PLS calibration to resolve overlapping peaks of lutein and zeaxanthin in vegetable samples by LC

The chromatographic method in combination with a Partial Least Squares (type PLS-1) chemometric tool was developed to analyse simultaneously the carotenoid compounds, lutein and zeaxanthin. Both analytes appear together in the same chromatographic peak. Different calibration matrices were done using the absorbance spectra, obtained in the peak apex. The method was successfully applied to plant material such as cabbage, broccoli, red pepper, yellow pepper, and green pepper. The higher content of lutein was found in the green leafy vegetables such as broccoli and cabbage. On the other hand, zeaxanthin was only found in pepper, with the highest concentration having been found in red pepper.  

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Extraction of Light Filth from Canned Green Leafy Vegetables: Collaborative Study

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/62.3.600 ◽

1979 ◽

Vol 62 (3) ◽

pp. 600-601

Author(s):

Russell G Dent ◽

Jack L Boese

Keyword(s):

Plant Material ◽

Collaborative Study ◽

Excessive Amount ◽

Leafy Vegetables ◽

New Method ◽

Official Method ◽

Green Leafy Vegetables

Abstract The official first action method, 44.108, for the extraction of light filth from whole or chopped canned greens specifies a waterheptane flotation that results in the flotation of an excessive amount of interfering plant material and in low recoveries. A new method has been developed using a 40% isopropanol-watermineral oil flotation. The new method yielded a decreased amount of plant material and 84.9% recovery of the aphid spike compared with 42.5% recovery of the spike by the official method. The new method has been adopted as official first action to replace the existing method, except for broccoli.

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Prevalence of intestinal parasites in vegetables sold in major markets in Ibadan city, south-west Nigeria

Global Journal of Pure and Applied Sciences ◽

10.4314/gjpas.v21i1.2 ◽

1970 ◽

Vol 21 (1) ◽

pp. 7-12

Author(s):

A Adejumoke ◽

O Morenikeji

Keyword(s):

Intestinal Parasites ◽

Strongyloides Stercoralis ◽

Leafy Vegetables ◽

Plant Tissues ◽

Green Leafy Vegetables ◽

Vegetable Samples ◽

Sedimentation Technique ◽

South West ◽

High Prevalence ◽

The City

Studies were carried out in Ibadan City, South-West Nigeria between March and June 2011, to determine the prevalence of intestinal parasites in vegetables sold in various markets within the city. Fifty samples each of 5 different vegetable types, Cabbage (Brassica deracea), Lettuce (Lactus sativa), Carrot (Daucus carota), Cucumber and green leafy vegetables were bought randomly from five different markets and examined for parasites in the laboratory using sedimentation method. Results show that 29(11.6%) out of the 250 vegetable samples examined had intestinal parasites. The prevalence of intestinal parasites in these plant tissues was found to be 22.0% in Cabbage, 14.0% in Carrot, 12.0% in lettuce and 10.0% in Green leafy vegetables. No parasites were detected in the 50 cucumber samples. Intestinal parasites detected included ova of Ascaris lumbricoides (51.7%), Hookworm larvae (27.6%) and larvae of Strongyloides stercoralis (20.7%). This study shows a high prevalence of intestinal parasites on vegetables in Ibadan markets and the need for public enlightenment campaigns on the danger of consuming inadequately washed and raw vegetables.KEYWORDS: Intestinal parasites, vegetable, market, sedimentation technique, South-West Nigeria.

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Validation of a fast and accurate chromatographic method for detailed quantification of vitamin E in green leafy vegetables

Food Chemistry ◽

10.1016/j.foodchem.2013.03.099 ◽

2013 ◽

Vol 141 (2) ◽

pp. 1175-1180 ◽

Cited By ~ 17

Author(s):

Rebeca Cruz ◽

Susana Casal

Keyword(s):

Vitamin E ◽

Chromatographic Method ◽

Leafy Vegetables ◽

Green Leafy Vegetables

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Immunity boosting diet during Covid 19

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl1.3089 ◽

2020 ◽

Vol 11 (SPL1) ◽

pp. 832-838

Author(s):

Roshna Sukheoji Bhutada ◽

Renu Rathi ◽

Devyani Dasar

Keyword(s):

Immune System ◽

Food Studies ◽

Egg Yolk ◽

The Body ◽

Leafy Vegetables ◽

Green Leafy Vegetables ◽

Custard Apple ◽

The Right ◽

Daily Physical Activities ◽

Physiological Reactions

WHO declared Covid 19 /SARS -COV-2 as a global pandemic.Till date, there is no medicine for COVID-19. If the Infection arises in the body then the defence mechanism activated against infection. A recent study suggests that temporarily augmenting the body's immune system in the early stages of COVID-19 can help patient to avoid severe symptoms as it is rightly said prevention is better than cure. Ayurveda approaches to develop physiological reactions to facilitate immunity. Planning of diet is most important to boost immunity.As per many researches to provide supplementary food which contains Zinc, Vitamin C,Vitamin D and immunity boosting foodsuch as citrus natural products, custard apple, apple, papaya is among the Fruits. Vegetables include broccoli, onion, garlic and green leafy vegetables. Nuts, ginger, turmeric, pepper, egg yolk, shellfish, mushroom. The need of the hour is a quick boost to immune system to keep it fit, fighting. One should get the right amount of nutrients from the diet, supplementation regimen to boost immune system.In this review, there are few common supplements and super food studies have been included. It might be a torch bearer as sample menu and their alternatives are given for a normal adult. Needy may change contemplated according to age, sex, body mass index and daily physical activities.

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Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

Revista de Chimie ◽

10.37358/rc.19.6.7266 ◽

2019 ◽

Vol 70 (6) ◽

pp. 2015-2020

Author(s):

Silvia Robu ◽

Aurelia Romila ◽

Olimpia Dumitriu Buzia ◽

Adrian Florin Spac ◽

Camelia Diaconu ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Plant Material ◽

Mobile Phase ◽

Chromatographic Method ◽

Salvia Officinalis ◽

Salvia Officinalis L ◽

Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

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Do dietary patterns and morbidities have a relationship with primary infertility among women? A study from NFHS-4 (2015–16), India

Journal of Biosocial Science ◽

10.1017/s0021932021000274 ◽

2021 ◽

pp. 1-16

Author(s):

Sayeed Unisa ◽

Kanchan Negi ◽

Sucharita Pujari ◽

Vaishali Chaurasia

Keyword(s):

Dietary Patterns ◽

Family Health ◽

Severe Anaemia ◽

Married Women ◽

Leafy Vegetables ◽

Thyroid Disorder ◽

Daily Consumption ◽

High Blood Glucose ◽

Green Leafy Vegetables ◽

Primary Infertility

Abstract This study assessed the rate of primary infertility and its associated factors among 402,807 currently married women aged 20–49 years in India using National Family Health Survey-4 data collected in 2015–2016. Dietary patterns and selected morbidities were included as independent variables, and socioeconomic variables were considered as covariates. Bivariate and multivariate analyses were done to estimate the prevalence of primary infertility and assess its association with the selected variables, respectively. The rate of primary infertility among currently married women in India in 2015–16 was 1.9% and this was significantly associated with younger age (<35 years), higher age at marriage (≥18 years), urban residence, higher secondary or above education and poverty. The consumption of dairy products (OR = 0.79, CI = 0.73–0.86), dark green leafy vegetables (OR = 0.57, CI = 0.39–0.81) and fruit (OR = 0.88, CI = 0.77–1.01) significantly reduced the odds of primary infertility. Daily consumption of fish and aerated drinks was related to 1.06–1.21 times higher odds of primary infertility. Overweight/obesity, high blood pressure and high blood glucose levels were associated with 1.08–1.21 times elevated odds of primary infertility. Thyroid disorder (OR = 1.38, CI = 1.21–1.60), heart disease (OR = 1.17, CI = 1.16–1.19) and severe anaemia (OR = 1.24, CI = 1.00–1.53) were associated with an increased likelihood of primary infertility among women (OR 1.17–1.39, CI 1.00–1.60). The findings provide compelling evidence that primary infertility among women is related to dietary patterns and morbidities. Interventions and programmes targeting the promotion of healthy diets and lifestyles could be beneficial in addressing the issue of primary infertility among women.

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Biotransformation of Non-steroidal Anti-inflammatory Drugs Induces Ultrastructural Modifications in Green Leafy Vegetables

Journal of soil science and plant nutrition ◽

10.1007/s42729-021-00449-5 ◽

2021 ◽

Author(s):

Ocsana Opriș ◽

Maria-Loredana Soran ◽

Ildikó Lung ◽

Alexandra Ciorîță ◽

Lucian Copolovici

Keyword(s):

Leafy Vegetables ◽

Green Leafy Vegetables ◽

Inflammatory Drugs ◽

Ultrastructural Modifications ◽

Anti Inflammatory

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Identification of Plant Material by Its Phenolic Content

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/50.3.727 ◽

1967 ◽

Vol 50 (3) ◽

pp. 727-734

Author(s):

M Bonner Duggan

Keyword(s):

Thin Layer ◽

Plant Material ◽

Phenolic Content ◽

Pyrus Communis ◽

Flavonol Glycosides ◽

Ultraviolet Absorbance ◽

Flavonoid Compounds ◽

Phenolic Constituents ◽

Minor Compounds ◽

Absorbance Spectra

Abstract Methods have been developed to extract and chroniatograph phenolic constituents of Mains sylvestris and Pyrus communis fruits. Differences in the occurrence of flavonoid compounds between the two fruits and between pulp and peel in a given fruit were compared; the flavonol glycosides most conveniently demonstrated the differences. Five major and three minor flavonol glycosides from Stayman apples and two major and two minor ones from Packingham pears were separated by thin layer chromatograpliy. All of the major and some of the minor compounds were also described by ultraviolet absorbance spectra. Studies show that fruits can be distinguished on the basis of chromatographic patterns of the flavonol glycosides and that the results can be confirmed by their ultraviolet absorbance spectra

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Determinants and Suitability of Carotenoid Reflection Score as a Measure of Carotenoid Status

Nutrients ◽

10.3390/nu12010113 ◽

2020 ◽

Vol 12 (1) ◽

pp. 113 ◽

Cited By ~ 1

Author(s):

Elaine Rush ◽

Isaac Amoah ◽

Tung Diep ◽

Shabnam Jalili-Moghaddam

Keyword(s):

Body Size ◽

Health Outcomes ◽

Daily Intake ◽

Leafy Vegetables ◽

Carotenoid Concentration ◽

Dairy Foods ◽

Green Leafy Vegetables ◽

Positive Correlations ◽

Dark Green ◽

Negative Health Outcomes

Carotenoids, orange-coloured pigments found in vegetables, fruit, eggs and dairy foods, act as antioxidants and vitamin A precursors in the human body. Skin carotenoid concentration is a biomarker of vegetable and fruit intake. The aim was to identify determinants of skin carotenoid concentration by measuring “Veggie Meter™” carotenoid reflection spectroscopy scores (CRS) from the fingertip of adults with a range of ages, ethnicity and body size. Frequencies of daily intake of vegetables and fruit and weekly intake of pumpkin and carrot, dark green leafy vegetables (DGLV), eggs (yolk), and dairy were determined from a self-completed food-frequency-questionnaire. A total of 571 (324 Women, 247 Men) adults, aged 16 to 85 years, completed measurements. The CRS ranged from 83 to 769, with a median of 327. Women and men did not score differently. For all participants there were negative correlations of CRS with weight (r = −0.312) and BMI (r = −0.338) and positive correlations with weekly intakes of DGLV (r = 0.242) and carrots and pumpkin (r = 0.202). Based on a review of health outcomes associated with plasma carotenoids, 82% of the participants in the current study are at moderate risk, or more, of negative health outcomes. Determinants of carotenoid status were body size, intake of DGLV, carrots and pumpkin, and ethnicity.

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