scholarly journals In vitro evaluation of eight plant essential oils for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papaya

2018 ◽  
Vol 54 (No. 3) ◽  
pp. 153-162 ◽  
Author(s):  
Sarkhosh Ali ◽  
Schaffer Bruce ◽  
Vargas Ana I ◽  
Palmateer Aaron J ◽  
Lopez Patricia ◽  
...  
Keyword(s):  
Essential Oils ◽  
Plant Species ◽  
Major Constituent ◽  
Mycelium Growth ◽  
In Vitro Evaluation ◽  
Tea Tree ◽  
Plant Essential Oils ◽  
Thyme Oil ◽  
Application Rates

In vitro efficacy of the essential oils extracted from eight plant species was tested at application rates of 100, 250, 500, 1000, or 2000 µl/l for controlling fruit rots. Results showed a 100% reduction of mycelium growth of Colletotrichum, Fusarium, Phytophthora, Botryosphaeria after applying thyme or savory oils at all concentrations tested. Mint and cinnamon oils inhibited mycelium growth of the five isolates at application rates of either 1000 or 2000 µl/l. Tea tree, lavender, myrtle, and eucalyptus oils were slightly effective at controlling mycelium growth of each fungus species tested. Savory oil with major constituent of carvacrol 71.2% and thyme oil with major constituent of thymol 73.3% showed the greatest potential of the essential oils tested for use as natural fungicides.

scholarly journals Antimicrobial efficacy of five essential oils against oral pathogens: An in vitro study

2013 ◽  
Vol 07 (S 01) ◽  
pp. S071-S077 ◽  
Author(s):  
Nilima Thosar ◽  
Silpi Basak ◽  
Rakesh N. Bahadure ◽  
Monali Rajurkar
Keyword(s):  
Essential Oils ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Peppermint Oil ◽  
Tea Tree Oil ◽  
Lavender Oil ◽  
Tea Tree ◽  
Thyme Oil ◽  
Oral Pathogens

ABSTRACT Objectives: This study was aimed to find out the minimum inhibitory concentration (MIC) of five essential oils against oral pathogens and to find out the minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) of five essential oils against oral pathogens. Materials and Methods: The antimicrobial activities by detecting MIC and MBC/MFC of five essential oils such as tea tree oil, lavender oil, thyme oil, peppermint oil and eugenol oil were evaluated against four common oral pathogens by broth dilution method. The strains used for the study were Staphylococcus aureus ATCC 25923, Enterococcus fecalis ATCC 29212, Escherichia coli ATCC 25922 and Candida albicans ATCC 90028. Results: Out of five essential oils, eugenol oil, peppermint oil, tea tree oil exhibited significant inhibitory effect with mean MIC of 0.62 ± 0.45, 9.00 ± 15.34, 17.12 ± 31.25 subsequently. Mean MBC/MFC for tea tree oil was 17.12 ± 31.25, for lavender oil 151.00 ± 241.82, for thyme oil 22.00 ± 12.00, for peppermint oil 9.75 ± 14.88 and for eugenol oil 0.62 ± 0.45. E. fecalis exhibited low degree of sensitivity compared with all essential oils. Conclusion: Peppermint, tea tree and thyme oil can act as an effective intracanal antiseptic solution against oral pathogens.

scholarly journals Antifungal Activity of Plant Essential Oils Against Botrytis cinerea, Penicillium italicum and Penicillium digitatum

2013 ◽  
Vol 41 (1) ◽  
pp. 86 ◽  
Author(s):  
Andrew VITORATOS ◽  
Dimitrios BILALIS ◽  
Anestis KARKANIS ◽  
Aspasia EFTHIMIADOU
Keyword(s):  
Essential Oils ◽  
Botrytis Cinerea ◽  
Disease Severity ◽  
Grey Mould ◽  
Penicillium Italicum ◽  
Postharvest Diseases ◽  
Mycelium Growth ◽  
Plant Essential Oils

Plant essential oils have the potential to replace the synthetic fungicides in the management of postharvest diseases of fruit and vegetables.The aim of this study was to access the in vitro and in vivo activity of essential oil obtained from oregano (Origanum vulgare L. ssp. hirtum), thyme (Thymus vulgaris L.) and lemon (Citrus limon L.) plants, against some important postharvest pathogens (Botrytis cinerea, Penicillium italicum and P. digitatum). In vitro experiments indicated that P. italicum did not show any mycelium growth in presence of thyme essential oils at concentration of 0.13 μl/ml. Moreover, B. cinerea did not show any mycelium growth in presence of lemon and oregano essential oils at concentration of 17 μl/ml and 0.02 μl/ml, respectively. Moreover, the essential oils from three species were effective in reducing the spore germination. The in vivo experiments confirmed the strong efficacy shown in vitro by essential oils. These oregano and lemon oils were very effective in controlling disease severity of infected fruit by B. cinera in tomatoes, strawberries and cucumbers. In tomatoes, grey mould due to B. cinerea was completed inhibited by oregano essential oils at 0.30 μl/ml. Moreover, lemon essential oils induced a significant reduction of grey mould disease severity. In strawberries, grey mould was completed inhibited by lemon essential oils at 0.05 μl/ml. In addition, lemon essential oils at 0.05 μl/ml showed 39% reduction of infected cucumber fruits by B. cinerea. These results indicate that essential oils after suitable formulation could be used for the control of postharvest diseases caused by Botrytis and Penicillium pathogens.

scholarly journals Antibacterial activity of essential oils on Xanthomonas vesicatoria and control of bacterial spot in tomato

2012 ◽  
Vol 47 (3) ◽  
pp. 351-359 ◽  
Author(s):  
Gilvaine Ciavareli Lucas ◽  
Eduardo Alves ◽  
Ricardo Borges Pereira ◽  
Fabiano José Perina ◽  
Ricardo Magela de Souza
Keyword(s):  
Essential Oils ◽  
Bacterial Spot ◽  
Powdered Milk ◽  
Tea Tree ◽  
Plant Essential Oils ◽  
Bacterial Morphology ◽  
Severity Of The Disease ◽  
And Control ◽  
Xanthomonas Vesicatoria

The objective of this work was to evaluate the effects of plant essential oils (EOs) on the growth of Xanthomonas vesicatoria, on bacterial morphology and ultrastructure, and on the severity of tomato bacterial spot. EOs from citronella, clove, cinnamon, lemongrass, eucalyptus, thyme, and tea tree were evaluated in vitro at concentrations of 0.1, 1.0, 10, and 100% in 1.0% powdered milk. The effect of EOs, at 0.1%, on the severity of tomato bacterial spot was evaluated in tomato seedlings under greenhouse conditions. The effects of citronella, lemongrass, clove, and tea tree EOs, at 0.1%, on X. vesicatoria cells were evaluated by transmission electron microscopy. All EOs showed direct toxic effect on the bacteria at a 10%-concentration in vitro. Under greenhouse conditions, the EOs of clove, citronella, tea tree, and lemongrass reduced disease severity. EOs of clove and tea tree, and streptomycin sulfate promoted loss of electron-dense material and alterations in the cytoplasm, whereas EO of tea tree promoted cytoplasm vacuolation, and those of citronella, lemongrass, clove, and tea tree caused damage to the bacterial cell wall. The EOs at a concentration of 0.1% reduce the severity of the disease.

scholarly journals In vitro evaluation of the effects of some plant essential oils on Ascosphaera apis , the causative agent of Chalkbrood disease

2017 ◽  
Vol 24 (5) ◽  
pp. 1001-1006 ◽  
Author(s):  
Mohammad Javed Ansari ◽  
Ahmad Al-Ghamdi ◽  
Salma Usmani ◽  
Khalid Ali Khan ◽  
Abdulaziz S. Alqarni ◽  
...  
Keyword(s):  
Essential Oils ◽  
Causative Agent ◽  
Ascosphaera Apis ◽  
In Vitro Evaluation ◽  
Plant Essential Oils

scholarly journals In vitro antimicrobial properties of plant essential oils thymus vulgaris, cymbopogon citratus and laurus nobilis against five important foodborne pathogens

2012 ◽  
Vol 32 (1) ◽  
pp. 167-172 ◽  
Author(s):  
Alessandra Farias Millezi ◽  
Danila Soares Caixeta ◽  
Diogo Francisco Rossoni ◽  
Maria das Graças Cardoso ◽  
Roberta Hilsdorf Piccoli
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Antimicrobial Properties ◽  
Major Constituent ◽  
Thymus Vulgaris ◽  
Cymbopogon Citratus ◽  
Laurus Nobilis ◽  
Plant Essential Oils

Several essential oils of condiment and medicinal plants possess proven antimicrobial activity and are of important interest for the food industry. Therefore, the Minimum Inhibitory Concentrations (MIC) of those oils should be determined for various bacteria. MIC varies according to the oil used, the major compounds, and the physiology of the bacterium under study. In the present study, the essential oils of the plants Thymus vulgaris (time), Cymbopogon citratus (lemongrass) and Laurus nobilis (bay) were chemically quantified, and the MIC was determined on the bacteria Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19117, Salmonella enterica Enteritidis S64, and Pseudomonas aeruginosa ATCC 27853. The essential oil of C. citratus demonstrated bacterial activity at all concentrations tested and against all of the bacteria tested. The majority of essential oil compounds were geranial and neral. The major constituent of T. vulgaris was 1.8-cineol and of L. nobilis was linalool, which presented lower antibacterial activity, followed by 1.8-cineol. The Gram-negative bacteria demonstrated higher resistance to the use of the essential oils tested in this study. E. coli was the least sensitive and was inhibited only by the oils of C. citratus and L. nobilis.

THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

2018 ◽  
Vol 17 (6) ◽  
pp. 167-174 ◽  
Author(s):  
Małgorzata Schollenberger ◽  
Tomasz M. Staniek ◽  
Elżbieta Paduch-Cichal ◽  
Beata Dasiewicz ◽  
Agnieszka Gadomska-Gajadhur ◽  
...  
Keyword(s):  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Mentha Piperita ◽  
Mentha Spicata ◽  
Plant Essential Oils ◽  
Plant Pathogenic Bacteria ◽  
Interspecies Hybrids

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.

scholarly journals Anti-bacterial activity of three essential oils - An in vitro study

2019 ◽  
Vol 10 (2) ◽  
pp. 1049-1053 ◽  
Author(s):  
Geetha RV ◽  
John Rozar Raj B ◽  
Lakshmi Thangavelu
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Streptococcus Mutans ◽  
Bacterial Species ◽  
In Vitro Study ◽  
Oral Bacteria ◽  
Zone Of Inhibition ◽  
Thyme Oil ◽  
Rosemary Oil

To conduct a study regarding the antibacterial activity of essential oils against bacteria causing Caries. Essential oils are distillates of the volatile compounds of a plant’s secondary metabolism and may act as photoprotective agents. Their curative effect has been known since antiquity. It is based on a variety of pharmacological properties which are specific for each plant species. The mouth contains a variety of oral bacteria, but only a few species of bacteria are believed to cause dental caries. Antibacterial activity of the three essential oils, Rosemary oil, Holy basil oil, Thyme oil was screened against Streptococcus mutans, using disc diffusion technique. The rosemary oil was more effective against Streptococcus mutans with a zone of inhibition of 52 mm diameter (at concentration 200 µl), Rosemary oil showed a zone of inhibition of 44 mm diameter and with thyme oil, the zone diameter was 30 mm. The results of this study showed that the essential oils at different concentrations exhibited antibacterial activity against the bacterial species tested.

scholarly journals THE EFFECT OF ESSENTIAL OILS ON MICROORGANISMS CAUSING CARIES AND PERIODONTITIS

2020 ◽  
Vol 20 (2) ◽  
pp. 253-259
Author(s):  
N.M. Devyatkina ◽  
N.O. Bobrova ◽  
E.M. Vazhnichaya
Keyword(s):  
Essential Oils ◽  
Dental Treatment ◽  
Therapeutic Potential ◽  
Oral Care ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Oral Streptococci ◽  
Tea Tree

The oral cavity contains a large number of bacteria, some of which are involved in the development of caries and periodontitis (S. mutans, S. sobrinus, Lactobacilli spp, P. intermedia, P. gingivalis, and T. forythus). The disadvantages of existing antiseptics used in dentistry necessitate the study of antibacterial properties of herbal medicines, and, in particular, of essential oils. The aim of this review is to provide the analysis of literature sources from PubMed and Google Scholar databases related to the effects of essential oils of cloves, mint, thyme, eucalyptus, tea tree and their components on cariogenic and periodontopathic microflora. It was found out that the most in vitro studies evaluated the effects of essential oils or isolated compounds (eugenol, menthol, thymol, carvacrol, eucalyptol, and terpinene-4-ol) on S. mutans, which is considered to be the most cariogenic of oral streptococci, and the researchers limited to defining the susceptibility of the microorganism and effects on biofilm formation. Only in a few studies, the effects of essential oils on the virulence factors of oral pathogens, in particular glycosyl transferase, are represented. Clinical trials of essential oils, their components and combinations confirm the therapeutic potential of these agents in vivo, but raise the question of their effectiveness, taking into account the short-term action, which does not exceed the potency of chlorhexidine. Essential oils of cloves, mint, thyme, eucalyptus, tea tree and their components should be used for treating caries and periodontitis. They are also promising when used as agents of the oral care products, preservatives of the dental medicinal forms, and as remedies for halitosis. With a rational prescription, essential oils can be useful in improving the quality of dental treatment and preventive procedures.

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