The original method for corpus callosum autopsy study in the case of diffuse axonal brain injury

In this work, we compared different DTI-based fibertracking software using deterministic and probabilistic approaches. DTI brain images of 35 healthy and five brain-injury patients were acquired with Philips Achieva 1.5 T scanner using an EPI-SE DTI sequence with 16 diffusion directions. Images were analyzed with Philips FiberTrack module, DTI-Studio and FSL. We studied corticospinal tract and corpus callosum, considering different termination criteria for the fibertracking algorithm. Group studies were performed to create a database of healthy patients. Results of FSL fibertracking with 1 or 2 fibers per voxel were no statistically different. T-tests between Philips and DTI-Studio led to p-values > 0.01 for corticospinal tract and < 0.01 for corpus callosum. FSL analysis led to higher ADC and lower FA values, with significative differences with the other software. In brain injury patients we measured different fibers orientation, reduced FA and increased ADC around the lesion. In conclusion, although DTI fibertracking is a promising non-invasive preoperative imaging tool, the outcome is strongly influenced by the algorithm used and the parameters chosen for the seed generation and fiber propagation.

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Corpus Callosum Vasculature Predicts White Matter Microstructure Abnormalities after Pediatric Mild Traumatic Brain Injury

Journal of Neurotrauma ◽

10.1089/neu.2018.5670 ◽

2019 ◽

Vol 36 (1) ◽

pp. 152-164 ◽

Cited By ~ 8

Author(s):

Kara M. Wendel ◽

Jeong Bin Lee ◽

Bethann M. Affeldt ◽

Mary Hamer ◽

Indira S. Harahap-Carrillo ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

White Matter ◽

Corpus Callosum ◽

Mild Traumatic Brain Injury ◽

White Matter Microstructure

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Abstract WP288: Inter-Alpha Inhibitor Proteins Reduce the Presence of Neutrophils and Matrix Metalloproteinase-9 Positive Neutrophils in Damaged Brain Regions of Neonates with Hypoxic-Ischemic (HI) Brain Injury

Stroke ◽

10.1161/str.48.suppl_1.wp288 ◽

2017 ◽

Vol 48 (suppl_1) ◽

Author(s):

Adriel Barrios-Anderson ◽

Xiaodi Chen ◽

Yow-Pin Lim ◽

Barbara S Stonestreet

Keyword(s):

Brain Injury ◽

Corpus Callosum ◽

Matrix Metalloproteinase ◽

Right Hemisphere ◽

Brain Regions ◽

Matrix Metalloproteinase 9 ◽

Male Rats ◽

Control Group ◽

Neonatal Rats ◽

Metalloproteinase 9

Introduction: Inter-alpha inhibitor proteins (IAIPs) are immunomodulatory proteins that play a significant anti-inflammatory role in hypoxic ischemic (HI) brain injury. We have shown that administering IAIPs after HI improves histopathological brain injury, brain weight, and behavioral outcomes in neonatal rats. Neutrophils are specialized leukocytes known to infiltrate the brain parenchyma and exacerbate neuronal injury after HI. One molecular mechanism by which neutrophils exert damage on the blood-brain barrier (BBB) and brain tissue after ischemia is by the release of matrix metalloproteinase-9 (MMP9), an enzyme that breaks down the extracellular matrices of surrounding cells. Objective: To determine the effect of IAIPs on neutrophil infiltration and release of MMP9 in neonatal rats after HI. Methods: The Vannucci model was used to induce neonatal HI in postnatal day 7 rats that were assigned to a Non-ischemic sham-control group (Sham, n=12), right-sided carotid ligation with exposure to hypoxia (8% oxygen for 90 min) treated with placebo group (PL-HI, n=17), or an IAIP treated group (IAIP-HI, n=17). Rat sex was recorded. IAIP (30 mg/kg) or PL was given intraperitoneally at 0, 24 and 48 h after HI. We removed the rat brain after 72h and performed immunohistochemistry using MPO (neutrophil selective) and MMP9 fluorescent markers. We performed stereological analyses with the StereoInvestigator 10.0 Fractionator probe without knowledge of group assignment to quantify neutrophils and MMP9 positive cells present within the right hemisphere, cortex, corpus callosum, and hippocampus. Results: MPO positive cells were significantly reduced in male IAIP treated rats compared with PL-HI in the overall damaged hemisphere (p<0.01) and the corpus callosum (p<0.05). Further, we observed MPO and MMP9 co-localization, and IAIP treatment reduced the presence of MMP9 positive neutrophils in the cortex of male rats compared to placebo (P<0.05).

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Genu of corpus callosum in diffuse axonal injury induces a worse 1-year outcome in patients with traumatic brain injury

Acta Neurochirurgica ◽

10.1007/s00701-011-1002-9 ◽

2011 ◽

Vol 153 (8) ◽

pp. 1687-1694 ◽

Cited By ~ 8

Author(s):

Hidetoshi Matsukawa ◽

Masaki Shinoda ◽

Motoharu Fujii ◽

Osamu Takahashi ◽

Daisuke Yamamoto ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Corpus Callosum ◽

Axonal Injury ◽

Diffuse Axonal Injury

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GERIATRIC TRAUMATIC BRAIN INJURY AND ALZHEIMER’S DISEASE SHARE SIMILAR PATTERNS OF WHITE MATTER DEGRADATION

Innovation in Aging ◽

10.1093/geroni/igz038.363 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

pp. S96-S96

Author(s):

Andrei Irimia ◽

Kenneth Rostowsky ◽

Nikhil Chaudhari ◽

Maria Calvillo ◽

Sean Lee

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Traumatic Brain Injury ◽

Brain Injury ◽

White Matter ◽

Corpus Callosum ◽

Diffusion Tensor ◽

Future Research ◽

Post Injury ◽

Significant Difference

Abstract Although mild traumatic brain injury (mTBI) and Alzheimer’s disease (AD) are associated with white matter (WM) degradation, the nature of these alterations and the outcomes of their comparison have not been elucidated. Diffusion tensor imaging (DTI) has been utilized in both conditions, and has uncovered decreases in the fractional anisotropy (FA) of the corpus callosum and cingulum bundle, compared to healthy control (HC) volunteers [1, 2]. Despite mTBI being a potential risk factor for AD, no systematic quantitative comparison has been drawn between their WM degradation patterns. Here we investigated WM FA differences using DTI and tract-based spatial statistics (TBSS) between age- and sex-matched adults: 33 chronic mTBI patients, 67 AD patients and 81 HC participants. T1-weighted magnetic resonance imaging (MRI) and DTI were acquired at 3T. mTBI patients were scanned acutely and ~6 months post-injury. FSL software was used for artefact correction, FA computation and TBSS implementation. Statistical comparison of WM FA patterns between mTBI and AD patients was achieved by two one-sided t tests (TOSTs) of statistical equivalence, with equivalence bounds defined where Cohen’s d < 0.3. A significant difference was found between the FA means of mTBI vs. HC groups, and the AD vs. HC groups (p < 0.01, corrected). Mean FA differences between mTBI and AD were statistically equivalent in the corpus callosum and in the inferior longitudinal fasciculus (p < 0.05, corrected). Future research should focus on clarifying the similarities between mTBI and AD, potentially leading to novel hypotheses and improved AD diagnosis.

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