Experiments using microarray technology: limitations and standard operating procedures

Microarrays are a powerful method for the global analysis of gene or protein content and expression, opening up new horizons in molecular and physiological systems. This review focuses on the critical aspects of acquiring meaningful data for analysis following fluorescence-based target hybridisation to arrays. Although microarray technology is adaptable to the analysis of a range of biomolecules (DNA, RNA, protein, carbohydrates and lipids), the scheme presented here is applicable primarily to customised DNA arrays fabricated using long oligomer or cDNA probes. Rather than provide a comprehensive review of microarray technology and analysis techniques, both of which are large and complex areas, the aim of this paper is to provide a restricted overview, highlighting salient features to provide initial guidance in terms of pitfalls in planning and executing array projects. We outline standard operating procedures, which help streamline the analysis of microarray data resulting from a diversity of array formats and biological systems. We hope that this overview will provide practical initial guidance for those embarking on microarray studies.

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ANALISIS PROSEDUR ADMINISTRASI PENJUALAN TUNAI DAN KREDIT PADA PT SUN STAR MOTOR MALANG GUNA MENINGKATKAN PENGENDALIAN INTERN

Adbis: Jurnal Administrasi dan Bisnis ◽

10.33795/j-adbis.v13i2.77 ◽

2020 ◽

Vol 13 (2) ◽

pp. 184

Author(s):

Shinta Maharani Trivena ◽

Rena Feri Wijayanti ◽

Tri Yulistyawati Evelina

Keyword(s):

Data Analysis ◽

Data Processing ◽

Internal Control ◽

Descriptive Study ◽

Qualitative Approach ◽

Standard Operating Procedures ◽

Analysis Techniques ◽

Processing Data ◽

Standard Operating

The purpose of this study was to determine the application of cash and credit sales administration at PT Sun Star Motor Malang in accordance with the existing Standard Operating Procedures. This type of research used in this study is a descriptive study with a qualitative approach. Data analysis techniques by finding and finding data, eliminating or reducing data, processing data, analyzing data, making conclusions and suggestions. The result is that the application of cash sales administration procedures at PT Sun Star Motor Malang is sometimes not in accordance with existing procedures. This is due to the accumulation of tasks performed by sales because of the quite important role of sales for consumers. If it is related to internal control, sales should not be able to accept cash because every sales transaction related to receiving cash is held by the cashier. Every receipt of money, both cash and non-cash from the buyer, will be made proof of receipt, for example receipts

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PEMBERDAYAAN PERAJIN INDUSTRI ROTAN PASCA BENCANA OLEH DINAS PERDAGANGAN DAN PERINDUSTRIAN KOTA PALU

J-3P (Jurnal Pembangunan Pemberdayaan Pemerintahan) ◽

10.33701/j-3p.v5i1.1077 ◽

2020 ◽

pp. 19-32

Author(s):

Wilda Maulidia ◽

Hasna Azmi Fadhilah ◽

Hendrawati Hamid

Keyword(s):

Raw Materials ◽

Standard Operating Procedures ◽

City Government ◽

Data Presentation ◽

Analysis Techniques ◽

Standard Operating ◽

The Government ◽

The City ◽

Central Sulawesi ◽

And Training

This research titled is “Empowerment of Rattan Industry Craftsmen After Disasters by Department of Trade and Industry in Palu City Central Sulawesi Province”. The purpose of this research is to find out and analyze the empowerment process, supporting factors and inhibiting factors faced, as well as efforts made by the Department of Trade and Industry to overcome obstacles in the empowerment of rattan industry crafters in the aftermath of a disaster. The research method used by the author in this study is descriptive qualitative.The data collection techniques are interviews, documens and observations. The data analysis techniques in this study are data reduction, data presentation and data verification. The result of this research apprenticeship show that empowerment of crafters in managing the rattan industry after disaster has been carried out. But in the implementation, the empowerment process has not been carried out optimally. Because the mindset of craftsmen who are still using old mindset, the absence of Standard Operating Procedures regarding the rattan industry which results in an ineffective upstream and downstream system, Limited funds owned by the government in providing assistance and the independence of the craftsmen in producing raw materials supporting the rattan industry. After disaster, Department of Trade and Industry has made efforts to overcome obstacles with : make a Standard Operating Procedures dan rebuilding the upstream and downstream systems. In addition, Department of Trade and Industry has also allocated funds in accordance with needs and provides socialization in changing the mindset of rattan industry crafters and training to develop rattan industry businesses. The authors suggests to the city government that in the future in making a development and empowerment planning, the city government must fist make Standard Operating Procedures. As well as the process of the rehabilitation and reconstruction stages, the government must better understand its tasks and functions Keyword : Empowerment, Craftsmen, Rattan Industry, Disaster

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“An Effective Multidimensional Model for Analyzing Social Web Big Data – Testing in simple Web 2.0 Applications of Internet Politics”

Communications of the IBIMA ◽

10.5171/2021.589003 ◽

2021 ◽

pp. 1-18

Author(s):

Dimitrios VAGIANOS ◽

Kostas ZAFIROPOULOS

Keyword(s):

Big Data ◽

Web 2.0 ◽

Social Web ◽

Multidimensional Model ◽

New Horizons ◽

Analysis Techniques ◽

Internet Politics ◽

Opening Up

Web 2.0 applications have provided researchers with vast quantities of Big Data, opening up new horizons for developing innovative analysis techniques that are applicable in multiple cognitive fields. Politics is definitely among them and is currently in a dynamically evolving range of these applications.

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ANALISA PENGARUH SOP ( STANDARD OPERATING PROCEDURE ) TERHADAP KINERJA WAITER DAN WAITRESS DI IN BLOOM RESTORAN HOTEL AYAARTTA MALIOBORO YOGYAKARTA, INDONESIA

Journal of Tourism and Economic ◽

10.36594/jtec.v4i1.112 ◽

2021 ◽

Vol 4 (1) ◽

pp. 73-84

Author(s):

Slamet Supriyanto ◽

Lutfi Hendriyati

Keyword(s):

Statistical Analysis ◽

Operating Procedure ◽

Standard Operating Procedure ◽

Standard Operating Procedures ◽

Quantitative Approach ◽

Work Activities ◽

Analysis Techniques ◽

Standard Operating ◽

Approach Method ◽

Using Data

Standard operating procedures or commonly called SOPs are important things that every company must have and must be a guide in carrying out work activities by all waiters and waiters at In Bloom Restaurant Hotel Ayaartta Malioboro Yogyakarta. This research uses a quantitative approach method, using data from questionnaires and uncovering problems in the form of numerical scores, which are then processed and tested by statistical analysis techniques in an effort to reveal the performance and effectiveness of SOP implementation in carrying out tasks at Bloom's Restaurant.

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Management of oxygen saturation monitoring in preterm newborns in the NICU: the Italian picture

Italian Journal of Pediatrics ◽

10.1186/s13052-021-01050-3 ◽

2021 ◽

Vol 47 (1) ◽

Author(s):

Serafina Perrone ◽

◽

Maurizio Giordano ◽

Giuseppe De Bernardo ◽

Paola Lugani ◽

...

Keyword(s):

Oxygen Saturation ◽

Healthcare Professionals ◽

Cross Sectional Study ◽

Standard Operating Procedures ◽

Specific Training ◽

Related Factors ◽

Cross Sectional ◽

Survey Form ◽

Standard Operating ◽

Oxygen Monitoring

Abstract Background Although many studies emphasize the importance of using oxygen saturation (SpO2) targets in the NICUs, there is a wide variability in used saturation ranges among centers. Primary aim was to draw a representative picture on how the management of oxygen monitoring is performed in the Italian NICUs. Second aim was to identify healthcare-professionals related factors associated with oxygen targeting in the preterm population. Methods Cross-sectional study with data collection via an electronic survey form. A questionnaire containing pre-piloted and open questions on monitoring and management of the SpO2 was administered to neonatologists across the network of the Italian Society of Neonatology. The questions focused on: the infrastructure, specific training, healthcare professionals and patients-related factors. The results of the survey were anonymously collected, summarized and analyzed. Results Out of 378 questionnaires, 93 were correctly filled. Thirty-six different SpO2 ranges were observed. Centers using written standard operating procedures on oxygen management and SpO2 monitoring maintained a correct average range of SpO2 90–95%, avoided hyperoxia and reconsidered saturation targets in relation to comorbidities. 39.8% of responders disabled alarms during neonatal care. One center used biomarkers for complete monitoring of neonatal oxygenation status. Conclusions There is considerable variation in SpO2 targets for preterm infants in the Italian NICUs. Standard operating procedures and specific training for health care personnel are the main factors playing a role for the correct maintenance of the recommended oxygen targets in preterms.

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O19 SBE, SOPs and COVID-19: Using simulation-based education (SBE) to facilitate the development of paediatric COVID-19 standard operating procedures (SOP)

10.1136/bmjstel-2020-aspihconf.19 ◽

2020 ◽

Author(s):

Frances Louise Hanlon ◽

Rosie Fish ◽

Emily Suckling ◽

Kirsty Brown

Keyword(s):

Standard Operating Procedures ◽

Simulation Based ◽

Standard Operating

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Improving Water Quality Can Reduce Pyrogenic Reactions Associated With Reuse of Cardiac Catheters

Infection Control and Hospital Epidemiology ◽

10.1086/502166 ◽

2003 ◽

Vol 24 (12) ◽

pp. 955-960 ◽

Cited By ~ 2

Author(s):

Rosemary E. Duffy ◽

Braulio Couto ◽

Jussara M. Pessoa ◽

Carlos Starling ◽

Silma Pinheiro ◽

...

Keyword(s):

Water Quality ◽

Cardiac Catheterization ◽

Linear Trend ◽

Phase 1 ◽

Standard Operating Procedures ◽

Chi Square ◽

Cardiac Catheters ◽

Standard Operating ◽

Chi Square Test ◽

Belo Horizonte

AbstractObjective:To report the results of our preintervention investigation and subsequent 19-month three-phase intervention study designed to reduce pyrogenic reactions among patients undergoing cardiac catheterization using reprocessed catheters.Design:A case-control study for the preintervention period and a prospective cohort study for the intervention period.Setting:A 400-bed hospital in Belo Horizonte, Brazil.Participants:Any patient undergoing cardiac catheterization in the hospital.Interventions:Three intervention phases were implemented to improve the quality of the water supplied to the cardiac catheter reprocessing laboratory. Standard operating procedures for reprocessing cardiac catheters were established and reprocessing staff were trained and educated.Results:The rate of pyrogenic reactions decreased significantly during the intervention phases, from 12.8% (159 of 1,239) in phase 1 to 5.3% (38 of 712) in phase 2 to 0.5% (4 of 769) in phase 3 (chi-square test for linear trend, 97.5;P< .001).Conclusion:Improving water quality and using standard operating procedures for reprocessing catheters can prevent pyrogenic reactions in hospitalized patients.

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