Prediction of forage digestibility from some laboratory procedures. 2. Comparison of in vivo digestibility at two institutes.

1971 ◽  
Vol 19 (2) ◽  
pp. 106-113
Author(s):  
B. Deinum
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Sulfuric Acid ◽  
Lignin Content ◽  
Forage Digestibility ◽  
Significant Difference ◽  
Laboratory Procedures ◽  
Sulfuric Acid Lignin

For part 1 see Abst. 1564, Vol. 40. 2. The relation between digestibilities of organic matter in vivo and in vitro did not differ significantly between the institutes at Hoorn and Wageningen. No difference was found in digestibility of the cell wall constituents in relation to the lignin content, nor was there a significant difference in the relation between the percentage of digestible cell contents and the percentage of cell contents in forage from well managed pastures. Twelve forages of highly digestible perennial ryegrass deviated from these farm forages at Wageningen, showing smaller excretion of bacterial and endogenous residue. Comparison of the 2 lignin procedures showed that 72% sulfuric acid lignin gave consistent residual standard deviations of digestibility of cell wall constituents; permanganate lignin gave smaller errors in forages at Wageningen but greater errors in forages at Hoorn. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Prediction of forage digestibility from some laboratory procedures.

1969 ◽  
Vol 17 (2) ◽  
pp. 119-127
Author(s):  
B. Duinum ◽  
P.J. Van Soest
Keyword(s):  
Cell Wall ◽  
Chemical Analysis ◽  
Lignin Content ◽  
Rumen Fluid ◽  
Grass Silage ◽  
Forage Digestibility ◽  
The Poor ◽  
Laboratory Procedures

Digestibility of 106 samples of hay, grass, grass silage and legume forage was estimated by regression from cell contents and cell wall constituents estimated chemically. Cell wall constituents were cellulose, hemicelluloses, lignin, cutin, plant silica and soil contamination. Values were not as closely related to digestibility in vivo by sheep as were values obtained by digestion with rumen fluid in vitro. Correlation between digestibility of cell wall constituents and lignin content was poor, and this and other reasons for the poor results from chemical analysis are discussed.-T. D. B. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals The in vivo impact of MsLAC1, a Miscanthus laccase isoform, on lignification and lignin composition contrasts with its in vitro substrate preference

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Feng He ◽  
Katja Machemer-Noonan ◽  
Philippe Golfier ◽  
Faride Unda ◽  
Johanna Dechert ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factors ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Lignin Composition ◽  
Xylem Fibers ◽  
Breeding Target

Abstract Background Understanding lignin biosynthesis and composition is of central importance for sustainable bioenergy and biomaterials production. Species of the genus Miscanthus have emerged as promising bioenergy crop due to their rapid growth and modest nutrient requirements. However, lignin polymerization in Miscanthus is poorly understood. It was previously shown that plant laccases are phenol oxidases that have multiple functions in plant, one of which is the polymerization of monolignols. Herein, we link a newly discovered Miscanthus laccase, MsLAC1, to cell wall lignification. Characterization of recombinant MsLAC1 and Arabidopsis transgenic plants expressing MsLAC1 were carried out to understand the function of MsLAC1 both in vitro and in vivo. Results Using a comprehensive suite of molecular, biochemical and histochemical analyses, we show that MsLAC1 localizes to cell walls and identify Miscanthus transcription factors capable of regulating MsLAC1 expression. In addition, MsLAC1 complements the Arabidopsis lac4–2 lac17 mutant and recombinant MsLAC1 is able to oxidize monolignol in vitro. Transgenic Arabidopsis plants over-expressing MsLAC1 show higher G-lignin content, although recombinant MsLAC1 seemed to prefer sinapyl alcohol as substrate. Conclusions In summary, our results suggest that MsLAC1 is regulated by secondary cell wall MYB transcription factors and is involved in lignification of xylem fibers. This report identifies MsLAC1 as a promising breeding target in Miscanthus for biofuel and biomaterial applications.

The use of cell wall organic matter components and in vitro degradability characteristics to predict intake and digestibility of white clover for sheep

1995 ◽  
Vol 46 (6) ◽  
pp. 1111 ◽  
Author(s):  
KS Nandra ◽  
VH Oddy ◽  
JF Ayres ◽  
PJ Nicholls ◽  
B Langevad ◽  
...  
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
White Clover ◽  
Good Precision ◽  
Digestible Organic Matter ◽  
Predictive Relations ◽  
Voluntary Intake ◽  
Organic Matter Digestibility

The relations of the laboratory measurement of cell wall organic matter (CWOM) components and of the in vitro degradability characteristics of CWOM with in vivo digestibility and voluntary intake for high quality white clover were investigated. The voluntary intake, apparent digestibility and apparent rumen retention time of CWOM of white clover harvested at various stages of maturity were measured in rumen-cannulated Merino wether sheep. The in vitro degradability characteristics of CWOM of these diets were also measured. This study has quantified strong predictive relations between structural fibre constituents or degradation parameters and both digestibility and intake for white clover. The CWOM, cellulose and hemicellulose contents and potential degradability of CWOM of the white clover predicted in vivo organic matter digestibility with good precision (r2 = 0.74, 0.67, 0.72 and 0.72 respectively). The voluntary intakes of organic matter and digestible organic matter of the white clover were strongly related to CWOM, cellulose and hemicellulose contents and to the rate of degradation of CWOM in the rumen and the fits of these relations were at least as good as those predicting organic matter digestibility. The predictive equations based on CWOM: OMD (g/kg) = 97.6-0.081 (� 0.012) CWOM OMI (g/kg) = 83-0.085 (� 0.018) CWOM DOMI (g/kg) = 71.4-0.098 (� 0.015) CWOM are recommended because of the ease of analysis of CWOM in the laboratory.

Nutritive value of the olive leaf: effects of cultivar, season of harvesting and system of drying

1992 ◽  
Vol 119 (2) ◽  
pp. 205-210 ◽  
Author(s):  
A. Cabrera-Gomez ◽  
A. Garrido ◽  
J. E. Guerrero ◽  
V. Ortiz
Keyword(s):  
Organic Matter ◽  
Chemical Composition ◽  
Nutritive Value ◽  
Drying Procedures ◽  
Significant Difference ◽  
Different Seasons ◽  
Organic Matter Digestibility ◽  
Olive Trees

SUMMARYLeaves of olive trees (Olea europaea L.) of eight cultivars, Manzanilla, Picual, Hojiblanca, Edremit, Conservolia, Picholine-Marroqui, Chemlali and Frantoio, were collected in different seasons and years in Córdoba, Spain. Their chemical composition and in vitro organic matter digestibility values were determined. Differences in composition and nutritive value between cultivars, years and seasons were studied in Manzanilla, Picual and Hojiblanca, the three most important local cultivars. There were significant differences both between cultivars and seasons (P < 0·05) and a non-significant difference between years.The effect of various drying procedures (fresh, oven-dried, air-dried indoors or air-dried outdoors) on chemical composition, in vitro digestibility and in vivo digestibility in sheep was also examined. The in vivo organic matter digestibility of fresh leaves was significantly higher than that of dried leaves. No difference was found between leaves air-dried indoors and oven-dried leaves. However, leaves air-dried outdoors were significantly less digestible. Quick drying in an oven reduced the digestibility of crude protein and acid detergent fibres compared to air drying indoors. There was a significant decrease in digestibility of 11% when leaves were dried separated from the branches as compared to leaves dried on the branches.

The constancy of the digested cell wall in grass

1974 ◽  
Vol 83 (2) ◽  
pp. 295-298 ◽  
Author(s):  
K. W. Moir
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Nitrogen Fertilizer ◽  
Total Cell ◽  
Digitaria Decumbens ◽  
Different Levels

SUMMARYThe quantity of in vitro digested cell wall was determined in the separated leaf and stem of Digitaria decumbens (pangola grass) fertilized at three different levels of nitrogen and cut after two different periods of regrowth. The treatment means ranged from 39·6 to 41·5 g digested cell wall per 100 g forage organic matter in leaf, and from 37·5 to 41·6 g in stem. For both leaf and stem the lowest mean amounts of digested cell wall were associated with the highest levels of fertilizer and the longer periods of regrowth. The results for nitrogen fertilizer were consistent with those from previous in vivo digestibility experiments, but when the effect of the total cell wall on digested cell wall was taken into account there were inconsistencies. The previous in vivo digestibility data were used in conjunction with the present results in support of a view that interpretation of digestibility data for grasses is best made by reference to a constant digested cell wall of 39·5 g/100 g forage organic matter.

The relationship between in vitro digestible cell wall and the cell-wall content of forage

1973 ◽  
Vol 81 (3) ◽  
pp. 533-536 ◽  
Author(s):  
K. W. Moir
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Panicum Maximum ◽  
Average Value ◽  
Whole Plant ◽  
Whole Plants ◽  
The Relationship

SUMMARYIn oven-dried whole plants from three regrowths of four varieties of Panicum maximum, leaf and stem of normal and stunted oats at three stages of first growth, leaf and stem of rape and the whole plant of millet, cell wall ranged from 14 to 77% of the organic matter. In vitro digestible cell wall ranged from 13 to 46% of the organic matter and was closely related to the cell wall (residual standard deviation, ± 2·5). In 16 legume hays the average in vitro digestible cell wall was 22·1% of the forage organic matter compared with an average value of 20·5% found previously from in vivo digestibility experiments with legumes. An unsatisfactory feature of the in vitro digestibility determination was that in silages and low quality grass hays, the digestible cell wall was low relative to known in vivo values for these forage types. The separate determinations of cell wall and in vitro digestible cell wall would add to the confidence that can be placed on estimates of in vivo digestibility from laboratory measurements. Often, the determination of cell-wall content is sufficient for this purpose.

The digestibility of wheat straw treated with sulphur dioxide

1984 ◽  
Vol 102 (3) ◽  
pp. 517-520 ◽  
Author(s):  
D. Ben-Ghedalia ◽  
J. Miron
Keyword(s):  
Cell Wall ◽  
Organic Matter ◽  
Wheat Straw ◽  
Sulphur Dioxide ◽  
Soluble Fraction ◽  
Apparent Digestibility

SummaryTwo digestion trials were performed with sheep in order to assess the effect of the sulphur dioxide treatment on the digestibility of wheat straw in vivo. In the first trial, untreated and SO2-treated straw served as the major components of the diets. Sulphur dioxide partly solubilized the cell wall (CW) material of the straw, decreasing its content from 78·5 to 55·9%. The digestibility of the residual CW was increased from 49·2 to 77·8%, whereas the digestibility values of the cell-soluble fraction (CS) were unaffected by the treatment: 55·8 and 57·8% for the untreated and treated straw, respectively. Apparent digestibility of the organic matter (OM) was increased by the treatment from 46 to 65%.In the second trial SO2-treated wheat straw was fed together with concentrates at a D.M. ratio of 30/70, and the digestibility of the OM and CW was determined.CW and OM digestibilities of the treated straw were not affected by 70% concentrate in the diet and the respective values were 77·0 and 65·5%, very similar to those found in the first trial. The reason for the in vitro–in vivo discrepancy is discussed.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

scholarly journals The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

2017 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Mulyati Mulyati ◽  
Suryati Suryati ◽  
Irfani Baga
Keyword(s):  
Survival Rate ◽  
Sea Water ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Inhibitory Ability ◽  
Significant Difference ◽  
Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

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