scholarly journals Quality of extracts from blueberry pomace by high hydrostatic pressure, ultrasonic, microwave and heating extraction：A comparison study

2017 ◽  
pp. 815 ◽  
Author(s):  
Haining Zhang ◽  
William Tchabo ◽  
Yongkun Ma
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Heat Extraction ◽  
Total Phenols ◽  
Assisted Extraction ◽  
Significant Difference

The objective of this study was to compare the efficiency of high hydrostatic pressure, ultrasonic, microwave, and heat extraction techniques on the blueberry pomace extract characteristics. The results showed that the highest extraction yield of anthocyanins and total phenols were obtained by high hydrostatic pressure assisted extraction. The difference of extraction yield of anthocyanins was significant (p<0.05) between high hydrostatic pressure assisted extraction and heating extraction. Furthermore, significant difference of total phenols extraction yield was obtained between high hydrostatic pressure assisted extraction and the other three extraction methods. The total color difference was significant for the four extraction methods. High hydrostatic pressure assisted extraction had the highest chroma and least browning index. Moreover, high hydrostatic pressure assisted extraction was found to have the highest hydroxyl radical scavenging activity. In summary, high hydrostatic pressure assisted extraction was revealed to be the suitable technique for phenolic extraction of blueberry pomace.

scholarly journals Achievement of antioxidants from Abelmoschus esculentus L. seeds using ultrasound-assisted extraction optimized by the help of experimental design approach

2020 ◽  
Vol 14 (2) ◽  
pp. 160-168
Author(s):  
Merve Bat Özmatara ◽  
Şule Dinç Zor
Keyword(s):  
Experimental Design ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Extraction Time ◽  
Abelmoschus Esculentus ◽  
Scavenging Activity ◽  
Ultrasound Assisted Extraction ◽  
Extraction Solvent ◽  
Assisted Extraction

Developments of food supplements and nutraceuticals from different natural origins require the use of some extraction methods. The success of the procedure relies on theefficiency of the extraction methods. Therefore, extraction methods need to be optimized. This work aimed for the determination of optimal conditions of ultrasonic-assisted extraction (UAE) of antioxidant constituents from okra (Abelmoschus esculentus L.) seeds which contain high contents of polysaccharides, polyphenols and flavonoids using response-surface methodology based on Box-Behnken experimental design (BBD). The independent variables of UAE were extraction time (Et, 10-20 min), extraction solvent composition (EC, 50-100% methanol) and extraction solvent volume (EV, 10-20 mL). 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, 2,2'-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity and N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical-scavenging activity were dependent variables. Optimal extraction conditions for UAE from okra seed were: 20 mL volume of 70% methanol at 10 min extraction time for DPPH inhibition; 20 mL volume of 50% methanol at 20 min for ABTS inhibition; 15 mL volume of 55% methanol at 12 min for DMPD inhibition. Under these optimum extraction conditions, the effectiveness of UAE of okra seed was successfully revealed. So, this practical extraction technique can be widely used for the achievement of dietary supplements and nutraceuticals.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

2019 ◽  
Vol 92 (3) ◽  
pp. 369-377
Author(s):  
Barbara Fumić ◽  
Mario Jug ◽  
Marijana Zovko Končić
Keyword(s):  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Lotus Corniculatus ◽  
Extraction Yield ◽  
Phenolic Antioxidants ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Ultrasound-assisted extraction of phenolic antioxidants from Lotus corniculatus was optimized using response surface methodology. The extraction was performed according to the Box–Behnken design with ethanol concentration, temperature, and pH, as independent variables. The responses were extraction yield, DPPH radical scavenging activity (RSA) IC50 and content of different phenolic compounds (total phenols, flavonoids and phenolic acids, as well as quercetin, kaempferol and genistein derivatives). The models were used to calculate best conditions for maximal extraction of phenolic compounds and antiradical activity. Use of the optimized extraction parameters increased the content of quercetin and kaempferol derivatives more than tenfold (from 6.07 to 65.10 mg mL–1 and 6.69 to 92.75 mg mL–1, respectively). The results of this work stress the importance of careful selection of conditions for flavonoids extraction. Abundance of bioactive phenolics in L. corniculatus extracts obtained under optimized extraction conditions opens the possibility for wider utilization of this plant.

scholarly journals Optimisation of High Hydrostatic Pressure Assisted Extraction of Anthocyanins from Rabbiteye Blueberry Pomace

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 180-187 ◽  
Author(s):  
Zhang Haining ◽  
Ma Yongkun
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Anthocyanin Content ◽  
Vaccinium Ashei ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Assisted Extraction

The purpose of this study was to evaluate the influence of high hydrostatic pressure assisted extraction (HHPE) on the anthocyanins from blueberry (Vaccinium ashei) pomace. From the Plackett-Burman Experimental Design (PBD) only the liquid-solid ratio, ethanol concentration, and extraction pressure were found to significantly affect the extraction yield of anthocyanin content. Hence, the outcome of Box-Behnken Design suggested that the optimal operating conditions of the HHPE for the yield of anthocyanin content were liquid-solid ratio 41 ml/g, ethanol concentration 63%, and extraction pressure 443 MPa. At these conditions, 107.9 mg/100 g anthocyanins was obtained, which was more than by the control extraction (67.63 mg/100 g). 10 anthocyanins were identified by HPLC-ESI-MS, malvidin-3-galactoside and malvidin-3-glucoside were the major anthocyanins.

Combined effect of thermosonication and high hydrostatic pressure on bioactive compounds, microbial load, and enzyme activities of blueberry juice

2021 ◽  
pp. 108201322110043
Author(s):  
Tianshun Chen ◽  
Bin Li ◽  
Chi Shu ◽  
Jinlong Tian ◽  
Ye Zhang ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Hydrostatic Pressure ◽  
Enzyme Activities ◽  
High Hydrostatic Pressure ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Combined Effect ◽  
Soluble Solids ◽  
Microbial Load ◽  
Blueberry Juice

The current study is aimed to evaluate the combined effect of thermosonication (TS) and high hydrostatic pressure (HHP) on enzyme activities (polyphenolase and peroxidase), microbial load and phenolic compounds (phenols, flavonoids, and anthocyanins) of blueberry juice. Blueberry juice has been treated with TS (40 kHz and 240 W) at different temperatures (25 °C and 45 °C) for 15 mins with subsequent different HHP (200, 400 and 600 MPa) for 5 mins at room temperature. The results revealed that a combined use of HHP of 400 MPa and 600 MPa with TS at 45 °C not only reduced microorganisms below 1 logCFU/mL, but also significantly inactivated enzymes. The treatments also increased the phenolic compounds, peroxyl radical scavenging capacity (PSC), and DPPH free radical scavenging activity to a higher level without causing any changes in soluble solids and pH. Therefore, the combination of HHP and TS can be used as a novel alternative nonthermal technology to improve the nutritional qualities of blueberry juice, which produces a desirable, healthy juice for consumers.

scholarly journals Effect of extraction method on the antioxidative activity of ground elder (Aegopodium podagraria L.)

2019 ◽  
Vol 21 (3) ◽  
pp. 13-18 ◽  
Author(s):  
Agnieszka Wróblewska ◽  
Katarzyna Janda ◽  
Edyta Makuch ◽  
Marika Walasek ◽  
Piotr Miądlicki ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Extraction Time ◽  
Lower Amount ◽  
Antioxidative Properties ◽  
Assisted Extraction ◽  
Soxhlet Apparatus ◽  
Ultrasonic Assisted ◽  
Method Of Extraction

Abstract In this work the studies on the antioxidative properties of extracts from various morphotic parts of the ground elder (leaves, rhizomes, seeds and flowers) were presented. Moreover, the effect of different extraction methods (ultrasonic assisted extractions, extraction in a Soxhlet apparatus, extraction at the boiling point of the solvent used), solvent and its amount, and extraction time on the antioxidative properties of the obtained extracts were tested. The studies showed that all parts of ground elder can show radical scavenging activity, and it depends mainly on the method of extraction and extraction time. But the most beneficial is ultrasonic assisted extraction which used lower amount of solvent (ethanol). In case of all parts of the ground elder (leaves, rhizomes, seeds and flowers) it allows to obtain very high values of the antioxidant capacity (above 90%) for very short extraction time amounted to 20–40 minutes.

scholarly journals Comparison of Classical and Ultrasound-Assisted Extraction Methods on Antioxidant Activities of Pharmaceutically Active Compounds from Valeriana Officinalis

2021 ◽  
Vol 5 (2) ◽  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Valeriana Officinalis ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Dpph Radical Scavenging

Medicinal plants are good sources and rich of substances which having nutraceutical and health benefits. Valeriana officinalis L. (Valerianaceae) is a known medicinal plant used in traditional medicine worldwide. The aim of present study was to evaluate the efficiencies of three methods for evaluated for antioxidant from Valeriana officinalis. Besides, the impact of extraction methods on total phenollic and flavonoids contents and antioxidant activities of Valeriana root was investigated. The dried-root was extracted by three different methods including maceration, ultrasonic assisted, and Soxhlet assisted extraction. Antioxidant capacity was assessed using four different methods: DPPH and nitric oxide (NO) free radicals scavenging, reducing power and iron chelating activity. The total phenolic and flavonoid contents were also identified. The ultrasonic extract showed highest amount of total phenolics and flavonoids contents. In DPPH radical scavenging activity, the ultrasonic assisted extract, (IC50=0.546 μg/ ml) had a higher activity from other extracts. In reducing power assay, maceration extract showed the highest activity. In DPPH radical scavenging activity, IC50 for ultrasonic extract, soxhlet assisted extraction and maceration extract were 0.546, 0.816 and 0.678 μg/ml, respectively. Here, the maceratin extract showed the highest activity, too. The results clearly indicated the extraction methods used in this study significantly affected antioxidant capacities and total phenolic and flavonoids contents. Ultrasonic assisted extraction and Soxhlet methods were found to be more efficient in extraction of antioxidant components of valeriana.

Extraction and Quantification of Eugenol from Clove Buds Using HPLC

2020 ◽  
Vol 7 (1) ◽  
pp. 17-23
Author(s):  
Khadiza Fitri Shafira ◽  
Abul Kalam Azad ◽  
Zubair Khalid Labu ◽  
Abul Bashar Mohammed Helal Uddin
Keyword(s):  
Processing Time ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Past Study ◽  
Solvent Ratio ◽  
Clove Oil ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Significant Difference ◽  
Clove Essential Oil

Background: Eugenol is the main constituent of clove essential oil. Past studies have found that clove oil has diverse uses in the pharmaceutical field due to its antioxidant, antibacterial and anesthetic properties. Objective: This work compares the performance of different extraction methods and factors and identifies the effect of the treatments on oil yields and eugenol content. Materials and Methods: Maceration, Hydro distillation, microwave-assisted extraction (MAE), and Soxhlet were performed. The best technique was identified according to yield and content. Further studies were conducted to examine the effects of different factors, such as solvent types (ethanol and methanol) and sample-to-solvent ratio (1:10 and 1:15). HPLC UV-Vis was utilized in the analysis of eugenol concentration. Results and Discussion: Soxhlet extraction provided the highest yield (39.98%) and eugenol content (15.83%), compared to other methods. The results observed from several Soxhlet extraction factors showed that there is no significant difference between the different factors. In the meantime, methanol 1:15 provided the greatest amount of yields (57.83%) and eugenol content (22.21%). In this regard, the higher ratio resulted in higher eugenol content. Conclusion: The results obtained are less comparable because the processing time, the working solvent, and the separation technique were carried out differently for each method. In the meantime, as there is no past study that compared the selected methods and factors, this study’s findings will contribute substantially to fill the gap in this field.

scholarly journals Effect of Extraction Conditions on the Antioxidant Activity of Olive Wood Extracts

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Mercedes Pérez-Bonilla ◽  
Sofía Salido ◽  
Adolfo Sánchez ◽  
Teris A. van Beek ◽  
Joaquín Altarejos
Keyword(s):  
Geographical Origin ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Olive Tree ◽  
Extraction Yield ◽  
Scavenging Activity ◽  
Direct Extraction ◽  
Ethanolic Extracts ◽  
Wood Extracts ◽  
Main Components

An investigation to optimize the extraction yield and the radical scavenging activity from the agricultural by-product olive tree wood (Olea europaeaL., cultivar Picual) using six different extraction protocols was carried out. Four olive wood samples from different geographical origin, and harvesting time have been used for comparison purposes. Among the fifty olive wood extracts obtained in this study, the most active ones were those prepared with ethyl acetate, either through direct extraction or by successive liquid-liquid partitioning procedures, the main components being the secoiridoids oleuropein and ligustroside. An acid hydrolysis pretreatment of olive wood samples before extractions did not improve the results. In the course of this study, two compounds were isolated from the ethanolic extracts of olive wood collected during the olives’ harvesting season and identified as (7′′R)-7′′-ethoxyoleuropein (1) and (7′′S)-7′′-ethoxyoleuropein (2).

scholarly journals Biochemical Characterization of Clinical Strains of Staphylococcus spp. and Their Sensitivity to Polyphenols-Rich Extracts from Pistachio (Pistacia vera L.)

Pathogens ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 82 ◽  
Author(s):  
Erminia La Camera ◽  
Carlo Bisignano ◽  
Giuseppe Crisafi ◽  
Antonella Smeriglio ◽  
Marcella Denaro ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Clinical Isolates ◽  
Total Phenols ◽  
Clinical Strains ◽  
Novel Treatments ◽  
Ic50 Values ◽  
Staphylococcus Spp

We characterized a number of clinical strains of Staphylococcus spp. and investigated their sensitivity against polyphenols-rich extracts from natural raw and roasted pistachios (NPRE and RPRE, respectively). Out of 31 clinical isolates of Staphylococcus spp., 23 were coagulase-positive and identified as S. aureus, of which 21 were MRSA. Polyphenols-rich extracts from natural pistachios and roasted pistachios were prepared: the total phenols content, expressed as gallic acid equivalent (GAE)/100 g fresh weight (FW), was higher in natural pistachios (359.04 ± 8.124 mg) than roasted pistachios (225.18 ± 5.055 mg). The higher total phenols content in natural pistachios also correlated to the higher free-radical scavenging activity found by DPPH assay: NPRE and RPRE showed IC50 values of 0.85 (C.L. 0.725–0.976 mg mL−1) and 1.15 (C.L. 0.920–1.275 mg mL−1), respectively. Both NPRE and RPRE were active against S. aureus 6538P and Staph. spp. clinical isolates, with RPRE being the most active (MIC values ranging between 31.25 and 2000 μg mL−1). The antimicrobial potential of pistachios could be used to identify novel treatments for S. aureus skin infections.

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