PSI Open Fluor CAM script for measuring qE component of NPQ in Chlorella vulgaris v1

2021 ◽  
Author(s):  
Andrei Herdean
Keyword(s):  
Dark Adaptation ◽  
Red Light ◽  
Fluorescence Measurement ◽  
Actinic Light ◽  
Led Array ◽  
Red Led ◽  
Simple Protocol ◽  
Measured Sample ◽  
Array Camera ◽  
Led Arrays

This is a simple protocol that consists of 1) 10 minutes preillumination with far red light 2) 5 minutes of illumination with actinic light 3) 5 minutes of dark adaptation with far red light qE is calculated as the differe between NPQ_Lss and NPQ_D5 qE=NPQ_Lss-NPQ_D5 qI=NPQ_D5 Protocol to be used with FluorCAM 7.0 on a PSI Open FC 800-O/1010-S. Act 2 - are the white light LED arrays ADD2 - is the far red LED array Camera is placed at ~20 cm above the measured sample. Light intensity uniformity across the 96 well plate was measured according to manufacturer instructions. !Important - protocol only works under weak far red light. Intense far red will interfere with the fluorescence measurement.

scholarly journals Spectral Quality Affects Disease Development of Three Pathogens on Hydroponically Grown Plants

HortScience ◽  
1997 ◽  
Vol 32 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Andrew C. Schuerger ◽  
Christopher S. Brown
Keyword(s):  
Light Source ◽  
Red Light ◽  
Peak Height ◽  
Tomato Mosaic Virus ◽  
Light Sources ◽  
Disease Development ◽  
Spectral Quality ◽  
Disease Symptoms ◽  
Led Array ◽  
Led Arrays

Plants were grown under light-emitting diode (LED) arrays with various spectra to determine the effects of light quality on the development of diseases caused by tomato mosaic virus (ToMV) on pepper (Capsicum annuum L.), powdery mildew [Sphaerotheca fuliginea (Schlectend:Fr.) Pollaci] on cucumber (Cucumis sativus L.), and bacterial wilt (Pseudomonas solanacearum Smith) on tomato (Lycopersicon esculentum Mill.). One LED (660) array supplied 99% red light at 660 nm (25 nm bandwidth at half-peak height) and 1% far-red light between 700 to 800 nm. A second LED (660/735) array supplied 83% red light at 660 nm and 17% far-red light at 735 nm (25 nm bandwidth at half-peak height). A third LED (660/BF) array supplied 98% red light at 660 nm, 1% blue light (BF) between 350 to 550 nm, and 1% far-red light between 700 to 800 nm. Control plants were grown under broad-spectrum metal halide (MH) lamps. Plants were grown at a mean photon flux (300 to 800 nm) of 330 μmol·m-2·s-1 under a 12-h day/night photoperiod. Spectral quality affected each pathosystem differently. In the ToMV/pepper pathosystem, disease symptoms developed slower and were less severe in plants grown under light sources that contained blue and UV-A wavelengths (MH and 660/BF treatments) compared to plants grown under light sources that lacked blue and UV-A wavelengths (660 and 660/735 LED arrays). In contrast, the number of colonies per leaf was highest and the mean colony diameters of S. fuliginea on cucumber plants were largest on leaves grown under the MH lamp (highest amount of blue and UV-A light) and least on leaves grown under the 660 LED array (no blue or UV-A light). The addition of far-red irradiation to the primary light source in the 660/735 LED array increased the colony counts per leaf in the S. fuliginea/ cucumber pathosystem compared to the red-only (660) LED array. In the P. solanacearum/ tomato pathosystem, disease symptoms were less severe in plants grown under the 660 LED array, but the effects of spectral quality on disease development when other wavelengths were included in the light source (MH-, 660/BF-, and 660/735-grown plants) were equivocal. These results demonstrate that spectral quality may be useful as a component of an integrated pest management program for future space-based controlled ecological life support systems.

scholarly journals Critical Photoperiod and Optimal Quality of Night Interruption Light for Runner Induction in June-Bearing Strawberries

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1996
Author(s):  
Yali Li ◽  
Jie Xiao ◽  
Jiangtao Hu ◽  
Byoung Ryong Jeong
Keyword(s):  
Red Light ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Light Emitting ◽  
Red Led ◽  
Critical Photoperiod ◽  
Closed Walk ◽  
White Light Emitting Diodes ◽  
Optimal Quality

The optimal photoperiod and light quality for runner induction in strawberries ‘Sulhyang’ and ‘Maehyang’ were investigated. Two experiments were carried out in a semi-closed walk-in growth chamber with 25/15 °C day/night temperatures and a light intensity of 250 μmol·m–2·s–1photosynthetic photon flux density (PPFD) provided from white light-emitting diodes (LEDs). In the first experiment, plants were treated with a photoperiod of either 12, 14, 16, 18, 20, or 22 h In the second experiment, a total of 4 h of night interruption (NI) light at an intensity of 70 μmol·m–2·s–1PPFD provided from either red, blue, green, white, or far-red LED in addition to 11 h short day (SD). The results showed that both ‘Sulhyang’ and ‘Maehyang’ produced runners when a photoperiod was longer than 16 h, and the number of runners induced positively correlated with the length of photoperiod. However, the plant growth, contents of chlorophyll, sugar and starch, and Fv/Fo decreased in a 22 h photoperiod. All qualities of the NI light, especially red light, significantly increased the number of runners and daughter plants induced per plant as compared with those in the SD treatment in both cultivars. In a conclusion, a photoperiod between 16 and 20 h and NI light, especially red NI light, can be used for quality runner induction in both ‘Sulhyang’ and ‘Maehyang’.

scholarly journals Red LED Light Acts on the Mitochondrial Electron Chain of Donkey Sperm and Its Effects Depend on the Time of Exposure to Light

2020 ◽  
Vol 8 ◽  
Author(s):  
Jaime Catalán ◽  
Marion Papas ◽  
Lina Trujillo-Rojas ◽  
Olga Blanco-Prieto ◽  
Sebastián Bonilla-Correal ◽  
...  
Keyword(s):  
Red Light ◽  
Specific Inhibitor ◽  
Motile Sperm ◽  
Light Stimulation ◽  
Led Light ◽  
Mitochondrial Atp Synthase ◽  
Red Led ◽  
Oligomycin A ◽  
Rate Of Oxygen Consumption ◽  
Stimulation Of

This work aimed to investigate how stimulation of donkey sperm with red LED light affects mitochondrial function. For this purpose, freshly diluted donkey semen was stimulated with red light for 1, 5, and 10 min, in the presence or absence of oligomycin A (Omy A), a specific inhibitor of mitochondrial ATP synthase, or FCCP, a specific disruptor of mitochondrial electron chain. The results obtained in the present study indicated that the effects of red LED light on fresh donkey sperm function are related to changes in mitochondria function. In effect, irradiation of donkey sperm resulted in an increase in mitochondrial membrane potential (MMP), the activity of cytochrome C oxidase and the rate of oxygen consumption. In addition, in the absence of oligomycin A and FCCP, light-stimulation augmented the average path velocity (VAP) and modified the structure of motile sperm subpopulations, increasing the fastest and most linear subpopulation. In contrast, the presence of either Omy A or FCCP abolished the aforementioned effects. Interestingly, our results also showed that the effects of red light depend on the exposure time applied, as indicated by the observed differences between irradiation protocols. In conclusion, our results suggest that exposing fresh donkey sperm to red light modulates the function of their mitochondria through affecting the activity of the electron chain. However, the extent of this effect depends on the irradiation pattern and does not exclude the existence of other mechanisms, such as those related to thermotaxis.

Fundamentals and Applications of Red Light-Emitting Diodes (LEDs) In Vitro Plant Growth on Tomato Lycopersicon esculentum Mill

2019 ◽  
Vol 805 ◽  
pp. 141-145
Author(s):  
Nguyen Phuc Thien
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Significant Influence ◽  
Growth And Development ◽  
Red Light ◽  
Light Emitting ◽  
Red Led ◽  
In Vitro Plant ◽  
Normal Light

The aim of these studies was mainly to investigate the effects of monochromatic LEDs applied singly on the in vitro plant growth and morphogenesis. Various morphological and physiological parameters are considered that influence the growth and development of plants in vitro under red LED light as compared to those under normal light. Upon exposure to LED, in vitro-raised plants have shown significant improvements in growth and morphogenesis. In particular, red and blue lights, either alone or in combination, have a significant influence on plant growth. The present study gives an overview of the fundamentals of LEDs and describes their effects on in vitro plant growth and morphogenesis and their future potentials. The main objective of this study was to carry out line and combing ability of plant growth on tomato.

Syntheses, crystal structures and photoluminescence properties of Ca9Y(PO4)5(SiO4)F1.5O0.25:Ln3+ (Ln3+ = Eu3+/Tb3+/Dy3+/Sm3+) phosphors for near-UV white LEDs

RSC Advances ◽  
2016 ◽  
Vol 6 (95) ◽  
pp. 92371-92377 ◽  
Author(s):  
Man Liu ◽  
Wei Lü ◽  
Jiansheng Huo ◽  
Baiqi Shao ◽  
Yang Feng ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Red Light ◽  
Photoluminescence Properties ◽  
Light Emitting ◽  
White Leds ◽  
Red Led

We report red light-emitting Ca9Y(PO4)5(SiO4)F1.5O0.25:Eu3+/Sm3+ phosphors for the fabrication of red LED devices.

scholarly journals Structural and luminescent characteristic of Sm3+ doped magnesium sulfide borate red phosphor for white LED

2017 ◽  
Vol 13 (4) ◽  
pp. 807-811
Author(s):  
Saddiq Abubakar Dalhatu ◽  
Rosli Hussin ◽  
Karim Deraman ◽  
Bulus Ibrahim ◽  
Yamusa Abdullahi ◽  
...  
Keyword(s):  
Red Light ◽  
Spectral Studies ◽  
White Led ◽  
Excitation Spectra ◽  
Structural Units ◽  
Red Phosphor ◽  
Red Led ◽  
Raman Spectral ◽  
Ir And Raman ◽  
Magnesium Sulfide

Polycrystalline compositions based on MgO, SO3 and B2O3 have both scientific and technological importance because of their useful applications. Doping with rare earth elements not only due to a rearrangement in the structure, but also to variation in the luminescence properties. Magnesium sulfide borate doped samarium oxide (MgSBO3:Sm3+) phosphors were prepared by solid state reaction and their structural and luminescence characteristic were studied and reported. IR and Raman spectral studies have been made to explore the presence of functional groups and various structural units in the prepared Polycrystalline. The formation of SO4, BO4, BO3, B-O-B and S-O-B structural units have been investigated. The emission and excitation properties were studied. And the results show that the emission and excitation spectra of these phosphors can be excited by ultraviolet (UV) 341, 370 and 409 nm light, and emit green, yellow and red light with intense peak at 601 nm, which are nicely in accordance with the widely applied near- UV LED chip. The emission intensities of these phosphors were increased with increases doping concentration. Therefore, these phosphors are the promising luminescent material towards white red LED applications.

scholarly journals Growth and Energy Use Efficiency of Grafted Tomato Transplants as Affected by LED Light Quality and Photon Flux Density

Agriculture ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 816
Author(s):  
Jianfeng Zheng ◽  
Peidian Gan ◽  
Fang Ji ◽  
Dongxian He ◽  
Po Yang
Keyword(s):  
Energy Use ◽  
Light Quality ◽  
Red Light ◽  
Electrical Energy ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Red Led ◽  
Use Efficiency ◽  
Led Lights

This study was conducted to compare the effects of broad spectrum during the whole seedling period and photon flux density (PFD) in the healing stage on the growth and energy use efficiency of grafted tomato (Lycopersicon esculentum Mill.) transplants in a plant factory. Fluorescent lights, white LED lights, and white plus red LED lights were applied at the growth processes of grafted tomato transplants from germination of rootstock and scion to post-grafting. Three levels of PFD (50, 100, 150 μmol m−2 s−1) were set in the healing stage under each kind of light quality. The results indicated that the growth and quality of grafted tomato transplants under different broad spectrums were influenced by the ratio of red to blue light (R/B ratio) and the ratio of red to far-red light (R/FR ratio). A higher R/B ratio was beneficial to total dry matter accumulation, but excessive red light had a negative effect on the root to shoot ratio and the seedling quality index. The higher blue light and R/FR ratio suppressed stem extension synergistically. The LED lights had good abilities to promote plant compactness and leaf thickness in comparison with fluorescent lights. The plant compactness and leaf thickness increased with the increase in daily light integral in the healing stage within a range from 2.5 to 7.5 mol m−2 d−1 (PFD, 50 to 150 μmol m−2 s−1). Compared to fluorescent lights, the LED lights showed more than 110% electrical energy saving for lighting during the whole seedling period. Higher PFD in the healing stage did not significantly increase the consumption of electric power for lighting. White plus red LED lights with an R/B ratio of 1.2 and R/FR ratio of 16 were suggested to replace fluorescent lights for grafted tomato transplants production considering the high quality of transplants and electrical energy saving, and PFD in the healing stage was recommended to be set to 150 μmol m−2 s−1.

scholarly journals The Growth and Development of Sweet Basil (Ocimum basilicum) and Bush Basil (Ocimum minimum) Grown under Three Light Regimes in a Controlled Environment

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 743 ◽  
Author(s):  
Aldarkazali ◽  
Rihan ◽  
Carne ◽  
Fuller
Keyword(s):  
Plant Growth ◽  
Radiant Heat ◽  
Oil Yield ◽  
Light Sources ◽  
White Led ◽  
Light Emitting ◽  
Sweet Basil ◽  
Light Regimes ◽  
Led Array ◽  
Red Led

Light is a crucial element for plant growth and production. In areas where natural light is not sufficient for optimal plant growth and production, high pressure sodium (HPS) light sources are widely used. However, HPS lamps are considered not very electrically efficient generating high radiant heat and as a consequence, there has been a lot of interest in replacing HPS lamps with new more efficient lighting sources in the form of light-emitting diodes (LEDs). The effects of three lighting sources (White LED, Blue/Red LED and HPS) on the growth, development and on the essential oil yield and quality of sweet basil and bush basil were investigated. There was a clear advantage to the Blue/Red (452 nm and 632 nm, respectively) LED on virtually all growth and physiological parameters measured for both basil species. The HPS lighting system always performed least effectively in all comparisons. Combining increases in plant yield and increases in oil yield the Blue/Red LED array outperformed the HPS lights by a factor of approximately double, with the white LED being intermediate between these two extremes.

Photoactivation of cytochrome с oxidase activity in liver mitochondria of Japanese quail by therapeutic doses of blue and red LED irradiation

2018 ◽  
pp. 25-30
Author(s):  
А.Е. Донцов ◽  
Н.Б. Сережникова ◽  
Л.С. Погодина ◽  
Т.С. Гурьева ◽  
П.П. Зак
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Blue Light ◽  
Japanese Quail ◽  
Oxidase Activity ◽  
Red Light ◽  
Liver Mitochondria ◽  
Coturnix Japonica ◽  
Red Led ◽  
Led Irradiation

Цель исследования - выяснение влияния синего (450 нм) и красного (630 нм) светодиодного облучения различной энергетической экспозиции на активность митохондриальной цитохром с-оксидазы. Методики. Объектом исследования служили митохондрии, изолированные из печени японского перепела Coturnix japonica . Цитохром с-оксидазную активность измеряли по скорости окисления восстановленного тетраметил-р-фенилендиамина в присутствии ротенона. Результаты. Показано, что облучение митохондрий синим светом с энергетическими экспозициями, не превышающими 4 Дж/см вызывает примерно 5-15-процентную стимуляцию активности цитохром с-оксидазы, а дозы больше 5 Дж/см приводят к ингибированию активности фермента. Облучение митохондрий красным светом также оказывает стимулирующее действие (10-20%) на цитохром с-оксидазную активность по сравнению с необлученными образцами. Заключение. Предполагается, что низкодозовое облучение синим светом может иметь сходное с красным светом терапевтическое действие при фотобиомодуляции. The aim was to study the effect of blue (450 nm) and red (630 nm) LED irradiation with different energy exposures on activity of mitochondrial cytochrome c oxidase. Methods. The study was performed on mitochondria isolated from the liver of Japanese quail Coturnix japonica . Cytochrome c oxidase activity was measured by the rate of oxidation of reduced tetramethyl-p-phenylenediamine in the presence of rotenone. Results. Irradiation of mitochondria with blue light at doses not exceeding 4 J/cm caused approximately 5-15% stimulation of cytochrome c oxidase activity while doses higher than 5 J/cm led to inhibition of this enzyme. Irradiation of mitochondria with red light also exerted a slight stimulating effect (10-20%) on cytochrome c oxidase activity compared to unirradiated samples. Conclusion. The study suggested that low-dose irradiation with blue light may produce a therapeutic effect similar to red light in photobiomodulation.

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