scholarly journals Determination of haematological and biochemical parameters of Przewalski horses (Equus przewalski) kept by the Prague Zoo

2014 ◽  
Vol 59 (No. 1) ◽  
pp. 11-21 ◽  
Author(s):  
J. Tomenendalova ◽  
R. Vodicka ◽  
I. Uhrikova ◽  
J. Doubek
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Total Bilirubin ◽  
Age Groups ◽  
Inorganic Phosphorus ◽  
The Czech Republic ◽  
Eosinophilic Granulocytes ◽  
Domestic Horses ◽  
Lower Count

The objective of this study was to determine ranges of haematological and biochemical parameters of Przewalski horses (Equus przewalski) kept in the Czech Republic, to compare the results with data reported for the same species as well as for domestic horses (Equus caballus), and to evaluate differences between both genders and age groups. Stallions showed significantly higher counts of red blood cells and bands, higher levels of total bilirubin and magnesium, and a higher activity of GGT. Higher concentrations of cholesterol, vitamin A and sodium were found in mares. Young animals were characterised by a lower count of eosinophilic granulocytes, a higher activity of ALP and higher concentrations of calcium, inorganic phosphorus, sodium and creatinine. Other differences were not significant.  

scholarly journals Haematological and biochemical parameters in the blood of an indigenous Croatian breed – Istrian cattle

2014 ◽  
Vol 57 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Martina Bedenicki ◽  
Dalibor Potocnjak ◽  
Ivica Harapin ◽  
Berislav Radisic ◽  
Marko Samardzija ◽  
...  
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Metabolic Profile ◽  
Total Bilirubin ◽  
Adult Population ◽  
Inorganic Phosphorus ◽  
Physiological Status ◽  
Differential Analysis ◽  
Physiological Range

Abstract. Haematological and biochemical parameters were examined in the blood of 87 cows, bulls and oxen of the indigenous Croatian breed, Istrian cattle. The sample represents 11.93 % of the total adult population. This breed is classified as highly endangered. The age of tested animals was between 2 and 17 years. Cattle were divided into three groups, animals aged 2 to 6 years (n=19), 7 to 10 years (n=37), and older than 10 years (n=31). Animals were kept in the area of Istria County, mostly in stables though occasionally at pasture. The cows were milked twice daily and gave an average of 1 000 litres of milk per year. Erythrocytes, leukocytes, haemoglobin, haematocrit and mean corpuscular volume were determined. Differential analysis was performed by counting blood cells in the blood smear after Pappenheim staining. Biochemical parameters such as total cholesterol, triglycerides, urea, creatinine, total bilirubin, calcium and inorganic phosphorus were determined. The activity of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase was determined by spectrophotometry. These biochemical and haematological parameters of this Istrian breed were within the physiological range, while slight deviations were observed for certain parameters. The greatest deviation from the physiological range was shown in the concentrations of urea and creatinine. These results may contribute to a better understanding of the metabolic profile and haematological indicators for estimating the physiological status of these endangered cattle in Istria, for further investigation and for diagnostic purposes.

scholarly journals Liver damage with measles

2020 ◽  
Vol 12 (4) ◽  
pp. 78-86
Author(s):  
M. A. Sayfullin ◽  
N. N. Zvereva ◽  
E. A. Nurmuhametova ◽  
N. P. Blohina ◽  
E. Yu. Pylaeva ◽  
...  
Keyword(s):  
Liver Damage ◽  
Medical Records ◽  
Biochemical Parameters ◽  
Blood Test ◽  
Retrospective Cohort ◽  
Total Bilirubin ◽  
Comparison Group ◽  
Risk Groups ◽  
Bilirubin Concentration

Objective: to determine the incidence of liver damage with measles, the specificity of these changes, to identify risk groups, to assess the dynamics of the development of hyperfermentemia in various stages of the disease.Materials and methods: a retrospective cohort study of medical records of patients hospitalized with measles (n = 101), divided into 4 groups: children (n = 23), men (n = 32), women (n = 24) and pregnant women (n = 22). Patients with laboratory-confirmed influenza (n = 61) were taken as a comparison group. The analysis of the frequency of detecting changes in biochemical parameters, average values, standard error and confidence intervals.Results: An increase in ALT in measles patients was observed in 56 (55.4%), above 5 norms – in 15 patients (14.8%), an increase in AST was detected in 80 (79.2%) patients, above 5 norms – in 10 (9.9%). These changes are specific for measles, which was established by comparing the indicators of patients with measles and influenza. The greatest frequency and severity of the observed changes was observed in men. Determination of bilirubin concentration was carried out in 95 patients. An increase in total bilirubin above 20 μmol / L was observed in 10 (10.5%), direct above 5 μmol / L – in 13 (13.6%) patients.Conclusion: given the frequency of occurrence of the identified changes, it is advisable to conduct a biochemical blood test for measles patients. Therapeutic tactics should be determined taking into account the possible development of hepatitis with measles. 

Microchemical analysis for 13 constituents of plasma from healthy children.

1979 ◽  
Vol 25 (5) ◽  
pp. 692-698 ◽  
Author(s):  
M H Cheng ◽  
A I Lipsey ◽  
V Blanco ◽  
H T Wong ◽  
S H Spiro
Keyword(s):  
Total Bilirubin ◽  
Normal Values ◽  
Chemical Constituents ◽  
Age Groups ◽  
Inorganic Phosphorus ◽  
Data Management System ◽  
Healthy Children ◽  
Total Calcium ◽  
Total Proteins ◽  
Microchemical Analysis

Abstract Normal values for 13 chemical constituents of plasma were estimated from results for 837 presumably healthy children. Ninety microliters of specimen was analyzed for lactate dehydrogenase, aspartate aminotransferase, alkaline phosphatase, inorganic phosphorus, total calcium, total cholesterol, total proteins, albumin, uric acid, urea nitrogen, alanine aminotransferase, total bilirubin, and glucose. We used two Abbott ABA-100 Bichromatic Analyzers interfaced directly to the ABA Data Management System. For each test age- and sex-related variations were assessed and normal values were estimated for six different age groups.

scholarly journals HEMATOLOGICAL AND SERUM BIOCHEMICAL PROFILE IN EAST BALKAN PIGS AT DIFFERENT AGE AND SEASONS

AGROFOR ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Nadezhda PALOVA ◽  
Yordan MARCHEV ◽  
Radka NEDEVA ◽  
Jivko NAKEV ◽  
Dimitrinka KRUSHEVA ◽  
...  
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Age Groups ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Biochemical Profile ◽  
Corpuscular Hemoglobin ◽  
Serum Biochemical ◽  
Serum Biochemical Profile

Twelvehematological and fifteen serum biochemical parameters were determined in indigenous East Balkan pigs at different ages and two seasons. The experiment was carried out in the Scientific Centre of Agriculture- Sredets aiming to characterize the health status of the animals when reared organically. Blood samples were taken from pre-weaned piglets (n=10), growers (n=10) and sows (n=10) in spring and summer. The results were analysed through two way ANOVA to assess the influence of the age, season and their interaction on the hematological and serum biochemical profile of the animals. Both age and season interacted significantly in regard to the red blood cells count (RBC) (P0.001), hemoglobin (HGB) (P0.001) and hematocrit (HCT) (P0.01), as well as in the most biochemical parameters including creatinine(P0.001), total protein (TP) (P0.001), albumin (ALB) (P0.001), alanine aminotransferase (ALT) (P0.001), aspartate aminotransferase (AST) (P0.01), uric acid (UA) (P0.01), Mg (P0.001), triglycerides (TG) (P0.05) and cholesterol (P0.01). Regardless of the season, the white blood cells (WBC) including lymphocytes and granulocytes, as well as platelets (PLT)decreased with age, while mean corpuscular volume (MCV) increased.The content of glucose, urea, gamma-glutamil transferase (GGT), alkaline phosphatase (ALP), Ca and P which displayed maximal values in preweaned pigs (P0.05) compared to the other age groups (P0.05). The season affected the number of lymphocytes (P0.01), their percent (P0.001), mean corpuscular hemoglobin (MCH) andmean corpuscular hemoglobin concentration (MCHC) (P0.001), as well as the concentration of GGT (P0.001) which increased during summer,and also granulocytes (P0.01), their percentage (P0.001), PLT (P0.05), glucose, urea, ALP, Ca (P0.001) and P (P0.01) whichwere higher in spring.

scholarly journals Influence of age and sex on certain serum biochemical parameters of dromedary camels

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Nawal Mohamed Elkhair
Keyword(s):  
Reference Values ◽  
Adult Male ◽  
Biochemical Parameters ◽  
Reference Range ◽  
Venous Blood ◽  
Age Groups ◽  
Serum Biochemical Parameters ◽  
Serum Biochemical ◽  
Strong Electrolytes

Background: The identification of the reference values of certain serum biochemical parameters in relation to the age was studied in dromedary camels.Methods: Fourteen healthy young camels (age: 3-5 months), 12 adult male and 10 female camels (age: 5-8 years) were used. Venous blood samples were collected for the determination of certain serum parameters.Results: The reference range ( ± s×1.96) of serum- [Na+] was 142-160 mmol/l and the percentile range (median (1. - 3.Quartile)) was 143-156 mmol/l for all age groups. The reference values of serum- [K+] showed a significant (P<0.05) variation between the age groups being higher the young camels (4.2-7.4 mmol/l) compared to the adult groups (3.7-5.5 mmol/l). Serum- [Protein] and serum- [Albumin] showed significant (P<0.05) differences between the age groups. Significantly higher (P<0.05) reference range of 51-74 g/l and a percentile range of 59-67.7 g/l was observed in the adult female camels compared to young and adult male groups.Conclusion: These reference values are useful in detecting the influence of strong electrolytes such as Na+, K+ and Cl- and weak acids (proteins) on the physiological and pathological status of the camels in relation to their age.Keywords: Camels, age, electrolyte concentrations, total protein, albumin, reference values.

DETERMINATION OF WHITE BLOOD CELLS USING FOLDSCOPE WITH SMARTPHONE

2019 ◽  
pp. 10-13
Author(s):  
Ranu Kumar ◽  
Prasad Kapildeo
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Healthcare Services ◽  
Field Level ◽  
Morphology Analysis

We are traditionally used Microscope in clinical laboratory for determination of white blood cells of human blood smear. Now, in this study we were used Foldscope with Smartphone in the place of Microscope and examine many samples of human blood smear which was collected from local diagnostic centers. We were very easily quantity & morphology analysis of all types of WBC cells such as Neutrophils, Lymphocytes, Monocytes, Eosionophils, Basophils in blood smear with the help of Foldscope & image taken by Smartphone. The main objective of this study is to use Foldscope for quantity & morphology analysis of human WBCs at field level especially poor resource area where healthcare services or centers is not available & where carry of microscope is not possible.

PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI

2018 ◽  
Vol 3 (2) ◽  
pp. 52-61
Author(s):  
Dzikra Arwie ◽  
Islawati
Keyword(s):  
Data Analysis ◽  
Statistical Analysis ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Field Of View ◽  
Laboratory Observation ◽  
Number Of Cells

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.  

Determination of Inorganic Phosphorus in Oxalate Extracts of Soils

1977 ◽  
Vol 41 (1) ◽  
pp. 148-149 ◽  
Author(s):  
L. B. Owens ◽  
D. W. Nelson ◽  
L. E. Sommers
Keyword(s):  
Inorganic Phosphorus
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