scholarly journals Optimisation of High Hydrostatic Pressure Assisted Extraction of Anthocyanins from Rabbiteye Blueberry Pomace

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 180-187 ◽  
Author(s):  
Zhang Haining ◽  
Ma Yongkun

The purpose of this study was to evaluate the influence of high hydrostatic pressure assisted extraction (HHPE) on the anthocyanins from blueberry (Vaccinium ashei) pomace. From the Plackett-Burman Experimental Design (PBD) only the liquid-solid ratio, ethanol concentration, and extraction pressure were found to significantly affect the extraction yield of anthocyanin content. Hence, the outcome of Box-Behnken Design suggested that the optimal operating conditions of the HHPE for the yield of anthocyanin content were liquid-solid ratio 41 ml/g, ethanol concentration 63%, and extraction pressure 443 MPa. At these conditions, 107.9 mg/100 g anthocyanins was obtained, which was more than by the control extraction (67.63 mg/100 g). 10 anthocyanins were identified by HPLC-ESI-MS, malvidin-3-galactoside and malvidin-3-glucoside were the major anthocyanins.

2013 ◽  
Vol 774-776 ◽  
pp. 919-922
Author(s):  
Xiao Song ◽  
Peng Zhao

Polysaccharide fromPanax japlcus var(PJVPs) was extracted with microwave-assisted (MAE). The effects of operating conditions, such as extraction time, ratio of water to raw material , and microwave power, on the extraction yield of PJVPs were studied through a Box-Behnken design (BBD). The optimum extraction conditions were as follows: extraction time 9.14 min, power 728.5 W and ratio of water to raw material 27.57ml/g. The yield of polysaccharide was 14.02 % based on the above mentioned conditions.


2015 ◽  
Vol 9 (1) ◽  
pp. 149-154
Author(s):  
Hu Zhiyong ◽  
Zhou Yajing ◽  
Xu Xia ◽  
Liu Shuyan ◽  
Jing Jie

Isoliquiritigenin (ILG) of licorice is a potential cancer chemopreventive agent. In this paper, response surface methodology (RSM) integrating with Box-Behnken design (BBD) was firstly employed to optimize the ILG extraction, which based on the single-factor experiments. According to analysis, the effect of extraction time and ethanol concentration on yields was significantly. The optimum values were the ethanol concentration of 73.25%, extraction time of 27.82 min and liquid-solid ratio of 19.10: 1. Under these conditions, the highest yield of ILG from licorice was 0.26 mg/g.


2012 ◽  
Vol 549 ◽  
pp. 504-507
Author(s):  
Xiao Rong Li ◽  
Peng Zhao

Polysaccharide from Acanthopanax obouatus Hoo(AHPs) was extracted with microwave-assisted(MAE). The effects of operating conditions, such as extraction time, ratio of water to raw material , and microwave power, on the extraction yield of AHPs were studied through a Box-Behnken design (BBD). The optimum extraction conditions were as follows: extraction time 13.5min, power 796 W and ratio of water to raw material 16.5ml/g. The yield of polysaccharide was 5.18% based on the above mentioned conditions.


2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Xue-gui Liu ◽  
Fu-yu Jiang ◽  
Pin-yi Gao ◽  
Mei Jin ◽  
Di Yang ◽  
...  

<p>The microwave-assisted extraction conditions of flavonoids in Physalis alkekengi. var. franchetii stems were optimized using a L33 Box-Behnken Design. The optimized extraction conditions were determined as follows: 60% for the ethanol concentration, 12.4 for the liquid-to-solid ratio, and 531.4 W for the microwave power, respectively. The yield of the extract obtained under the optimized conditions was 3.85 mg g−1 which was close to the predicted value. In addition, the extract exhibited potent acetylcholinesterase inhibitory activity, with the IC50 value of 11.61 μg mL−1 and a maximal inhibition ratio 89.81%. Both the yield and activity were better than reflux extraction.</p>


Author(s):  
Haining Zhang ◽  
William Tchabo ◽  
Yongkun Ma

The objective of this study was to compare the efficiency of high hydrostatic pressure, ultrasonic, microwave, and heat extraction techniques on the blueberry pomace extract characteristics. The results showed that the highest extraction yield of anthocyanins and total phenols were obtained by high hydrostatic pressure assisted extraction. The difference of extraction yield of anthocyanins was significant (p<0.05) between high hydrostatic pressure assisted extraction and heating extraction. Furthermore, significant difference of total phenols extraction yield was obtained between high hydrostatic pressure assisted extraction and the other three extraction methods. The total color difference was significant for the four extraction methods. High hydrostatic pressure assisted extraction had the highest chroma and least browning index. Moreover, high hydrostatic pressure assisted extraction was found to have the highest hydroxyl radical scavenging activity. In summary, high hydrostatic pressure assisted extraction was revealed to be the suitable technique for phenolic extraction of blueberry pomace.


2011 ◽  
Vol 138-139 ◽  
pp. 933-936 ◽  
Author(s):  
Xuan Chen ◽  
Hong Yu Luo ◽  
Jun Yu ◽  
Peng Xiang Yue ◽  
Lin Zhou ◽  
...  

Alcohol-alkali method and base digestion method were investigated to extract proteins from tea residues, respectively. According to single factorial experiments, results showed that the optimal extraction technology of alcohol-alkali method were pH 12, temperature of 80 °C, ethanol concentration of 60%, liquid-solid ratio of 40, 60 min, and the protein extraction rate reached 15.0%. And the optimal extract conditions of base digestion were pH 12, temperature of 80 °C, liquid-solid ratio of 50, 80 min, which made the protein yield reached 31.5%. Furthermore, alcohol-alkali method was more beneficial to protein extraction from tea residues under lower temperature and weak alkali condition (40-60 °C, pH 8-10). While base digestion had higher extraction yield under high temperature and strong alkali condition (60-80 °C, pH 11-12).


2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Claudia Giovagnoli-Vicuña ◽  
Sebastián Pizarro ◽  
Vilbett Briones-Labarca ◽  
Álvaro Delgadillo

Square wave voltammetry (SWV) analysis was used to assess the antioxidant interactions (synergism, addition, and antagonism) of fruit mixture extracts from grape (G), lemon (L), and blueberry (B) obtained by conventional extraction, ultrasound-assisted extraction, and high hydrostatic pressure extraction. The experimental results showed antagonistic antioxidant effects in all binary mixture extracts (L-G, L-B, and G-B). In DPPH and FRAP assays, the greatest antioxidant capacity was found in the G-B mixture (108.7 and 108.8 μmol TE g−1 dry extract, respectively) obtained by high hydrostatic pressure extraction; however, there were no significant differences when measured by ultrasound-assisted extraction. For TPC and TFC assays, the greatest values were for G-B (6.67 mg GA g−1 dry extract) and L-G (1.63 mg QE g−1 dry extract), respectively. SWV experiments showed antagonistic behavior in the mixtures. Among the different ratios of the fruit mixture extracts evaluated by SWV, 1 : 1 (w/w) combination showed the greatest antagonistic antioxidant effects. SWV suggests the components of the mixture with the highest antioxidant capacity oxidize after mixing. The results indicate that the presence of natural bioactive antioxidants in fruit mixtures does not guarantee that the interactions are synergistic.


Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1690
Author(s):  
Yohan Choi ◽  
Wooki Kim ◽  
Joo-Sung Lee ◽  
So Jung Youn ◽  
Hyungjae Lee ◽  
...  

Turmeric (Curcuma longa L.) is known for its health benefits. Several previous studies revealed that curcumin, the main active compound in turmeric, has antioxidant capacity. It has been previously demonstrated that puffing, the physical processing using high heat and pressure, of turmeric increases the antioxidant and anti-inflammatory activities by increasing phenolic compounds in the extract. The current study sought to determine if high hydrostatic pressure extraction (HHPE), a non-thermal extraction at over 100 MPa, aids in the chemical changes and antioxidant functioning of turmeric. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) analyses were conducted and assessed the content of total phenol compounds in the extract. The chemical changes of curcuminoids were also determined by high performance liquid chromatography (HPLC). Among the three variables of ethanol concentration, pressure level, and treatment time, ethanol concentration was the most influential factor for the HHPE of turmeric. HHPE at 400 MPa for 20 min with 70% EtOH was the optimal extraction condition for the highest antioxidant activity. Compositional analysis revealed that 2-methoxy-4-vinylphenol was produced by puffing. Vanillic acid and ferulic acid content increased with increasing HHPE time. Synergistic effect was not observed on antioxidant activity when the turmeric was sequentially processed using puffing and HHPE.


2017 ◽  
Vol 890 ◽  
pp. 167-170 ◽  
Author(s):  
Jessinta Sandanasamy ◽  
Mashitah M. Yusoff ◽  
Jolius Gimbun

This work presents an optimization study of polyphenol extraction from L. pumila with aid of sonication. The polyphenol content was evaluated using Singleton’s method and aluminium chloride colorimetric assay. The two level factorial design [2LF] was used to screen the significance of the extraction parameters. The results proposed that the polyphenols extraction are mainly affected by the total solid ratio [TSR] and residence time. The highest flavonoid content of 31.79 mg QE/ g DW and phenolic content of 19.65 mg GA/g DW was obtained from 80% and 10% aqueous ethanol [EtOH], respectively via 2LF design. Three most important parameters [TSR, residence time and amplitude] were then further optimized thru central composite design. The highest simultaneous flavonoid [21.77 mg QE/ g DW] and phenolic [21.86 mg GA/g DW] extraction yield were attained from the probe sonicator extraction with desirability of 0.998 at 10% aqueous EtOH with TSR 0.01, residence time of 13 min and amplitude of 55%.


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