Kinovea tracking guide and distance calculation R script v1

Author(s):  
Simon Benmaamar

This protocol can be used to determine the travelled distances of larvae on a black-stained agar plate. For this purpose, videos of the moving animals are recorded, the movement is then tracked using the Kinovea program and the resulting files are evaluated using an R script.

2019 ◽  
Author(s):  
Jordan B Gregg

AIEC-LF82 is a strain of bacteria that is surmised to have a role in causing IBD and Crohn’s disease by activating pro-inflammatory gene expression in organisms. Using antibiotics via combination therapy has been a technique used in clinical settings in an attempt to treat the strains, however, the attempts have not been that effective nor efficient in terms of completely halting the growth and colonization of AIEC to treat IBD and Crohn's disease patients. Research has shown that regarding hindering or preventing the colonization bacterial colonies, sequential therapy tends to be more effective and time-efficient than combination therapy, with fewer adverse effects. To test if this is also the case with the AIEC-LF82 strain of bacteria, I first tested AIEC’s response to combination therapy using the Penicillin-Streptomycin, Kanamycin-Chloramphenicol, antimicrobial peptide (AMP), Kanamycin, SPE phase and LB agar plates, all of which were experimental plates other than the LB agar plate that acted as the negative control. I then tested AIEC-LF82’s response to sequential therapy using the LB+ Kan + Spe, LB + AMP + Spe, LB+ Kan/Cam + Spe, LB + P/S + Spe, LB + P/S + Kan and LB + P/S + AMP and one LB agar plate acting as the negative control. The only differences between sets a and b were the order in which antibiotics were administered in the six aforementioned treatment sets. Ultimately, I found that set b of sequential therapy, strong-weak antibiotic treatments, was the most effective treatment but that set a regarding sequential therapy was actually the least effective of all of the treatments. In conclusion, using strong-weak sequential antibiotic therapy treatments appears to be a potentially promising option to treat patients suffering from Crohn's disease and IBD.


2018 ◽  
Vol 30 (10) ◽  
pp. 1794
Author(s):  
Dejun Zhang ◽  
Fazhi He ◽  
Long Tian ◽  
Zhuyang Xie ◽  
Lu Zou

2020 ◽  
Vol 58 (10) ◽  
pp. 1759-1767
Author(s):  
Mieke Steenbeke ◽  
Sander De Bruyne ◽  
Jerina Boelens ◽  
Matthijs Oyaert ◽  
Griet Glorieux ◽  
...  

AbstractObjectivesIn this study, the possibilities of Fourier-transformed infrared spectroscopy (FTIR) for analysis of urine sediments and for detection of bacteria causing urinary tract infections (UTIs) were investigated.MethodsDried urine specimens of control subjects and patients presenting with various nephrological and urological conditions were analysed using mid-infrared spectroscopy (4,000–400 cm−1). Urine samples from patients with a UTI were inoculated on a blood agar plate. After drying of the pure bacterial colonies, FTIR was applied and compared with the results obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chemometric data analysis was used to classify the different species.ResultsDue to the typical molecular assignments of lipids, proteins, nucleic acids and carbohydrates, FTIR was able to identify bacteria and showed promising results in the detection of proteins, lipids, white and red blood cells, as well as in the identification of crystals. Principal component analysis (PCA) allowed to differentiate between Gram-negative and Gram-positive species and soft independent modelling of class analogy (SIMCA) revealed promising classification ratios between the different pathogens.ConclusionsFTIR can be considered as a supplementary method for urine sediment examination and for detection of pathogenic bacteria in UTI.


Parasitology ◽  
2019 ◽  
Vol 146 (12) ◽  
pp. 1602-1614 ◽  
Author(s):  
Hesham M. Al-Mekhlafi ◽  
Nabil A. Nasr ◽  
Yvonne A. L. Lim ◽  
Fatin Nur Elyana ◽  
Hany Sady ◽  
...  

AbstractThis cross-sectional study aimed to determine the prevalence and risk factors of S. stercoralis infection among 1142 Orang Asli primary schoolchildren in six different states of Peninsular Malaysia. Fecal samples were examined using direct smear, formalin-ether sedimentation (FES), agar plate culture (APC) and PCR techniques. Overall, 15.8% of the children were found to be infected with S. stercoralis. The prevalence was 0.2, 1.3, 15.2 and 13.7% by direct smear, FES, APC and PCR, respectively. Multivariate analysis showed that an age of >10 years, being male, belonging to a Proto-Malay tribe, belonging to the Senoi tribe, indiscriminate defecation, using an unimproved water source for drinking water and not wearing shoes when outside were the significant risk factors of infection among these children. In conclusion, we provide new evidence on the occurrence of S. stercoralis in Malaysia to show that there is a relatively high prevalence of infection among Orang Asli schoolchildren. Therefore, the use of specific methods for detecting S. stercoralis should be considered when screening these children for intestinal parasites. Moreover, prevention and control measures specific to S. stercoralis should be integrated into the intestinal parasitic infections control programme in Malaysia.


2011 ◽  
Vol 11 ◽  
pp. 666-674
Author(s):  
Zhang Jia-qing ◽  
Lu Shou-xiang ◽  
Yuan Man ◽  
Li Chang-hai ◽  
Li Qiang

1950 ◽  
Vol 40 (1-2) ◽  
pp. 150-156 ◽  
Author(s):  
F. L. M. Dawson

The genitalia of a control series of nineteen animals, slaughtered for other reasons than reproductive failure, were studied. Of these six were in various stages of pregnancy, one was in a ‘proliferative phase’, being slaughtered probably just before the first oestrus after calving, and twelve represented different phases of the oestrous cycle, more than half exemplifying the last 4 days before heat. Stages were judged from the appearance of the ovaries, and checked in five instances by repeated rectal examinations, and observation of behaviour during life. Of the nineteen uteri eight yielded bacteria on culture, sometimes in moderately high density; from two of them, pure cultures were recovered respectively of Pseudomonas and Neisseria catarrhalis; and in another, probably Proteus was found. No previous records of these three genera at such a site have been found. Only aerobic blood agar plate cultures, and those for tuberculosis organisms were made. Dissection results unequivocally supported the view of Tagliavini in opposition to that taken by Hammond, that the sanguineous elements in post-oestral discharge originated from endometrial extravasation. The cow slaughtered 4 days after heat indicated that congestion disappears from the caruncles before leaving the areas between them. Microscopically, no mast cells, as observed by the Italian workers, could be seen; it appeared that a ‘proliferative phase’ occurs in every cycle during the three pre-oestral days, when gland tissue proliferates from its nadir of development, surface epithelium grows in height, and vascularization progresses. The rate and interrelations of these changes seemed variable. Arterioles appeared to be withdrawn from the superficial mucosa during the secretory phase. Tagliavini's claim to have observed sloughing of epithelium about the 17th day, strictly equivalent to the process of menstruation in the Primates, must on the evidence be regarded with considerable reserve.


2014 ◽  
Vol 587-589 ◽  
pp. 2156-2159 ◽  
Author(s):  
Tian Xiao ◽  
Ji Shu Sun ◽  
Can Zhang Jin

Glare is one of the most important factors threating expressway traffic safety an night. The most commonly way to prevent glaring night is to set anti-glare plate. Different from the straight sections of expressway, the relationship between the front light of vehicles and the distance of anti-glare plate on the horizontal curved section has some-what changed. Through a lot of tests and finite element simulation, the relationship between the distance of anti-glare plate, horizontal curve radius and anti-glare effect were analyzed systematically. Distance calculation formula of anti-glare plate in horizontal curve sections was revised in this paper. The anti-glare plate distance requirement under different expressway alignment design indexes and its calculation formula was proposed. The achievement was beneficial to confirm the anti-glare effect and improve traffic safety. It can provide us with a reference and a supplement of the specification.


2000 ◽  
Vol 12 (2) ◽  
pp. 118-125 ◽  
Author(s):  
Randall S. Singer ◽  
Wesley O. Johnson ◽  
Joan S. Jeffrey ◽  
Richard P. Chin ◽  
Tim E. Carpenter ◽  
...  

A general problem for microbiologists is determining the number of phenotypically similar colonies growing on an agar plate that must be analyzed in order to be confident of identifying all of the different strains present in the sample. If a specified number of colonies is picked from a plate on which the number of unique strains of bacteria is unknown, assigning a probability of correctly identifying all of the strains present on the plate is not a simple task. With Escherichia coli of avian cellulitis origin as a case study, a statistical model was designed that would delineate sample sizes for efficient and consistent identification of all the strains of phenotypically similar bacteria in a clinical sample. This model enables the microbiologist to calculate the probability that all of the strains contained within the sample are correctly identified and to generate probability-based sample sizes for colony identification. The probability of cellulitis lesions containing a single strain of E. coli was 95.4%. If one E. coli strain is observed out of three colonies randomly selected from a future agar plate, the probability is 98.8% that only one strain is on the plate. These results are specific for this cellulitis E. coli scenario. For systems in which the number of bacterial strains per sample is variable, this model provides a quantitative means by which sample sizes can be determined.


Sign in / Sign up

Export Citation Format

Share Document