Inhibitory effect of Gardenblue blueberry (Vaccinium ashei Reade) anthocyanin extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 cells

AbstractDoxorubicin (DOX) chemotherapy is associated with the release of inflammatory cytokines from macrophages. This has been suggested to be, in part, due to DOX-mediated leakage of endotoxins from gut microflora, which activate Toll-like receptor 4 (TLR4) signaling in macrophages, causing severe inflammation. However, the direct function of DOX on macrophages is still unknown. In the present study, we tested the hypothesis that DOX alone is incapable of stimulating inflammatory response in macrophages. Then, we compared the anti-inflammatory effects of curcumin (CUR), resveratrol (RES) and sulforaphane (SFN) against lipopolysaccharide/interferon-gamma (LPS/IFN-γ)-mediated inflammation in the absence or presence of DOX. For this purpose, RAW 264.7 cells were stimulated with LPS/IFN-γ (10 ng/mL/10 U/mL) in the absence or presence of DOX (0.1 µM). Our results showed that DOX alone is incapable of stimulating an inflammatory response in RAW 264.7 macrophages. Furthermore, after 24 h of incubation with LPS/IFN-γ, a significant increase in tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) mRNA levels was observed. Similarly, nitric oxide (NO) production and TNF-α and IL-6 protein levels were significantly upregulated. Moreover, in LPS/IFN-γ-treated macrophages, the microRNAs (miRNAs) miR-146a, miR-155, and miR-21 were significantly overexpressed. Interestingly, upon testing CUR, RES, and SFN against LPS/IFN-γ-mediated inflammation, only SFN was able to significantly reverse the LPS/IFN-γ-mediated induction of iNOS, TNF-α and IL-6 and attenuate miR-146a and miR-155 levels. In conclusion, SFN, at the transcriptional and posttranscriptional levels, exhibits potent immunomodulatory action against LPS/IFN-γ-stimulated macrophages, which may indicate SFN as a potential treatment for DOX-associated inflammation.

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Saikosaponin A mediates the inflammatory response by inhibiting the MAPK and NF-κB pathways in LPS-stimulated RAW 264.7 cells

Experimental and Therapeutic Medicine ◽

10.3892/etm.2013.988 ◽

2013 ◽

Vol 5 (5) ◽

pp. 1345-1350 ◽

Cited By ~ 53

Author(s):

JIE ZHU ◽

CHENGQUN LUO ◽

PING WANG ◽

QUANYONG HE ◽

JIANDA ZHOU ◽

...

Keyword(s):

Inflammatory Response ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Saikosaponin A

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Anti-Inflammatory Effect of Lycii radicis in LPS-Stimulated RAW 264.7 Macrophages

The American Journal of Chinese Medicine ◽

10.1142/s0192415x14500566 ◽

2014 ◽

Vol 42 (04) ◽

pp. 891-904 ◽

Cited By ~ 11

Author(s):

Mi Young Song ◽

Hyo Won Jung ◽

Seok Yong Kang ◽

Kyung-Ho Kim ◽

Yong-Ki Park

Keyword(s):

Inflammatory Cytokines ◽

Inflammatory Mediators ◽

Water Extract ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Root Bark ◽

Lycium Barbarum ◽

Anti Inflammatory ◽

Inflammatory Effect

The root bark of Lycium barbarum (Lycii radicis cortex, LRC) is used as a cooling agent for fever and night sweats in East Asian traditional medicine. The inhibitory effect of LRC water extract on inflammation is unknown. In this study, the anti-inflammatory effect of LRC was investigated in lipopolysaccharide (LPS)-stimulated mouse macrophage, RAW 264.7 cells. LRC extract significantly decreased the LPS-induced production of inflammatory mediators, nitric oxide (NO), prostaglandin (PG) E2 and pro-inflammatory cytokines, interleukin (IL)-1β and IL-6 in the cells. In addition, LRC extract inhibited the LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 mRNA and protein, and inflammatory cytokines mRNA in the cells. The action mechanism of LRC underlies the blocking of LPS-mediated p38 and Jun N-terminal kinase (JNK), mitogen-activated protein kinases (MAPKs), and the nuclear factor (NF)-κB signaling pathway. These results indicate that LRC extract inhibits the inflammatory response in activated macrophages by down-regulating the transcription levels of inflammatory mediators and blocking the MAPKs and NF-κB pathway.

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Inhibitory Effect of Sambucus sieboldiana var. pendula (Nakai) Extract on the mRNA and Protein Expression of iNOS and COX-2 in Raw 264.7 Cells

Microbiology and Biotechnology Letters ◽

10.4014/mbl.1704.04002 ◽

2017 ◽

Vol 45 (2) ◽

pp. 178-183 ◽

Cited By ~ 1

Author(s):

jin-Young Lee ◽

Dan-Hee Yoo ◽

Jung-Woo Chae

Keyword(s):

Protein Expression ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Mrna And Protein Expression ◽

Cox 2

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Esculentoside B inhibits inflammatory response through JNK and downstream NF-κB signaling pathway in LPS-triggered murine macrophage RAW 264.7 cells

International Immunopharmacology ◽

10.1016/j.intimp.2019.01.003 ◽

2019 ◽

Vol 68 ◽

pp. 156-163 ◽

Cited By ~ 5

Author(s):

Fukushi Abekura ◽

Junyoung Park ◽

Choong-Hwan Kwak ◽

Sun-Hyung Ha ◽

Seung-Hak Cho ◽

...

Keyword(s):

Inflammatory Response ◽

Signaling Pathway ◽

Raw 264.7 Cells ◽

Raw 264.7 ◽

Murine Macrophage

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Purification, characterization, crystal structure and NO production inhibitory activity of three new sesquiterpenoids from homalomena occulta

Acta Crystallographica Section C Structural Chemistry ◽

10.1107/s2053229618013815 ◽

2018 ◽

Vol 74 (11) ◽

pp. 1440-1446

Author(s):

Qi Zhang ◽

Li Ma ◽

Zhaoxia Qu ◽

Guige Hou ◽

Yanan Wang ◽

...

Keyword(s):

Crystal Structure ◽

Single Crystal ◽

2D Nmr ◽

Inhibitory Effect ◽

Raw 264.7 Cells ◽

No Production ◽

Raw 264.7 ◽

X Ray Diffraction ◽

X Ray ◽

Spectroscopic Analyses

Two new isodaucane-type sesquiterpenoids, namely (1R,4S,5S,6R,7S,10R)-isodauc-6,7,10-triol, C15H28O3, (1), and (1R,4S,5S,6S,7S,10R)-isodauc-6,7,10-triol, (2), and a new eudesmane-type sesquiterpenoid, 1β,4β,5α-trihydroxyeudesmane, (3), were obtained from the rhizomes of homalomena occulta with the aid of column chromatography. Their structures were elucidated based on extensive spectroscopic analyses, including 1D NMR, 2D NMR and HRESIMS. The structure of (1) was confirmed by single-crystal X-ray diffraction and the absolute configuration was assigned with respect to that of the precursor. The single-crystal structure reveals that adjacent molecules of (1) embrace through two groups of intermolecular O—H...O hydrogen bonds to generate a two-dimensional sheet with a 63-net topology. The three compounds were evaluated for their activity against lipopolysaccharide-induced production of nitrogen oxide (NO) in RAW 264.7 cells, and (1) showed an inhibitory effect on NO production, with IC50 values of 5.7±0.22 µM.

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