In vitro antimicrobial properties of plant essential oils thymus vulgaris, cymbopogon citratus and laurus nobilis against five important foodborne pathogens

Several essential oils of condiment and medicinal plants possess proven antimicrobial activity and are of important interest for the food industry. Therefore, the Minimum Inhibitory Concentrations (MIC) of those oils should be determined for various bacteria. MIC varies according to the oil used, the major compounds, and the physiology of the bacterium under study. In the present study, the essential oils of the plants Thymus vulgaris (time), Cymbopogon citratus (lemongrass) and Laurus nobilis (bay) were chemically quantified, and the MIC was determined on the bacteria Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19117, Salmonella enterica Enteritidis S64, and Pseudomonas aeruginosa ATCC 27853. The essential oil of C. citratus demonstrated bacterial activity at all concentrations tested and against all of the bacteria tested. The majority of essential oil compounds were geranial and neral. The major constituent of T. vulgaris was 1.8-cineol and of L. nobilis was linalool, which presented lower antibacterial activity, followed by 1.8-cineol. The Gram-negative bacteria demonstrated higher resistance to the use of the essential oils tested in this study. E. coli was the least sensitive and was inhibited only by the oils of C. citratus and L. nobilis.

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Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-Resistant Escherichia coli

Microorganisms ◽

10.3390/microorganisms10010109 ◽

2022 ◽

Vol 10 (1) ◽

pp. 109

Author(s):

Mohammadreza Pajohi Alamoti ◽

Behnaz Bazargani-Gilani ◽

Razzagh Mahmoudi ◽

Anna Reale ◽

Babak Pakbin ◽

...

Keyword(s):

Escherichia Coli ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Properties ◽

Multidrug Resistant ◽

Zataria Multiflora ◽

Cuminum Cyminum ◽

Plant Essential Oils ◽

E Coli ◽

Mentha Longifolia

Aim of this study was to investigate the antimicrobial properties of herbal plant essential oils (EOs) from selected Iranian plant species such as Ferulago angulata, Zataria multiflora, Cuminum cyminum, and Mentha longifolia against antibiotic-resistant Escherichia coli (E. coli) strains. For this purpose, the Escherichia coli strains, isolated from raw cow’s milk and local dairy products (yogurt, cream, whey, cheese, and confectionery products) collected from different areas of Hamedan province, Iran, were investigated for their resistance to antibiotics (i.e., streptomycin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin, and cefixime). Thus, the E. coli strains were tested for their susceptibility to the above-mentioned essential oils. Regarding antibiotics, the E. coli strains were highly sensitive to ciprofloxacin. In relation to essential oils, the most effective antibacterial activity was observed with Zataria multiflora; also, the bacteria were semi-sensitive to Cuminum cyminum and Mentha longifolia essential oils. All strains were resistant to Ferulago angulata essential oil. According to the results, the essential oil of Zataria multiflora can be considered as a practical and alternative antibacterial strategy to inhibit the growth of multidrug-resistant E. coli of dairy origin.

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EFFICACY OF FOUR ESSENTIAL OILS ON FUSARIUM HEAD BLIGHT CAUSED BY FUSARIUM OXYSPORUM F. SP. RADICIS LYCOPERSICI (FORL), A FUNGAL TELLURIC PATHOGEN OF TOMATO GROWN UNDER COVER

International Journal of Advanced Research ◽

10.21474/ijar01/13835 ◽

2021 ◽

Vol 9 (11) ◽

pp. 1018-1030

Author(s):

Doumbouya Mohamed ◽

◽

Kouame Koffi Gaston ◽

Brou Kouassi Guy ◽

NGuessan Aya Carine ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Fusarium Oxysporum ◽

Fusarium Head Blight ◽

Growth Parameters ◽

Curative Treatment ◽

Life Stages ◽

Cymbopogon Citratus ◽

Head Blight

Fusarium head blight caused by Fusarium oxysporum f. sp. Radicis-lycopersici (FORL) is a constraint to tomato cultivation in Korhogo, CÃƒÂ´te dIvoire. To control this disease, the antifungal activity of essential oils extracted from certain plants was evaluated in comparison with that of a synthetic fungicide for use. In vitro tests were performed on the different life stages of FORL. The in vivo evaluations consisted in carrying out two modes of treatment (curative and preventive) on plants of a susceptible variety of tomato inoculated with FORL. The results showed that the essential oils significantly reduced the different life stages of F.oxysporum f. sp. Radicis-lycopersici (FORL) as did the synthetic product. Thus, the essential oil Cymbopogon citratus at concentrations of 4000 and 6000 ppm strongly inhibited in vitro the different stages of FORL. In preventive and curative treatment, the essential oils not only improved the growth parameters of tomato plants but also reduced the incidence and severity of diseases. Thus, it was found that Cymbopogon citratus (4000 ppm) in preventive treatment, and Banko plus (250 ppm) in curative treatment reduced the mortality rate of FORL up to 6.66% and improved the growth parameters and reduced the flowering time of the tomato plant.The essential oil of C. citratus could be used for biological control of F. oxysporum f. sp. Radicis lycopersici.

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In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae

International Journal of Molecular Sciences ◽

10.3390/ijms21186736 ◽

2020 ◽

Vol 21 (18) ◽

pp. 6736 ◽

Cited By ~ 1

Author(s):

Miroslava Kačániová ◽

Margarita Terentjeva ◽

Jana Žiarovská ◽

Przemysław Łukasz Kowalczewski

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Digestive Tract ◽

Antagonistic Effect ◽

Paenibacillus Larvae ◽

Thymus Vulgaris ◽

Plant Essential Oils ◽

Microbial Groups ◽

Selection Of

The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae.

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In vitro antibacterial activity of 34 plant essential oils against Alternaria alternata

E3S Web of Conferences ◽

10.1051/e3sconf/201913606006 ◽

2019 ◽

Vol 136 ◽

pp. 06006

Author(s):

Qiyu Lu ◽

Ji Liu ◽

Caihong Tu ◽

Juan Li ◽

Chunlong Lei ◽

...

Keyword(s):

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Essential Oils ◽

Alternaria Alternata ◽

Inhibitory Concentration ◽

Drug Susceptibility ◽

Inhibitory Effect ◽

Plant Essential Oils ◽

Mustard Essential Oil

To determine the antibacterial effect of 34 plant essential oils on Alternaria alternata, 34 plant essential oils such as asarum essential oil, garlic essential oil, and mustard essential oil are used as inhibition agents to isolate A. alternata from citrus as indicator bacteria, through the bacteriostasis test and drug susceptibility test, the types of essential oils with the best inhibitory effect were screened and their concentration was determined. The results showed that the best inhibition effect was mustard essential oil with a minimum inhibitory concentration of 250 μl/L and a minimum bactericidal concentration of 250 μl/L. Followed by the Litsea cubeba essential oil and basil oil, the minimum inhibitory concentration is 500 μl/L.

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In vitro evaluation of eight plant essential oils for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papaya

Plant Protection Science ◽

10.17221/49/2017-pps ◽

2018 ◽

Vol 54 (No. 3) ◽

pp. 153-162 ◽

Cited By ~ 7

Author(s):

Sarkhosh Ali ◽

Schaffer Bruce ◽

Vargas Ana I ◽

Palmateer Aaron J ◽

Lopez Patricia ◽

...

Keyword(s):

Essential Oils ◽

Plant Species ◽

Major Constituent ◽

Mycelium Growth ◽

In Vitro Evaluation ◽

Tea Tree ◽

Plant Essential Oils ◽

Thyme Oil ◽

Application Rates

In vitro efficacy of the essential oils extracted from eight plant species was tested at application rates of 100, 250, 500, 1000, or 2000 µl/l for controlling fruit rots. Results showed a 100% reduction of mycelium growth of Colletotrichum, Fusarium, Phytophthora, Botryosphaeria after applying thyme or savory oils at all concentrations tested. Mint and cinnamon oils inhibited mycelium growth of the five isolates at application rates of either 1000 or 2000 µl/l. Tea tree, lavender, myrtle, and eucalyptus oils were slightly effective at controlling mycelium growth of each fungus species tested. Savory oil with major constituent of carvacrol 71.2% and thyme oil with major constituent of thymol 73.3% showed the greatest potential of the essential oils tested for use as natural fungicides.

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EFFECT OF ESSENTIAL OILS IN THE CONTROL OF THE Clavibacter michiganensis SUBESPECIE michiganensis IN TOMATO (Lycopersicum esculentum L.) PLANTS

Biotecnia ◽

10.18633/biotecnia.v20i3.704 ◽

2018 ◽

Vol 20 (3) ◽

pp. 96-101 ◽

Cited By ~ 2

Author(s):

Jesús Borboa Flores ◽

Jesús Ortega García ◽

Francyelli Regina Costa Becheleni ◽

Arnoldo Villegas Espinoza ◽

Francisco Javier Wong-Corral ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antimicrobial Properties ◽

Thymus Vulgaris ◽

Clavibacter Michiganensis ◽

Origanum Vulgare ◽

Cinnamomum Zeylanicum ◽

Tomato Plants ◽

Clavibacter Michiganensis Subsp ◽

Bactericidal Properties

Plants produce compounds with antimicrobial properties that can be used to control diseases of horticultural products, for this reason, the aim of this study was to enlarge the knowledge on the effect of essential oils as an alternative for control for bacterial canker Clavibacter michiganensis subsp. michiganensis (Smith) (Cmm). Essential oil of Lippia palmeri was obtained by hydridestillation from plants collected in Sonora, Mexico. Essential oils of Origanum vulgare, Thymus vulgaris and Cinnamomum zeylanicum were obtained from commercial stores in Hermosillo, Sonora, Mexico. To evaluate the effect of the essential oils on the bacterium Cmm, direct applications in two concentrations (1:1 and 1:5 (v/v)) on tomato plants foliage were carried out. Then, plants were inoculated with 1x10-7 CFU/mL of the bacterium and observed for 12 days. The evaluated parameters were incidence and severity. The essential oils and chemical compounds of Origanum vulgare and Thymus vulgaris had effect against Clavibacter michiganensis subsp. michiganensis up to 9 days after application in a 1:1 (v/v) concentration and up to 12 days after application with the 1:5 (v/v) concentration. The essential oil of Lippia palmeri only had effect until six days after the application with both concentrations; that is to say, only these three types of essential oils had bactericidal properties, since with the essential oil of Cinnamomum zeylanicum the inhibition of Cmm was very limited, both in incidence as in severity.

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Antifungal Activity and Chemical Composition of Seven Essential Oils to Control the Main Seedborne Fungi of Cucurbits

Antibiotics ◽

10.3390/antibiotics10020104 ◽

2021 ◽

Vol 10 (2) ◽

pp. 104

Author(s):

Marwa Moumni ◽

Gianfranco Romanazzi ◽

Basma Najar ◽

Luisa Pistelli ◽

Hajer Ben Amara ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

In Vivo Studies ◽

Gas Chromatography Mass Spectrometry ◽

Major Constituent ◽

Mycelia Growth ◽

Seedborne Fungi ◽

Seedborne Pathogens

Essential oils represent novel alternatives to application of synthetic fungicides to control against seedborne pathogens. This study investigated seven essential oils for in vitro growth inhibition of the main seedborne pathogens of cucurbits. Cymbopogon citratus essential oil completely inhibited mycelial growth of Stagonosporopsis cucurbitacearum and Alternaria alternata at 0.6 and 0.9 mg/mL, respectively. At 1 mg/mL, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and two Origanum majorana essential oils inhibited mycelia growth of A. alternata by 54%, 71%, 68%, 36%, 90%, and 74%, respectively. S. cucurbitacearum mycelia growth was more sensitive to Lavandula essential oils, with inhibition of ~74% at 1 mg/mL. To determine the main compounds in these essential oils that might be responsible for this antifungal activity, they were analyzed by gas chromatography–mass spectrometry (GC-MS). C. citratus essential oil showed cirtal as its main constituent, while L. dentata and L. nobilis essential oils showed eucalyptol. The M. alternifolia and two O. majorana essential oils had terpinen-4-ol as the major constituent, while for L. hybrida essential oil, this was linalool. Thus, in vitro, these essential oils can inhibit the main seedborne fungi of cucurbits, with future in vivo studies now needed to confirm these activities.

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Balık Hastalıkları ile Mücadelede Bitki Esansiyel Yağlarının Major Komponentlerinin Önemi Üzerine bir Araştırma

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v6i10.1501-1506.2151 ◽

2018 ◽

Vol 6 (10) ◽

pp. 1501

Author(s):

Azime Küçükgül

Keyword(s):

Phenolic Compounds ◽

Essential Oil ◽

Essential Oils ◽

Thymus Vulgaris ◽

Therapeutic Effects ◽

Alpha Pinene ◽

Phytochemical Components ◽

In Vivo Effects

The presence of different phytochemical components of essential oil such as tannins, alkaloids, terpenoids and phenolic compounds has antibacterial, antifungal, and anti-inflammatory effects. The aim of this study is to investigate importance of the major components of three herb essential oils (Thymus vulgaris L., Centauriumerythraea Rafn. And Foeniculumvulgare Mill) on challenging with fish diseases. The components of essential oils provided from a commercial firm were made GC/MS analyzes. The major component of T. vulgarewas carvacrol called as phenol, 2-methyl-5-(1-methylethyl) with 40%.The others were Linalool L (15.11%) and benzene, methyl(1-methylethyl)- (12.12%).The richest oil in C. erythraea was bicyclo[3.1.1]hept-2-ene, 2,6,6-trimethyl- (34.90%) called as alpha-pinene, followed by heptacosane (19.15%) and dotriacontane (17.72%), respectively.The evaluation of the essential oil of F. vulgarispresented benzene, 1-methoxy-4-(1-propenyl)- with 67.99%, followed by dl-Limonene (16.03%) and benzene, 1-methoxy-4-(2-propenyl)- (6.97%). The therapeutic effects of thyme are due to its high content of phenolic compounds, particularly carvacrol. The most important compounds of F. vulgare essential oil is anethole, fenchone, limonene that has antibacterial, antioxidant, antifungal and anticancer effects. The previous studies show that alpha pinene significantly inhibits many pathogenic Gram-negative bacteria. In aquaculture studies, in vitro and in vivo effects of the dominant compounds of essential oils in our study are consistent with the previous findings.

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Spraying of swine buildings with lemon grass (Cymbopogon citratus) essential oil does not produce blood absorption in swine

International Journal of Phytomedicine ◽

10.5138/09750185.1865 ◽

2016 ◽

Vol 8 (3) ◽

pp. 360

Author(s):

Seung-Jin Lee ◽

Ki-Hyun Kim ◽

Abraham Fikru Mechesso ◽

Na-Hye Park ◽

Seung-Chun Park

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Mobile Phase ◽

Hplc Method ◽

Detectable Level ◽

Major Constituent ◽

Cymbopogon Citratus ◽

Blood Samples ◽

Lemon Grass

Cymbopogon citratus (Lemon grass) essential oils have been used in swine buildings to reduce the offensive odor emanating from swine buildings. The present study was designed to investigate plasma residues of citral which is a major constituent of the essential oils of Cymbopogon citratus. An HPLC method was established, validated and used for the determination of citral in swine plasma harvested from blood samples taken at the 14th, 21st and 28th day after spraying swine buildings with the 3% Lemon grass essential oil. Thereafter, analysis of the sample was conducted using HP ODS Hypersil column (200 × 4.6 mm, 5 µm) with a mobile phase consisting of methanol and 0.5% acetic acid; and a flow rate of 1mL/ min. The method was validated for parameters such as accuracy, precision, linearity and detection limits. Plasma spiked with standard citral (95%) revealed two chromatograms with retention times of about 10.7 and 12.2 minutes. The calibration curves for the citral isomers were found to be linear in the tested concentration ranges and mean recoveries wre 101% and 99.17%. This method was used to determine the residues of citral in swine plasma pretreated with methanol. Surprisingly, there was no any detectable level of citral in swine plasma within the 28 days of exposure.spraying of swine buildings with essential oils of lemon grass oil does not cause plasma residues of citral.

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Comparative Study on the Antibacterial Properties of Nanoemulsion of Zataria multiflora Essential Oil Fortified with Cinnamaldehyde Against Common Foodborne Pathogens

The Natural Products Journal ◽

10.2174/2210315509666191111105918 ◽

2020 ◽

Vol 10 (5) ◽

pp. 631-641

Author(s):

Shahnaz Soufi ◽

Majid Aminzare ◽

Hassan Hassanzad Azar ◽

Koorosh Kamali

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Foodborne Pathogens ◽

Salmonella Enteritidis ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Diffusion Method ◽

Suitable Alternative ◽

Zataria Multiflora

Background: Nowadays, the application of several and natural preservatives in small quantity is a more preferred approach. In this regard, one of the effective methods is the formation of nanoemulsion of essential oils. Objective: The objective of this study was to compare the in vitro antibacterial activities of cinnamaldehyde (CIN) and Zataria multiflora essential oil in conventional (ZEO), nanoemulsion (NZEO) and fortified nanoemulsion (NZEOC) forms against common foodborne pathogens. Methods: Firstly, the Zataria multiflora essential oil was analyzed by GC-MS. The nanoemulsion of Z. multiflora essential oil was then prepared alone and fortified with cinnamaldehyde. Finally, their antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Escherichia coli was evaluated. Results: Based on the results, carvacrol (36.62%) was found to be the most important compound of essential oil. In disc diffusion and micro-dilution methods, the addition of CIN to ZEO during nanoemulsion formation (NZEOC) showed more antibacterial activity when compared to the individual addition of NZEO and CIN (NZEO+CIN). However, according to the vapor phase diffusion method, nano-treatments exhibited less inhibitory effects than the other treatments. Conclusion: It can be concluded that the fortification of essential oils with their derived pure compounds during nanoemulsion formation, can be used as a suitable alternative to chemical antibacterial compounds in the food industry.

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