scholarly journals Potential antimicrobial effects of pharmacognostic drugs

2003 ◽  
Vol 3 (4) ◽  
pp. 16-22 ◽  
Author(s):  
Elvira E. Kovač-Bešović ◽  
Kemal Durić ◽  
Fahra Bašić ◽  
Džemo Subašić ◽  
Jasminka Imamović
Keyword(s):  
Antimicrobial Activities ◽  
Scientific Discipline ◽  
Point Of View ◽  
Dilution Method ◽  
Plant Materials ◽  
Antimicrobial Effects ◽  
Layer Chromatography ◽  
Microbiological Analyses ◽  
Number Of Bacteria

Pharmacognostic aspect, in treating of natural medicinal materials and medicinal of natural origin, is presented by very complex group of analytical and applicative methods. They are applied by means of identification, purities and quality of natural medicinal materials of vegetal, animal or mineral origin, more precisely of pharmacognostic drugs. A great number of drugs and their isolated products show the antimicrobial activities. The chemical substances, which are present in drugs, are currier of antimicrobial activities manifested by action on different bacteria, fungus, parasites and even viruses. The whole series of methods for analyzing of antimicrobial effects of drugs and the medicines in general are treated by scientific discipline microbiology. In order to complete the pharmacognostic analyses of drugs and there medicinal products, the microbiological analyses take a very important role. The antimicrobial and antifungal effects of series of plant materials, of pharmacognosticdrugs against the large number of bacteria and fungus Candida have been carried out. The drugs we analyzed were treated from their chemical point of view and that is the presence of: coumarins, mucus and anthraquinones. The contents of these substances in plant material were analyzed by method of thin layer chromatography that was published preliminary. The antimicrobial effects of analyzed drugs were determinate by using dilution method in antimicrobial analyses which have been carried out with decocts of drugs.

Inhibitory Effect of Commercial Green Tea and Rosemary Leaf Powders on the Growth of Foodborne Pathogens in Laboratory Media and Oriental-Style Rice Cakes

2009 ◽  
Vol 72 (5) ◽  
pp. 1107-1111 ◽  
Author(s):  
SUN-YOUNG LEE ◽  
SO-YOUNG GWON ◽  
SEUNG-JU KIM ◽  
BO KYUNG MOON
Keyword(s):  
Green Tea ◽  
Foodborne Pathogens ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Enterobacter Sakazakii ◽  
Inhibitory Effects ◽  
Plant Materials ◽  
Rice Cake ◽  
Rice Cakes

The antimicrobial effects of green tea and rosemary added to foods as antagonists to foodborne pathogens were determined in laboratory media and oriental-style rice cakes. The growth of each pathogen (Bacillus cereus, Salmonella, Typhimurium, Enterobacter sakazakii, Escherichia coli O157:H7, Staphylococcus aureus, and Listeria monocytogenes) in tryptic soy broth or rice cake with or without addition of green tea or rosemary leaf powders before autoclaving or cooking, respectively, was investigated after inoculation. The addition of 1% green tea or rosemary produced similar results for inhibiting the growth of pathogens in tryptic soy broth. However, green tea was more effective than rosemary for inhibiting the growth of L. monocytogenes. Both botanicals had inhibitory effects against all pathogens tested in this study. Green tea was particularly effective against B. cereus, S. aureus, and L. monocytogenes, and rosemary was strongly inhibitory against B. cereus and S. aureus. The addition of 1 or 3% green tea or rosemary to rice cakes did not significantly reduce total aerobic counts; however, levels of B. cereus and S. aureus were significantly reduced in rice cakes stored for 3 days at room temperature (22°C). The order of antimicrobial activities against B. cereus in rice cake was 1% rosemary < 1% green tea < 3% rosemary = 3% green tea. These results indicate that the use of natural plant materials such as green tea and rosemary could improve the microbial quality of foods in addition to their functional properties.

scholarly journals Hygienic Conditions and Quality of Dishes Cooked and Sold by Women Traders in the Town of Korhogo

2020 ◽  
pp. 1-10
Author(s):  
Karamoko Detto ◽  
Yoro Dezay Thierry ◽  
Moroh Jean-Luc Aboya ◽  
Koffi Alex Joel ◽  
Djè Koffi Marcellin
Keyword(s):  
Point Of View ◽  
Ishikawa Diagram ◽  
Hygiene Practices ◽  
The One ◽  
Cleaning And Disinfection ◽  
The Town ◽  
Microbiological Analyses ◽  
Good Hygiene ◽  
Hygienic Conditions

The aim of this study is to evaluate the hygienic quality of the dishes cooked and sold by the women traders in the town of Korhogo. The study was carried out with 45 restaurants in the town of Korhogo. It consisted on the one hand, a survey based on the Ishikawa diagram in order to assess the hygienic conditions, and on the other hand of microbiological analyses of the ready-to-serve meals. A total of 90 samples were taken, including 2 dishes per restaurant, respectively consisting of one hot and one cold dish. Generally, results showed that hygienic conditions were unsatisfactory based on the utilized guidelines. From a microbiological point of view, the meal samples were 75.2% satisfactory, 7.6% acceptable and 17.2% unsatisfactory. In view of these results, it appears essential to improve hygiene conditions by greater involvement of the official services involved in training these traders and monitoring compliance with good hygiene practices and the establishment of a program of cleaning and disinfection of premises and equipment.

scholarly journals Assessment of the quality of peppermint leaves by thin-layer chromatography in some objects of plant origin

2020 ◽  
Vol 19 (5-6) ◽  
pp. 148-154
Author(s):  
Vali A. Sahratov ◽  
Tamara L. Malkova ◽  
Ludmila N. Karpova ◽  
Anna A. Pospelova
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Biologically Active ◽  
Mentha Piperita ◽  
Plant Origin ◽  
Plant Materials ◽  
Layer Chromatography ◽  
Active Substances

The State Pharmacopoeia of the XIV edition defines the approach for the assessment of the quality of medicinal plant materials, it deals with the identification of the main groups of biologically active substances by thin layer chromatography. According to this approach, the analysis of some types of medicinal plant materials as a part of some objects of plant origin was carried out. The article presents the quality assessment algorithm as examplified by peppermint leaves (Mentha piperita L.), which are part of a variety of plant object.

scholarly journals High Performance Thin Layer Chromatography: A Qualitative Biochemical Analysis of Sammoha Loha Vati Formulations

2021 ◽  
Vol 9 (02) ◽  
Author(s):  
AJAY PAGHDAL
Keyword(s):  
High Performance ◽  
Biochemical Analysis ◽  
Antimicrobial Activities ◽  
Phase 3 ◽  
Plant Materials ◽  
Simple Tool ◽  
Regulatory Frameworks ◽  
Raw Plant Materials ◽  
Layer Chromatography ◽  
Hptlc Method

Introduction- HPTLC uses include phytochemical and biochemical analyzes, ayurvedic medicine quantification and quantification of active ingredients, formational fingerprinting and adulterant testing of formulations. HPTLC can be used as a simple tool for tracking the consistency of plant-based raw plant materials and formulations. The source of many biomarkers is Sammoha Loha Vati. The HPTLC method has been used to classify and measure the bark of leaves and branches. Aim & Objective- Identification and authentication of raw drugs used for Sammoha Loha Vati through HPTLC. Observations- 1. Stationary phase, 2. Mobile phase, 3. Optimization, 4. Preparation of Sample and its use, 5. Separation, 6. Detection. Conclusion and Finding- The use of HPTLC for the screening of pharmaceutical compounds for antimicrobial activities is emerging. Requirements for the testing of new incoming products and their integration into regulatory frameworks are of great significance for the future of HPTLC. Rf Value having at 254 nm Chromatogram, Spot No. [1] Track T1 [0.22], Track T2 [0.22], Rf Value having at 366 nm Chromatogram, Spot No. [1] Track T1 [0.10], Track T2 [0.10], Rf Value having at 540 nm Chromatogram, Spot No.[1] Track T1 [0.10], Track T2 [0.10].

scholarly journals Monitoring of microscopic fungi community in selected bee products

10.5219/1405 ◽  
2020 ◽  
Vol 14 ◽  
pp. 1105-1114
Author(s):  
Zuzana Mašková ◽  
Vladimíra Kňazovická ◽  
Veronika Mančíková ◽  
Dana Tančinová ◽  
Zuzana Barboráková
Keyword(s):  
Water Activity ◽  
Point Of View ◽  
Dilution Method ◽  
Honey Sample ◽  
Microscopic Fungi ◽  
Human Diet ◽  
Important Indicator ◽  
Isolation Frequency ◽  
Penicillium Spp

Honey is a remarkably complex food with a valued place in the human diet. An important indicator of its quality is the presence of microorganisms. This study aimed to monitor the mycological quality of 27 samples of Slovak kinds of honey and honey products with the addition of differently processed blueberries, cranberries, and red currants. Yeast and filamentous microscopic fungi were monitored using the plate dilution method. A total of 21 samples (78%) were positive for the presence of yeasts and 14 samples (52%) were positive for the filamentous microscopic fungi occurrence. In 6 samples (22%) no presence of microscopic fungi was found at all. The highest number of yeasts (3.07 log CFU.g-1) was recorded in one flower honey sample and in other samples, yeast counts did not exceed 3 log CFU.g-1. The highest numbers of filamentous micromycetes (2.39 and 2.44 log CFU.g-1) were recorded in 2 honeydew honey samples. Overall, the following genera have been identified: Alternaria, Arthrinium, Aspergillus (including previously named as Eurotium), Aureobasidium, Cladosporium, Mucor, Penicillium, and Stemphilium. Penicillium spp. were recorded with the highest isolation frequency (41%). Aspergillus species were isolated from 19% of honey samples. In the honey with fruit addition, the yeasts in a range of 1.00 – 3.09 log CFU.g-1 and the filamentous microscopic fungi in a range of 1.00 – 1.39 log CFU.g-1 were found. The study showed that cranberries were the most appropriate addition from a mycological point of view. Dried and lyophilized forms of tested fruits were the most suitable. Except for honey with frozen currants and honey with fresh cranberries, all final products had a water activity below 0.610 and appeared to be stable.

The microbiological quality of minced pork treated with garlic in combination with vacuum packaging

10.5219/1585 ◽  
2021 ◽  
Vol 15 ◽  
pp. 453-459
Author(s):  
Simona Kunová ◽  
Peter Haščík ◽  
Ľubomír Lopašovský ◽  
Miroslava Kačániová
Keyword(s):  
Microbiological Quality ◽  
Vacuum Packaging ◽  
Coliform Bacteria ◽  
Dilution Method ◽  
Microbial Quality ◽  
Pseudomonas Spp ◽  
Average Value ◽  
Garlic Oil ◽  
Microbiological Analyses

The present study aimed to evaluate the microbial quality of minced pork treated with fresh garlic, dried garlic, and garlic oil in combination with vacuum packing. The growth of Total Viable Counts (TVC), Coliform Bacteria (CB), and Pseudomonas spp. were evaluated. The microbiological analyses were performed by the plate dilution method. The average value of TVC was 8.45 log CFU.g-1 in aerobically packed samples, 5.59 log CFU.g-1 in samples treated with garlic oil, 5.36 log CFU.g-1 in vacuum packed samples, and samples treated with dried garlic, and 4.98 log CFU.g-1 in samples treated with fresh garlic on 8th day of storage. The number of TVC was significantly lower in samples treated with fresh garlic compared to samples treated with dried garlic and garlic oil on the 8th day of storage (p <0.05). The average value of CB was 4.13 log CFU.g-1 in aerobically packed samples, 1.82 log CFU.g-1 in samples treated with garlic oil, the value of CB in vacuum packed samples, in samples treated with fresh garlic and also with dried garlic was lower than 1.00 log CFU.g-1 on 8th day of storage. The number of CB was significantly higher in aerobically packed samples and samples treated with garlic oil compared to vacuum-packed samples, samples treated with fresh garlic and dried garlic on the 8th day of storage (p <0.05). The average number of Pseudomonas spp. was 2.45 in aerobically packed samples, count of Pseudomonas spp. was lower than 1.00 log CFU.g-1 in vacuum packed samples and in the sample with garlic on the 8th day of storage. The number of Pseudomonas spp. was significantly higher in aerobically packed samples in comparison with vacuum-packed samples, samples with fresh garlic, dried garlic, and garlic oil (p <0.05).

scholarly journals Influence of environmental conditions on plants in different climatic zones

2021 ◽  
Vol 845 (1) ◽  
pp. 012148
Author(s):  
G E Larina ◽  
L G Seraya ◽  
S A Golimbovskaya ◽  
I N Kalembet ◽  
T A Petrovnina
Keyword(s):  
Streptomyces Griseus ◽  
Phytopathogenic Fungi ◽  
Point Of View ◽  
Fusarium Avenaceum ◽  
Frequency Of Occurrence ◽  
Fusarium Spp ◽  
Plant Materials ◽  
Climatic Zones ◽  
Climate Studies

Abstract Due to global shifts in climate, studies in contrasting conditions of two natural and climatic zones are of particular interest: (1) subzone of mixed and broad-leaved forests, (2) south-steppe subzone. In the period 2019-2020, 308 plant species from 56 families were surveyed. Phytopathogens damage plants, and therefore increase the loss of quality of plant materials. The frequency of occurrence of mycoses in autumn is 2-14 times higher than in spring-summer observations. The activity of phytopathogenic fungi of the genera of Fusarium (the causative agent of fusarium), Puccinia (rust), Phoma (phomosis), Rhizoctonia (scab), Verticillium (tracheomycotic or vascular wilting) increases. Differences in dominant micromycetes from the point of view of geography were determined: zone 1 (forest) - Alternaria spp., Chaetomium spp., Fusarium spp., F. avenaceum (Fr.) Sacc., (Mart.) Sacc., Heterosporium iridis (Fautrey & Roum.) JE Jacques, Phoma spp., Stemphylium spp., Verticillium spp.; zone 2 (steppe) – Alternaria spp., Botrytis spp., Cladosporium spp., Fusarium avenaceum, Fusarium spp., Peronospora spp., Phoma spp., Pullularia spp., Septoria spp. Screening of pesticides (biological fungicides) showed the effectiveness of preparations based on Pseudomonas fluorescens, Bacillus subtilis or Streptomyces griseus in limiting the growth of the mycelium of phytopathogenic fungi.

scholarly journals Synergistic Potential of the Leaves of Aspillia africana (Compositae) and Psidium guajava (Myrtaceae) Against Some Selected Bacterial and Fungal Isolates

2020 ◽  
Vol 6 (2) ◽  
pp. 34-40
Author(s):  
Foluso Ologundudu ◽  
OLADELE IDRIS
Keyword(s):  
Plant Extracts ◽  
Psidium Guajava ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Significant Activity ◽  
Plant Materials ◽  
Fungal Isolates ◽  
Staphyloccocus Aureus ◽  
Bacteria And Fungi

Background: The plethora of secondary metabolites inherent in plants that could be synthesized and formulated into useful drugs for the treatment of diseases is not fully understood. This study was carried out to evaluate the antimicrobial potential of the leaves of Aspillia africana and Psidium  guajava plants on some selected bacterial and fungal isolates.Methodology: Fresh and matured leaves of Aspillia africana and Psidium guajava were collected at the Federal College of Agriculture, Akure and Obafemi Awolowo University (OAU), Ile-Ife. The plants were identified and voucher deposited at the Herbarium unit of the Department of Botany, Obafemi Awolowo University, Ile-ife. The pure isolates of the bacteria and fungi were obtained from Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria. The bacteria isolates were maintained on nutrient agar slant and the fungal isolates on PDA. Extraction of the plant materials using methanol, ethanol and aqueous fractions were carried out using standard protocols. The isolates were inoculated on a nutrient broth. Minimum Inhibitory Concentration (MIC) of the extracts was determined by broth dilution method while minimal bactericidal and fungicidal concentrations respectively were determined following established protocols.Results: The results showed that Bacillus cereus, Cornybacterium pyogenes, Klebsiella pneumonia and Escherichia coli were all resistant to the plant extracts. The fungal isolates; Candida albicans, Tryptophyton rubrum, Penicillium expansium, and Aspergillus flavus were resistant to Psidium guajava extract as no significant activity was observed. All the plant extracts produced considerable antimicrobial activities with Streptococcus faecalis and Staphyloccocus. aureus. whereas, these organisms were resistant to ofloxacin, sparfloxacin, chloramphenicol, augmentin, ciprofloxacin and septrin.Conclusion: This study revealed that the therapeutic properties of Aspillia africana and Psidium guajava are better enhanced with their synergistic potential to the tested microorganisms.Keywords: Aspillia africana; Bactericidal; Psidium guajava; Synergistic;therapeutic.Background: The plethora of secondary metabolites inherent in plants that could be synthesized and formulated into useful drugs for the treatment of diseases is not fully understood. This study was carried out to evaluate the antimicrobial potential of the leaves of Aspillia africana and Psidium  guajava plants on some selected bacterial and fungal isolates.Methodology: Fresh and matured leaves of Aspillia africana and Psidium guajava were collected at the Federal College of Agriculture, Akure and Obafemi Awolowo University (OAU), Ile-Ife. The plants were identified and voucher deposited at the Herbarium unit of the Department of Botany, Obafemi Awolowo University, Ile-ife. The pure isolates of the bacteria and fungi were obtained from Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Nigeria. The bacteria isolates were maintained on nutrient agar slant and the fungal isolates on PDA. Extraction of the plant materials using methanol, ethanol and aqueous fractions were carried out using standard protocols. The isolates were inoculated on a nutrient broth. Minimum Inhibitory Concentration (MIC) of the extracts was determined by broth dilution method while minimal bactericidal and fungicidal concentrations respectively were determined following established protocols.Results: The results showed that Bacillus cereus, Cornybacterium pyogenes, Klebsiella pneumonia and Escherichia coli were all resistant to the plant extracts. The fungal isolates; Candida albicans, Tryptophyton rubrum, Penicillium expansium, and Aspergillus flavus were resistant to Psidium guajava extract as no significant activity was observed. All the plant extracts produced considerable antimicrobial activities with Streptococcus faecalis and Staphyloccocus. aureus. whereas, these organisms were resistant to ofloxacin, sparfloxacin, chloramphenicol, augmentin, ciprofloxacin and septrin.Conclusion: This study revealed that the therapeutic properties of Aspillia africana and Psidium guajava are better enhanced with their synergistic potential to the tested microorganisms. 

Hygienic quality of ewes' milk cheeses manufactured with artisan-produced lamb rennet pastes

2007 ◽  
Vol 74 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Pilar F Gil ◽  
Socorro Conde ◽  
Marta Albisu ◽  
Francisco J Pérez-Elortondo ◽  
Iñaki Etayo ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Analysis Of Variance ◽  
Basque Country ◽  
Raw Milk ◽  
Point Of View ◽  
The Other ◽  
Ripening Period ◽  
Hygienic Quality ◽  
Microbiological Analyses

The use of artisan-produced lamb rennet pastes, but not any of the other commercial animal rennets, imparts a characteristic flavour to the cheese, so most Mediterranean ewes' milk cheeses are coagulated with this kind of rennet paste. In contrast to the advantages of using lamb or kid rennet pastes from the sensory point of view, questions are still raised as to their hygienic quality. The goal was to examine the microbiological and enzymic quality of lamb rennet pastes prepared by cheese manufacturers for their own use, and evaluate the hygienic quality of raw sheeps' milk cheeses made with them, using Idiazabal cheese as a model. Lamb rennet pastes prepared by artisan cheese makers from the Basque region of Spain (27), and Italy (8) were evaluated. For cheese making experiments 5 different lamb rennet pastes were selected among the 27 samples from the Basque Country region of Spain. Microbiological analyses were carried out on samples from rennet pastes, rennet extracts, milks and cheeses during ripening. Enzymic activities studied in rennet paste were: total coagulating strength and lipase. Analysis of variance and Student's t-tests was performed. The results show that the artisan-produced rennet pastes contain high levels of a variety of microorganisms. After 60 ripening days, which is the minimum ripening period required for Idiazabal cheese prior to its commercialization, no Eschericia coli, Clostridium, Salmonella spp. or Listeria monocytogenes were detected, and levels for the rest of the microorganisms were below the limits of the European legislative standards for cheese manufactured with raw milk. We can conclude that the use of artisan-produced lamb rennet pastes of questionable hygienic quality for the manufacture of raw milk hard cheeses yields products of good hygienic quality.

scholarly journals Indigenous Knowledge Affords Antimicrobial Plants for the Management of Infections

2021 ◽  
pp. 12-21
Author(s):  
John Alake ◽  
Samuel A. Akwetey ◽  
Wisdom Ahlidja ◽  
Francis A. Armah ◽  
Isaac Kingsley Amponsah
Keyword(s):  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Dilution Method ◽  
Biomedical Sciences ◽  
Cape Coast ◽  
Disk Diffusion Method ◽  
Plant Materials

Aim: The research was carried out to ascertain the antimicrobial effect of the plants Omphalocarpum ahia, Homalium letestui, and Coelocaryon oxycarpum, which are used locally to treat some infectious diseases. Place and Duration of Study: Department of Biomedical Sciences, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana and Department of Herbal Medicine, KNUST, Ghana from June to August 2014. Method: The stem barks of the plants were extracted with 70 % methanol and successively partitioned with petroleum ether, ethyl acetate, methanol to obtain three different fractions. The antimicrobial activities of the extracts and fraction against MRSA, S. typhi, E. coli, S. pneumoniae, P. aeruginosa, E. faecalis, and S. aureus were determined using the disk diffusion method and the minimum inhibition concentration by the serial microplate dilution method with 0.2 mg/ml p-iodonitrotetrazolium as growth indicator whereas gentamycin was used as the positive control. Phytochemical tests on the plant materials were done according to standard methods. Result:  All the fractions of each plant had activity against some of the bacteria. Ethyl acetate (EA) and hydro-methanolic (CE) extracts of Coelocaryon oxycarpum exhibited activity against all selected bacteria with MIC ranging from 0.625-5 mg/ml for CE and 0.3125-5 mg/ml for EA. Hydro-methanolic (CE) extracts of Omphalocarpum ahia also exhibited antibacterial activity against all the selected bacteria. Conclusion: The current research showed that Omphalocarpum ahia and Coelocaryon oxycarpum have considerable antimicrobial activity against all the strains used in the study. Local knowledge may afford lead materials for the development of novel antimicrobial agents.

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