Effects of diet supplementation with herbal blend and sunflower seeds on fermentation parameters, microbial population, and fatty acid profile in rumen of sheep

This study aimed to evaluate the effects of supplementation with sunflower seeds and vitamin E for fattening lambs, on the chemical composition, cholesterol, vitamin E level, fatty acid profile and lipid oxidation of meat from sheep. Four treatments were evaluated, as follows: SC – basal diet consisting of sugarcane + concentrate; SCS – basal diet supplemented with sunflower seed; SCE – basal diet supplemented with 1000 mg vitamin E; SCSE – basal diet supplemented with sunflower seed and 1000 mg vitamin E. The Longissimus lumborum muscle of 32 Ile de France lambs, which were slaughtered when their bodyweight reached 32 kg, was used. The chemical composition of the lamb meat was not significantly different (P > 0.05) between the treatments. Sunflower seed supplementation increased (P < 0.05) the levels of linoleic acid, vaccenic acid and conjugated linoleic acid. Inclusion of vitamin E in the diet increased (P < 0.05) the concentration of vitamin E in the meat while decreasing lipid oxidation. Considering the current demand for healthy foods, inclusion of sunflower seeds and vitamin E in the diet of sheep is a viable possibility.

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Profiling of in vitro rumen digestibility, fermentation parameters and fatty acid biohydrogenation of palm kernel cake-based diet supplemented with corn

10.21203/rs.3.rs-282753/v1 ◽

2021 ◽

Author(s):

Osama Anwer Saeed ◽

Umar M. Sani ◽

Awis Q. Sazili ◽

Henny Akit ◽

Abdul R. Alimon ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Microbial Population ◽

Unsaturated Fatty Acids ◽

Palm Kernel ◽

Gas Production ◽

Palm Kernel Cake ◽

Significant Difference ◽

Fermentation Parameters

Abstract Corn supplementation can enhance the function of rumen and mitigate methane production. Thus, this study aimed to evaluate in vitro rumen digestibility, fermentation parameters and fatty acid biohydrogenation of palm kernel cake-based (PKC) diet substituted with different levels of corn. Corn was substitution into PKC basal diet at the levels; T1= (0% corn + 75.3% PKC), T2= (5% corn + 70.3% PKC) and T3= (10% corn + 65.3% PKC) of the diet. Rumen liquor was obtained from four fistulated Dorper sheep and incubated with 200 mg of each treatment for 24hrs and 72hrs. Net gas production, fermentation kinetics, in vitro organic matter digestibility (IVOMD), in vitro dry matter digestibility (IVDMD), volatile fatty acids (VFA), rumen microbial population and fatty acid biohydrogenation were determined. The results of the in vitro study showed that production of gas increased from 0 hr until 9 hrs with T2 having the highest gas production during this phase. After 48 hrs, the gas production began to decrease gradually with increase in incubation time. No significant differences were observed in the IVDMD, IVOMD, NH3-N, pH and VFA at 72 hrs. However, higher significant methane gas (CH4) production was observed in T3 when compared with T1 and T2. Microbial population did not differ significantly between treatment groups for total bacteria, F. succinogenes and R. flavefaciens. The rates of biohydrogenation were not affected by corn substitution although a significant difference was observed in that of C18:1n9. In conclusion, corn substitution maintained fermentation characteristics with increasing of unsaturated fatty acids.

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In Vitro Ruminal Fermentation, Microbial Population, Fatty Acid Profile in Cattle in the Presence of Sapindus rarak Extract Combined With Oils Microencapsulation

Advances in Animal and Veterinary Sciences ◽

10.17582/journal.aavs/2019/7.9.798.805 ◽

2019 ◽

Vol 7 (9) ◽

Author(s):

Sri Suharti ◽

Isma Firliani ◽

Mawar Mawar ◽

Vishara Sekar ◽

Komang Gede Wiryawan

Keyword(s):

Fatty Acid ◽

Fatty Acid Profile ◽

Microbial Population ◽

Ruminal Fermentation

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Differential effect of maternal diet supplementation with α-Linolenic adcid or n-3 long-chain polyunsaturated fatty acids on glial cell phosphatidylethanolamine and phosphatidylserine fatty acid profile in neonate rat brains

Nutrition & Metabolism ◽

10.1186/1743-7075-7-2 ◽

2010 ◽

Vol 7 (1) ◽

pp. 2 ◽

Cited By ~ 14

Author(s):

Frédéric Destaillats ◽

Corinne Joffre ◽

Niyazi Acar ◽

Florent Joffre ◽

Jean-Baptiste Bezelgues ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Glial Cell ◽

Fatty Acid Profile ◽

Differential Effect ◽

Maternal Diet ◽

Neonate Rat ◽

Long Chain ◽

Diet Supplementation

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Methane emission, ruminal fermentation parameters and fatty acid profile of meat in Santa Inês lambs fed the legume macrotiloma

Animal Production Science ◽

10.1071/an19127 ◽

2020 ◽

Vol 60 (5) ◽

pp. 665 ◽

Cited By ~ 1

Author(s):

Paulo de Mello Tavares Lima ◽

Adibe Luiz Abdalla Filho ◽

Juliano Issakowicz ◽

Egon Hion Ieda ◽

Patrícia Spoto Corrêa ◽

...

Keyword(s):

Fatty Acid ◽

Fatty Acid Profile ◽

Relative Abundance ◽

Production System ◽

Carcass Characteristics ◽

Ch4 Emission ◽

Productive Performance ◽

Ruminal Fermentation ◽

Fermentation Parameters

Context Using legumes in ruminant production system may provide benefits such as improvement in animal performance and decrease in methane (CH4) emissions, mainly based on nutritional characteristics of these plants and the presence of tannins. Macrotiloma (Macrotyloma axillare) is a tropical legume that still lacks studies regarding its effects on animal performance and CH4 emissions. Aims The objective of this experiment was to evaluate productive performance, CH4 emission, ruminal fermentation parameters, carcass characteristics and fatty acid profile of the meat from lambs fed tropical grass hay supplemented with the legume forage macrotiloma. Methods For a 90-day experimental period, 14 Santa Inês male lambs (aging 100 days; 18.06 ± 3.26 kg initial bodyweight) were allocated in individual pens and divided into two treatments: chopped aruana grass (Panicum maximum cv. Aruana) hay (CON); aruana hay supplemented with chopped macrotiloma hay at a ratio of 75:25 grass to legume (MAC). Lambs were weighed fortnightly for calculation of average daily bodyweight gain (ADG) and CH4 emission was measured on three occasions (days 28 to 32; 57 to 61; 85 to 89) using the sulfur hexafluoride (SF6) tracer technique. At the last day of CH4 collection, rumen fluid samples were collected for: determination of ammoniacal nitrogen (NH3-N) and short chain fatty acids (SCFA); ruminal protozoa count; and relative abundance determination of general bacteria (BACT); Ruminococcus flavefaciens (RUMI); Fibrobacter succinogenes (FIBRO); methanogenic archaea (METH) by real-time quantitative PCR (real-time-qPCR) analysis. Subsequently, after 16 h fasting, the animals were slaughtered; carcass characteristics were then evaluated and longissimus thoracis samples were collected for fatty acid profile analysis. Key results No significant difference (P > 0.05) was observed between MAC and CON for productive performance, CH4 emission, SCFA and carcass characteristics. Reduced protozoa count and relative abundance of METH were observed for MAC (P < 0.05). Conclusions Despite the lack of effects on productive performance and CH4 emission, the decreased relative abundance of METH and protozoa count indicated that macrotiloma may possibly present anti-methanogenic activity. Implications The inclusion of this legume in the diet of ruminants may affect rumen microbes and potentially decrease environmental impacts of the production system.

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Effect of wastewater composition on microbial populations in biological phosphorus removal processes

Water Science & Technology ◽

10.2166/wst.1998.0039 ◽

1998 ◽

Vol 38 (1) ◽

pp. 159-166 ◽

Cited By ~ 4

Author(s):

J. C. Wang ◽

J. K. Park

Keyword(s):

Fatty Acid ◽

Fatty Acid Profile ◽

Phosphorus Removal ◽

Microbial Population ◽

Profile Analysis ◽

Cellular Fatty Acid ◽

Phosphorus Concentration ◽

Biological Phosphorus Removal ◽

Biological Phosphorus ◽

Cellular Fatty Acid Profile

Bench-scale sequential batch reactors (SBRs) were fed with glucose- and acetate-containing synthetic wastewaters to evaluate microbial population dynamics and types of phosphorus-accumulating organisms (PAOs) using a cellular fatty acid profile analysis. The phosphorus content in the sludge was 38% (w/w) for the acetate-fed SBR and 20% (w/w) for the glucose-fed SBR with a VSS/TSS ratio of 50%. Glucose-fed PAOs were found to remove phosphorus with accumulation of glycogen in cells without synthesizing poly-β-hydroxybutyrate (PHB) at influent phosphorus concentration < 20 mg-P/L and nitrate concentration < 2 mg-N/L. From the fatty acid profile biomarker study, it was found that the glucose-fed SBR maintained the same fatty acid profile before and after biological phosphorus removal (BPR) occurred while the acetate-fed SBR had a different fatty acid profile. The microbial population in the glucose-fed SBR was significantly different in terms of metabolic behavior and cellular fatty acid profile from that introduced in the acetate-fed SBR. Fatty acid a15:0 (anteiso methyl-branching) was abundant in the glucose-fed PAOs. Among the five PAO candidates (Acinetobacter, Pseudomonas, Arthrobacter, Aeromonas, and Micrococcus), only Arthrobacter spp. had the biomarker of fatty acid a15:0, indicating that Arthrobacter spp. may be one of the PAOs existing in the glucose-fed bioreactors.

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Effects of Saline and Sodic Stress on Yield and Fatty Acid Profile in Sunflower Seeds

Italian Journal of Agronomy ◽

10.4081/ija.2007.13 ◽

2007 ◽

Vol 2 (1) ◽

pp. 13 ◽

Cited By ~ 3

Author(s):

Giovanna Cucci ◽

Taddeo Rotunno ◽

Antonio De Caro ◽

Giovanni Lacolla ◽

Roberto Di Caterina ◽

...

Keyword(s):

Fatty Acid ◽

Fatty Acid Profile ◽

Sunflower Seeds

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The effect of diet supplementation with linseed scrap on the meat quality and fatty acid profile of the meat and backfat in fattening gilts

Veterinární Medicína ◽

10.17221/42/2019-vetmed ◽

2019 ◽

Vol 64 (No. 11) ◽

pp. 467-475

Author(s):

P Nevrkla ◽

E Vaclavkova

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Meat Quality ◽

Fatty Acid Profile ◽

Unsaturated Fatty Acids ◽

Profile Analysis ◽

Saturated Fatty Acids ◽

Total Content ◽

Control Group ◽

Diet Supplementation

The study was designed to evaluate the effect of diet supplementation with linseed on the carcass characteristics, meat quality and oxidative stability as well as the composition of the fatty acids in M. longissimus lumborum et thoracis (MLLT) and the backfat of fattening gilts. A total of 40 animals were used, 20 in the experimental and 20 in the control group. The results indicate that gilts fed with the control feed mixture (C) showed a higher (P < 0.05) content of intramuscular fat and backfat as compared to the experimentaL group (L) of gilts. A higher drip loss (P < 0.001) was recorded in the L group as same as higher pH<sub>45</sub> and pH<sub>24</sub> values (P < 0.01). The fatty acid profile analysis in the MLLT showed that the content of the MUFA (monounsaturated fatty acids) was lower (P < 0.01) in the L group than in the C group of the animals and also showed a higher (P < 0.01) content of the PUFA (polyunsaturated fatty acids) in the L group. The content of the n-6 and n-3 PUFA was higher (P < 0.001) in the L group. The ratio of the n-6/n-3 PUFA was significantly lower (P < 0.001) in the L group. The PUFA/SFA (saturated fatty acids) ratio was more favourable in the L group of gilts (P < 0.01). The results of the fatty acid profile analysis in the backfat proved the higher (P < 0.05) content of the UFA (unsaturated fatty acids) in the L group, while the content of the MUFA was lower (P < 0.001) in the L group. The total content of the SFA was lower (P < 0.05) in the L group. A higher content of the PUFA (P < 0.001) in the backfat was recorded in the L group than in the C group and the content of the n-3 PUFA was higher (P < 0.001) in the L group. The ratio of the n-6/n-3 PUFA was more favourable (P < 0.001) in the L group than in the C group. Also, the PUFA/SFA ratio was higher (P < 0.001) in the L group.

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