scholarly journals Therapeutic applications of stem cells from human exfoliated deciduous teeth: a review

2020 ◽  
Vol 8 (9) ◽  
pp. 3413
Author(s):  
Mani Baweja
Keyword(s):  
Stem Cells ◽  
Animal Studies ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Oral Diseases ◽  
Dental Stem Cells ◽  
Medline Search ◽  
Therapeutic Applications ◽  
Human Exfoliated Deciduous Teeth

Dental stem cells have been found to have the ability to differentiate into nerve cells, adipose cells, chondrocytes, osteoblasts, myocytes, hepatocytes, and odontoblasts. They can be derived from permanent teeth or deciduous teeth. Stem cells from human exfoliated deciduous teeth (SHED) have a higher proliferation rate and higher osteogenic and neurogenic potential than dental pulp stem cells (DPSC). Therefore, SHEDs are an attractive cell source for tissue regeneration. A large plethora of in vitro and animal studies have been conducted in the last few decades that has demonstrated the potential uses of these cells for the treatment of oral and non-oral diseases. The aim of this article was to review the potential therapeutic applications of stem cells derived from human exfoliated deciduous teeth. A Medline search was done, including international literature, published in English between 2003 and 2020. In this area, further research is needed to ensure the applicability of SHED in the treatment of diseases in humans.

Stem Cells Derived from Human Exfoliated Deciduous teeth [shed] in Neuronal Disorders: A Review

2020 ◽  
Vol 16 ◽  
Author(s):  
Minu Anoop ◽  
Indrani Datta
Keyword(s):  
Stem Cells ◽  
Neurodegenerative Diseases ◽  
Dental Pulp ◽  
Therapeutic Potential ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Proliferative Potential ◽  
Pulp Tissue ◽  
Human Exfoliated Deciduous Teeth

: Most conventional treatments for neurodegenerative diseases fail due to their focus on neuroprotection rather than neurorestoration. Stem cell‐based therapies are becoming a potential treatment option for neurodegenerative diseases as they can home in, engraft, differentiate and produce factors for CNS recovery. Stem cells derived from human dental pulp tissue differ from other sources of mesenchymal stem cells due to their embryonic neural crest origin and neurotrophic property. These include both dental pulp stem cells [DPSCs] from dental pulp tissues of human permanent teeth and stem cells from human exfoliated deciduous teeth [SHED]. SHED offer many advantages over other types of MSCs such as good proliferative potential, minimal invasive procurement, neuronal differentiation and neurotrophic capacity, and negligible ethical concerns. The therapeutic potential of SHED is attributed to the paracrine action of extracellularly released secreted factors, specifically the secretome, of which exosomes is a key component. SHED and its conditioned media can be effective in neurodegeneration through multiple mechanisms, including cell replacement, paracrine effects, angiogenesis, synaptogenesis, immunomodulation, and apoptosis inhibition, and SHED exosomes offer an ideal refined bed-to-bench formulation in neurodegenerative disorders. However, in spite of these advantages, there are still some limitations of SHED exosome therapy, such as the effectiveness of long-term storage of SHED and their exosomes, the development of a robust GMP-grade manufacturing protocol, optimization of the route of administration, and evaluation of the efficacy and safety in humans. In this review, we have addressed the isolation, collection and properties of SHED along with its therapeutic potential on in vitro and in vivo neuronal disorder models as evident from the published literature.

In vitro and in vivo characteristics of stem cells from human exfoliated deciduous teeth obtained by enzymatic disaggregation and outgrowth

2014 ◽  
Vol 59 (10) ◽  
pp. 1013-1023 ◽  
Author(s):  
Mijeong Jeon ◽  
Je Seon Song ◽  
Byung-Jai Choi ◽  
Hyung-Jun Choi ◽  
Dong-Min Shin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Deciduous Teeth ◽  
Human Exfoliated Deciduous Teeth

Comparison of human dental follicle cells (DFCs) and stem cells from human exfoliated deciduous teeth (SHED) after neural differentiation in vitro

2009 ◽  
Vol 14 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Christian Morsczeck ◽  
Florian Völlner ◽  
Michael Saugspier ◽  
Caroline Brandl ◽  
Torsten Eugen Reichert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Differentiation ◽  
Follicle Cells ◽  
Deciduous Teeth ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Human Exfoliated Deciduous Teeth

Stem Cells from Human Exfoliated Deciduous Teeth: A Growing Literature

2016 ◽  
Vol 202 (5-6) ◽  
pp. 269-280 ◽  
Author(s):  
Daniel Martinez Saez ◽  
Robson Tetsuo Sasaki ◽  
Adriana da Costa Neves ◽  
Marcelo Cavenaghi Pereira da Silva
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Adult Stem Cells ◽  
Current Knowledge ◽  
Deciduous Teeth ◽  
Easy Access ◽  
Stem Cell Population ◽  
Scientific Publications ◽  
Human Exfoliated Deciduous Teeth

Adult stem cells research has been considered the most advanced sort of medical-scientific research, particularly stem cells from human exfoliated deciduous teeth (SHED), which represent an immature stem cell population. The purpose of this review is to describe the current knowledge concerning SHED from full-text scientific publications from 2003 to 2015, available in English language and based on the keyword and/or abbreviations ‘stem cells from human exfoliated deciduous teeth (SHED)', and individually presented as to the properties of SHED, immunomodulatory properties of SHED and stem cell banking. In summary, these cell populations are easily accessible by noninvasive procedures and can be isolated, cultured and expanded in vitro, successfully differentiated in vitro and in vivo into odontoblasts, osteoblasts, chondrocytes, adipocytes and neural cells, and present low immune reactions or rejection following SHED transplantation. Furthermore, SHED are able to remain undifferentiated and stable after long-term cryopreservation. In conclusion, the high proliferative capacity, easy access, multilineage differentiation capacity, noninvasiveness and few ethical concerns make stem cells from human exfoliated deciduous teeth the most valuable source of stem cells for tissue engineering and cell-based regenerative medicine therapies.

scholarly journals Isolation and culture of dental pulp stem cells from permanent and deciduous teeth

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Shagufta Naz ◽  
Farhan Raza Khan ◽  
Raheela Rahmat Zohra ◽  
Sahreena Salim Lakhundi ◽  
Mehwish Sagheer Khan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Tooth Pulp ◽  
Human Teeth ◽  
Creative Commons ◽  
Calcified Tissue

Objective: To isolate dental pulp mesenchymal stem cells (MSCs) from non-infected human permanent and deciduous teeth. Methods: It was an in-vitro experimental study. Human teeth were collected from 13 apparently healthy subjects including nine adults and four children. After decoronation dental pulps were extirpated from teeth and cultured via explant method in a stem cell defined media. Data was analyzed by descriptive statistics. Results: As above MSCs emerged exhibiting fibroblast-like morphology. In vitro culture was positive for 100% (9/9) and 75% (3/4) of the permanent and deciduous teeth respectively. First cell appeared from deciduous teeth pulp in 10±6.2 days while permanent teeth pulp took 12.4±3.7 days. Together, 26.6±3.6 and 24.5±3.5 days were required for permanent and deciduous tooth pulp stem cells to be ready for further assays. Conclusions: The protocol we developed is easy and consistent and can be used to generate reliable source of MScs for engineering of calcified and non-calcified tissue for regenerative medicine approaches. doi: https://doi.org/10.12669/pjms.35.4.540 How to cite this:Naz S, Khan FR, Zohra RR, Lakhundi SS, Khan MS, Mohammed N, et al. Isolation and culture of dental pulp stem cells from permanent and deciduous teeth. Pak J Med Sci. 2019;35(4):---------. doi: https://doi.org/10.12669/pjms.35.4.540 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

scholarly journals In vitro histomorphometric comparison of dental pulp tissue in different teeth

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8212
Author(s):  
Marytere Guerrero-Jiménez ◽  
Geovanny I. Nic-Can ◽  
Nelly Castro-Linares ◽  
Fernando Javier Aguilar-Ayala ◽  
Michel Canul-Chan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Density ◽  
Dental Pulp ◽  
Pearson Correlation ◽  
Third Molar ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Pulp Tissue ◽  
Valuable Source

Background Dental pulp (DP) represents an accessible and valuable source promising of stem cells for clinical application. However, there are some disadvantages associated with the isolation of dental pulp stem cells (DPSCs), which include the size and weight of the pulp tissue needed to yield sufficient cells for culturing in vitro. Therefore, the objective of this study was to compare in vitro histomorphometry of DP from permanent (premolars, third molar), supernumerary and deciduous teeth of patients between 5 and 25 years old with regards to weight, length, width and the cell density in the four regions of the DP in order to obtain quantitative parameters in a tissue that represents a valuable source of stem cells. Methods DPs were obtained from 10 central incisors deciduous, 20 permanent teeth (10 premolars, 10 third molars) and 10 supernumeraries (six mesiodents and four inferior premolar shapes). The pulps were carefully removed, and the entire tissue was weighed. The pulp length and the width were measured with a digital Vernier caliper. The cellular density analysis was performed according to the four regions of the DP (coronal, cervical, medial and apical) in histological slides using photography and the ImageJ® program for quantification. Results The Pearson correlation test revealed that DP weight among different types of teeth is correlated with age in male patients. A significant positive correlation was noted between length and width of the DP with age in both genders. The mean DP weight for supernumerary and third molar teeth was greater than deciduous and premolar teeth. Finally, the histological analysis showed that the coronal and apical portions of DP in supernumerary and premolar teeth have the highest cell density. Conclusions The DP of supernumerary teeth has quantitatively the best morphometric parameters and cell density comparable with the quality of DP obtained from deciduous teeth.

scholarly journals Dental stem cells and their application in Dentistry: a literature review

2016 ◽  
Vol 73 (4) ◽  
pp. 331 ◽  
Author(s):  
Paula Nascimento Almeida ◽  
Karin Soares Cunha
Keyword(s):  
Stem Cells ◽  
Literature Review ◽  
Bone Repair ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Dental Stem Cells ◽  
Dental Origin ◽  
Apical Papilla ◽  
Regenerative Therapies ◽  
Interesting Alternative

Objective: the aim of this study was to conduct a literature review of the types of stem cells of dental origin and their applications in Dentistry. Material and Methods: for this, we selected scientific articles published between 2000 and 2016 through the databases PUBMED and LILACS. Results: there are five main sources of stem cells of dental origin: stem cells from dental pulp of permanent teeth and deciduous teeth, apical papilla, periodontal ligament and dental follicle. These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry.

scholarly journals Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application

2016 ◽  
Vol 2016 ◽  
pp. 1-20 ◽  
Author(s):  
Athina Bakopoulou ◽  
Imad About
Keyword(s):  
Stem Cells ◽  
Well Being ◽  
Biological Properties ◽  
Deciduous Teeth ◽  
Regenerative Dentistry ◽  
Dental Origin ◽  
Apical Papilla ◽  
Human Exfoliated Deciduous Teeth

Dental Mesenchymal Stem Cells (MSCs), including Dental Pulp Stem Cells (DPSCs), Stem Cells from Human Exfoliated Deciduous teeth (SHED), and Stem Cells From Apical Papilla (SCAP), have been extensively studied using highly sophisticatedin vitroandin vivosystems, yielding substantially improved understanding of their intriguing biological properties. Their capacity to reconstitute various dental and nondental tissues and the inherent angiogenic, neurogenic, and immunomodulatory properties of their secretome have been a subject of meticulous and costly research by various groups over the past decade. Key milestone achievements have exemplified their clinical utility in Regenerative Dentistry, as surrogate therapeutic modules for conventional biomaterial-based approaches, offering regeneration of damaged oral tissues instead of simply “filling the gaps.” Thus, the essential next step to validate these immense advances is the implementation of well-designed clinical trials paving the way for exploiting these fascinating research achievements for patient well-being: the ultimate aim of this ground breaking technology. This review paper presents a concise overview of the major biological properties of the human dental MSCs, critical for the translational pathway “from bench to clinic.”

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