Fundamentals and Applications of Red Light-Emitting Diodes (LEDs) In Vitro Plant Growth on Tomato Lycopersicon esculentum Mill

2019 ◽  
Vol 805 ◽  
pp. 141-145
Author(s):  
Nguyen Phuc Thien
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Growth ◽  
Significant Influence ◽  
Growth And Development ◽  
Red Light ◽  
Light Emitting ◽  
Red Led ◽  
In Vitro Plant ◽  
Normal Light

The aim of these studies was mainly to investigate the effects of monochromatic LEDs applied singly on the in vitro plant growth and morphogenesis. Various morphological and physiological parameters are considered that influence the growth and development of plants in vitro under red LED light as compared to those under normal light. Upon exposure to LED, in vitro-raised plants have shown significant improvements in growth and morphogenesis. In particular, red and blue lights, either alone or in combination, have a significant influence on plant growth. The present study gives an overview of the fundamentals of LEDs and describes their effects on in vitro plant growth and morphogenesis and their future potentials. The main objective of this study was to carry out line and combing ability of plant growth on tomato.

Review: role of carbon sources for in vitro plant growth and development

2012 ◽  
Vol 40 (4) ◽  
pp. 2837-2849 ◽  
Author(s):  
Mehwish Yaseen ◽  
Touqeer Ahmad ◽  
Gaurav Sablok ◽  
Alvaro Standardi ◽  
Ishfaq Ahmad Hafiz
Keyword(s):  
Plant Growth ◽  
Growth And Development ◽  
Carbon Sources ◽  
Plant Growth And Development ◽  
In Vitro Plant

scholarly journals Topolins and Red Light Improve the Micropropagation Efficiency of Passion Fruit (Passiflora edulis Sims) ‘Tainung No. 1’

HortScience ◽  
2020 ◽  
Vol 55 (8) ◽  
pp. 1337-1344
Author(s):  
Ying-Chun Chen ◽  
Chen Chang ◽  
Huey-Ling Lin
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
In Vitro Culture ◽  
Red Light ◽  
Passion Fruit ◽  
Nodal Segments ◽  
Production Plant ◽  
High Quality ◽  
Red Led

Passion fruit is a commercial crop of economic importance worldwide, with recent increases in demand for high-quality plants for commercial production. Plant tissue culture is widely used for the mass propagation of many commercial crops, however its application on passion fruit is challenged by the problem of low reproducibility, leaf chlorosis, and growth retardation resulted from in vitro culture. The aim of this study was to evaluate the effects of cytokinins and light quality on in vitro culture of nodal segments of passion fruit ‘Tainung No. 1’. Three aromatic cytokinins were tested in a modified MS basal medium. The bud proliferation rates of segments initiated on a media containing 1 mg·L−1 meta-topolin riboside (mTR) or benzyladenine (BA) were not significantly different at the same concentration. Buds cultured on medium supplemented with mTR grew and elongated for 4 weeks, while buds on a medium containing BA formed rosettes. After transfer to a medium without plant growth regulators (PGRs), shoots rooted spontaneously within 8 weeks. Furthermore, the effects of continuous propagation under a high proportion of red light affected the subsequent plant growth. Red LED induced an increase in the chlorophyll content (2.71 mg·g−1) compared with other light qualities (1.05–2.63 mg·g−1) and improved plantlet quality. Acclimated plants were grown in the field, and the flower morphology and fruit set were of commercial quality. Findings showed that replacing BA with mTR as the main cytokinin and using a high proportion of red light during the tissue culture induction period produced high-quality plantlets in 3 months. This system is economical and will be further developed for the commercial propagation of passion fruit vines in the future.

scholarly journals Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Durum Wheat ◽  
Growth And Development ◽  
Indole Acetic Acid ◽  
Greenhouse Experiment ◽  
Hydrocyanic Acid ◽  
In Vitro Assays

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

scholarly journals Application of Light-Emitting Diodes for Improving the Nutritional Quality and Bioactive Compound Levels of Some Crops and Medicinal Plants

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1477
Author(s):  
Woo-Suk Jung ◽  
Ill-Min Chung ◽  
Myeong Ha Hwang ◽  
Seung-Hyun Kim ◽  
Chang Yeon Yu ◽  
...  
Keyword(s):  
Plant Growth ◽  
In Vitro Culture ◽  
Light Emitting Diodes ◽  
Photosynthetic Pigment ◽  
Antioxidant Properties ◽  
Light Sources ◽  
Light Emitting ◽  
Cell Defense ◽  
Led Irradiation

Light is a key factor that affects phytochemical synthesis and accumulation in plants. Due to limitations of the environment or cultivated land, there is an urgent need to develop indoor cultivation systems to obtain higher yields with increased phytochemical concentrations using convenient light sources. Light-emitting diodes (LEDs) have several advantages, including consumption of lesser power, longer half-life, higher efficacy, and wider variation in the spectral wavelength than traditional light sources; therefore, these devices are preferred for in vitro culture and indoor plant growth. Moreover, LED irradiation of seedlings enhances plant biomass, nutrient and secondary metabolite levels, and antioxidant properties. Specifically, red and blue LED irradiation exerts strong effects on photosynthesis, stomatal functioning, phototropism, photomorphogenesis, and photosynthetic pigment levels. Additionally, ex vitro plantlet development and acclimatization can be enhanced by regulating the spectral properties of LEDs. Applying an appropriate LED spectral wavelength significantly increases antioxidant enzyme activity in plants, thereby enhancing the cell defense system and providing protection from oxidative damage. Since different plant species respond differently to lighting in the cultivation environment, it is necessary to evaluate specific wavebands before large-scale LED application for controlled in vitro plant growth. This review focuses on the most recent advances and applications of LEDs for in vitro culture organogenesis. The mechanisms underlying the production of different phytochemicals, including phenolics, flavonoids, carotenoids, anthocyanins, and antioxidant enzymes, have also been discussed.

scholarly journals Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra

2017 ◽  
Vol 69 (1) ◽  
pp. 93-101
Author(s):  
Zexiong Chen ◽  
Juan Lou
Keyword(s):  
Plant Growth ◽  
Blue Light ◽  
White Light ◽  
Growth And Development ◽  
Light Quality ◽  
Red Light ◽  
Floral Transition ◽  
Stem Length ◽  
Pcr Analysis ◽  
Phosphate Synthase

Light is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum lycopersicum (cv. micro-Tom) seedlings were exposed to different light-quality environments, including white light and red light supplemented with blue light (at ratios of 3:1 and 8;1, respectively). Tomatoes grown under red light supplemented with blue light displayed significantly shorter stem length, a higher number of flower buds and rate of fruit set, but an extremely late flowering compared to white-light-grown plants. To illustrate the mechanism underlying the inhibition of stem growth and floral transition mediated by red/blue light, 10 trehalose-6-phosphate synthase (TPS) genes were identified in tomato, and bioinformatics analysis was performed. qRT-PCR analysis showed that SlTPSs were expressed widely throughout plant development and SlTPS1 was expressed at extremely high levels in stems and buds. Further analysis of several flowering-associated genes and microRNAs showed that the expressions of SlTPS1, SlFT and miR172 were significantly downregulated in tomato grown under red and blue light compared with those grown under white light, whereas miR156 transcript levels were increased. A regulatory model underlying vegetative growth and floral transition regulated by light qualities is presented. Our data provide evidence that light quality strongly affects plant growth and phase transition, most likely via the TPS1-T6P signaling pathway.

scholarly journals Critical Photoperiod and Optimal Quality of Night Interruption Light for Runner Induction in June-Bearing Strawberries

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1996
Author(s):  
Yali Li ◽  
Jie Xiao ◽  
Jiangtao Hu ◽  
Byoung Ryong Jeong
Keyword(s):  
Red Light ◽  
Photon Flux ◽  
Photon Flux Density ◽  
Light Emitting ◽  
Red Led ◽  
Critical Photoperiod ◽  
Closed Walk ◽  
White Light Emitting Diodes ◽  
Optimal Quality

The optimal photoperiod and light quality for runner induction in strawberries ‘Sulhyang’ and ‘Maehyang’ were investigated. Two experiments were carried out in a semi-closed walk-in growth chamber with 25/15 °C day/night temperatures and a light intensity of 250 μmol·m–2·s–1photosynthetic photon flux density (PPFD) provided from white light-emitting diodes (LEDs). In the first experiment, plants were treated with a photoperiod of either 12, 14, 16, 18, 20, or 22 h In the second experiment, a total of 4 h of night interruption (NI) light at an intensity of 70 μmol·m–2·s–1PPFD provided from either red, blue, green, white, or far-red LED in addition to 11 h short day (SD). The results showed that both ‘Sulhyang’ and ‘Maehyang’ produced runners when a photoperiod was longer than 16 h, and the number of runners induced positively correlated with the length of photoperiod. However, the plant growth, contents of chlorophyll, sugar and starch, and Fv/Fo decreased in a 22 h photoperiod. All qualities of the NI light, especially red light, significantly increased the number of runners and daughter plants induced per plant as compared with those in the SD treatment in both cultivars. In a conclusion, a photoperiod between 16 and 20 h and NI light, especially red NI light, can be used for quality runner induction in both ‘Sulhyang’ and ‘Maehyang’.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

1976 ◽  
Vol 54 (21) ◽  
pp. 2409-2414 ◽  
Author(s):  
R. M. Behki ◽  
S. M. Lesley
Keyword(s):  
Plant Regeneration ◽  
Lycopersicon Esculentum ◽  
Growth Regulators ◽  
In Vitro Plant Regeneration ◽  
Leaf Discs ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Napthaleneacetic Acid ◽  
In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

Quality and Intensity of Light in the In Vitro Development of Microstumps of Eucalyptus urophylla in a Photoautotrophic System

2020 ◽  
Vol 66 (6) ◽  
pp. 754-760 ◽  
Author(s):  
Natane A Miranda ◽  
Aloisio Xavier ◽  
Wagner C Otoni ◽  
Ricardo Gallo ◽  
Kellen C Gatti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Light Quality ◽  
Stomatal Density ◽  
Carotenoid Content ◽  
Fluorescent Light ◽  
Eucalyptus Urophylla ◽  
Light Emitting ◽  
Fluorescent Lamps ◽  
Number Of Shoots

Abstract The quality and quantity of light are important factors in controlling in vitro plant growth in photoautotrophic systems. The aim of this study was to evaluate the influence of light quality (fluorescent, white, red, blue, red/blue, and distant red) on microstumps of a Eucalyptus urophylla clone in an in vitro photoautotrophic system, as well as the intensity of fluorescent light (60, 85, 100, and 140 μmol m–2 s–1) in the growth and production of microcutting. The number of shoots and microcutting, the size of the largest shoot, the stomatal density, chlorophyll, and carotenoid content were analyzed. Light quality altered plant growth, and fluorescent light intensity did not affect the microstumps’ production during the evaluation period. In white light-emitting diode (LED) light, there was higher production of carotenoids, with a lower initial production of microcuttings. A smaller number of shoots were obtained in blue LED. In general, the different qualities and light intensities tested allowed for the growth of the Eucalyptus urophylla clone grown in vitro, making it possible to obtain microcuttings under photoautotrophic cultivation. Study Implications In vitro propagation is a stressful process for plants and has limitations for commercial-scale Eucalyptus production. Fluorescent lamps, closed containers, and high sucrose concentrations are traditionally used. To reduce costs and improve production, the use of efficient light sources and photoautotrophic cultivation systems become alternatives. This study investigated the influence of light on the in vitro growth of a Eucalyptus clone in a photoautotrophic system. The quality was more important than the intensity of light. Foresters will be able to indicate the use of LEDs (light-emitting diodes) as a replacement for fluorescent lamps. This approach is useful in enhancing micropropagation techniques.

Sign in / Sign up

Export Citation Format

Share Document