scholarly journals Optimization of Extraction Conditions for Flavonoids of Physalis alkekengi var. franchetii Stems by Response Surface Methodology and Inhibition of Acetylcholinesterase Activity

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Xue-gui Liu ◽  
Fu-yu Jiang ◽  
Pin-yi Gao ◽  
Mei Jin ◽  
Di Yang ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Acetylcholinesterase Activity ◽  
Maximal Inhibition ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ic50 Value ◽  
Optimized Conditions ◽  
Better Than

<p>The microwave-assisted extraction conditions of flavonoids in Physalis alkekengi. var. franchetii stems were optimized using a L33 Box-Behnken Design. The optimized extraction conditions were determined as follows: 60% for the ethanol concentration, 12.4 for the liquid-to-solid ratio, and 531.4 W for the microwave power, respectively. The yield of the extract obtained under the optimized conditions was 3.85 mg g−1 which was close to the predicted value. In addition, the extract exhibited potent acetylcholinesterase inhibitory activity, with the IC50 value of 11.61 μg mL−1 and a maximal inhibition ratio 89.81%. Both the yield and activity were better than reflux extraction.</p>

scholarly journals Optimisation of High Hydrostatic Pressure Assisted Extraction of Anthocyanins from Rabbiteye Blueberry Pomace

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 180-187 ◽  
Author(s):  
Zhang Haining ◽  
Ma Yongkun
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Anthocyanin Content ◽  
Vaccinium Ashei ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Assisted Extraction

The purpose of this study was to evaluate the influence of high hydrostatic pressure assisted extraction (HHPE) on the anthocyanins from blueberry (Vaccinium ashei) pomace. From the Plackett-Burman Experimental Design (PBD) only the liquid-solid ratio, ethanol concentration, and extraction pressure were found to significantly affect the extraction yield of anthocyanin content. Hence, the outcome of Box-Behnken Design suggested that the optimal operating conditions of the HHPE for the yield of anthocyanin content were liquid-solid ratio 41 ml/g, ethanol concentration 63%, and extraction pressure 443 MPa. At these conditions, 107.9 mg/100 g anthocyanins was obtained, which was more than by the control extraction (67.63 mg/100 g). 10 anthocyanins were identified by HPLC-ESI-MS, malvidin-3-galactoside and malvidin-3-glucoside were the major anthocyanins.

scholarly journals PENGARUH WAKTU EKSTRAKSI DAN KONSENTRASI ETANOL MENGGUNAKAN METODE MICROWAVE ASSISTED EXTRACTION (MAE) TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK RAMBUT JAGUNG (Zea mays L.)

2019 ◽  
Vol 8 (1) ◽  
pp. 94
Author(s):  
Yessica Kristanti ◽  
I Wayan Rai Widarta ◽  
I Dewa Gede Mayun Permana
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Corn Silk ◽  
Assisted Extraction ◽  
Ic50 Value ◽  
Two Factors ◽  
Determine Effect

This research was conducted to determine effect of the extraction time and ethanol concentration on antioxidant activity of corn silk extract and to obtain extraction time and ethanol concentration using Microwave Assisted Extraction (MAE) that can produce extract with the highest antioxidant activity. The experimental design used in this research was a completely randomize design, which consisted of two factors. The fisrt factor was the concentration of ethanol which consisted of 5 level that namely ethanol 30%, 45%, 60%, 75%, and 90%. The second factor was the time of extraction which consisted of 3 level namely 8, 13 and 18 minutes. Data were analyzed with analysis of variance that followed by Duncan test. The result showed that the best treatment of corn silk extract is extraction by ethanol 90% for 18 minutes using MAE which the highest resulted rendemen was 24.54%, total phenol was 159.65 mg GAE/g extract, total flavonoid was 149 mgQE/g extract, antioxidant activity was 70.18%, and the IC50 value 64.806 ppm.

scholarly journals Optimization of Ultrasound Assisted Extraction of Isoliquiritigenin from Licorice by Response Surface Methodology

2015 ◽  
Vol 9 (1) ◽  
pp. 149-154
Author(s):  
Hu Zhiyong ◽  
Zhou Yajing ◽  
Xu Xia ◽  
Liu Shuyan ◽  
Jing Jie
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Chemopreventive Agent ◽  
Assisted Extraction ◽  
Potential Cancer

Isoliquiritigenin (ILG) of licorice is a potential cancer chemopreventive agent. In this paper, response surface methodology (RSM) integrating with Box-Behnken design (BBD) was firstly employed to optimize the ILG extraction, which based on the single-factor experiments. According to analysis, the effect of extraction time and ethanol concentration on yields was significantly. The optimum values were the ethanol concentration of 73.25%, extraction time of 27.82 min and liquid-solid ratio of 19.10: 1. Under these conditions, the highest yield of ILG from licorice was 0.26 mg/g.

scholarly journals Separation of the main flavonoids and essential oil from seabuckthorn leaves by ultrasonic/microwave-assisted simultaneous distillation extraction

2018 ◽  
Vol 5 (7) ◽  
pp. 180133 ◽  
Author(s):  
Chunying Li ◽  
Jingjing Zhang ◽  
Chunjian Zhao ◽  
Lei Yang ◽  
Wenyan Zhao ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Simultaneous Distillation Extraction ◽  
Ultrasonic Assisted Extraction ◽  
Optimized Conditions

Volatile essential oils (EOs), non-volatile rutin (RU), quercetin (QU), kaempferol (KA) and isorhamnetin (IS) were effectively extracted and isolated from seabuckthorn ( Hippophae rhamnoides L.) leaves by ionic liquid-based ultrasound/microwave-assisted simultaneous distillation extraction (ILUMASDE). After optimization by response surface methodology, EOs, RU, QU, KA and IS were separated under the following optimum conditions: an ionic liquid of 1.0 M 1-butyl-3-methyl imidazole bromine salt ([C4mim]), liquid/solid ratio of 12 ml g −1 , extraction time of 34 min, microwave power of 540 W and a fixed ultrasonic power of 50 W. Under the optimized conditions of ILUMASDE, the extraction yields of RU, QU, KA, IS and EOs were 9.18 ± 0.35, 5.52 ± 0.23, 3.03 ± 0.11, 5.64 ± 0.24 mg g −1 and 0.095 ± 0.004%, respectively. The yield of EOs obtained using ILUMASDE was 1.07-fold higher than that obtained by conventional hydrodistillation extraction (HDE). In addition, the components of the EOs obtained using ILUMASDE and HDE were similar. The extraction yields of RU, QU, KA, IS obtained by ILUMASDE were 1.03–1.35-fold higher than that obtained by the ethanol ultrasonic-assisted extraction (EUAE), ionic liquid-based ultrasonic-assisted extraction (ILUAE) and ionic liquid-based microwave-assisted extraction (ILMAE). And the extraction time used by ILUMASDE was 34 min, which is 14.17%, 56.67%, 56.67% and 85.00% less than those used by HDE, EUAE, ILUAE and ILMAE, respectively. Therefore, ILUMASDE can be considered a rapid and efficient method for extracting flavonoids and EO from seabuckthorn ( Hippophae rhamnoids L.) leaves.

scholarly journals Microwave-assisted Extraction of Spruce Bark: Statistical Optimization Using Box-Behnken Design

BioResources ◽  
2018 ◽  
Vol 13 (4) ◽  
Author(s):  
Alexandra Sládková ◽  
Ján Stopka ◽  
Aleš Ház ◽  
Petra Strižincová ◽  
Igor Šurina ◽  
...  
Keyword(s):  
Statistical Optimization ◽  
Microwave Assisted Extraction ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Spruce Bark ◽  
Assisted Extraction

scholarly journals Microwave-assisted extraction of non-polar compounds from pistachio hull and characterization of extracts

2018 ◽  
Vol 69 (3) ◽  
pp. 260 ◽  
Author(s):  
H. N. Özbek ◽  
D. Koçak Yanık ◽  
S. Fadıloğlu ◽  
H. Keskin Çavdar ◽  
F. Göğüş
Keyword(s):  
Microwave Power ◽  
Polar Compounds ◽  
Good Alternative ◽  
Tocopherol Content ◽  
Extraction Time ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction

Soxhlet and microwave assisted extraction (MAE) methods were used to obtain non-polar compounds from pistachio hull. MAE parameters (liquid to solid ratio, microwave power, and extraction time) were studied to obtain maximum extraction yield. The optimal conditions were found to be liquid to solid ratio of 15:1 (v/w), microwave power of 250 W and extraction time of 12.5 min. The extraction yields were 9.81 and 9.50% for MAE and Soxhlet methods, respectively. The total phenolic content, antioxidant activity and tocopherol content of the extract obtained by MAE was found to be significantly higher than those of the Soxhlet extract (p < 0.05). The results showed that the extract contained α-tocopherols (567.65 mg/kg) and oleic acid (48.46%) as the major tocopherols and fatty acids. These findings propose that hull extracts can be considered as a good source of natural bioactive compounds and MAE can be a good alternative to the traditional Soxhlet method.

scholarly journals Microwave-Assisted Extraction of Phlorotannins from Fucus vesiculosus

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 559
Author(s):  
Sónia J. Amarante ◽  
Marcelo D. Catarino ◽  
Catarina Marçal ◽  
Artur M. S. Silva ◽  
Rita Ferreira ◽  
...  
Keyword(s):  
Xanthine Oxidase ◽  
Conventional Method ◽  
Ethanol Concentration ◽  
Fucus Vesiculosus ◽  
Metabolic Enzyme ◽  
Microwave Assisted Extraction ◽  
Pharmaceutical Drug ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Conventional Extraction

Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from Fucus vesiculosus using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (v/v), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DWextract), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS•+ (IC50 of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC50 of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.

scholarly journals Effects of Microwave-Assisted Extraction Conditions on Antioxidant Capacity of Sweet Tea (Lithocarpus polystachyus Rehd.)

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 678
Author(s):  
Ao Shang ◽  
Min Luo ◽  
Ren-You Gan ◽  
Xiao-Yu Xu ◽  
Yu Xia ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Optimal Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Sample Ratio

In this study, the effects of microwave-assisted extraction conditions on antioxidant capacity of sweet tea (Lithocarpus polystachyus Rehd.) were studied and the antioxidants in the extract were identified. The influences of ethanol concentration, solvent-to-sample ratio, microwave power, extraction temperature and extraction time on Trolox equivalent antioxidant capacity (TEAC) value, ferric reducing antioxidant power (FRAP) value and total phenolic content (TPC) were investigated by single-factor experiments. The response surface methodology (RSM) was used to study the interaction of three parameters which had significant influences on antioxidant capacity including ethanol concentration, solvent-to-sample ratio and extraction time. The optimal conditions for the extraction of antioxidants from sweet tea were found as follows—ethanol concentration of 58.43% (v/v), solvent-to-sample ratio of 35.39:1 mL/g, extraction time of 25.26 min, extraction temperature of 50 ℃ and microwave power of 600 W. The FRAP, TEAC and TPC values of the extract under the optimal conditions were 381.29 ± 4.42 μM Fe(II)/g dry weight (DW), 613.11 ± 9.32 μM Trolox/g DW and 135.94 ± 0.52 mg gallic acid equivalent (GAE)/g DW, respectively. In addition, the major antioxidant components in the extract were detected by high-performance liquid chromatography with diode array detection (HPLC-DAD), including phlorizin, phloretin and trilobatin. The crude extract could be used as food additives or developed into functional food for the prevention and management of oxidative stress-related diseases.

scholarly journals Optimisation of microwave-assisted extraction of flavonoids and phenolics from celery (Apium graveolens L.) leaves by response surface methodology

2016 ◽  
Vol 34 (No. 4) ◽  
pp. 341-349 ◽  
Author(s):  
Q. He ◽  
Y. Li ◽  
P. Zhang ◽  
A. Zhang ◽  
H. Wu
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Apium Graveolens ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Inhibition Rate ◽  
Microwave Assisted ◽  
Assisted Extraction

The conditions for microwave-assisted extraction (MAE) of total flavonoids (TF) and total phenols (TP), and antioxidant activity from celery (Apium graveolens L.) leaves by response surface methodology (RSM) were optimised. The 3-level, 3-factorial Box-Behnken design (BBD) was employed to study three main extraction conditions: microwave power (300–500 W), solid-solvent ratio (15–30 ml/g), and ethanol concentration (50–80%). It was found that microwave power of 500 W at 30 ml/g solid-solvent ratio with 75.6% (v/v) ethanol concentration was the most optimum conditions for the extraction of TF and TP from celery leaves with the consequent high antioxidant activity measured by the DPPH inhibition rate. Using the optimum extraction conditions, the extraction yields of TF and TP were 0.62 g RUE/100 g DW, 3.01 g GAE/100 g DW, respectively, and the DPPH inhibition rate was 88%. The results indicated that the nutritional quality of celery (Apium graveolens L.) leaves could be improved significantly by optimising the extraction process of MAE using response surface methodology.

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