Antioxidant activity by DPPH assay: in vitro protocol v1 (protocols.io.btbpnimn)

The reaction of indoline with a substituted benzoyl chloride in the presence of K2CO3 in THF gave compound 4. Compound 4 was subjected to chlorosulphonation to obtain compound 5. Condensation of aromatic amines with compound 5 led to the synthesis of indoline derivatives 6(a-f). Similarly, 5-nitroindoline was treated with a substituted benzoyl chloride to obtain the nitro compound 9, which was reduced using stannous chloride and reacted further with aromatic sulphonyl chloride to obtain the indoline derivatives 11(a-e). These compounds were tested for antibacterial, anti-tuberculosis and antifungal activity. Some of them showed very good activity against some gram-positive and gram negative bacteria, fungal strains and also Mycobacterium tuberculosis. All of the synthesized compounds were subjected to antioxidant activity testing using the in vitro DPPH assay and most of them showed very good activity.

Download Full-text

Antioxidant Activity of Plant Extracts from Colombian Coffee-Growing Eco-Region

Revista Facultad de Ciencias Básicas ◽

10.18359/rfcb.2755 ◽

2017 ◽

Vol 13 (1) ◽

pp. 56-59

Author(s):

Aura M. Blandón ◽

Oscar M. Mosquera ◽

Antônio E. G. Sant’ana ◽

Aldenir F. Dos Santos ◽

Luana L. S. Pires

Keyword(s):

Antioxidant Activity ◽

Plant Species ◽

Plant Extracts ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Potential ◽

Dpph Assay ◽

Methanolic Extracts ◽

In Vitro Antioxidant Activity

The present study describes the in vitro antioxidant activity of methanol extracts of 34 plant species collected in the Colombian coffee-growing eco-region belonging to Euphorbiaceae, Piperaceae and Solanaceae families. The antioxidant properties of extracts were evaluated by determining radical scavenging power measured with a DPPH assay. The methanolic extracts of Hyeronimia antioquiensis, Mabea montana, and Alchornea grandis species (Euphorbiaceae), presents EC50 values equal to 0.686, 12.35, and 13.01 µg/mL, respectively, showing high antioxidant potential.

Download Full-text

Influence of Honey on the Suppression of Human Low Density Lipoprotein (LDL) Peroxidation (In Vitro)

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem071 ◽

2009 ◽

Vol 6 (1) ◽

pp. 113-121 ◽

Cited By ~ 28

Author(s):

Ahmed G. Hegazi ◽

Faten K. Abd El-Hady

Keyword(s):

Antioxidant Activity ◽

Hplc Analysis ◽

Low Density Lipoprotein ◽

Radical Scavenging ◽

Density Lipoprotein ◽

Free Radical Scavenging ◽

Low Density ◽

Dpph Assay ◽

Free Radical Scavenging Assay

The antioxidant activity of four honey samples from different floral sources (Acacia, Coriander, Sider and Palm) were evaluated with three different assays; DPPH free radical scavenging assay, superoxide anion generated in xanthine–xanthine oxidase (XOD) system and low density lipoprotein (LDL) peroxidation assay. The dark Palm and Sider honeys had the highest antioxidant activity in the DPPH assay. But all the honey samples exhibited more or less the same highly significant antioxidant activity within the concentration of 1mg honey/1 ml in XOD system and LDL peroxidation assays. The chemical composition of these samples was investigated by GC/MS and HPLC analysis, 11 compounds being new to honey. The GC/MS revealed the presence of 90 compounds, mainly aliphatic acids (37 compounds), which represent 54.73, 8.72, 22.87 and 64.10% and phenolic acids (15 compound) 2.3, 1.02, 2.07 and 11.68% for Acacia, Coriander, Sider and Palm honeys. In HPLC analysis, 19 flavonoids were identified. Coriander and Sider honeys were characterized by the presence of large amounts of flavonoids.

Download Full-text

Lipid-based nanocarriers to enhance skin permeation and antioxidant activity of Centella asiatica extract

MATEC Web of Conferences ◽

10.1051/matecconf/201819201016 ◽

2018 ◽

Vol 192 ◽

pp. 01016

Author(s):

Boonnada Pamornpathomkul ◽

Worranan Rangsimawong ◽

Theerasak Rojanarata ◽

Praneet Opanasopit ◽

Chuleerath Chaiyodsilp ◽

...

Keyword(s):

Antioxidant Activity ◽

Skin Permeation ◽

Centella Asiatica ◽

Entrapment Efficiency ◽

Dpph Assay ◽

Significant Difference ◽

In Vitro Skin Permeation ◽

Permeation Studies ◽

Solution Formulation

The purpose of this study was to evaluate the use of different formulations, including solution, gel, liposome and niosome for in vitro skin permeation and antioxidant activity of Centella asiatica (CA) extract. The liposomes and niosomes loaded with CA were characterized to observe the physicochemical properties i.e., particle size, zeta potential, percentage of entrapment efficiency (%EE) and percentage of loading efficiency (%LE). In vitro skin permeation studies revealed that liposome formulations had a superior enhancing effect on skin permeation compared to niosome, gel and solution formulation. Upon applied niosome formulations for the delivery of CA extract at 24 hours (h), the antioxidant activity was higher than liposome, gel and solution formulation, as evidenced by the increased in percent inhibition using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. However, there was no significant difference in antioxidant activity between niosome and liposome formulations. Accordingly, both the liposome and noisome formulations are promising approaches for transdermal delivery of CA extract for promoting successful antioxidant activity.

Download Full-text

Evaluation of antioxidant activity of ormeloxifene: in vitro study

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20174385 ◽

2017 ◽

Vol 6 (10) ◽

pp. 2507

Author(s):

Vinay M. ◽

Seethalakshmi S. ◽

Vijay Kumar

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

No Synthase ◽

Free Radical Scavenging Activity ◽

Scavenging Activity ◽

Dpph Assay

Background: Ormeloxifene (Centchroman) is a Selective Estrogen Receptor Modulator (SERM) which acts as estrogen antagonist and having anti progestogenic activity also. It is being used in the management of dysfunctional uterine bleeding and as nonhormonal oral contraceptive. It is also being investigated for the indications such as osteoporosis, breast and endometrial carcinoma. In this study, we have evaluated the Antioxidant potential of drug by using DPPH and NO synthase Assay. It was found that ormeloxifene has significant antioxidant activity which could be cause for its use in various gynaecological and other conditions.Methods: In this study, we have demonstrated in vitro antioxidant activity of ormeloxifene. DPPH and NO synthase assay tests were done for different concentrations of ormeloxifene.Results: In our study, it showed that the free radical scavenging activity of ormeloxifene was less in lower concentration and increased in the higher concentration in DPPH assay. The free radical scavenging activity of drug ormeloxifene was 22% at 100µg/ml and 27% for the concentrations of 1000µg/ml in DPPH assay. No scavenging activity was 3% at 100µg/ml and 11% at 1000µg/ml.Conclusions: The invitro antioxidant analysis of ormeloxifene, was proved to be a potent antioxidant.

Download Full-text

Anti-malarial activity of the leaf latex of Aloe weloensis (Aloaceae) against plasmodium parasites

10.21203/rs.3.rs-37849/v2 ◽

2021 ◽

Author(s):

Gedefaw Getnet Amare ◽

Amsalu Degu ◽

Zemene Demelash Kifle

Keyword(s):

Antioxidant Activity ◽

Folk Medicine ◽

Inhibition Activity ◽

Survival Times ◽

Dpph Assay ◽

Curative Effect ◽

Parasitemia Level ◽

Dose Dependent

Abstract Background: Lack of available vaccines and emerging resistance on the anti-malarial drug have provided the necessity to find noble plant--based anti-malarial drugs. The leaf latex Aloe weloensis has been used in folk medicine against malarial and other human ailments in Ethiopia. Hence, the present study aimed to investigate the anti-malarial activity of the leaf latex of A. weloensis against Plasmodium parasites to validate its traditional claim.Methods: The leaf latex of A. weloensis was evaluated in vitro anti-malarial activity against 3D7 strain of Plasmodium falciparum. The prophylactic and curative models were employed to determine in vivo anti-malarial activity of the latex against P. berghei infected mice, and antioxidant activity of the leaf latex of A. weloensis was assessed in DPPH assay.Results: The leaf latex of Aloe weloensis endowed with free radical inhibition activity (IC50 = 10.25 μg/ml). The latex of A. weloensis leaf was demonstrated inhibitory activity against 3D7 strain of P. falciparum (IC50 = 9.14 μg/ml). The prophylactic and curative effect of the latex was found to be dose-dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in prophylactic test compared to the control. Parasitemia level of the mice treated with 200, 400, and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58%, and 74% respectively in the curative test. The leaf latex significantly (p<0.01) improved mean survival times, packed cell volume , rectal temperature, and bodyweight of P. berghei infected mice.Conclusion: The result was confirmed the anti-malarial activity of the leaf latex of Aloe weloensis at various doses which corroborates the traditional uses of the plant.

Download Full-text

Synthesis, in vitro Antioxidant Activity, and Toxicity Evaluation of Hydrazone Derivatives Naphthalene-1-ylmethylene hydrazine

Journal of Physics Conference Series ◽

10.1088/1742-6596/2049/1/012050 ◽

2021 ◽

Vol 2049 (1) ◽

pp. 012050

Author(s):

Jasril ◽

E Juwiyatri ◽

S N Fauza ◽

N Afriana

Keyword(s):

Antioxidant Activity ◽

Hydrazine Hydrate ◽

Basic Structure ◽

Artemia Salina ◽

Dpph Assay ◽

Azomethine Group ◽

H Nmr ◽

Physical And Chemical ◽

In Vitro Antioxidant Activity

Abstract Hydrazone is a versatile organic compound that has a basic structure (-NHN=CH-) called the azomethine group. This structure is responsible for the physical and chemical of hydrazone, which makes this compound has variety bioactivities such as antioxidant, antitumor, and anticancer. In this work, two hydrazone derivatives from 1-naphthaldehyde and hydrazine (phenylhydrazine/hydrazine hydrate) have been synthesized under microwave irradiation. Their antioxidant activity and toxicity were evaluated by DPPH and BSLT method, respectively. Structures of the synthesized compounds were confirmed based on spectroscopic data included UV, FTIR, HRMS, and 1H-NMR. Based on the DPPH assay, hydrazone from phenylhydrazine has strong antioxidant (IC50 28.90 μg/mL) but inactive antioxidant for hydrazine hydrate (IC50 >1000 μg/mL). However, both compounds have a high toxicity effect on Artemia Salina Leach with each LC50 1.45 and 47.20 μg/mL, hence they have the potential to be developed into anticancer drugs.

Download Full-text

Fatty Acid Composition, Antioxidant, and in vitro Anti-inflammatory Activity of Five Cold-Pressed Prunus Seed Oils, and Their Anti-biofilm Effect Against Pathogenic Bacteria

Frontiers in Nutrition ◽

10.3389/fnut.2021.775751 ◽

2021 ◽

Vol 8 ◽

Author(s):

Florinda Fratianni ◽

Antonio d'Acierno ◽

Maria Neve Ombra ◽

Giuseppe Amato ◽

Vincenzo De Feo ◽

...

Keyword(s):

Antioxidant Activity ◽

Oleic Acid ◽

Fatty Acid ◽

Inflammatory Activity ◽

Seed Oils ◽

Black Cherry ◽

Dpph Assay ◽

Anti Inflammatory ◽

Cold Pressed

Background/Aim: Sweet almond (Prunus amygdalus dulcis) oil is one of the most famous cold-pressed seed oils. However, other species of Prunus can provide oils with healthy properties. We analyzed the fatty acid (FA) composition, as well as the antioxidant, the in vitro anti-inflammatory properties, and the antibiofilm activity of five commercial vegetable cold-pressed seed oils of apricot, peach, plum, cherry, and black cherry.Methods: Gas Chromatography-Mass Spectrometry was performed for the analysis of FAs The antioxidant property of the oils was carried using different tests [2, 2-diphenyl-1-picrylhydrazyl (DPPH assay)], Ferric Reducing Antioxidant Power (FRAP), and the 2, 20 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS·+). The denaturation assay performed on bovine serum albumin (BSA) was used to evaluate the in vitro anti-inflammatory activity. The anti-biofilm activity was assessed using five pathogenic strains, namely, Acinetobacter baumannii, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus, through the crystal violet test and the 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT), used to evaluate the metabolism of the microbial cells present within the biofilm.Results: Oleic acid and linoleic acids were the most abundant FAs. Black cherry seed oil exhibited the best antioxidant activity, but in general, the amount of oil needed to inhibit the activity of 1 ml of DPPH assay at 50% did not exceed 10 μg. The extract concentration for the 50% inhibition of the denaturation of the protein (IC50) did not exceed 4.4 μg. Linoleic and stearic acids affected the antioxidant activity of the oils; oleic acid, linolenic, and palmitoleic acids exhibited beneficial effects in preserving the BSA denaturation, as shown by the correlation data. The oils were able to inhibit the biofilm formation of the pathogens (up to 71.40% of inhibition) as well as act against their mature biofilm, although with different strengths, with values up to 61.54%. Concurrently, they also acted on the pathogen metabolism.Conclusion: The oils represent a valuable source of some healthy FAs. They showed potential antioxidant and anti-inflammatory in vitro activity, in addition, their potential effect on the biofilm can offer important ideas for research and reflection on their use as functional foods and/or ingredients.

Download Full-text

Anti-malarial activity of the leaf latex of Aloe weloensis (Aloaceae) against plasmodium parasites

10.21203/rs.3.rs-37849/v3 ◽

2021 ◽

Author(s):

Gedefaw Getnet Amare ◽

Amsalu Degu ◽

Zemene Demelash Kifle

Keyword(s):

Antioxidant Activity ◽

Folk Medicine ◽

Inhibition Activity ◽

Survival Times ◽

Dpph Assay ◽

Curative Effect ◽

Parasitemia Level ◽

Dose Dependent

Abstract Lack of available vaccines and emerging resistance on the anti-malarial drug have provided the necessity to find noble plant--based anti-malarial drugs. The leaf latex Aloe weloensis has been used in folk medicine against malarial and other human ailments in Ethiopia. Hence, the present study aimed to investigate the anti-malarial activity of the leaf latex of A. weloensis against Plasmodium parasites to validate its traditional claim. Methods: The leaf latex of A. weloensis was evaluated in vitro anti-malarial activity against 3D7 strain of Plasmodium falciparum. The prophylactic and curative models were employed to determine in vivo anti-malarial activity of the latex against P. berghei infected mice, and antioxidant activity of the leaf latex of A. weloensis was assessed in DPPH assay. Results: The leaf latex of Aloe weloensis endowed with free radical inhibition activity (IC50 = 10.25 μg/ml). The latex of A. weloensis leaf was demonstrated inhibitory activity against 3D7 strain of P. falciparum (IC50 = 9.14 μg/ml). The prophylactic and curative effect of the latex was found to be dose-dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in prophylactic test compared to the control. Parasitemia level of the mice treated with 200, 400, and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58%, and 74% respectively in the curative test. The leaf latex significantly (p<0.01) improved mean survival times, packed cell volume , rectal temperature, and bodyweight of P. berghei infected mice. Conclusion: The result was confirmed the anti-malarial activity of the leaf latex of Aloe weloensis at various doses which corroborates the traditional uses of the plant.

Download Full-text

Olive Mill Waste Extracts: Polyphenols Content, Antioxidant, and Antimicrobial Activities

Advances in Pharmacological Sciences ◽

10.1155/2015/714138 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 23

Author(s):

Inass Leouifoudi ◽

Hicham Harnafi ◽

Abdelmajid Zyad

Keyword(s):

Antioxidant Activity ◽

Antimicrobial Activities ◽

Total Phenol Content ◽

Dpph Assay ◽

Liquid Chromatograph ◽

Plain Area ◽

Diffusion Assay ◽

Gel Diffusion ◽

Olive Mill

Natural polyphenols extracts have been usually associated with great bioactive properties. In this work, we investigatedin vitroantioxidant and antimicrobial potential of the phenolic olive mill wastewater extracts (OWWE) and the olive cake extracts (OCE). Using the Folin Ciocalteux method, OWWE contained higher total phenol content compared to OCE (8.90±0.728 g/L versus0.95±0.017 mg/g). The phenolic compounds identification was carried out with a performance liquid chromatograph coupled to tandem mass spectrometry equipment (HPLC-ESI-MS). With this method, a list of polyphenols from OWWE and OCE was obtained. The antioxidant activity was measured in aqueous (DPPH) and emulsion (BCBT) systems. Using the DPPH assay, the results show that OWWE was more active than OCE and interestingly the extracts originating from mountainous areas were more active than those produced from plain areas (EC50=12.1±5.6 μg/mL;EC50=157.7±34.9 μg/mL, resp.). However, when the antioxidant activity was reversed in the BCBT, OCE produced from plain area was more potent than mountainous OCE. Testing by the gel diffusion assay, all the tested extracts have showed significant spectrum antibacterial activity againstStaphylococcus aureus, whereas the biophenols extracts showed more limited activity againstEscherichia coliandStreptococcus faecalis.

Download Full-text