VARIATIONS IN TERPENE PROFILES OF DIFFERENT STRAINS OF CANNABIS SATIVA L.

2011 ◽  
pp. 115-121 ◽  
Author(s):  
S. Casano ◽  
G. Grassi ◽  
V. Martini ◽  
M. Michelozzi
Keyword(s):  
Cannabis Sativa ◽  
Different Strains

scholarly journals Selective cannabis strain allergy in a patient presenting with a local allergic reaction

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Peter Stepaniuk ◽  
Amin Kanani
Keyword(s):  
Direct Contact ◽  
Cannabis Sativa ◽  
Skin Prick Testing ◽  
Case Presentation ◽  
Published Report ◽  
Cannabis Indica ◽  
Different Strains ◽  
Allergen Identification ◽  
Strain Dependent ◽  
Fresh Skin

Abstract Background Cannabis use is growing domestically due to recent legalization in many jurisdictions. There are two main species of cannabis, Cannabis sativa and Cannabis indica, and thousands of different commercially available cannabis strains. Although there are multiple reports of cannabis allergy in the literature, to our knowledge, there is no prior published report of selective cannabis strain allergy. Case presentation A 31-year-old male was referred for allergy assessment due to several episodes of localized pruritus and erythema after direct contact with various strains of cannabis. He had noted that the severity of his reaction appeared to be strain dependent. He developed a severe local reaction involving bilateral periorbital edema shortly after coming into direct contact with one particular strain of cannabis. He denied any adverse symptoms after inhalation of cannabis. Fresh skin prick testing was performed to various strains of cannabis and had positive testing to the three of the five tested strains. Conclusions We believe this is the first reported case of selective cannabis strain allergy based on patient history and skin prick testing. This case report outlines the variability in different strains of cannabis and stresses the importance of further research into cannabis allergen identification. Multiple cannabis allergens should be included and incorporated into commercial extracts when they become routinely available.

scholarly journals Impact of Three Different Light Spectra on the Yield, Morphology and Growth Trajectory of Three Different Cannabis sativa L. Strains

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1866
Author(s):  
Philipp Reichel ◽  
Sebastian Munz ◽  
Jens Hartung ◽  
Achim Präger ◽  
Stiina Kotiranta ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Plant Morphology ◽  
Cultivated Plants ◽  
Shade Avoidance ◽  
Light Spectra ◽  
Flower Yield ◽  
R:Fr Ratio ◽  
Lateral Branches ◽  
Different Strains ◽  
Changing Light

Cannabis is one of the oldest cultivated plants, but plant breeding and cultivation are restricted by country specific regulations. Plant growth, morphology and metabolism can be manipulated by changing light quality and intensity. Three morphologically different strains were grown under three different light spectra with three real light repetitions. Light dispersion was included into the statistical evaluation. The light spectra considered had an influence on the morphology of the plant, especially the height. Here, the shade avoidance induced by the lower R:FR ratio under the ceramic metal halide lamp (CHD) was of particular interest. The sugar leaves seemed to be of elementary importance in the last growth phase for yield composition. Furthermore, the last four weeks of flowering were crucial to influence the yield composition of Cannabis sativa L. through light spectra. The dry flower yield was significantly higher under both LED treatments compared to the conventional CHD light source. Our results indicate that the plant morphology can be artificially manipulated by the choice of light treatment to create shorter plants with more lateral branches which seem to be beneficial for yield development. Furthermore, the choice of cultivar has to be taken into account when interpreting results of light studies, as Cannabis sativa L. subspecies and thus bred strains highly differ in their phenotypic characteristics.

scholarly journals Quantitative Determination of Δ9-THC, CBG, CBD, Their Acid Precursors and Five Other Neutral Cannabinoids by UHPLC-UV-MS

Planta Medica ◽  
2017 ◽  
Vol 84 (04) ◽  
pp. 260-266 ◽  
Author(s):  
Yan-Hong Wang ◽  
Bharathi Avula ◽  
Mahmoud ElSohly ◽  
Mohamed Radwan ◽  
Mei Wang ◽  
...  
Keyword(s):  
Formic Acid ◽  
Quantitative Determination ◽  
Cannabis Sativa ◽  
Solvent System ◽  
Plant Sample ◽  
Relative Standard ◽  
Different Strains ◽  
Processing And Storage ◽  
Liquid Chromatographic

AbstractCannabinoids are a group of terpenophenolic compounds in the medicinal plant Cannabis sativa (Cannabaceae family). Cannabigerolic acid, Δ9-tetrahydrocannabinolic acid A, cannabidiolic acid, Δ9-tetrahydrocannabinol, cannabigerol, cannabidiol, cannabichromene, and tetrahydrocannabivarin are major metabolites in the classification of different strains of C. sativa. Degradation or artifact cannabinoids cannabinol, cannabicyclol, and Δ8-tetrahydrocannabinol are formed under the influence of heat and light during processing and storage of the plant sample. An ultrahigh-performance liquid chromatographic method coupled with photodiode array and single quadruple mass spectrometry detectors was developed and validated for quantitative determination of 11 cannabinoids in different C. sativa samples. Compounds 1 – 11 were baseline separated with an acetonitrile (with 0.05% formic acid) and water (with 0.05% formic acid) gradient at a flow rate of 0.25 mL/min on a Waters Cortec UPLC C18 column (100 mm × 2.1 mm I. D., 1.6 µm). The limits of detection and limits of quantitation of the 11 cannabinoids were below 0.2 and 0.5 µg/mL, respectively. The relative standard deviation for the precision test was below 2.4%. A mixture of acetonitrile and methanol (80 : 20, v/v) was proven to be the best solvent system for the sample preparation. The recovery of all analytes was in the range of 97 – 105%. A total of 32 Cannabis samples including hashish, leaves, and flower buds were analyzed.

scholarly journals Strain-, Sex-, and Time-Dependent Antidepressant-like Effects of Cannabidiol

2021 ◽  
Vol 14 (12) ◽  
pp. 1269
Author(s):  
Gabriela P. Silote ◽  
Michelle C. Gatto ◽  
Amanda Eskelund ◽  
Francisco S. Guimarães ◽  
Gregers Wegener ◽  
...  
Keyword(s):  
Selective Breeding ◽  
Cannabis Sativa ◽  
Tail Suspension Test ◽  
Rodent Models ◽  
Tail Suspension ◽  
Resistant Lines ◽  
Antidepressant Effects ◽  
Immobility Time ◽  
Administration Time ◽  
Different Strains

Cannabidiol (CBD) is a non-intoxicating compound extracted from Cannabis sativa, showing antidepressant-like effects in different rodent models. However, inconsistent results have been described depending on the species and the strain used to assess depressive-like behavior. Moreover, only a few studies investigated the effect of CBD in female rodents. Therefore, we aimed to (i) investigate the effects of CBD in two different strains of mice (Swiss and C57BL/6) and a rat model of depression based on selective breeding (Flinders Sensitive and Resistant Lines, FSL and FRL) subjected to tests predictive of antidepressant-like effects and (ii) investigate the influence of sex in the effects of CBD in both mice and rats. CBD induced an antidepressant-like effect in male Swiss but not in female Swiss or C57BL/6 mice in the tail suspension test (TST). In male FSL rats, CBD produced an antidepressant-like effect 1 h post injection. However, in female FSL, CBD induced a bimodal effect, increasing the immobility time at 1 h and decreasing it at 2 h. In conclusion, strain, sex, and administration time affect CBD’s behavioral response to rodents exposed to tests predictive of antidepressant effects.

scholarly journals First Insights Into the Virus and Viroid Communities in Hemp (Cannabis sativa)

2021 ◽  
Vol 3 ◽  
Author(s):  
Judith Chiginsky ◽  
Kaitlyn Langemeier ◽  
Jacob MacWilliams ◽  
Tessa Albrecht ◽  
Whitney Cranshaw ◽  
...  
Keyword(s):  
Crop Production ◽  
Cannabis Sativa ◽  
Management Strategies ◽  
Distinct Group ◽  
Tobacco Streak Virus ◽  
High Nucleotide Sequence Identity ◽  
Streak Virus ◽  
New Genotype ◽  
Different Strains ◽  
Next Generation Sequencing Ngs

Hemp (Cannabis sativa L.) production has increased significantly in recent years; however, the crop has been understudied in the U.S. since its production declined in the late 1950s. Disease identification and management is an increasing challenge for hemp growers across the country. In 2019, beet curly top virus (BCTV) was first reported in hemp in Colorado. Hence, we were motivated to understand the diversity and prevalence of BCTV strains infecting hemp in Colorado. We detected BCTV at high incidence rate (81%) in leaf samples from 12 counties. Two different strains of BCTV, Worland (Wor) and Colorado (CO) were present as a single or mixed infection in hemp leaf samples. Phylogenetic analysis revealed BCTV sequences from hemp formed a distinct group along with BCTV strains CO and Wor. To determine other potential viral and viroid pathogens in hemp, we performed next generation sequencing (NGS). Virome analysis revealed the presence of both virus and viroid sequences that had high nucleotide sequence identity with GenBank accessions for cannabis cryptic virus, cannabis sativa mitovirus, citrus yellow vein associated virus, opuntia-like virus and hop latent viroid. In contrast, tobacco streak virus sequences were highly variable compared to sequences in GenBank suggesting a possible new genotype of this virus. The data presented here has important implications for the epidemiology and management of the various diseases of hemp and will lead to the development of integrated pest management strategies designed to interrupt transmission cycles and facilitate efficient crop production.

scholarly journals Strain, Sex, and Time Dependent Antidepressant-like Effects of Cannabidiol

2021 ◽  
Author(s):  
Gabriela Pandini Silote ◽  
Michelle C. Gatto ◽  
Amanda Eskelund ◽  
Francisco S. Guimarães ◽  
Gregers Wegener ◽  
...  
Keyword(s):  
Selective Breeding ◽  
Cannabis Sativa ◽  
Tail Suspension Test ◽  
Rodent Models ◽  
Resistant Lines ◽  
Antidepressant Effects ◽  
Immobility Time ◽  
Administration Time ◽  
Different Strains ◽  
Different Doses

Cannabidiol (CBD) is a non-intoxicating compound extracted from Cannabis sativa, showing antidepressant-like effects in different rodent models. However, inconsistent results have been described depending on the species and the strain used to assess depressive-like behaviour. Moreover, only a few studies have investigated the effect of CBD in female rodents. Therefore, we aimed to i) investigate the effects of CBD in two different strains of mice (Swiss and C57BL/6) and in a rat model of depression based on selective breeding (Flinders Sensitive and Resistant Lines, FSL and FRL) subjected to tests predictive of antidepressant-like effects; and ii) investigate the influence of sex in the effects of CBD in both mice and rats. CBD induced an antidepressant-like effect in male Swiss but not in female Swiss or C57BL/6 mice in the tail suspension test (TST). In male FSL rats, CBD produced an antidepressant-like effect one-hour post-injection. However, in female FSL, CBD induced a bimodal effect, increasing the immobility time at one hour and decreasing it at two hours. Ketamine produced an antidepressant-like effect in male and female FSL rats at different doses. In conclusion, strain, sex, and administration time affect CBD's behavioural response to rodents exposed to tests predictive of antidepressant effects.

scholarly journals Cannabidiol: From Drug Interaction Potential to Modulation of the Gut Microbiome

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 418-418
Author(s):  
Igor Kortubash ◽  
Charles Skinner ◽  
Laura Ewing ◽  
Mitchell McGill ◽  
Stefanie Kennon-McGill ◽  
...  
Keyword(s):  
Drug Interaction ◽  
Relative Abundance ◽  
Interaction Potential ◽  
Gut Microbiome ◽  
Cannabis Sativa ◽  
Inflammatory Responses ◽  
Bacterial Species ◽  
Proximal Jejunum ◽  
Significant Drug Interaction ◽  
Different Strains

Abstract Objectives Cannabidiol (CBD) is the major non-psychotropic phytocannabinoid present in Cannabis sativa. In 2018, Congress designated select C. sativa cultivars as “hemp” removing them from the DEA's list of controlled substances. As a result, CBD-containing hemp extracts and other CBD products are now widely available and heavily marketed, yet their FDA regulatory status is still hotly debated. Further complicating the debate is CBD's vastly under-researched safety profile. Safety concerns yet to be adequately addressed include CBD's drug interaction potential and its effect on the gut microbiome. Methods Using acetaminophen (APAP), the most commonly ingested over-the-counter pain medication, we demonstrated that CBD-rich cannabis extract (CRCE) poses a significant drug interaction risk. Results Mice exposed to both CRCE and APAP developed severe liver injury. This hepatotoxicity, however, was not observed when either CRCE or APAP were administered separately. Importantly, this injury was observed in two different strains of mice with susceptibilities seemingly linked to sex (female) and age (older animals). Furthermore, both beneficial and adverse effects of CRCE on the gut microbiome were observed. Specifically, CRCE exposure increased the relative abundance of the beneficial gut microbe, Akkermansia muciniphila, however, an overall decrease in the relative abundance of all gut bacterial species was noted. This decrease was paralleled by numerous pro-inflammatory responses in the proximal jejunum and colon. Conclusions Taken together, these findings raise significant concerns about the safety of widespread CBD usage and underlines the need for additional well-designed studies into its safety and efficacy. Funding Sources NIGMS 1P20 GM109005.

Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID

Planta Medica ◽  
2017 ◽  
Vol 84 (04) ◽  
pp. 250-259 ◽  
Author(s):  
Elsayed Ibrahim ◽  
Waseem Gul ◽  
Shahbaz Gul ◽  
Brandon Stamper ◽  
Ghada Hadad ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Limit Of Detection ◽  
Herbaceous Plant ◽  
University Of Mississippi ◽  
Detection Range ◽  
Cannabidiolic Acid ◽  
Limit Of Quantitation ◽  
Different Strains ◽  
The University ◽  
Relationship Of

AbstractCannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of C. sativa, with different concentration ratios of CBD and Δ9-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C. sativa extracts. The method involves trimethyl silyl derivatization of the extracts. These cannabinoids include tetrahydrocannabivarian, CBD, cannabichromene, trans-Δ8-tetrahydrocannabinol, Δ9-THC, cannabigerol, cannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area ratio with R2 > 0.999 for all 10 cannabinoids. The precision and accuracy of the method were found to be ≤ 15% and ± 5%, respectively. The limit of detection range was 0.11 – 0.19 µg/mL, and the limit of quantitation was 0.34 – 0.56 µg/mL for all 10 cannabinoids. The developed method is simple, sensitive, reproducible, and suitable for the detection and quantitation of acidic and neutral cannabinoids in different extracts of cannabis varieties. The method was applied to the analysis of these cannabinoids in different parts of the micropropagated cannabis plants (buds, leaves, roots, and stems).

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

Cannabis und Schizophrenie

2002 ◽  
Vol 48 (5) ◽  
pp. 336-345 ◽  
Author(s):  
Jürgen Seifert ◽  
Jann Schlimme ◽  
Felix Wedegärtner ◽  
Hinderk M. Emrich ◽  
Udo Schneider
Keyword(s):  
Cannabis Sativa ◽  
Cannabinoid System ◽  
Psychotische Störungen

Überblick: Cannabis sativa ist eine der ältesten medizinischen Heilpflanzen. In den westlichen Kulturen wird Cannabis überwiegend als Freizeitdroge angesehen. Im Harz sowie in den Blüten der weiblichen Pflanze findet sich ein Gemisch aus verschiedenen psychoaktiven Inhaltsstoffen. Am bedeutendsten ist in diesem Zusammenhang das delta-9-Tetrahy-drocannabinol. </P><P> Epidemiologie: Nicht selten werden die Gefahren des Cannabiskonsums, wie z. B. die Möglichkeit der Induktion einer Psychose unterschätzt. Wenn man jedoch berücksichtigt, wie häufig z. B. Jugendliche THC konsumieren, ist die Frage, ob Cannabis eine Psychose aus dem schizophrenen Formenkreis de novo induzieren kann, von besonderem gesundheitspolitischem und volkswirtschaftlichem Interesse. Die Datenlage ist allerdings nach wie vor unklar. Die klinisch-epidemiologischen Studien deuten aber darauf hin, dass Cannabiskonsum bei vulnerablen Personen schizophrenieforme Psychosen auslösen kann und einen ungünstigen Krankheitsverlauf zur Folge hat. </P><P> Das endogene Cannabinoid-System: Die vorliegende Arbeit gibt außerdem einen Überblick der Publikationen, die sich mit dem körpereigenen Cannabinoid- System und seinen endogenen Liganden im Hinblick auf psychotische Störungen beschäftigen.

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