vol 57 (6) ◽  
pp. 23-30 ◽  
Author(s):  
KV Nesvitaĭlova ◽  
◽  
OO Honchar ◽  
TI Drevyts'ka ◽  
LP Arabs'ka ◽  
...  
Keyword(s):  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Bronchial Asthma ◽  
Hypoxic Training ◽  
Mrna And Protein Expression

scholarly journals Proanthocyanidins Alleviates AflatoxinB1-Induced Oxidative Stress and Apoptosis through Mitochondrial Pathway in the Bursa of Fabricius of Broilers

Toxins ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 157 ◽  
Author(s):  
Shahid Rajput ◽  
Cong Zhang ◽  
Yue Feng ◽  
Xiao Wei ◽  
Mahmoud Khalil ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Basal Diet ◽  
Mitochondrial Pathway ◽  
Bursa Of Fabricius ◽  
Enzymes Activities ◽  
Study Results ◽  
Mrna And Protein Expression ◽  
Antioxidant Enzymes Activities

Aflatoxin B1 (AFB1) is a serious threat to the poultry industry. Proanthocyanidins (PCs) demonstrates a broad range of biological, pharmacological, therapeutic, and chemoprotective properties. The aim of this study was to investigate the ameliorative effects of PCs against AFB1-induced histopathology, oxidative stress, and apoptosis via the mitochondrial pathway in the bursa of Fabricius (BF) of broilers. One hundred forty-four one-day old Cobb chicks were randomly assigned into four treatment groups of six replicates (6 birds each replicate) for 28 days. Groups were fed on the following four diets; (1) Basal diet without addition of PCs or AFB1 (Control); (2) basal diet supplemented with 1 mg/kg AFB1 from contaminated corn (AFB1); (3) basal diet supplemented with 250 mg/kg PCs (PCs); and (4) basal diet supplemented with 1 mg/kg AFB1 + 250 mg/kg PCs (AFB1+ PCs). The present study results showed that antioxidant enzymes activities of total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione S-transferase (GST) in AFB1 treated group were (p < 0.05) decreased, whereas malondialdehyde (MDA) contents were significantly increased in comparison with the control group. Furthermore, we found that dietary PCs treatment ameliorated AFB1-induced oxidative stress in the BF through inhibiting the accumulation of MDA content and enhancing the antioxidant enzymes activities (T-SOD, CAT, GSH-Px, and GST). Similarly, PCs markedly enhanced messenger RNA (mRNA) expression of antioxidant genes (SOD, CAT, GPx1, and GST) in comparison with AFB1 group. Moreover, histological results showed that PCs alleviated AFB1-induced apoptotic cells in the BF of broilers. In addition, both mRNA and protein expression results manifested that mitochondrial-apoptosis-associated genes (Bax, caspase-9, caspase-3, and p53 and cytochrome c) showed up-regulation, while (Bcl-2) showed down-regulation in AFB1 fed group. The supplementation of PCs to AFB1 diet significantly reversed the mRNA and protein expression of these apoptosis-associated genes, as compared to the AFB1 group. Our results demonstrated that PCs ameliorated AFB1-induced oxidative stress by modulating the antioxidant defense system and apoptosis in the BF through mitochondrial pathway in broilers.

scholarly journals Hepatic Postnatal Developmental Expression of Trace Mineral Associated Antioxidant Enzymes

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1077-1077
Author(s):  
Laura Sherlock ◽  
Kara Sjostrom ◽  
Nancy Krebs ◽  
Clyde Wright ◽  
Eva Nozik-Grayck
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Protein Expression ◽  
Developmental Regulation ◽  
Immune Surveillance ◽  
Developmental Expression ◽  
Antioxidant Defenses ◽  
Trace Mineral ◽  
Mrna And Protein Expression ◽  
Neonatal Liver

Abstract Objectives Oxidative stress is central to the etiology of many diseases of prematurity. Lower antioxidant defenses render premature infants vulnerable to oxidative damage secondary to infection and oxygen therapy. Antioxidant enzymes (AOE) increase perinatally in the blood and lungs. Many AOE require a micronutrient such as selenium (Se) or zinc (Zn) to function at maximum efficacy. These trace elements are low in neonates compared to adults. The liver is an important immune surveillance organ where antioxidant defense is critical for host response. It also plays a major role in micronutrient processing. However, the developmental regulation and expression of AOE in the liver is incompletely described. We hypothesized the neonatal liver would have decreased trace mineral associated AOE. Methods C57BL/6mice were sacrificed at P0, P7, P21 and 8–12 weeks. mRNA and protein expression of key AOE (SOD1, SOD2, SOD3, Gpx1, Gpx4, Msrb1, TrxR1) and factors for Se processing (Sephs2/Sps2, Scly, Pstk) were measured by qPCR and Western blot. Results Hepatic mRNA for selenoenzymes Gpx1 and Msrb1 were developmentally regulated, low at P0 and increased by adult (P &lt; 0.05, n = 5–6). Gpx1 protein increased 7–8-fold and Msrb1 protein increased 6-fold from P0 to adult (P &lt; 0.0001, n = 4). Gene expression of Zn related SOD1 and Mn SOD2 increased postnatally, low at P0 and increased in adult (P &lt; 0.01 n = 5–6). Protein expression for each increased 1.5 and 3-fold from P0 to adult respectively (P &lt; 0.001, n = 4) The mRNA and protein expression for Gpx4, TrxR1 and SOD3 remained constant postnatally. As the greatest increase was observed in selenoenzymes, factors for Se processing were evaluated. Sephs2, Scly and Pstk mRNA increased from P0 compared to P21 and adult mice (P &lt; 0.05, n = 4–6). Protein expression for Pstk and Scly was highest at P21 and protein for Sps2 increased postnatally (P &lt; 0.01, n = 4). Conclusions The liver experiences a postnatal increase in essential trace mineral associated AOE. Additionally, the hepatic machinery for Se processing is low in neonatal mice. We speculate that the neonatal liver is vulnerable to oxidative stress secondary to low AOE defense. We also speculate states that decreased neonatal micronutrient status may further impair the hepatic redox state. Funding Sources CCTSI Child Maternal Health Mentored Grant (L.S).

Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients

2000 ◽  
Vol 111 (4) ◽  
pp. 1118-1121 ◽  
Author(s):  
A. Bellahcene ◽  
I. Van Riet ◽  
C. de Greef ◽  
N. Antoine ◽  
M. F. Young ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Protein Expression ◽  
Cell Lines ◽  
Myeloma Cell ◽  
Bone Sialoprotein ◽  
Multiple Myeloma Cell ◽  
Human Multiple Myeloma ◽  
Mrna And Protein Expression

Icariin Stimulates hFOB 1.19 Osteoblast Proliferation and Differentiation via OPG/RANKL Mediated by the Estrogen Receptor

2020 ◽  
Vol 22 (1) ◽  
pp. 168-175 ◽  
Author(s):  
Lin-Jun Sun ◽  
Chong Li ◽  
Xiang-hao Wen ◽  
Lu Guo ◽  
Zi-Fen Guo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Estrogen Receptor ◽  
Protein Expression ◽  
Chinese Herb ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Expression Ratio ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Mrna And Protein Expression

Background:: Icariin (ICA), one of the main effective components isolated from the traditional Chinese herb Epimedium brevicornu Maxim., has been reported to possess extensive pharmacological actions, including enhanced sexual function, immune regulation, anti-inflammation, and antiosteoporosis. Methods:: Our study was designed to investigate the effect of ICA on cell proliferation and differentiation and the molecular mechanism of OPG/RANKL mediated by the Estrogen Receptor (ER) in hFOB1.19 human osteoblast cells. Results:: The experimental results show that ICA can stimulate cell proliferation and increase the activity of Alkaline Phosphatase (ALP), Osteocalcin (BGP) and I Collagen (Col I) and a number of calcified nodules. Furthermore, the mRNA and protein expression of OPG and RANKL and the OPG/ RANKL mRNA and protein expression ratios were upregulated by ICA. The above-mentioned results indicated that the optimal concentration of ICA for stimulating osteogenesis was 50ng/mL. Subsequent mechanistic studies comparing 50ng/mL ICA with an estrogen receptor antagonist demonstrated that the effect of the upregulated expression is connected with the estrogen receptor. In conclusion, ICA can regulate bone formation by promoting cell proliferation and differentiation and upregulating the OPG/RANKL expression ratio by the ER in hFOB1.19 human osteoblast cells.

Effects of Morphine on Interstitial Cells of Cajal in Rabbit Colon and Small Intestinal Transit: An Experimental Study

2020 ◽  
Vol 20 (3) ◽  
pp. 240-246
Author(s):  
Heng Yang ◽  
Xiao-Ju Jin ◽  
Hong Luo ◽  
Yuan-Hai Li
Keyword(s):  
Protein Expression ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Saline Control ◽  
Morphine Group ◽  
Small Intestinal Transit ◽  
Medium Concentration ◽  
Epidural Puncture ◽  
Mrna And Protein Expression ◽  
Cells Of Cajal

Objective: This study aims to investigate the effect of morphine with naloxone on intestinal peristalsis and the number of interstitial cells of Cajal (ICC) in colon tissues of rabbits. Methods: Thirty rabbits were randomly divided into five groups (n=6, each group): saline control group (NS group), low concentration of morphine group (L group), medium concentration of morphine group (M group), high concentration of morphine group (H group), medium concentration of morphine and naloxone mixed with antagonist group (NM group). Rabbits in these five groups were administered with an epidural puncture tube and dorsal epidural analgesia pump, and were continuously infused for seven days. Fecal characteristics were observed, and the ink propulsion rate was calculated. The expression level of ICC C-kit protein in colon tissues was tested by western blot. Results: The stool characteristics in the L, M and H groups were more severe than those in the NS and NM groups. Furthermore, the intestinal propulsion rate in the L, M and H groups was lower than that in the NS and NM groups. The C-kit mRNA and protein expression in the colon of rabbits were significantly lower in the L, M and H groups, when compared to the NS and NM groups. Conclusions: Naloxone blocked the mRNA and protein expression of C-kit, and improved intestinal motor function.

scholarly journals Upregulation of ETV2 Expression Promotes Endothelial Differentiation of Human Dental Pulp Stem Cells

2021 ◽  
Vol 30 ◽  
pp. 096368972097873
Author(s):  
Jing Li ◽  
Youming Zhu ◽  
Na Li ◽  
Tao Wu ◽  
Xianyu Zheng ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Tube Formation ◽  
Endothelial Differentiation ◽  
Cell Source ◽  
Lentiviral Infection ◽  
Mrna And Protein Expression ◽  
Human Dental Pulp

The lack of vasculogenesis often hampers the survivability and integration of newly engineered tissue grafts within the host. Autologous endothelial cells (ECs) are an ideal cell source for neovascularization, but they are limited by their scarcity, lack of proliferative capacity, and donor site morbidity upon isolation. The objective of this study was to determine whether differentiation of human dental pulp stem cells (DPSCs) into the endothelial lineage can be enhanced by recombinant ETV2 overexpression. DPSCs were extracted from fresh dental pulp tissues. ETV2 overexpression in DPSCs was achieved by lentiviral infection and cellular morphological changes were evaluated. The mRNA and protein expression levels of endothelial-specific markers were assessed through quantitative real-time polymerase chain reaction, western blot, immunofluorescence staining, and flow cytometry. The tube formation assay and Matrigel plug assay were also performed to evaluate the angiogenic potential of the ETV2-transduced cells in vitro and in vivo, respectively. Additionally, proteomic analysis was performed to analyze global changes in protein expression following ETV2 overexpression. After lentiviral infection, ETV2-overexpressing DPSCs showed endothelial-like morphology. Compared with control DPSCs, significantly higher mRNA and protein expression levels of endothelial-specific genes, including CD31, VE-Cadherin, VEGFR1, and VEGFR2, were detected in ETV2-overexpressing DPSCs. Moreover, ETV2 overexpression enhanced capillary-like tube formation on Matrigel in vitro, as well as neovascularization in vivo. In addition, comparative proteomic profiling showed that ETV2 overexpression upregulated the expression of vascular endothelial growth factor (VEGF) receptors, which was indicative of increased VEGF signaling. Taken together, our results indicate that ETV2 overexpression significantly enhanced the endothelial differentiation of DPSCs. Thus, this study shows that DPSCs can be a promising candidate cell source for tissue engineering applications.

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