Wheat Sprout Extract Stimulates Cell Proliferation via Phosphorylation of ERK and Akt in Human Dermal Papilla Cells

Yakhak Hoeji ◽  
2017 ◽  
Vol 61 (3) ◽  
pp. 158-163
Author(s):  
Seol-a Park ◽  
◽  
Myoung-hee In ◽  
Won-hong Woo ◽  
Yeun-ja Mun ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Wheat Sprout

scholarly journals Extracellular Signal-Regulated Kinase Mediates Ebastine-Induced Human Follicle Dermal Papilla Cell Proliferation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Fu-Ming Tsai ◽  
Chao-Hsu Li ◽  
Lu-Kai Wang ◽  
Mao-Liang Chen ◽  
Ming-Cheng Lee ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Allergic Inflammation ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Expression Levels ◽  
Extracellular Signal Regulated Kinase ◽  
Hair Regrowth ◽  
Extracellular Signal ◽  
Elevated Expression ◽  
H1 Receptor Antagonist

Ebastine is a second-generation histamine H1 receptor antagonist that is used to attenuate allergic inflammation. Ebastine has also shown to affect hair loss; however, the immunoregulatory effect of ebastine cannot completely exclude the possibility of spontaneous hair regrowth in ebastine-treated mice. In this study, we examined the effects of ebastine on the growth of human follicle dermal papilla cells (HFDPC) using a WST-1 cell proliferation assay and a bromodeoxyuridine incorporation assay. Ebastine was shown to significantly increase the proliferation of HFDPC. The expression levels of cell-cycle regulatory proteins and an antiapoptotic protein were increased in ebastine-treated HFDPC. Furthermore, elevated expression levels of phospho-AKT and phospho-p44/42 extracellular signal-regulated kinase (ERK) were observed in ebastine-treated HFDPC. Ebastine-mediated HFDPC growth was completely reversed by blocking ERK kinase. The results from our present study suggest that the regulation of HFDPC proliferation by ebastine might be directly involved in hair regrowth through the ERK signaling pathway.

scholarly journals miR-140-5p in Small Extracellular Vesicles From Human Papilla Cells Stimulates Hair Growth by Promoting Proliferation of Outer Root Sheath and Hair Matrix Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Yuxin Chen ◽  
Junfei Huang ◽  
Zhen Liu ◽  
Ruosi Chen ◽  
Danlan Fu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Extracellular Vesicles ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Luciferase Reporter ◽  
Outer Root Sheath ◽  
Dermal Papilla Cells ◽  
Root Sheath ◽  
Hair Matrix ◽  
Low Passage

The application of dermal papilla cells to hair follicle (HF) regeneration has attracted a great deal of attention. However, cultured dermal papilla cells (DPCs) tend to lose their capacity to induce hair growth during passage, restricting their usefulness. Accumulating evidence indicates that DPCs regulate HF growth mainly through their unique paracrine properties, raising the possibility of therapies based on extracellular vesicles (EVs). In this study, we explored the effects of EVs from high- and low-passage human scalp follicle dermal papilla cells (DP-EVs) on activation of hair growth, and investigated the underlying mechanism. DP-EVs were isolated by ultracentrifugation and cultured with human scalp follicles, hair matrix cells (MxCs), and outer root sheath cells (ORSCs), and we found low-passage DP-EVs accelerated HF elongation and cell proliferation activation. High-throughput miRNA sequencing and bioinformatics analysis identified 100 miRNAs that were differentially expressed between low- (P3) and high- (P8) passage DP-EVs. GO and KEGG pathway analysis of 1803 overlapping target genes revealed significant enrichment in the BMP/TGF-β signaling pathways. BMP2 was identified as a hub of the overlapping genes. miR-140-5p, which was highly enriched in low-passage DP-EVs, was identified as a potential regulator of BMP2. Direct repression of BMP2 by miR-140-5p was confirmed by dual-luciferase reporter assay. Moreover, overexpression and inhibition of miR-140-5p in DP-EVs suppressed and increased expression of BMP signaling components, respectively, indicating that this miRNA plays a critical role in hair growth and cell proliferation. DP-EVs transport miR-140-5p from DPCs to epithelial cells, where it downregulates BMP2. Therefore, DPC-derived vesicular miR-140-5p represents a therapeutic target for alopecia.

Increase in dermal papilla cells by proliferation during development of the primary wool follicle

1992 ◽  
Vol 43 (4) ◽  
pp. 843 ◽  
Author(s):  
DL Adelson ◽  
BA Kelley ◽  
BN Nagorcka
Keyword(s):  
Cell Proliferation ◽  
Transforming Growth Factor ◽  
Current Knowledge ◽  
Diffusion Theory ◽  
Reaction Diffusion ◽  
Growth Period ◽  
Dermal Papilla ◽  
Cell Number ◽  
Dermal Papilla Cells ◽  
Developmental Modulation

The growth of the demand condensate and dermal papilla of developing primary wool follicles was investigated by counting cells in paraffin of foetal sheep skin. The number of cells in the dermal condensate of prepapilla was found to increase approximately two-fold between follicle initiation and maturation (stages F1 and F8). This increase was not continuous but was interrupted by a no growth period from stages F2a to F6. The proportion of dividing dermal condensate cells, determined by the BrdU/Anti-BrdU technique, was shown to peak during periods of cell number increase. In addition, we were able to demonstrate that the increase in cell number described above could be accounted for entirely by cell proliferation, with no contribution from migration. The developmental modulation of dermal condensate cell proliferation is discussed in relation to both the Reaction-Diffusion theory of hair follicle initiation and morphogenesis and our current knowledge of the transforming growth factor-G superfamily of proteins.

scholarly journals Induction of Cell Proliferation and Cell Death in Human Follicle Dermal Papilla Cells by Zinc Chloride

2019 ◽  
Vol 81 (4) ◽  
Author(s):  
F. M Tsai ◽  
L. K Wang ◽  
M. L Chen ◽  
M. C Lee ◽  
Y. C Lin ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Zinc Chloride ◽  
Dermal Papilla ◽  
Dermal Papilla Cells

scholarly journals Proliferation Effects on Hair Growth of Compounds Isolated from the Bark of Dalbergia oliveri

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Seon Ju Park ◽  
Nguyen Xuan Nhiem ◽  
Bui Huu Tai ◽  
Hoang Le Tuan Anh ◽  
Seok Hyun Oh ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Hair Growth ◽  
Spectroscopic Data ◽  
Dermal Papilla ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Proliferation Assay ◽  
Dermal Papilla Cells ◽  
Cell Proliferation Assay ◽  
A Cell

One new isoflavane, 7,4′-dihydroxy-isoflavanquinone (1), together with ten known compounds (2–11) were isolated from the bark of Dalbergia oliveri Prain. The structures of compounds were determined on the basis of extensive spectroscopic methods, including 1D and 2D NMR and CD spectroscopic data. Using a cell proliferation assay, the isolated compounds were evaluated for their proliferation effects on hair growth. (3 R)-5’-Methoxyvestitol (2) and (6a R,11a R)-3,8-dihydroxy-9-methoxypterocarpan (10) significantly increased the proliferation of immortalized dermal papilla cells (iDPC).

scholarly journals Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth

2021 ◽  
Vol 9 ◽  
Author(s):  
Yufan Zhang ◽  
Junfei Huang ◽  
Danlan Fu ◽  
Zhen Liu ◽  
Hailin Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
3D Model ◽  
Caspase 3 ◽  
Dermal Papilla ◽  
Inhibitory Effect ◽  
Follicle Growth ◽  
Hub Genes ◽  
Dermal Papilla Cells ◽  
Hair Follicle Growth ◽  
2D And 3D

Dermal papillae are a target of androgen action in patients with androgenic alopecia, where androgen acts on the epidermis of hair follicles in a paracrine manner. To mimic the complexity of the dermal papilla microenvironment, a better culture model of human dermal papilla cells (DPCs) is needed. Therefore, we evaluated the inhibitory effect of dihydrotestosterone (DHT)-treated two-dimensional (2D)- and 3D-cultured DPCs on hair follicle growth. 2D- and 3D-cultured DPC proliferation was inhibited after co-culturing with outer root sheath (ORS) cells under DHT treatment. Moreover, gene expression levels of β-catenin and neural cell adhesion molecules were significantly decreased and those of cleaved caspase-3 significantly increased in 2D- and 3D-cultured DPCs with increasing DHT concentrations. ORS cell proliferation also significantly increased after co-culturing in the control-3D model compared with the control-2D model. Ki67 downregulation and cleaved caspase-3 upregulation in DHT-treated 2D and 3D groups significantly inhibited ORS cell proliferation. Sequencing showed an increase in the expression of genes related to extracellular matrix synthesis in the 3D model group. Additionally, the top 10 hub genes were identified, and the expression of nine chemokine-related genes in DHT-treated DPCs was found to be significantly increased. We also identified the interactions between transcription factor (TF) genes and microRNAs (miRNAs) with hub genes and the TF–miRNA coregulatory network. Overall, the findings indicate that 3D-cultured DPCs are more representative of in vivo conditions than 2D-cultured DPCs and contribute to our understanding of the molecular mechanisms underlying androgen-induced alopecia.

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