scholarly journals Resistance to Remnant Nephropathy in the Wistar-Furth Rat

1999 ◽  
Vol 10 (4) ◽  
pp. 814-821
Author(s):  
WAYNE R. FITZGIBBON ◽  
EDDIE L. GREENE ◽  
JASJIT S. GREWAL ◽  
FLORENCE N. HUTCHISON ◽  
SALLY E. SELF ◽  
...  
Keyword(s):  
Renal Injury ◽  
Renal Mass ◽  
Wistar Rats ◽  
Renal Damage ◽  
Mass Reduction ◽  
Mesangial Proliferation ◽  
Antihypertensive Regimen ◽  
Renal Ablation ◽  
Study Support ◽  
And Control

Abstract. The Wistar-Furth rat, an inbred strain resistant to actions of mineralocorticoids, was used to study the concept that mineralocorticoids contribute to progressive renal injury. It was postulated that if chronic nephropathy depends on aldosterone and if Wistar-Furth rats are resistant to aldosterone, remnant nephropathy would be attenuated in Wistar-Furth rats. Wistar-Furth rats and control Wistar rats were subjected to 5/6 nephrectomy or a sham procedure and then followed for 4 wk. Renal ablation resulted in hypertension at 4 wk in both strains (164 ± 5 [Wistar-Furth] versus 184 ± 7 [Wistar] mmHg mean arterial pressure), with sham animals remaining normotensive (134 ± 6 mmHg). Renal damage in response to 5/6 nephrectomy was greatly decreased in Wistar-Furth rats compared with Wistar rats. Albuminuria was markedly less in Wistar-Furth rats (12.7 ± 4.2 [Wistar-Furth] versus 97.4 ± 22.6 [Wistar] mg/d per 100 g body wt, P < 0.01). Glomerular damage, consisting of mesangial proliferation, mesangial lysis, and segmental necrosis, was observed in 42% of glomeruli from Wistar rats but in 0% of glomeruli from Wistar-Furth rats (P < 0.01). To address the possibility that higher BP in partially nephrectomized Wistar rats mediated the greater renal damage, the study was repeated, with Wistar rats (not Wistar-Furth rats) being treated with a hydralazine-reserpine-hydrochlorothiazide regimen. Although this antihypertensive regimen equalized BP (conscious systolic) (144 ± 8 mmHg [Wistar] versus 157 ± 7 mmHg [Wistar-Furth] at 4 wk), albuminuria remained more than 10-fold greater in Wistar rats. In summary, renal damage upon 5/6 nephrectomy was markedly reduced in Wistar-Furth rats, a finding not attributable to reduced systemic BP. Since Wistar-Furth rats have been shown previously to be resistant to the actions of mineralocorticoids, the data from the present study support the hypothesis that aldosterone mediates, at least in part, the renal injury attendant to renal mass reduction.

scholarly journals The Effects of Long-Term Chaetomellic Acid A Administration on Renal Function and Oxidative Stress in a Rat Model of Renal Mass Reduction

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
António Nogueira ◽  
Francisco Peixoto ◽  
Maria Manuel Oliveira ◽  
Carlos André Pires ◽  
Bruno Colaço ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Renal Mass ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Mass Reduction ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
And Oxidative Stress

Purpose.This study aimed to evaluate the effect of chronic treatment with chaetomellic acid A (CAA) on oxidative stress and renal function in a model of renal mass reduction.Methods. Male Wistar rats were subjected to 5/6 nephrectomy (RMR) or sham-operated (SO). One week after surgery, rats have been divided into four experimental groups: RMR: RMR rats without treatment(n=14); RMR + CAA: RMR rats treated with CAA(n=13); SO: SO rats without treatment(n=13); and SO + CAA: SO rats treated with CAA(n=13). CAA was intraperitoneally administered in a dose of 0.23 µg/Kg three times a week for six months.Results.RMR was accompanied by a significant reduction in catalase and glutathione reductase (GR) activity(p<0.05)and a decrease in reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio. CAA administration significantly increased catalase and GR activity(p<0.05)and increased GSH/GSSG ratio, but no significant difference between the treated and nontreated groups was found in this ratio. No significant differences were found between the RMR groups in any of the parameters of renal function. However, CAA administration slightly improves some parameters of renal function.Conclusions.These data suggest that CAA could attenuate 5/6 RMR-induced oxidative stress.

Angiotensin II control of the renal microcirculation in rats with reduced renal mass

1990 ◽  
Vol 258 (2) ◽  
pp. F414-F422 ◽  
Author(s):  
J. C. Pelayo ◽  
A. H. Quan ◽  
P. F. Shanley
Keyword(s):  
Renal Failure ◽  
Angiotensin Ii ◽  
Renal Mass ◽  
Wistar Rats ◽  
Sham Group ◽  
Ang Ii ◽  
Renal Ablation ◽  
Glomerular Hypertension ◽  
Single Nephron ◽  
Glomerular Ultrafiltration

It has been suggested that angiotensin II (ANG II) activation after renal ablation contributes to the altered glomerular dynamics and proteinuria that characterizes this model of chronic renal failure. In the present study, male Munich-Wistar rats underwent 75% renal ablation (Nx group). Two weeks later, micropuncture studies were performed in sham-operated rats (sham group) and Nx group rats during intravenous infusion of either a vehicle or two ANG II inhibitors, namely [Sar1, Ala8]ANG II or MK-421 administered at a rate of 0.3 and 1 mg.kg body wt-1.h-1, respectively. Acute ANG II inhibition in sham group had no effect on mean arterial pressure (MAP), glomerular dynamics, or proteinuria. In contrast, in Nx group ANG II inhibition lessened glomerular hypertension (from 64.7 +/- 1.0 to 55.4 +/- 1.7 mmHg, P less than 0.0001) the result of postglomerular vasodilation (P less than 0.01), normalized the glomerular ultrafiltration coefficient (from 0.038 +/- 0.002 to 0.005 +/- 0.002 nl.s-1.mmHg-1, P less than 0.0001), and attenuated proteinuria (from 42.1 +/- 6.5 to 28.1 +/- 5.4 micrograms/min, P less than 0.01). MAP, single-nephron GFR and plasma flow were unaffected. These results suggest that ANG II activity is enhanced in nephrectomy, contributing in a major way to altered glomerular dynamics and proteinuria.

Effect of Uninephrectomy on Urinary Prostaglandin E2, F2α, and 6-Ketoprostaglandin F1α Excretion in Rats

1984 ◽  
Vol 66 (4) ◽  
pp. 407-413 ◽  
Author(s):  
Philip G. Baer ◽  
Alberto Nasjletti
Keyword(s):  
Urinary Excretion ◽  
Renal Mass ◽  
Wistar Rats ◽  
Urine Volume ◽  
Urine Osmolality ◽  
Urinary Sodium Excretion ◽  
Functional Adaptation ◽  
Mass Reduction ◽  
Post Surgery ◽  
Male Wistar Rats

1. Male Wistar rats were used to examine whether renal prostaglandins (PG) contribute to functional compensatory changes in the remaining kidney after uninephrectomy. Total urinary excretion of PGE2, PGF2α and 6-keto-PGF1α, a metabolite of PGI2, was measured before and during 11 days after surgery in uninephrectomized (UNX, n = 8) and sham-operated control (S, n = 9) rats. 2. Urine volume was increased for the UNX rats on days 2–8 after surgery, but not thereafter, and urine osmolality for UNX was decreased on post-surgery days 1–9; total urinary sodium excretion did not differ between the two groups. Urinary PGE2 excretion was decreased in the UNX rats to 50% of S rat values, except on post-surgery days 2 and 3, when values for the two groups were not significantly different. Urinary PGF2α excretion by the UNX rats was reduced to 50% of S rat values throughout the post-surgery period. In contrast, the urinary excretion of 6-keto-PG1α by the UNX rats did not differ significantly from that by the S rats, except on a single day. 3. Chronic renal functional adaptation to renal mass reduction does not depend on increased renal prostaglandin systems activity; however, increased intrarenal PGE2 activity in the remaining kidney might be a factor in compensatory adjustments during the first days after surgery.

Melatonin ameliorates oxidative stress, inflammation, proteinuria, and progression of renal damage in rats with renal mass reduction

2008 ◽  
Vol 294 (2) ◽  
pp. F336-F344 ◽  
Author(s):  
Yasmir Quiroz ◽  
Atilio Ferrebuz ◽  
Freddy Romero ◽  
Nosratola D. Vaziri ◽  
Bernardo Rodriguez-Iturbe
Keyword(s):  
Oxidative Stress ◽  
Renal Failure ◽  
Renal Function ◽  
Chronic Renal Failure ◽  
Renal Mass ◽  
Mass Reduction ◽  
Melatonin Treatment ◽  
Melatonin Administration ◽  
Renal Ablation ◽  
Remnant Kidney

The progressive deterioration of renal function and structure resulting from renal mass reduction are mediated by a variety of mechanisms, including oxidative stress and inflammation. Melatonin, the major product of the pineal gland, has potent_antioxidant and anti-inflammatory properties, and its production is impaired in chronic renal failure. We therefore investigated if melatonin treatment would modify the course of chronic renal failure in the remnant kidney model. We studied rats followed 12 wk after renal ablation untreated (Nx group, n = 7) and treated with melatonin administered in the drinking water (10 mg/100 ml) (Nx + MEL group, n = 8). Sham-operated rats ( n = 10) were used as controls. Melatonin administration increased 13–15 times the endogenous hormone levels. Rats in the Nx + MEL group had reduced oxidative stress (malondialdehyde levels in plasma and in the remnant kidney as well as nitrotyrosine renal abundance) and renal inflammation (p65 nuclear factor-κB-positive renal interstitial cells and infiltration of lymphocytes and macrophages). Collagen, α-smooth muscle actin, and transforming growth factor-β renal abundance were all increased in the remnant kidney of the untreated rats and were reduced significantly by melatonin treatment. Deterioration of renal function (plasma creatinine and proteinuria) and structure (glomerulosclerosis and tubulointerstitial damage) resulting from renal ablation were ameliorated significantly with melatonin treatment. In conclusion, melatonin administration improves the course of chronic renal failure in rats with renal mass reduction. Further studies are necessary to define the potential usefulness of this treatment in other animal models and in patients with chronic renal disease.

scholarly journals ASCORBIC ACID;

2017 ◽  
Vol 24 (10) ◽  
pp. 1495-1500
Author(s):  
Aftab Ahmed Shaikh ◽  
Umair Ali Soomro ◽  
Shomail Saeed Siddiqui ◽  
Kashif Rasheed Shaikh ◽  
Mumtaz Ali Qureshi
Keyword(s):  
Ascorbic Acid ◽  
Renal Injury ◽  
Wistar Rats ◽  
Renal Tissue ◽  
Control Group ◽  
Male Wistar Rats ◽  
Biochemical Testing ◽  
Group B ◽  
Animal House ◽  
And Control

Objectives: Investigating the Renoprotective effects of Ascorbic acid ingentamicin induced renal injury (GIRI) in male Wistar rats. Study design: Experimental study.Place and Duration: Animal house of Al-Tibri from September 2016 to December 2016.Methodology: 60 male Wistar rats were selected by non-probability purposive sampling,and were divided into three groups; Group A- control, Group B- Gentamicin induced renalinjury (GIRI) (70 mg/kg/bwt i.m) daily , and Group C- GIRI + AA (Ascorbic acid - 0.2 mg/kg/bwt) daily. Cardiac puncture was performed by a Disposable Syringe for blood sampling.Sera were used for biochemical testing. Renal tissue was stained with H & E for histologicalexamination. Statistix 8.1 software (USA) was used for data analysis at P≤ 0.05. Results:Blood urea and serum creatinine were elevated in the group B (GIRI) compared to GIRI+AAand the controls (P=0.0001). Serum superoxide dismutase, glutathione peroxidase, andcatalase were low in GIRI compared to GIRI+AA and control groups. Renal tissue in ascorbicacid treated rats showed improved tissue architecture. Conclusion: It is concluded that theAscorbic acid exerts Renoprotective effects in gentamicin induced renal injury in rat model.

scholarly journals Delayed mTOR Inhibition with Low Dose of Everolimus Reduces TGFβ Expression, Attenuates Proteinuria and Renal Damage in the Renal Mass Reduction Model

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e32516 ◽  
Author(s):  
Melania Kurdián ◽  
Inmaculada Herrero-Fresneda ◽  
Nuria Lloberas ◽  
Pepita Gimenez-Bonafe ◽  
Virginia Coria ◽  
...  
Keyword(s):  
Renal Mass ◽  
Renal Damage ◽  
Low Dose ◽  
Mass Reduction ◽  
Mtor Inhibition ◽  
Reduction Model

Losartan-hydrochlorothiazide association promotes lasting blood pressure normalization and completely arrests long-term renal injury in the 5/6 ablation model

2007 ◽  
Vol 292 (6) ◽  
pp. F1810-F1818 ◽  
Author(s):  
Clarice Kazue Fujihara ◽  
Denise M. A. C. Malheiros ◽  
Roberto Zatz
Keyword(s):  
Blood Pressure ◽  
Drinking Water ◽  
Adult Male ◽  
Renal Injury ◽  
Wistar Rats ◽  
Long Period ◽  
Morphological Studies ◽  
Renal Ablation ◽  
Ablation Model

The possible long-term renoprotective effects of treatment with thiazides, either as monotherapy or associated with renin-angiotensin suppressors, have not been assessed. We investigated the effect of hydrochlorothiazide (H), alone or combined with losartan (L), in the 5/6 renal ablation model (Nx). Adult male Munich-Wistar rats underwent Nx, remaining untreated for 1 mo. At this time, functional and morphological studies were performed in 21 rats (group Nxpre). The remaining rats were distributed among groups: Nx, no treatment; Nx+L, receiving L, 50 mg·kg−1·day−1 in the drinking water; Nx+H, receiving H, 6 mg·kg−1·day−1 in drinking water; and Nx+L+H, receiving both L and H as described. At 30 days of treatment, systemic and glomerular pressures were markedly elevated in group Nx. Both H and L attenuated hypertension, whereas combined L+H treatment completely normalized both pressures. Eight months after Nx, mortality approached 70% in untreated rats, whereas severe albuminuria, hypertension, glomerulosclerosis, and interstitial expansion were observed. H and L attenuated, but did not prevent, mortality, hypertension, and renal injury. Combined L+H treatment completely prevented mortality, normalized albuminuria and blood pressure, and arrested renal injury at levels found 1 mo after ablation, despite the unusually long period of observation. Combined L+H treatment may represent an effective therapeutic alternative to prevent progression of chronic nephropathies.

Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

1986 ◽  
Vol 44 ◽  
pp. 342-343
Author(s):  
I. Stachura ◽  
M. Pardo ◽  
J. Costello ◽  
D.M. Landwehr
Keyword(s):  
Wistar Rats ◽  
Renal Damage ◽  
Standard Diet ◽  
Experimental Conditions ◽  
Phosphate Intake ◽  
Dietary Restrictions ◽  
Severe Reduction ◽  
Male Wistar Rats ◽  
Unrestricted Diet ◽  
Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Sign in / Sign up

Export Citation Format

Share Document