Prevalence and Molecular Characterization of Microbial Contaminants in Raw Cow Milk of Tangail District in Bangladesh

2021 ◽  
Vol 17 ◽  
Author(s):  
Shahin Mahmud ◽  
Md. Firoz Ali ◽  
Md. Omar Faruque ◽  
Muhammad Wasim ◽  
Fatematuz Zuhura Evamoni ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Raw Milk ◽  
Health Impact ◽  
Cow Milk ◽  
Milk Consumption ◽  
Salmonella Spp ◽  
Mean Values ◽  
The Mean ◽  
Quality Of Milk

Objectives: The aim of this study is to evaluate the microbial quality and associated health Methodology: An intensive study was carried out from January¬ to October 2019 at the Tangail district of Bangladesh to assess bacterial contamination of raw milk. A total of 60 samples were examined by following the standard bacteriological methods and the health impact was evaluated with the help of a semi-structured-based questionnaire. Molecular characterization of isolated bacteria was carried out by PCR. Results: Results revealed the mean values of TVC and TCC in raw milk, udder, oil, and utensil samples were 7.6×106, 8.8×106, 4.6×106, and 1.3×106 cfu/ml and 3.7×105, 1.4×106, 4.4×105, and 8.9×104 cfu/ml, respectively. The yeast and mold in raw milk, udder, and oil were 1.5×103, 1.8×103, and 1.3×102cfu/ml, respectively, and the mean values of E. coli in the above-mentioned samples were 1.9×103, 1.8×104, 2.1×103, and 1.6×103cfu/ml, respectively. On the other hand, the mean values of Salmonella spp. in raw milk, udder, oil, and utensil samples were 2.4×102, 7.9×102, 1.5×102, and 1.1×102cfu/ml, respectively. Some selected isolates confirmed by molecular identification were tested for their sensitivity against some common antibiotics used in Bangladesh. Escherichia coli showed 70% resistance to Amoxicillin and 90% sensitivity to Ciprofloxacin whereas Salmonella spp. And Staphylococcus aureus showed 90% resistance to Ampicillin and both were 80% and 90% sensitive to Ciprofloxacin, respectively. Conclusion, Significance, and Impact of Study: Milk producers should be appropriately trained to monitor the overall hygienic conditions surrounding the production and handling of milk until it reaches the consumer. Frequent monitoring of the milk production facility, occasional testing of raw milk, and minimal use of antibiotics will ensure the quality of milk. Consumption of quality milk and avoidance of raw milk consumption will reduce the health risk of the consumers.

scholarly journals Ampelographic and molecular diversity among grapevine (Vitis spp.) cultivars

2009 ◽  
Vol 45 (No. 4) ◽  
pp. 160-168 ◽  
Author(s):  
A. Sabir ◽  
S. Tangolar ◽  
S. Buyukalaca ◽  
S. Kafkas
Keyword(s):  
Molecular Characterization ◽  
Molecular Diversity ◽  
Genetic Distances ◽  
Mean Values ◽  
Issr Analysis ◽  
Geographic Origins ◽  
The Mean ◽  
Vitis Spp

This study presents the ampelographic and molecular characterization of 44 grapevine cultivars. Ampelographic data were obtained during two vegetation periods using the latest version of the descriptors. Based on the mean values transformed by the method indicated in IBPGR publications, a dendrogram was constructed. ISSR analysis was also employed to characterize the genotypes at the DNA level. Twenty primers, selected on the basis of their discriminating potential, generated a total of 157 bands, of which 140 were polymorphic. The dendrograms constructed by the two approaches were largely similar in both the clustering position and divergence of varietal groups. The least distance was observed between Yuvarlak Cekirdeksiz and Superior Seedless. The clustering position of cultivars throughout the dendrograms was basically related to the genetic distances and main uses, as well as to geographic origins.

scholarly journals Aerobic Plate Count of Contaminants and Molecular Characterization of Eschereichia Coli in Raw Chicken Meat in Ismailia, Egypt

2020 ◽  
Vol 2 (2) ◽  
pp. 23-30
Author(s):  
El-Sayed A. Afify ◽  
Fahim A Shaltout ◽  
Zakaria, I. M
Keyword(s):  
Molecular Characterization ◽  
Sensitivity And Specificity ◽  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
Plate Count ◽  
Chicken Breast ◽  
Mean Values ◽  
The Mean ◽  
Aerobic Plate Count

A total number of 100 samples from ten random broiler chicken carcasses (breast and thigh) were collected from an automatic poultry slaughtering plant in Ismailia city, Egypt. The mean values of Enterobacteriacae count were 5.9x104±9.7x103 cfu/g and 7.1x 104 ± 1.1x104 cfu/g for chicken breast and thigh samples respectively. The prevalence of E.coli were 12% and 9% breast and thigh samples examined, respectively. They are serologically identified as 33.35 and 22.2% O157:H7 (EHEC) , 16.6% and 11.1% O114:H21(EPEC), 16.6% and 33.3 %O127:H6 (ETEC) , 0% and 0% O126 (ETEC) and 33.3% and 0% O26 (EHEC) for breast and thigh samples, respectively. The incidence of E.coli O157:H7 was 100% in both serological and PCR methods from biochemical positive E.coli samples. Culture is specific and cheap whereas PCR is sensitive and expensive, hence, we recommend both culture and molecular methods, which improve sensitivity and specificity, to enhance detection of foodborne pathogens including E.coli.

scholarly journals Isolation and Characterization of Bacteriophage ZCSE6 against Salmonella spp.: Phage Application in Milk

Biologics ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 164-176
Author(s):  
Abdallah S. Abdelsattar ◽  
Anan Safwat ◽  
Rana Nofal ◽  
Amera Elsayed ◽  
Salsabil Makky ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Milk ◽  
Salmonella Spp ◽  
Isolation And Characterization ◽  
Food Borne ◽  
Wide Range ◽  
Almost All ◽  
And Control ◽  
Milk And Dairy Products

Food safety is very important in the food industry as most pathogenic bacteria can cause food-borne diseases and negatively affect public health. In the milk industry, contamination with Salmonella has always been a challenge, but the risks have dramatically increased as almost all bacteria now show resistance to a wide range of commercial antibiotics. This study aimed to isolate a bacteriophage to be used as a bactericidal agent against Salmonella in milk and dairy products. Here, phage ZCSE6 has been isolated from raw milk sample sand molecularly and chemically characterized. At different multiplicities of infection (MOIs) of 0.1, 0.01, and 0.001, the phage–Salmonella interaction was studied for 6 h at 37 °C and 24 h at 8 °C. In addition, ZCSE6 was tested against Salmonella contamination in milk to examine its lytic activity for 3 h at 37 °C. The results showed that ZCSE6 has a small genome size (<48.5 kbp) and belongs to the Siphovirus family. Phage ZCSE6 revealed a high thermal and pH stability at various conditions that mimic milk manufacturing and supply chain conditions. It also demonstrated a significant reduction in Salmonella concentration in media at various MOIs, with higher bacterial eradication at higher MOI. Moreover, it significantly reduced Salmonella growth (MOI 1) in milk, manifesting a 1000-fold decrease in bacteria concentration following 3 h incubation at 37 °C. The results highlighted the strong ability of ZCSE6 to kill Salmonella and control its growth in milk. Thus, ZCSE6 is recommended as a biocontrol agent in milk to limit bacterial growth and increase the milk shelf-life.

scholarly journals Isolation, conventional and molecular characterization of Salmonella spp. from newly hatched broiler chicks

AMB Express ◽  
2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mahmoud E. Sedeik ◽  
Nahed A. El-shall ◽  
Ashraf M. Awad ◽  
Sally M. Elfeky ◽  
Mohamed E. Abd El-Hack ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Broiler Chicks ◽  
Salmonella Spp

scholarly journals Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk

2018 ◽  
Vol 38 (8) ◽  
pp. 1577-1583 ◽  
Author(s):  
José C. Ribeiro Júnior ◽  
Pedro I. Teider Junior ◽  
André L.M. Oliveira ◽  
Edson A. Rios ◽  
Ronaldo Tamanini ◽  
...  
Keyword(s):  
Bovine Milk ◽  
Goat Milk ◽  
Raw Milk ◽  
Species Level ◽  
Cow Milk ◽  
Production Potential ◽  
Gram Negative ◽  
Pseudomonas Spp ◽  
Milk Samples ◽  
The Mean

ABSTRACT: Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat’s and cow’s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.

The Service Time Properties of an Unreliable Server Characterize the Exponential Distribution

1994 ◽  
Vol 26 (01) ◽  
pp. 172-182 ◽  
Author(s):  
Z. Khalil ◽  
B. Dimitrov
Keyword(s):  
Exponential Distribution ◽  
Service Time ◽  
Initial Conditions ◽  
Mean Values ◽  
Unreliable Server ◽  
Service Disciplines ◽  
The Mean ◽  
Server Reliability ◽  
Preemptive Repeat Different

Consider the total service time of a job on an unreliable server under preemptive-repeat-different and preemptive-resume service disciplines. With identical initial conditions, for both cases, we notice that the distributions of the total service time under these two disciplines coincide, when the original service time (without interruptions due to server failures) is exponential and independent of the server reliability. We show that this fact under varying server reliability is a characterization of the exponential distribution. Further we show, under the same initial conditions, that the coincidence of the mean values also leads to the same characterization.

Comparison of the Reproducibilities of the Kjeldahl and Dye Binding Methods for Measuring Protein in Milk

1967 ◽  
Vol 50 (3) ◽  
pp. 557-560 ◽  
Author(s):  
J W Sherbon ◽  
Bobbie Hemphill
Keyword(s):  
Raw Milk ◽  
Reaction Flask ◽  
Mean Values ◽  
Milk Samples ◽  
Coefficients Of Variation ◽  
Dye Binding ◽  
Orange G ◽  
Kjeldahl Method ◽  
The Mean

Abstract Protein in milk samples was measured by both the Kjeldahl and Orange G binding methods. Ten samples of pooled raw milk were tested with ten replications of each method. Coefficients of variation were 0.352-3.089% protein for the Kjeldahl method and 0.098-0.661% protein for the dye binding method. The overall means of the two methods were significantly different, but not more than the expected agreement between duplicate Kjeldahl analyses. The position of the reaction flask in the manifold during digestion and distillation affected the Kjeldahl results. One sample of pasteurized-homogenized milk was tested on four successive days. The variation in dye binding results was less than in the Kjeldahl results. The dye binding response decreased each day and the mean values on the first and last days were significantly different

Occurrence of Clostridium perfringens in Milk and Dairy Products

1980 ◽  
Vol 43 (7) ◽  
pp. 536-537 ◽  
Author(s):  
T. A. EL-BASSIONY
Keyword(s):  
Clostridium Perfringens ◽  
Dairy Products ◽  
Ice Cream ◽  
Raw Milk ◽  
Mean Value ◽  
Processed Cheese ◽  
Mean Values ◽  
Milk Products ◽  
The Mean ◽  
Milk And Dairy Products

A total of 163 samples of milk and milk products (including raw milk, Damietta and Kareish cheese, hard and processed cheese, yogurt, butter and ice cream) were collected from Assiut City markets. Clostridium perfringens was detected in 45.5, 20, 30, 14.3, 20, 10, 50 and 20% of the examined samples, respectively, using SPS agar. With TSN agar, the organism was isolated from 72.7, 20, 30, 28.6, 50, 10, 50, and 30% of the samples, respectively. The mean value of the C. perfringens count using SPS agar was 53.6/ml, 40/g, 60/g, 14.3/g, 30/g, 1.4/g, 25/g and 8/ml of raw milk, Damietta and Kareish cheese, hard and processed cheese, yogurt, butter and ice cream samples, respectively, while the mean values of C. perfringens counts obtained with TSN agar were 76.4/ml, 170/g, 140/g, 42.9/g, 590/g, 2.9/g, 172.9/g, and 109/ml of examined samples, respectively. Milk and dairy products, if improperly handled, provide a means for transmission of C. perfringens to the consumer.

Molecular characterization of Aspergillus fumigatus isolated from raw cow milk in Argentina

2018 ◽  
Vol 275 ◽  
pp. 1-7 ◽  
Author(s):  
Gabriela A. Pena ◽  
Verónica Alonso ◽  
Marina Velasco Manini ◽  
M. Pellegrino ◽  
Lilia R. Cavaglieri
Keyword(s):  
Aspergillus Fumigatus ◽  
Molecular Characterization ◽  
Cow Milk

scholarly journals A METHODOLOGICAL APPROACH TO THE CHARACTERIZATION OF BRAIN GLIOMAS, BY MEANS OF SEMI-AUTOMATIC MORPHOMETRIC ANALYSIS

2014 ◽  
Vol 33 (3) ◽  
pp. 201 ◽  
Author(s):  
Artur Dawid Surowka ◽  
Dariusz Adamek ◽  
Edyta Radwanska ◽  
Marek Lankosz ◽  
Magdalena Szczerbowska-Boruchowska
Keyword(s):  
Aspect Ratio ◽  
Digital Image Analysis ◽  
Methodological Approach ◽  
Morphometric Parameters ◽  
Mean Values ◽  
The Mean ◽  
Analytical Tools ◽  
Brain Gliomas

The aims of this paper were to present a reliable morphometric procedure for glioma analysis for preliminary prognosis and to develop a semi-automatic procedure that is easy to use. The data presented are important to the extent that they verify the reliability of the results by showing that they are consistent with the findings from more complicated automatic analytical tools. The objects for analysis were digital images of haematoxylin-eosin stained glioma samples. The overall analysis consisted of digital image analysis and the determination of morphometric parameters. Interestingly, an increase in the mean values of aspect ratio with increasing malignancy grade was found. Moreover, the morphometric parameters in relation to the histological origin of gliomas were examined and it was found that, the cellular nuclei of glioblastoma multiforme reveal the biggest mean values of aspect ratio compared with other gliomas.

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