scholarly journals Changesin Myoglobin of Big Eye Tuna During Chilling Storage

2015 ◽  
Vol 17 (3) ◽  
pp. 214
Author(s):  
Stevi Imelda Murniati Wodi ◽  
Wini - Trilaksani ◽  
Mala - Nurilmala
Keyword(s):  
Molecular Weight ◽  
Value Added ◽  
Water Soluble ◽  
Small Molecular Weight ◽  
Thunnus Obesus ◽  
Dorsal Area ◽  
Molecular Weight Difference ◽  
Chilling Temperatures ◽  
The Tropics ◽  
Ventral Area

Big eye tuna (Thunnus obesus) is one of the species of tuna which is have some value added such as have potential to improve animal protein sources, have high economic values as well as an export commodity.Mishandling and misapplication of high temparatures on the tuna handling at the tropics and sub tropics climate was significantly decreasing the value of myoglobin and affecting the solubility of protein. Myoglobin is a globular protein that have small molecular weight size and it was an important factor for determining the quality of meat and influencing factors of purchasing power by the consumer. The purpose of this experiments is to determining the changes of myoglobin content and the water soluble proteins content at some parts of big eye tuna in 9 days chilling temperatures. The portion which is analized was the ventral area, dorsal area and tail area. Myoglobin content in all portion above, both light and dark meat was analized. The results shows the decreased value of myoglobin content from first handling (day zero) until day ninth (days 9th) experiment. Each myoglobin contentfrom white meat at at ventral, dorsal and tail meat was decreased from 121.68 mg/100 into 41.35 mg/100, 148.2 mg/100g into 52.01 mg/100g, 105.16 mg/100g into 31.34 gr/100gram, after day ninth. The myoglobin content from dark meat at ventral, dorsal and tail meat, was decreased, too ; from 418.64 mg/100 gr into 121.01 mg/100 g, 446.21 mg/100 g into 58.34 mg/100 r and 145.65 mg/100 gr into 87.98 mg/100g after day ninth.Water soluble protein was derived into protein bands with molecular weight 15,4 kDa and 14 kDa. Its suspected as the myoglobin protein. The molecular weight difference was caused from degradation of protein during the storage.<br />Keywords: Big eye tuna, meat, storage, myoglobin

scholarly journals Preparation and Characterization of Polylactic Acid Microspheres Containing Water-Soluble Anesthetics with Small Molecular Weight.

1997 ◽  
Vol 45 (3) ◽  
pp. 513-517 ◽  
Author(s):  
Takahiro UCHIDA ◽  
Kazushi YOSHIDA ◽  
Yoichi NAKADA ◽  
Noriko NAGAREYA ◽  
Yuriko KONISHI ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Polylactic Acid ◽  
Water Soluble ◽  
Small Molecular Weight

scholarly journals Elucidating mechanisms of chlorine toxicity: reaction kinetics, thermodynamics, and physiological implications

2010 ◽  
Vol 299 (3) ◽  
pp. L289-L300 ◽  
Author(s):  
Giuseppe L. Squadrito ◽  
Edward M. Postlethwait ◽  
Sadis Matalon
Keyword(s):  
Molecular Weight ◽  
Chemical Reactivity ◽  
Water Soluble ◽  
Biological Molecules ◽  
Amino Acid Residues ◽  
Small Molecular Weight ◽  
Chlorine Toxicity ◽  
Biological Substrate ◽  
Potential Benefits ◽  
Swimming Pool Water

Industrial and transport accidents, accidental releases during recreational swimming pool water treatment, household accidents due to mixing bleach with acidic cleaners, and, in recent years, usage of chlorine during war and in acts of terror, all contribute to the general and elevated state of alert with regard to chlorine gas. We here describe chemical and physical properties of Cl2 that are relevant to its chemical reactivity with biological molecules, including water-soluble small-molecular-weight antioxidants, amino acid residues in proteins, and amino-phospholipids such as phosphatidylethanolamine and phosphatidylserine that are present in the lining fluid layers covering the airways and alveolar spaces. We further conduct a Cl2 penetration analysis to assess how far Cl2 can penetrate the surface of the lung before it reacts with water or biological substrate molecules. Our results strongly suggest that Cl2 will predominantly react directly with biological molecules in the lung epithelial lining fluid, such as low-molecular-weight antioxidants, and that the hydrolysis of Cl2 to HOCl (and HCl) can be important only when these biological molecules have been depleted by direct chemical reaction with Cl2. The results from this theoretical analysis are then used for the assessment of the potential benefits of adjuvant antioxidant therapy in the mitigation of lung injury due to inhalation of Cl2 and are compared with recent experimental results.

Production of hemicellulose oligomers from softwood chips using autohydrolysis followed by an enzymatic post-hydrolysis

Holzforschung ◽  
2017 ◽  
Vol 71 (7-8) ◽  
pp. 575-581 ◽  
Author(s):  
Vivien Deloule ◽  
Christine Chirat ◽  
Claire Boisset ◽  
Bertrand Toussaint ◽  
Jadwiga Chroboczek
Keyword(s):  
Molecular Weight ◽  
Chemical Composition ◽  
Enzymatic Hydrolysis ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Value Added ◽  
Degree Of Polymerization ◽  
Water Soluble ◽  
Time Parameters ◽  
Temperature Time

AbstractIn the context of value added valorization of hemicelluloses (HCs), their soft extraction by autohydrolysis (AH) of softwood (SW) chips has been optimized via the temperature/time parameters (170°C/2 h, 170°C/1 h and 150°C/1 h). Two enzyme mixtures containing mainly a glucanase and a mannanase were used to decrease the degree of polymerization (DP) of the extracted HCs. Hydrolysates containing HCs were analyzed in terms of monomers and oligomers, molecular weight distribution (MWD) and chemical composition. The MW was strongly dependent on AH conditions: most of the water-soluble HCs with 1800 Da MW were obtained at 150°C/1 h. The parameters 170°C/2 h gave rise to MWs<1800 Da. Enzymatic hydrolysis (EH) reduced efficiently the DP of HCs, and the glucosidase was more efficient than the mannanase, but the former also hydrolyzed more oligomers into their monomeric components.

ANALISA KANDUNGAN BETA-GLUKAN LARUT AIR DAN LARUT ALKALI DARI TUBUH BUAH JAMUR TIRAM (Pleurotus ostreatus) DAN SHIITAKE (Lentinus edodes)

2013 ◽  
Vol 13 (3) ◽  
Author(s):  
Netty Widyastuti ◽  
Teguh Baruji ◽  
Henky Isnawan ◽  
Priyo Wahyudi ◽  
Donowati Donowati
Keyword(s):  
Molecular Structure ◽  
Molecular Weight ◽  
Immune System ◽  
Pleurotus Ostreatus ◽  
Water Soluble ◽  
Low Molecular Weight ◽  
Lentinus Edodes ◽  
Beta Glucan ◽  
Oyster Mushrooms ◽  
The Difference

Beta glucan is a polysaccharide compound, generally not soluble inwater and resistant to acid. Beta glucan is used as an immunomodulator (enhancing the immune system) in mammals is usually a beta-glucan soluble in water, easily absorbed and has a low molecular weight. Several example of beta-glucan such as cellulose (β-1 ,4-glucan), lentinan (β-1 0.6-glucan) and (β-1 ,3-glucan), pleuran (β-1, 6 and β-1 ,3-glucan) are isolated from species of fungi Basidiomycota include mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes).The purpose of thisresearch activity is to obtain beta-glucan compound that can be dissolved in water and in alkali derived from fungi Basidiomycota, i.e, Oyster mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes). The result of beta-glucan compared to characterize the resulting beta glucan that is molecular structure . The difference of beta glucan extraction is based on the differences in solubility of beta-glucan. Beta glucan could be water soluble and insoluble water.

Hemostatically potent small molecular weight serine protease from Maclura spinosa (Roxb. ex Willd.) accelerates healing of subcutaneous dermal wounds in Swiss albino mice

Biologia ◽  
2019 ◽  
Vol 75 (1) ◽  
pp. 139-149
Author(s):  
Venkatesh Bommalapura Kulkarni ◽  
Raghu Ram Achar ◽  
Maheshwari Mahadevappa ◽  
Dinesh Sosalagere Manjegowda ◽  
Priya Babu Shubha ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Serine Protease ◽  
Small Molecular Weight ◽  
Swiss Albino Mice ◽  
Albino Mice

scholarly journals Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure

2021 ◽  
Vol 14 (4) ◽  
pp. 301
Author(s):  
Yayoi Kawano ◽  
Viorica Patrulea ◽  
Emmanuelle Sublet ◽  
Gerrit Borchard ◽  
Takuya Iyoda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Weight ◽  
Wound Healing ◽  
Hyaluronic Acid ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Water Soluble ◽  
Wound Healing Process ◽  
And Migration ◽  
Proliferation And Migration

Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds.

scholarly journals Investigating Lignin-Derived Monomers and Oligomers in Low-Molecular-Weight Fractions Separated from Depolymerized Black Liquor Retentate by Membrane Filtration

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2887
Author(s):  
Kena Li ◽  
Jens Prothmann ◽  
Margareta Sandahl ◽  
Sara Blomberg ◽  
Charlotta Turner ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Performance ◽  
Membrane Filtration ◽  
Black Liquor ◽  
Complex Mixture ◽  
Value Added ◽  
Kraft Pulping ◽  
Classification Model ◽  
Low Molecular Weight ◽  
Chemical Complexity

Base-catalyzed depolymerization of black liquor retentate (BLR) from the kraft pulping process, followed by ultrafiltration, has been suggested as a means of obtaining low-molecular-weight (LMW) compounds. The chemical complexity of BLR, which consists of a mixture of softwood and hardwood lignin that has undergone several kinds of treatment, leads to a complex mixture of LMW compounds, making the separation of components for the formation of value-added chemicals more difficult. Identifying the phenolic compounds in the LMW fractions obtained under different depolymerization conditions is essential for the upgrading process. In this study, a state-of-the-art nontargeted analysis method using ultra-high-performance supercritical fluid chromatography coupled to high-resolution multiple-stage tandem mass spectrometry (UHPSFC/HRMSn) combined with a Kendrick mass defect-based classification model was applied to analyze the monomers and oligomers in the LMW fractions separated from BLR samples depolymerized at 170–210 °C. The most common phenolic compound types were dimers, followed by monomers. A second round of depolymerization yielded low amounts of monomers and dimers, while a high number of trimers were formed, thought to be the result of repolymerization.

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