ANTIOXIDANT ACTIVITY OF FOREST MANGGOSTEEN  (Garcinia hombroniana Pierre) FRACTION USING DPPH AND ABTS METHOD

This study was carried out to determine the active antioxidant fraction of Garcinia hombroniana bark extract by the DPPH and ABTS method. Along with this, the study also attempts to identify the class of compounds present in the various extract of G. hombroniana by the active fraction. The bark extract of G. hombroniana was prepared by the multilevel maceration method by using three solvents including n-hexane, ethyl acetate, and 96% ethanol. Results of study suggested that n-hexane (HSE), ethyl acetate (EASE) and ethanol 96% extract (ESE) have antioxidant activity with IC50 value of 423 ± 16.72 μg/mL, 284.89 ± 2.7μg/mL, and 10.49 ± 0.19 μg/mL in DPPH assay, while these extracts showed IC50 value of 560.92 ± 48.86 μg/mL, 430.18 ± 16.65 μg/mL, and 13.92 ± 0.57 μg/mL respectively in ABTS assay. Further, fractionated using vacuum column chromatography revealed the presence of five fractions viz., A, B, C, D, and E. Among these, fractions D showed the highest antioxidant activity with an IC50 value of 4.83 ± 0.18 μg/mL and 6.82 ± 0.31 μg/mL in DPPH and ABTS assays. Results of the fractioned analysis and qualitative determination showed that the active fraction of G. hombroniana contained flavonoid, triterpenoid, alkaloid, and saponin compounds, and antioxidant activities of these extracts might be due to the presence of these active ingredients.

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Antioxidant Activity of Syzygium polyanthum Extracts

Indonesian Journal of Chemistry ◽

10.22146/ijc.23545 ◽

2017 ◽

Vol 17 (1) ◽

pp. 49 ◽

Cited By ~ 8

Author(s):

Mutia Devi Hidayati ◽

Taslim Ersam ◽

Kuniyoshi Shimizu ◽

Sri Fatmawati

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Sulfonic Acid ◽

Methanol Extract ◽

Antioxidant Activities ◽

Dpph Assay ◽

Abts Assay ◽

Ic50 Value

Antioxidant activities of Syzygium polyanthum leaves extracts (methanol, ethyl acetate, dichloromethane and n-hexane) were evaluated by using DPPH (2,2-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azinobis (3-ethylbenzothiazole-6-sulfonic acid) methods. The methanol extract showed the highest antioxidant activity of DPPH assay among extract with IC50 value of 44.35 mg/mL. In addition, methanol extract also showed the highest antioxidant activity of ABTS assay among extracts with IC50 value of 17.69 mg/mL. This study indicated that the methanol extract of S. polyanthum leaves is potential as antioxidant.

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UJI AKTIVITAS ANTIOKSIDAN BEBERAPA EKSTRAK ALGA COKLAT Sargassum polycystum DAN Turbinaria deccurens ASAL PULAU DUTUNGAN SULAWESI SELATAN TERHADAP RADIKAL DPPH

Jurnal Kimia Riset ◽

10.20473/jkr.v4i1.10903 ◽

2019 ◽

Vol 4 (1) ◽

pp. 1

Author(s):

Fitriyanti Jumaetri Sami ◽

Nunuk Hariani Soekamto ◽

Firdaus Firdaus ◽

Jalifah Latip

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Antioxidant Vitamin ◽

Activity Assay ◽

Immersion Method ◽

Ic50 Value ◽

Measured Absorption ◽

Hexane Extracts

This study used S. polycystum and T. deccurens were extracted with various solvents (n-hexane, ethyl acetate and ethanol) to determine phytochemical screening and antioxidant activities. Extraction of samples by maceration method, antioxidant activity assay using the immersion method of DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 515 nm and compared with the control of antioxidant vitamin C. The results showed that antioxidant activity of the methanol, ethyl acetate, n-hexane extract of S. polycystum have low antioxidant activity with the value IC50 340,06, 180,54, and 502,25 mg/mL. Methanol, ethyl acetate, n-hexane extracts of T. deccurens have low antioxidant activity with value IC50 491,02, 411,80, and 502,7 mg/mL. This potential is lower than vitamin C IC50 value of 1,72 mg/mL.

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ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK

Jurnal Natural ◽

10.24815/jn.v19i3.12618 ◽

2019 ◽

Vol 19 (3) ◽

pp. 58-63

Author(s):

Ilham Maulana ◽

Binawati Ginting ◽

Nurdin Nurdin ◽

Saiful Fakri

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Stem Bark ◽

Bark Extract ◽

Test Results ◽

Myristica Fragrans ◽

Ic50 Value ◽

Stem Bark Extract ◽

Myristica Fragrans Houtt

Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.

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Antioxidant Activity of Multifarious Solvent Extracts of Leaf. (Plumbago Zeylanica)

Oriental Journal Of Chemistry ◽

10.13005/ojc/350230 ◽

2019 ◽

Vol 35 (2) ◽

pp. 718-722

Author(s):

D. Roselin Jenifer ◽

B. R. Malathy

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Reducing Power ◽

Chloroform Extract ◽

Assay Method ◽

Dpph Assay ◽

Leaf Extracts ◽

Plumbago Zeylanica ◽

Reducing Power Assay

The current investigation is focused to determine antioxidant activities of the leaf extract of Plumbago zeylanica. The leaf extracts were prepared by using multiform solvents with increasing polarity, including as hexane, chloroform, ethyl acetate and Methanol. The antioxidant activities enlisting DPPH assay, hydrogen peroxide assay, reducing power assay and nitric oxide assay method were performed for all extracts. Barring no one, the extracts had shown average to potent antioxidant activity. Uniquely, the ethyl acetate extract and the chloroform extract explicated the utmost antioxidant activity.

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Antioxidant Activities of n-Hexane Soluble and Insoluble Fraction, Ethyl Acetate Soluble and Insoluble Fraction from Ethanol Extract of Sambung Nyawa Leaf (Gynura procumbens (Lour.) Merr.)

Majalah Obat Tradisional ◽

10.22146/mot.43743 ◽

2019 ◽

Vol 24 (2) ◽

pp. 91

Author(s):

Purwantiningsih Purwantiningsih ◽

Retno Murwanti ◽

Lukman Hakim

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Ethyl Acetate ◽

Soluble Fraction ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Ethanol Extract ◽

Insoluble Fraction ◽

Ic50 Value ◽

Antioxidant Potency

There were many plants containing flavonoids which can function as antioxidant. Antioxidant can be used to decrease the mortality rate caused by degenerative and infectious diseases. This study aims to determine the antioxidant potency of n-hexane soluble and insoluble fraction, ethyl acetate soluble and insoluble fraction, and the ethanol extract antioxidant activity of Sambung Nyawa leaf (Gynura procumbens (Lour.) Merr.). The ethanol extract of G. procumbens leaves was obtained by maceration using 70% ethanol then fractionated using hexane and ethyl acetate to obtain the n-hexane soluble fraction, n-hexane insoluble fraction, ethyl acetate soluble fraction, and ethyl acetate insoluble fraction. The antioxidant activity study of ethanol extract, n-hexane soluble, and insoluble fraction, ethyl acetate soluble, and insoluble fraction of G. procumbens was carried out using DPPH (2,2- diphenyl-1-picrylhydrazyl) method to evaluate its free radical scavenging activity with a comparison of vitamin C. The antioxidants activity was expressed as IC50 value. The IC50 value for vitamin C and ethanol extract of G. procumbens leaves were 2.41 µg/ml and 200 µg/ml. On the other hand, the n-hexane soluble and insoluble fraction had an IC50 value of 78 µg/ml and 34 µg/ml, while ethyl acetate soluble and insoluble fraction had the potency as an antioxidant with an IC50 value of 419 µg/ml and 151 μg/ml, respectively. Based on the results obtained, the antioxidant potency of G. procumbens ethanol extract and their fraction were successively of n-hexane insoluble fraction > n-hexane soluble fraction > ethyl acetate insoluble fraction > ethanol extract > ethyl acetate soluble fraction.

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FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KULIT BATANG Chisocheton sp. (C.DC) Harms (Meliaceae)

CHEMISTRY PROGRESS ◽

10.35799/cp.13.2.2020.31672 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Dewa Gede Katja

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Extract ◽

Stem Bark ◽

Bark Extract ◽

Test Results ◽

Ic50 Value ◽

Dpph Method ◽

Stem Bark Extract

Tujuan penelitian ini untuk mengetahui golongan senyawa metabolit sekunder dan aktivitas antioksidan dari setiap ekstrak kulit batang Chisocheton sp. C.DC Harms (Meliaceae). Hasil ekstraksi 200 g serbuk kulit batang Chisocheton sp. C.DC Harms (Meliaceae) dengan n-heksana, etil asetat dan metanol masing-masing dengan 2000 mL berturut-turut menghasilkan 7,193 g ekstrak pekat n-heksana, 8,798 g ekstrak pekat etil asetat dan 18,683 g ekstrak pekat metanol. Hasil uji fitokimia menunjukkan adanya golongan senyawa flavonoid, triterpenoid dan tanin. Hasil uji aktivitas antioksidan dengan metode DPPH menunjukkan bahwa ekstrak n-heksana memberikan nilai IC50 sebesar 337,28 µg/mL, ekstrak metanol sebesar 216,73 µg/mL, dan ekstrak etil asetat sebesar 199,89 µg/mL yang berarti etil asetat memiliki kemampuan yang paling besar dalam menangkap radikal bebas.ABSTRACTThe objective of this study was determine the class of secondary metabolite compounds and antioxidant activity of each stem bark extract of Chisocheton sp. C.DC Harms (Meliaceae). The results of the extraction of 200 g Chisocheton sp. C.DC Harms (Meliaceae) with n-hexane, ethyl acetate and methanol with 2000 mL each yielded 7,193 g of concentrated extract n-hexane, 8,798 g of concentrated extract of ethyl acetate and 18,683 g of concentrated methanol extract. Phytochemical test results showed the presence of flavonoid, triterpenoid and tannin compounds. The results of the antioxidant activity test using the DPPH method showed that the n-hexane extract gave an IC50 value of 337,28 µg/mL, methanol extract was 216,73 µg/mL, and ethyl acetate extract was 199,89 µg/mL which means that ethyl acetate has the ability to greatest in capturing free radicals.

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Evaluation of Antioxidant Activities From Ethyl Acetate Fraction of Curry Leaf Using DPPH Method

Elkawnie ◽

10.22373/ekw.v7i1.8059 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Ulil Amna ◽

Halimatussakdiah Halimatussakdiah ◽

Furqan Nur Ihsan ◽

Puji Wahyuningsih

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Ethyl Acetate Fraction ◽

Murraya Koenigii ◽

Chromatography Method ◽

Ic50 Value ◽

Dpph Method ◽

Ethyl Acetate Extracts

Abstract: Curry Plant (Murraya koenigii (Linn.) Spreng) is one of the plants that thrive in Indonesia. Curry contains secondary metabolites of alkaloids and flavonoids that have the potential as antioxidants. Antioxidants can reduce the activity of free radicals that can cause degenerative diseases such as cancer, coronary heart disease, and premature ageing of body cells by donating the protons to free radical compounds. This study aimed to determine the antioxidant activity and identify the active compound class of ethyl acetate fraction from curry leaves. Separation of secondary metabolites of ethyl acetate extracts was carried out by column chromatography method, which obtained 5 different fractions. The antioxidant activity of fractions A, B, C, D, and E was tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with variations concentrations of 25, 50, 100, 200, and 400 ppm, and ascorbic acid was used as a comparison which measured at a wavelength of 517 nm. The results showed that fractions A and E have weak antioxidant activities, while fractions B, C, and D showed moderate antioxidant activities. The highest antioxidant activity was found in fraction B with an IC50 value of 341.38 ppm, where the fraction showed the presence of secondary metabolites of alkaloids and terpenoids.Abstrak: Tanaman kari ((Murraya koenigii (Linn.) Spreng) merupakan salah satu tanaman yang tersebar di Indonesia. Kari mengandung senyawa metabolit sekunder alkaloid dan flavonoid yang memiliki potensi sebagai antioksidan. Antioksidan dapat mengurangi aktivitas radikal bebas yang dapat menyebabkan penyakit degeneratif seperti kanker, penyakit jantung coroner dan penuaan sel dengan cara menyumbangkan proton pada senyawa radikal bebas. Tujuan dari penelitian ini adalah untuk menentukan aktivitas antioksidan dan mengidentifikasi kelas senyawa aktif fraksi etil asetat dari daun kari. Pemisahan metabolit sekunder dari ekstrak etil asetat dilakukan menggunakan metode kromatografi kolom dengan menghasilkan 5 fraksi yang berbeda. Aktivitas antioksidan dari fraksi A, B, C ,D ,dan E dilakukan menggunakan metode DPPH (1,1-diphenil-2-pikrilhydrazil) dengan variasi konsentrasi 25, 50, 100, 200, dan 400 ppm dan asam askorbat digunakan sebagai perbandingan yang diukur pada panjang gelombang 517 nm. Hasil menunjukkan bahwa fraksi A dan E memiliki aktivitas antioksidan yang lemah, sedangkan fraksi B, C, dan D menunjukkan aktivitas antioksidan sedang. Aktivitas antioksidan yang paling tinggi ditemukan pada fraksi B dengan IC50 341,38 ppm. Fraksi tersebut menunjukkan adanya kandungan senyawa metabolit sekunder alkaloid dan terpenoid.

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ANTIOXIDANT ACTIVITY OF RED ROSE LEAVES (Rosa chinensis Jacq.) EXTRACT

Acta Biochimica Indonesiana ◽

10.32889/actabioina.v3i2.56 ◽

2021 ◽

Vol 3 (2) ◽

pp. 81-88

Author(s):

Deviyanthi Nur Afifah ◽

Yessy Yessy ◽

Farida Nur Rahmani ◽

Ayu Lia Ningsih ◽

Citra Dewi Puspita Sari ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Activity Index ◽

Radical Reactions ◽

Dpph Assay ◽

Free Radical Reactions ◽

Rosa Chinensis ◽

Ic50 Value ◽

Hexane Extracts

Background: Background: Antioxidants are compounds that can neutralize free radical reactions. The red rose leaves (Rosa chinensis Jacq.), usually disposed of as waste, have been shown to have antioxidant activity. Objective: This study aims to determine the antioxidant activity of red rose leaves extracts. Methods: The red rose leaves were extracted by the maceration method in ethanol, methanol, ethyl acetate, and n-hexane. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and was expressed as IC50 and an antioxidant activity index (AAI).Results: The results showed that the red rose leaves ethanol, methanol, ethyl acetate, and n-hexane extracts had an antioxidant activity with an IC50 value of 1.8, 8.7, 210.3, 48.7 µg/mL, respectively, and an AAI value of 17.3, 3.5, 0.1, 0.6, respectively. The main content of ethanol and methanol extracts are flavonoid, saponin, and tannin.Conclusion: The ethanol and methanol extract showed the most potent antioxidant activity.

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Chemical Composition, Bioactive Components and Antioxidant Activities from Root, Bark and Leaf Lindur

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v18i2.10614 ◽

2015 ◽

Vol 18 (2) ◽

Author(s):

Siluh Putu Sri Dia ◽

N. Nurjanah ◽

Agoes Mardiono Jacoeb

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Bark Extract ◽

Root Extract ◽

Root Bark ◽

Bioactive Components ◽

Bruguiera Gymnorrhiza ◽

Ethyl Acetate Extracts

Plants lindur (Bruguiera gymnorrhiza) is one of the plants that have a potential as a source of bioactive compounds for the antioxidant. This study aimed to determine the chemical composition, bioactive compound and determine antioxidant activity of leaves, bark and roots of plants lindur (B. gymnorrhiza). This research was conducted through several stages proximate test, stratified extraction, phytochemical test, and antioxidant activity test with DPPH. The highest yield value resulting by ethanol extract of the leaves is 12,85 % and the lowest was produced by n-hexane root extract that is 0,18 %. The leaves contain high levels of protein, fat, and water which is the higher of the two parts from the other samples. Bark containing ash content (4,12%) and carbohydrates (46,02%). Root has the highest levels of carbohydrates that is 25,91 %. Bioactive components were detected on ethanol and ethyl acetate extracts of the leaves are flavonoids, tannins, phenols, saponins, steroids and tritepenoid. Bark extract ethanol, ethyl acetate and ethanol root bark has bioactive components there are flavonoids, tannins, phenols, saponins, and tritepenoid. Ethyl acetate extract of the roots only have bioactive components there are flavonoids, phenols, saponins, and triterpenoids. The potential antioxidant activity produced from ethyl acetate extracts of bark lindur with IC50 values is 14,21 ppm. Keywords: Antioxidants, phytochemicals, roots, ethanol, Bruguiera gymnorrhiza

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Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

Current Biochemistry ◽

10.29244/cb.5.3.11-20 ◽

2019 ◽

Vol 5 (3) ◽

pp. 11-20

Author(s):

Stefani Dhale Rale ◽

Hasim Hasim ◽

Syamsul Falah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Inhibition Activity ◽

Active Compounds ◽

Activity Inhibition ◽

Activity Test ◽

Ic50 Value ◽

Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

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