Enameloplasty effects on microleakage of pit and fissure sealants under load: an in vitro study

2000 ◽  
Vol 24 (4) ◽  
pp. 279-285 ◽  
Author(s):  
Christina Zervou ◽  
Gerald Kugel ◽  
Cataldo Leone ◽  
Athanasios Zavras ◽  
Eileen Doherty ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Conventional Technique ◽  
Control Group ◽  
Fissure Sealants ◽  
Occlusal Force ◽  
Pit And Fissure Sealants ◽  
Significant Difference ◽  
Group A ◽  
Group D

Enameloplasty has been implicated in the successful application of pit and fissure sealants. The present study aimed to evaluate the effect of enameloplasty technique on microleakage of sealants when occlusal force was applied on the teeth. The study also allowed a direct comparison of the effectiveness of enameloplasty, when two different burs were used. Six groups of ten human extracted wisdom teeth were tested. Group A: no load, conventional (no enameloplasty - control); Group B: no load, enameloplasty 1/2 round bur (Brasseler USA); Group C: no load, enameloplasty diamond fissure bur REF/UP 791( Ultradent); Group D: load 500 N, conventional , Group E: load 500 N, 1/2 round bur; Group F: load 500 N, diamond fissure bur. Specimens were thermocycled for 500 cycles at 5° and 55° with a dwell time of 30 seconds after load application. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underling fissure. The Kruskal-Wallis One Way Anova and Mann -Whitney U test showed group D having the greatest degree of microleakage statistically significant (p<0.05) comparing with other groups. Sealants prepared with the conventional technique (A, D) had statistically significant differences in microleakage with those prepared with enameloplasty (B,C,E, F) (p = 0.01). Enameloplasty in groups with no load (B, C) did reduce microleakage, but not significantly (p = 0.3) However, loaded teeth with enameloplasty appeared to perform superior, as compared to those without (p=0.005) Regarding the effect of load, teeth without load (Groups A,B, C) were found to have significantly less microleakage than teeth where mechanical force had been applied (Groups D, E, F), (p = 0.01). Microleakage in Group A (conventional technique without load) was significantly less than microleakage in Group D (conventional technique with load), (p = 0.04). However, load did not seem to influence microleakage when enameloplasty had been performed. This finding was true for both round bur enameloplasty (p = 0.29), and fissure bur enameloplasty (p = 0.26). There was no statistically significant difference between Groups B, E (round bur) and C, F (fissure bur) (p > 0.05), or between Groups B and C (p > 0.05) and between Groups E and F (p > 0.05). The results of the study indicated that enameloplasty reduced microleakage of pit and fissure sealants, especially when load was applied to teeth, irrespective of what bur was used to enlarge the fissure, as there was no statistical significant differences between the round and fissured diamond burs. The application of occlusal force to the tooth produces significantly more microleakage, unless enameloplasty is performed.

An in vitro study of microleakage of pit and fissure sealants in the presence of occlusal forces

2000 ◽  
Vol 24 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Christina Zervou ◽  
Eileen Doherty ◽  
Athanasios Zavras ◽  
George White
Keyword(s):  
Cross Section ◽  
In Vitro Study ◽  
Fissure Sealants ◽  
Pit And Fissure Sealants ◽  
Dye Penetration ◽  
Wisdom Teeth ◽  
Group A ◽  
Group B ◽  
Group D

The purpose of this study was to evaluate the effect of load on microleakage of pit and fissure sealants. In the first phase of the study load of maximal force (F = 880 N) on central and peripheral enamel was applied. Three groups of ten specimens were tested for microleakage. Group A no application of load, Group B application of load on the central pit of the tooth, and Group application of load on the peripheral enamel 2.5 mm away from the margin of the tooth, at the level of dentinoenamel junction. The results of that study showed significant increase of microleakage on the peripheral sealed enamel, as well as enamel microcracks. So, in the phase II sixty wisdom teeth were sealed with Ultra seal XT plus and load of different magnitude was applied on the peripheral enamel. The specimens were thermocycled for 500 cycles at 5°C and 55°C with a dwell of 30 seconds at each temperature prior to load application. Six groups of ten specimens were tested for microleakage under load. The force was applied at the peripheral enamel, 2.5 mm away from the margin of the tooth, at the level of dentin-enamel junction in cross-section view. Group A: F = 0 N; Group B: F = 300 N; Group C: F = 400 N; Group D: F = 500 N; Group E: F = 600 N; Group F: F = 700 N. Microleakage was scored as distance of dye penetration with 0 = no microleakage and 3 = microleakage to underlying fissure. A linear regression statistics was used with R-squared of 0.31. The groups with load applied (B, C, D, E, F) showed statistically significant results (p<0.05) compared with group A (F = 0 N). Both, the magnitude of the force and tooth morphology may influence the degree of microleakage observed after the placement of sealants.

EVALUATION OF THE EFFECT OF FERROUS SULFATE ON ENAMEL DEMINERALIZATION OF HUMAN DECIDUOUS TEETH: AN IN VITRO STUDY

2017 ◽  
Vol 8 (3) ◽  
pp. 83-89
Author(s):  
Johnny Holanda De Gauw ◽  
Lara Maria Melo Costa ◽  
Rodrigo Neves Silva ◽  
Natanael Barbosa Santos ◽  
Maria Dânia Holanda Tenorio
Keyword(s):  
Ferrous Sulfate ◽  
Polarized Light ◽  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Group A ◽  
Ph Cycling ◽  
Group B ◽  
Group D

Objective: This study aimed to evaluate the effect of ferrous sulfate (FS) on demineralized and non-demineralized human deciduous teeth. Additionally, it was evaluated the penetration extent of FS and its remineralizing effect on the enamel of deciduous teeth using Polarized Light Microscopy (PLM). Method: The sample comprised 44 human deciduous teeth. The 44 crowns were divided randomly into four groups: group A (FS after demineralization), group B (FS without demineralization), group C (only demineralization), and group D (control group). FS at 0.45 mol/L-1 was used daily (15 days) and demineralization was done by pH cycling (7 days). Then, three longitudinal slices of the crowns were photographed using PLM. The degree of penetration of the lesion or stain was measured in micrometers, as well as the distance between the external enamel surface and the core of lesion. Results: Group A showed a dark stain on the outer surface of enamel larger than the group B. It is suggested, a remineralizing effect when comparing groups, A and C. The mean depth and standard deviation for groups A, B, and C were 4.27µm (±1.49), 3.72 µm (±1.68) and 5.00 µm (±1.84), respectively. No dark stains were observed in group D. Conclusion: FS stained the demineralized and non-demineralized human deciduous teeth. However, dark stains in the non-demineralized teeth were smaller or absent, than in the demineralized teeth. Therefore, FS may have a protective effect against demineralization.

The Effect of Varying Ferrule Modes on Fracture Resistance of Canines Restored with One-Piece Milled Zirconia Post and Core

2012 ◽  
Vol 624 ◽  
pp. 98-102
Author(s):  
Yao Kun Zhang ◽  
Long Quan Shao ◽  
Ruo Yu Liu ◽  
Lin Lin Wang ◽  
Jun Ai ◽  
...  
Keyword(s):  
Fracture Resistance ◽  
In Vitro Study ◽  
Significant Difference ◽  
Zirconia Crowns ◽  
Group A ◽  
Post And Core ◽  
Resistance Decrease ◽  
Group B ◽  
Group D

The aim of this invitro study was to evaluate the fracture resistance of endodontically treated canines restored with one-piece milled zirconia post and core in the varying ferrule modes. Fifty recently extracted human maxillary canines were endodontically treated and randomly divided into 5 groups of 10 specimens each. According to the defect degree of ferrule, intact ferrule was classified as group A and served as control, 2 mm ferrule height without buccal ferrule was classified as group B, 2 mm ferrule height without mesial and buccal ferrule was classified as group C, 2 mm ferrule height without mesial, buccal and lingual ferrule was classified as group D, no ferrule preparation was classified as group E. All of the teeth were restored with one-piece milled zirconia post and core, and zirconia crowns. The teeth were prepared to standardized specifications. The restored teeth were loaded to fracture at a 135° angle to their long axis, at a cross-head speed 0.5 mm/min, and the load (N) at failure was recorded. Statistical analysis was performed by SNK pairwise multiple comparisons (α=0.05). The mean fracture resistance of five groups as follows: 1019.30±139.01N for group A, 861.20±105.67N for group B, 833.70±100.56N for group C, 733.20±96.91N for group D, 698.00±99.90N for group E. The fracture resistance of restored teeth between group B and group C, group D and group E had no statistically significant difference(P>0.05). The fracture resistance of the rest pairwise group comparisons had statistically significant difference(P<0.05). Increasing the absence degree of ferrule, it was found that the fracture resistance decrease. The buccal and lingual ferrule were more important than mesial ferrule for enhanced fracture resistance. This in vitro study supports evidence that reserving the buccal and lingual ferrule is a key point to increase the fracture resistance of canines restored with one-piece milled zirconia post and core.

scholarly journals Assessing Fracture Resistance of Restored Premolars with Novel Composite Materials: An In Vitro Study

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Zahraa Abdulaali Al-Ibraheemi ◽  
Huda Abbas Abdullah ◽  
Nada Abdlameer Jawad ◽  
Julfikar Haider
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
In Vitro Study ◽  
Vitro Study ◽  
Resistance Testing ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Group D

During restorative treatment, premolars restored with resin filling materials using the conventional incremental-fill technique take longer restoration time and undermine the integrity of the tooth. The aim of this study was to assess fracture resistance of premolars restored by various types of novel bulk-fill composite resin materials. Forty-eight (n = 48) freshly extracted sound maxillary first premolars were used in this in vitro study. The teeth were divided into six groups, each having 8 specimens. Group A (positive control) was allocated for the intact teeth. For specimens in Groups B to F, a large cavity (Class-II MOD) was prepared with a standardized dimension of cavity (3 mm depth on the pulpal floor, 4 mm at the gingival seat, and 3 mm cavity width). Group B represented prepared teeth without any restoration. Group C, Group D, Group E, and Group F were restored with Tetric EvoCeram® incremental-fill (conventional), Beautifil bulk-fill, Filtek posterior bulk-fill, and SonicFill 2 bulk-fill restorative materials, respectively. All samples were finished and polished with an enhanced finishing kit and stored in distilled water for a month before the fracture resistance testing. All the samples were exposed to the axial loading (the speed of crosshead was 1 mm/min) in a computer-controlled universal testing machine (LARYEE, China) via a steel bar (6 mm in diameter) and the maximum applied force in Newton was recorded as the fracture resistance. One-way analysis of variance (SPSS 21) was used to compare the fracture resistance within the groups, and Tukey’s post hoc test was used to determine the difference between the groups. The lowest value of fracture resistance was recorded for Group B, and the highest value was recorded for Group A followed by the values of Group D, Group C, Group F, and Group E. One-way ANOVA revealed a statistically significant difference between the groups ( P < 0.05 ). Nonsignificant difference was found between the premolars restored by bulk-fill and conventional composites. Among the bulk-fill restored specimens, Beautifil restorative demonstrated significantly higher fracture resistance in comparison with the other two bulk-fill restored specimen groups (SonicFill 2 and Filtek). Bulk-fill composite such as Beautifil could be an alternative option to conventional incremental-fill composite for premolar restoration.

scholarly journals Zeolite as a Bone Bio-Modifier Carrier: An In-Vitro Study

2019 ◽  
Vol 9 (1) ◽  
pp. 126
Author(s):  
Mobina Mousavi ◽  
Azadeh Esmaeil Nejad ◽  
Erfan Shamsoddin ◽  
Mohammad Mehdi Golabgiran ◽  
Behzad Houshmand
Keyword(s):  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Alizarin Red ◽  
Group A ◽  
Group B ◽  
And Function ◽  
Group D

Background: Zeolite is a microporous aluminosilicate compound which has been successfully used in tissue engineering. The effects of Zeolite on the morphology and functions of pre-osteoblastic MG-63 cells as new bone enhancer material is still unclear. Methods: In this vitro experimental study, MTT and Alizarin red staining test were performed on six groups of MG-63 cells which differed in Zeolite (Z) concentration and the presence or absence of Alloplast extract (A). Group A: 0.1&mu;g/mL Z+A, Group B: 0.1&mu;g/mL Z without A, Group C: 0.2&mu;g/mL Z+A, Group D: 0.2&mu;g/mL Z without A, Group E: 0.3&mu;g/mL Z+A, Group F: 0.3&mu;g/mL Z without A. There were also three control groups as positive control, negative control, and Alloplast control based on each related test. The data were analyzed by SPSS 20 via one-way ANOVA and Welch test. (P&lt;0.05). Results: At 24 hours, results showed that solutions with 0.1&mu;g/mL, 0.2&mu;g/mL, and 0.3&mu;g/mL Zeolite with or without Alloplast had significantly higher proliferation rates than positive control (distilled water) groups without Alloplast (p&lt;0.001). At 72hours time point, the results showed significantly higher proliferation rates in the solutions with 0.1&mu;g/mL, 0.2&mu;g/mL, and 0.3&mu;g/mL Zeolite with or without Alloplast compared to the positive control group without Alloplast (p&lt;0.001). Conclusions: Zeolite can increase proliferation of MG-63 cells without presence of Alloplast; It seems that combination of Zeolite with Alloplast maybe enhancing proliferation and function of MG-63 cells.

Sealing Ability of ProRoot MTA and MTA-Angelus Simulating a One-Step Apical Barrier Technique- An in vitro Study

2009 ◽  
Vol 33 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Nikita Lolayekar ◽  
Sham Bhat ◽  
Sundeep Hegde
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Control Group ◽  
Blue Dye ◽  
Root Segments ◽  
Sealing Ability ◽  
Significant Difference ◽  
Group A ◽  
Group B

Endodontic treatment of the pulpless tooth with an immature root apex poses a special challenge for the clinician due to lack of an apical stop against which to compact an interim dressing of calcium hydroxide, or the final obturation material. A one-visit apexification protocol with MTA (Mineral Trioxide Aggregate) has be seen as an alternative to the traditional treatment practices with calcium hydroxide [Ca(OH)2]. The aim of this study was to investigate the use of MTA as a 5mm apical barrier by comparing sealing ability of ProRoot MTA to that of MTA-Angelus. 50 freshly extracted Maxillary and Mandibular single canal sound human incisor teeth were used in this study, which were decoronated to standardized 10mm root lengths. The root segments were prepared to simulate the clinical situation of an open apex with Gates Glidden burs # 5-1 and randomly assigned into 2 experimental groups of 20 samples each (Group A: ProRoot MTA, Group B: MTA-Angelus) and 10 root segments were used as control (Group C) where no apical barrier was used. After obturation the coronal portion of all samples were sealed with Glass Ionomer and stored at 37°C and 100% humidity for 4 weeks. The root segments were then double coated with nail varnish except for the apical 1mm and apical ends of all root segments were suspended vertically in methylene blue dye for 48 hours at room temperature. After removal from the dye, the samples were sectioned longitudinally and linear dye penetration was measured using a Grid and stereomicroscope. The measurements were tabulated and statistically analyzed. The mean percentage of dye leakage for Group A was 55.5% and for Group B was 53.25%. The results showed no statistically significant difference in the sealing ability of ProRoot MTA and MTA-Angelus when used as apical barriers.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

2015 ◽  
Vol 85 (6) ◽  
pp. 920-926 ◽  
Author(s):  
Ricardo Carvalho Bueno ◽  
Roberta Tarkany Basting
Keyword(s):  
Chemical Composition ◽  
In Vitro Study ◽  
Chemical Components ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Mini Implants ◽  
Significant Difference ◽  
Implant Anchorage ◽  
Positive Effect

ABSTRACT Objective:  To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods:  Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n  =  3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results:  Two-way ANOVA showed no significant interaction between the factors studied (P  =  0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P  =  .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P &lt; .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions:  Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

scholarly journals Hydroxyl and calcium ions diffusion from endodontic materials through roots of primary teeth - in vitro study

2005 ◽  
Vol 13 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Ana Cristina Gerent Petry Nunes ◽  
Maria José de Carvalho Rocha
Keyword(s):  
In Vitro Study ◽  
Deciduous Teeth ◽  
Control Group ◽  
Apical Surface ◽  
Working Length ◽  
Group A ◽  
Periapical Healing ◽  
Materials Used ◽  
Spectrometer Data

The purpose of this research was to evaluate the diffusion of calcium (Ca+2) and hydroxyl (OH-) ions from materials with a calcium hydroxide base - Ca(OH)2 through the intact roots of deciduous teeth. This diffusion of ions is important for periapical healing. Forty-six deciduous teeth were selected and instrumented to their working length with #40 files. The teeth were washed during cleaning and shaping with a 1% sodium hypochlorite (NaOCl) solution. The canals were dried with paper points. The teeth were divided into 4 groups based on the sealer type, with 10 specimens in each group. A fifth group of 6 teeth without sealer constituted the control group. The materials used as sealers were: Ca(OH)2 paste associated to propylene glycol (CaPE) thickened at the proportion of 2:1 w/v; UFSC (Federal University of Santa Catarina) paste - a mixture of 0.3g of zinc oxide with 0.3g of Ca(OH)2 with 0.2ml of olive oil 1:1 w/w; Vitapex® and Sealapex®. The coronal access was sealed with a glass ionomer after the root had been filled with each sealer. A one-third apical surface and foramen was hard-pressed with Araldite®. The teeth were stored individually in flasks containing saline solution at 37ºC and 100% humidity. The OH- and Ca+2 ions diffusion levels were determined using a pH meter and an atomic absorption spectrometer. Data were collected at 48 hours and at intervals of 7, 30, 45 and 60 days. Statistical analysis was performed using ANOVA to compare groups. In the pH evaluation, the CaPE group presented the largest OH- ions diffusion, which peaked at sixty days (p=0.0309), when compared to the other groups (p<0.0001). In relation to amount of Ca+2 ions released, the CaPE paste showed the best results, followed by the UFSC's paste. These results suggest that the CaPE paste was the material that allowed the highest diffusion of OH- and Ca+2 ions.

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