scholarly journals Automated Liquid Handling for Microplate Assays: a Simplified User Interface for the Hamilton Microlab STAR

2021 ◽  
Vol 7 (1) ◽  
pp. 11-18
Author(s):  
Neville Ng ◽  
Reece Gately ◽  
Lezanne Ooi
Keyword(s):  
User Interface ◽  
Liquid Handling ◽  
High Throughput Drug Screening ◽  
Simple Program ◽  
Rapid Control ◽  
Liquid Transfer ◽  
Core Facilities ◽  
Program Interface ◽  
Drug Dilution ◽  
Complex Liquid

Automated liquid handling stations such as the Hamilton Microlab range can be implemented to greatly enhance throughput of cell-based and cell-free assays. To facilitate utilisation past the comprehensive programming interface of the Hamilton Method Editor this paper presents a user interface (UI) that runs within Hamilton Venus and allows for a user to control and store programs for plate-to-plate transfers and serial dilutions in 96 well plate format. The interface allows for rapid control of aspiration and dispensing height, trituration, control of tip rack selection, and includes a tip washing program that can reduce the number of disposable tips utilised. The simple program interface allows the Hamilton Microlab to be used as a readily deployable microtitre plate handler, particularly for applications such as aliquoting cells for seeding, diluting a number of plates in medium, or transferring drug dilution arrays to multiple plates. This paper also discusses various optimisations to increase accuracy and rate of low volume liquid transfer. While complex liquid handling tasks such as high-throughput drug screening requires established core facilities, standalone liquid handlers with simplified interfaces can be utilised for smaller-scale research applications and educational purposes.

Teaching Java™

2011 ◽  
pp. 185-201
Author(s):  
Henry H. Emurian
Keyword(s):  
User Interface ◽  
Graphical User Interface ◽  
Test Performance ◽  
Self Efficacy ◽  
Learning Processes ◽  
Instructional Materials ◽  
Classroom Observations ◽  
Simple Program ◽  
Hands On ◽  
Hands On Learning

Information systems students in a graduate section and an undergraduate section of an introductory Java graphical user interface course completed the following initial assignments to learn a simple program: (1) automated programmed instruction tutoring, (2) hands-on learning with a lecture, and (3) collaborative peer tutoring. Tests of knowledge transfer and software self-efficacy were administered before students began the first assignment and following completion of each one. The results showed progressive improvement in rule test performance and software self-efficacy across the several instructional events. Taken together, the results of these classroom observations extend the generality of previous work to an updated set of instructional materials and assignments, and that outcome shows the reliability of the learning processes with new groups of students. Students who are new to Java had the privilege of exposure to an initial repertoire of teaching tactics that are synergistic and cumulative.

scholarly journals Transportable open-source application program interface and user interface for generic humanoids: TOUGH

2020 ◽  
Vol 17 (3) ◽  
pp. 172988142092160
Author(s):  
Vinayak Jagtap ◽  
Shlok Agarwal ◽  
Ameya Wagh ◽  
Michael Gennert
Keyword(s):  
User Interface ◽  
Open Source ◽  
Humanoid Robots ◽  
Application Program Interface ◽  
Sensors And Actuators ◽  
Seamless Integration ◽  
Application Program ◽  
Diverse Field ◽  
High Level ◽  
Program Interface

Humanoid robotics is a complex and highly diverse field. Humanoid robots may have dozens of sensors and actuators that together realize complicated behaviors. Adding to the complexity is that each type of humanoid has unique application program interfaces, thus software written for one humanoid does not easily transport to others. This article introduces the transportable open-source application program interface and user interface for generic humanoids, a set of application program interfaces that simplifies the programming and operation of diverse humanoid robots. These application program interfaces allow for quick implementation of complex tasks and high-level controllers. Transportable open-source application program interface and user interface for generic humanoids has been developed for, and tested on, Boston Dynamics’ Atlas V5 and NASA’s Valkyrie R5 robots. It has proved successful for experiments on both robots in simulation and hardware, demonstrating the seamless integration of manipulation, perception, and task planning. To encourage the rapid adoption of transportable open-source application program interface and user interface for generic humanoids for education and research, the software is available as Docker images, which enable quick setup of multiuser simulation environments.

scholarly journals Design of Microplate-Compatible Illumination Panels for a Semiautomated Benchtop Pipetting System

2019 ◽  
Vol 24 (4) ◽  
pp. 399-407 ◽  
Author(s):  
Pierre Baillargeon ◽  
Kervin Coss-Flores ◽  
Fakhar Singhera ◽  
Justin Shumate ◽  
Hannah Williams ◽  
...  
Keyword(s):  
User Interface ◽  
Open Source ◽  
Real Time ◽  
Graphical User Interface ◽  
Laboratory Automation ◽  
Liquid Handling ◽  
Essential Tool ◽  
Implementation Techniques ◽  
Software And Hardware ◽  
Transfer Volumes

Microplates are an essential tool used in laboratories for storing research materials and performing assays. Many types of laboratory automation exist that greatly reduce the effort needed to utilize microplates; however, there are cases where the use of such automation is not feasible or practical. In these instances, researchers must work in an environment where liquid handling operations are performed manually with handheld pipetting devices. This type of work is tedious and error-prone as it relies on researchers to manually track a significant amount of metadata, including transfer volumes, plate barcodes, well contents, and well locations. To address this challenge, we have developed an open-source, semiautomated benchtop system that facilitates manual pipetting using visual indicators. This device streamlines the process of identifying the location of wells so that the researcher can perform manual transfers in a more efficient, reliable, and accurate manner. This system utilizes a graphical user interface that allows the user to load worklists and then issues commands to illuminate wells of interest, providing a visual indicator for users to follow in real time. The software and hardware tools utilized for development, along with the implementation techniques used to produce this system, are described within.

scholarly journals NextflowWorkbench: Reproducible and Reusable Workflows for Beginners and Experts

2016 ◽  
Author(s):  
Jason P. Kurs ◽  
Manuele Simi ◽  
Fabien Campagne
Keyword(s):  
User Interface ◽  
User Interfaces ◽  
Training Session ◽  
Rna Seq ◽  
Development Environment ◽  
Workflow Systems ◽  
Workflow System ◽  
Desktop Computers ◽  
Scripting Language ◽  
Core Facilities

ABSTRACTComputational workflows and pipelines are often created to automate series of processing steps. For instance, workflows enable one to standardize analysis for large projects or core facilities, but are also useful for individual biologists who need to perform repetitive data processing. Some workflow systems, designed for beginners, offer a graphical user interface and have been very popular with biologists. In practice, these tools are infrequently used by more experienced bioinformaticians, who may require more flexibility or performance than afforded by the user interfaces, and seem to prefer developing workflows with scripting or command line tools. Here, we present a workflow system, the NextflowWorkbench (NW), which was designed for both beginners and experts, and blends the distinction between user interface and scripting language. This system extends and reuses the popular Nextflow workflow description language and shares its advantages. In contrast to Nextflow, NextflowWorkbench offers an integrated development environment that helps complete beginners get started with workflow development. Auto-completion helps beginners who do not know the syntax of the Nextflow language. Reusable processes provide modular workflows. Programmers will benefit from unique interactive features that help users work more productively with docker containers. We illustrate this tool with a workflow to estimate RNA-Seq counts using Kallisto. We found that beginners can be taught how to assemble this workflow in a two hours training session. NW workflows are portable and can execute on laptop/desktop computers with docker, on a lab cluster, or in the cloud to facilitate training. NextflowWorkbench is open-source and available at http://workflow.campagnelab.org.

Application of Neural Network in Predicting Forging Hardness

2020 ◽  
Vol 305 ◽  
pp. 169-177
Author(s):  
Yuan Ping Luh ◽  
Chang Hung Tu ◽  
Huang Li Wang ◽  
Chien Jung Chiu
Keyword(s):  
Neural Network ◽  
Heat Treatment ◽  
User Interface ◽  
Graphical User Interface ◽  
Cold Forging ◽  
High Quality ◽  
Simple Program ◽  
Experimental Parameters ◽  
Neck Diameter ◽  
The Relationship

This study used cold forging to produce car components with the desired hardness without using postprocessing methods such as heat treatment, surface blasting, and polishing. Material diameter, mold neck diameter, and hardness of the material after annealing were used as parameters, and nine sets of experimental parameters were obtained using the Taguchi method. Under the premise of high-quality forging, this study determined the optimal hardness and the relationship between formation parameters and forging hardness. By inputting the hardness data obtained from the Taguchi L9 orthogonal array into a Matlab-implemented neural network, this study determined a hardness formula. Finally, using the inbuilt graphical user interface software of Matlab, a simple program was written that can be used to predict hardness and that could extensively reduce the costs and time associated with forging development.

scholarly journals Prototype implementation of segment assembling software

2018 ◽  
Vol 15 (1) ◽  
pp. 71-83
Author(s):  
Djordje Pesic ◽  
Marko Misic ◽  
Jelica Protic ◽  
Vujosevic Janicic
Keyword(s):  
User Interface ◽  
Graphical User Interface ◽  
Algorithmic Complexity ◽  
Prototype Implementation ◽  
Data Dependencies ◽  
Program Segment ◽  
Simple Program ◽  
It Education ◽  
Programming Knowledge ◽  
Examination Process

IT education is very important and a lot of effort is put into the development of tools for helping students to acquire programming knowledge and for helping teachers in automating the examination process. This paper describes a prototype of the program segment assembling software used in the context of making tests in the field of algorithmic complexity. The proposed new program segment assembling model uses rules and templates. A template is a simple program segment. A rule defines combining method and data dependencies if they exist. One example of program segment assembling by the proposed system is given. Graphical user interface is also described.

scholarly journals Visual Representation of Experimental Protocols

2017 ◽  
Author(s):  
James Scott-Brown ◽  
Antonis Papachristodoulou
Keyword(s):  
User Interface ◽  
Visual Representation ◽  
Cartesian Product ◽  
Liquid Handling ◽  
Experimental Protocols ◽  
The Creation ◽  
Readable Format

AbstractUsing robots to automate laboratory tasks could increase throughput and reproducibility, but requires experimental protocols to be specified in a computer-readable format. We present a new user interface (Lists of Liquids) for specifying experimental protocols by directly manipulating a diagram: rather than having to specify individual liquid handling operations, the user can simply specify that particular lists of liquids should be combined as either a Cartesian product or convolution, and the system will plan a series of liquid handling steps to achieve this. This is intended to provide a higher-level interface in order to make the creation of protocols faster and less error prone.

Remote On-Line Control of a High-Voltage in situ Transmission Electron Microscope with A Rational User Interface

1996 ◽  
Vol 54 ◽  
pp. 384-385
Author(s):  
M.A. O’Keefe ◽  
J. Taylor ◽  
D. Owen ◽  
B. Crowley ◽  
K.H. Westmacott ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
User Interface ◽  
Transmission Electron Microscope ◽  
High Voltage ◽  
Materials Science ◽  
Transmission Electron ◽  
On Line ◽  
Electron Microscopes

Remote on-line electron microscopy is rapidly becoming more available as improvements continue to be developed in the software and hardware of interfaces and networks. Scanning electron microscopes have been driven remotely across both wide and local area networks. Initial implementations with transmission electron microscopes have targeted unique facilities like an advanced analytical electron microscope, a biological 3-D IVEM and a HVEM capable of in situ materials science applications. As implementations of on-line transmission electron microscopy become more widespread, it is essential that suitable standards be developed and followed. Two such standards have been proposed for a high-level protocol language for on-line access, and we have proposed a rational graphical user interface. The user interface we present here is based on experience gained with a full-function materials science application providing users of the National Center for Electron Microscopy with remote on-line access to a 1.5MeV Kratos EM-1500 in situ high-voltage transmission electron microscope via existing wide area networks. We have developed and implemented, and are continuing to refine, a set of tools, protocols, and interfaces to run the Kratos EM-1500 on-line for collaborative research. Computer tools for capturing and manipulating real-time video signals are integrated into a standardized user interface that may be used for remote access to any transmission electron microscope equipped with a suitable control computer.

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