scholarly journals In vitro comparison of ultrasonic shear versus ligating clip application in closure of cystic duct in cholecystectomy

2019 ◽  
Vol 6 (6) ◽  
pp. 2173
Author(s):  
Pritesh Yadav ◽  
Dharmendra Shah ◽  
Akshay Sutaria ◽  
Vishal Bhabhor
Keyword(s):  
Cystic Duct ◽  
Randomized Study ◽  
Postoperative Outcome ◽  
Bursting Pressure ◽  
Safe Alternative ◽  
Pneumatic Tourniquet ◽  
The Mean ◽  
Ultrasonic Shear ◽  
In Vitro Comparison

Background: Cholecystectomy is one of the most commonly performed surgery overall. One of the most important steps in the operation of cholecystectomy whether open or laparoscopic, is the closure of cystic duct which if not done properly can lead to disastrous postoperative outcome. This study was conducted with an aim to do in vitro comparison of ultrasonic shear and standard titanium ligating clips in closure of cystic duct with respect to their ability to withstand the pressure generated inside the gall bladder by pneumatic tourniquet device.Methods: A total 148 patients underwent open or laparoscopic cholecystectomy Between August 2016 to October 2017 at Sir Sayajirao Gaekwad Hospital, Baroda, were eligible for this prospective randomized study. A total of 16 cases were excluded from the study and specimens from 132 patients were included in the study. The eligible patients were randomized into two groups using sealed envelopes. The two groups were as follows: A) ligating titanium clips, and B) ultrasonic shear. Independent assessors were assigned to obtain cystic duct bursting pressure.Results: A comparison of the mean cystic duct bursting pressure between both the groups indicated the superiority of ultrasonic shear group over ligating titanium clip group.Conclusions: The mean cystic duct bursting pressure was higher in the ultrasonic shears group than in ligating clips group, higher than the maximum cystic duct bursting pressure (195 mmHg). The ultrasonic shear provides safe alternative to standard titanium ligating clip for cystic duct closure.

scholarly journals Is suture ligation of cystic duct in laparoscopic cholecystectomy a safe alternative to clipping? Our experience in a rural centre

2019 ◽  
Vol 6 (10) ◽  
pp. 3715
Author(s):  
K. R. Manoj Prabu ◽  
Dhinesh Balaji ◽  
Vishwanath M. Pai
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Postoperative Complications ◽  
Cystic Duct ◽  
Operating Time ◽  
Cost Effective ◽  
Safe Alternative ◽  
Suture Ligation ◽  
Medical College ◽  
The Mean ◽  
Mean Time

Background: Laparoscopic cholecystectomy is one of the most common surgeries performed nowadays. It remains an enigma regarding efficacy, safety and postoperative complications for using suture ligation for ligating cystic duct in laparoscopic cholecystectomy. The aim of the present study was to study the efficacy of ligating the cystic duct with sutures in laparoscopic cholecystectomy.Methods: This prospective study was performed between June 2018 and April 2019 in Saveetha Medical College and Hospital, in a rural center, Kanchipuram, India. All the patients included consented for the study. Patients who underwent subtotal cholecystectomy were excluded from the study.Results: The study included 70 patients who underwent laparoscopic cholecystectomy in a single unit. All cases were operated by a single surgeon. Of the 70 patients, the Cystic duct (CD) was simply ligated in 55 patients with CD <5 mm in diameter. The CD in 15 of those patients had to be divided and sutured in continuity for wide CD (>5 mm). The mean time for ligation of cystic duct was 5 min. Similarly, the mean time for ligation of cystic artery was 1.50 min. The mean operative time was 50 mins. There were no postoperative complications, such as bile leakage.Conclusions: SL of the CD is a very safe and secure alternative to the application of metal clips. It can be used in dilated CD, readily available and very cost effective and the complications of clips are avoided. The only disadvantage is that it needs expertise to perform and subsequently increasing the operating time. This technique is recommended in all laparoscopic cholecystectomies, especially in difficult cases.

Comparison of in vitro Fertilization/Intracytoplasmic Sperm Injection Outcomes in Patients receiving Recombinant Human Luteinizing Hormone vs Human Menopausal Gonadotropin Supplementation

2016 ◽  
Vol 7 (3) ◽  
pp. 77-81
Author(s):  
DB Usha Rajinikanthan ◽  
Thankam Varma
Keyword(s):  
Luteinizing Hormone ◽  
Gnrh Agonist ◽  
Intracytoplasmic Sperm Injection ◽  
Randomized Study ◽  
P Value ◽  
Human Menopausal Gonadotropin ◽  
Recombinant Fsh ◽  
The Mean ◽  
Lh Rh

ABSTRACT Objectives To compare the outcome of recombinant human luteinizing hormone (rh-LH) and human menopausal gonadotropin (hMG) supplementation in women undergoing in vitro fertilization/ intracytoplasmic sperm injection (IVF/ICSI) with recombinant follicle stimulating hormone (FSH) in the long gonadotropinreleasing hormone (GnRH) agonist stimulation protocol. Materials and methods It was a retrospective analysis of the case records of 90 consecutive women who underwent nondonor IVF/ICSI cycle with long GnRH agonist. All women received recombinant FSH on day 2/3 of the programming cycle. When the level of LH was < 0.5 mIU/mL during any phase of stimulation, then addition of LH either as rh-LH or hMG is given along with recombinant FSH. Results The number of oocytes collected, the number of oocytes in metaphase II (MII), and fertilization rate were similar in both groups. In addition, the mean number of embryos produced per cycle and the mean number of frozen embryos per cycle were similar in both groups. The cost of gonadotropin is similar in both groups. The ongoing pregnancy rate at 12 weeks was 20.4% after rh-FSH + hMG and 29.2% after rh-FSH + rh-LH (p-value = 0.092). Conclusion Supplementing recombinant FSH with recombinant LH (rh-LH) when compared with hMG does not show statistically significant increase in pregnancy rates. However, this study was a pilot venture to introduce the rh-LH into our practice and further randomized study is required to substantiate its use in assistive reproductive technology. How to cite this article Usha Rajinikanthan DB, Balasubramanyam S, Varma T. Comparison of in vitro Fertilization/Intracytoplasmic Sperm Injection Outcomes in Patients receiving Recombinant Luteinizing Hormone vs Human Menopausal Gonadotropin Supplementation. Int J Infertil Fetal Med 2016; 7(3):77-81.

In vitro comparison of the Groningen high resistance, Groningen low resistance and Provox speaking valves

1994 ◽  
Vol 108 (4) ◽  
pp. 321-324 ◽  
Author(s):  
Judith M. Heaton ◽  
Andrew J. Parker
Keyword(s):  
High Resistance ◽  
Voice Quality ◽  
Physical Parameters ◽  
Patient Acceptability ◽  
Pressure Flow ◽  
Low Resistance ◽  
Flow Profiles ◽  
The Mean ◽  
In Vitro Comparison

AbstractThis paper compares the physical parameters of the newer Groningen low resistance and Provox indwelling laryngectomy prostheses with the established and original Groningen device.In vitro pressure/flow profiles were determined, using specially designed apparatus, in 44 standard Groningen high resistance (GHR), 37 Groningen low resistance (GLR) and 19 Provox tracheo-oesophageal prostheses prior to insertion. GHR valves had significantly higher forward opening pressures than both the newer valves and the GHR was significantly higher than the Provox (p<0.01: Mann-Whitney U-test). The mean forward resistance of GHR was significantly higher than that of both; the Provox valve was significantly lower than that of GLR (p<0.0001: Mann-Whitney U-test). This may be of relevance with respect to patient acceptability, voice quality and effective duration of valve action.

scholarly journals IN VITRO COMPARISON OF THE ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF AZADIRACHTA INDICA LEAVES WITH GENTAMYCIN, AMPICILLIN, NITROFURANTOIN, AND COTRIMOXAZOLE ON BACTERIAL PATHOGENS ISOLATED FROM URINARY TRACT INFECTION PATIENTS

2017 ◽  
Vol 10 (8) ◽  
pp. 72 ◽  
Author(s):  
Meenakshi Jindal ◽  
Chauhan S
Keyword(s):  
Antibacterial Activity ◽  
Azadirachta Indica ◽  
Ethanolic Extract ◽  
Zone Of Inhibition ◽  
E Coli ◽  
Neem Leaves ◽  
Mean Diameter ◽  
The Mean ◽  
In Vitro Comparison

  Objective: In vitro comparison of the antibacterial activity of ethanolic extract of Azadirachta indica (neem) leaves with gentamycin, ampicillin, nitrofurantoin, and cotrimoxazole in bacterial pathogens isolated from urinary tract infection (UTI) patients.Methods: Ethanolic extract of neem leaves was prepared by the standard method. The antimicrobial activity against bacteria isolated from UTI patients was determined by agar well diffusion method and then mean zone of inhibition of neem extract was compared with a mean zone of inhibition of gentamycin, ampicillin, nitrofurantoin, and cotrimoxazole.Results: Among 200 samples which were included in the study, bacteria isolated were Escherichia coli (60%), Klebsiella pneumoniae (15%), Pseudomonas aeruginosa (11%), Enterococcus faecalis (5%), Proteus mirabilis (3%), and Staphylococcus aureus (6%). The result obtained was statistically analyzed by unpaired t-test. The difference in the mean diameter of the zone of inhibition between ethanolic extract of Azadirachta indica (neem) leaves and nitrofurantoin was statistically highly significant for K. pneumoniae (p<0.0001) and P. mirabilis (p=0.01) and insignificant for other bacteria. On comparing, the mean diameter of the zone of inhibition of ethanolic extract of A. indica (neem) leaves with ampicillin and cotrimoxazole, it was found to be statistically highly significant for all bacteria E. coli (p<0.0001), K. pneumoniae (p<0.0001), P. aeruginosa (p<0.0001) E. faecalis (p<0.001), and S. aureus (p<0.0001) expect P. mirabilis. The mean diameter of the zone of inhibition of ethanolic extract of A. indica (neem) leaves when compared with gentamycin was statistically highly significant for all the bacteria.Conclusion: The ethanolic extract of A. indica leaves has antibacterial activity. Antibacterial activity of ethanolic extract of A. indica leaves is comparable to nitrofurantoin for bacteria E. coli, P. aeruginosa, E. faecalis, and S. aureus. Ethanolic extract of A. indica leaves has similar antibacterial activity as ampicillin and cotrimoxazole for bacteria P. mirabilis. The antibacterial activity of gentamycin is less as compared to an ethanolic extract of A. indica leaves for all the bacteria.

Influence of Platelet Membrane Sialic Acid and Platelet-Associated IgG on Ageing and Sequestration of Blood Platelets in Baboons

1993 ◽  
Vol 70 (04) ◽  
pp. 676-680 ◽  
Author(s):  
H F Kotzé ◽  
V van Wyk ◽  
P N Badenhorst ◽  
A du P Heyns ◽  
J P Roodt ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Life Span ◽  
Blood Platelets ◽  
Senescent Cell ◽  
Platelet Membrane ◽  
Antigen Complex ◽  
The Mean ◽  
Aggregation Of Platelets

SummaryPlatelets were isolated from blood of baboons and treated with neuraminidase to remove platelet membrane sialic acid, a process which artificially ages the platelets. The platelets were then labelled with 111In and their mean life span, in vivo distribution and sites of Sequestration were measured. The effect of removal of sialic acid on the attachment of immunoglobulin to platelets were investigated and related to the Sequestration of the platelets by the spleen, liver, and bone marrow. Removal of sialic acid by neuraminidase did not affect the aggregation of platelets by agonists in vitro, nor their sites of Sequestration. The removal of 0.51 (median, range 0.01 to 2.10) nmol sialic acid/108 platelets shortened their life span by 75 h (median, range 0 to 132) h (n = 19, p <0.001), and there was an exponential correlation between the shortening of the mean platelet life span and the amount of sialic acid removed. The increase in platelet-associated IgG was 0.112 (median, range 0.007 to 0.309) fg/platelet (n = 25, p <0.001) after 0.79 (median, range 0.00 to 6.70) nmol sialic acid/108 platelets was removed (p <0.001). There was an exponential correlation between the shortening of mean platelet life span after the removal of sialic acid and the increase in platelet-associated IgG. The results suggest that platelet membrane sialic acid influences ageing of circulating platelets, and that the loss of sialic acid may have exposed a senescent cell antigen that binds IgG on the platelet membrane. The antibody-antigen complex may then provide a signal to the macrophages that the platelet is old, and can be phagocytosed and destroyed.

Efficacy and Safety of Oral Ivermectin and Topical Permethrin in the Treatment of Scabies

2020 ◽  
Vol 33 (1) ◽  
pp. 41-47
Author(s):  
Mohsena Akhter ◽  
Ishrat Bhuiyan ◽  
Zulfiqer Hossain Khan ◽  
Mahfuza Akhter ◽  
Gulam Kazem Ali Ahmad ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Close Contact ◽  
Randomized Study ◽  
Sarcoptes Scabiei ◽  
Efficacy And Safety ◽  
Common Skin ◽  
Group A ◽  
The Mean ◽  
The Difference ◽  
Group B

Background: Scabies is one of the most common skin diseases in our country. It is caused by the mite Sarcoptes scabiei var hominis, which is an ecto-parasite infesting the epidermis. Scabies is highly contagious. Prevalence is high in congested or densely populated areas. Individuals with close contact with an affected person should be treated with scabicidal which is available in both oral and topical formulations. The only oral but highly effective scabicidal known to date is Ivermectin. Amongst topical preparations, Permethrin 5 % cream is the treatment of choice. Objective: To evaluate the efficacy & safety of oral Ivermectin compared to topical Permethrin in the treatment of scabies. Methodology: This prospective, non-randomized study was conducted at the out-patient department of Dermatology and Venereology of Shaheed Suhrawardy Medical College & Hospital over a period of 6 months, from August 2016 to January 2017. The study population consisted of one hundred patients having scabies, enrolled according to inclusion criteria. They were divided into two groups. group A was subjected to oral Ivermectin and the group B to Permethrin 5% cream. Patients were followed up on day 7 and 14 for assessment of efficacy and safety. Result: The mean scoring with SD in group A (Ivermectin) and group B (Permethrin) were 8.26 ± 2.22 and 7.59 ± 2.01 respectively at the time of observation. The difference between the mean score of the two group is not significant (p=0.117) the mean scoring with SD in group A and group B were 4.54 ± 2.05 and 1.64 ± 1.84 respectively at 7thdays. The difference between the mean score of the two group is significant (p<0.001). The mean scoring with SD in group A and group B were 2.68± 2.35 and .36± 1.10 respectively at 14th day difference between the mean score of the group is significant (p<0.001). Conclusion: Topical application of permethrin 5% cream is more effective and safer than oral Ivermectin in the treatment of scabies. TAJ 2020; 33(1): 41-47

scholarly journals An in vitro comparison of tibial tray cementation using gun pressurization or pulsed lavage

2014 ◽  
Vol 38 (5) ◽  
pp. 967-971 ◽  
Author(s):  
Ulf J. Schlegel ◽  
Klaus Püschel ◽  
Michael M. Morlock ◽  
Katrin Nagel
Keyword(s):  
Tibial Tray ◽  
Pulsed Lavage ◽  
In Vitro Comparison

The Safety and Antimicrobial Properties of Stabilized Hypochlorous Acid in Acetic Acid Buffer for the Treatment of Acute Wounds—a Human Pilot Study and In Vitro Data

2021 ◽  
pp. 153473462110156
Author(s):  
Ewa A. Burian ◽  
Lubna Sabah ◽  
Klaus Kirketerp-Møller ◽  
Elin Ibstedt ◽  
Magnus M. Fazli ◽  
...  
Keyword(s):  
Pilot Study ◽  
Hypochlorous Acid ◽  
Treatment Time ◽  
Donor Site ◽  
Antimicrobial Properties ◽  
Prolonged Treatment ◽  
Wound Irrigation ◽  
The Mean ◽  
And Performance

Acute wounds may require cleansing to reduce the risk of infection. Stabilized hypochlorous acid in acetic buffer (HOCl + buffer) is a novel wound irrigation solution with antimicrobial properties. We performed a first-in-man, prospective, open-label pilot study to document preliminary safety and performance in the treatment of acute wounds. The study enrolled 12 subjects scheduled for a split-skin graft transplantation, where the donor site was used as a model of an acute wound. The treatment time was 75 s, given on 6 occasions. A total of 7 adverse events were regarded as related to the treatment; all registered as pain during the procedure for 2 subjects. One subject had a wound infection at the donor site. The mean colony-forming unit (CFU) decreased by 41% after the treatment, and the mean epithelialization was 96% on both days 14 (standard deviation [SD] 8%) and 21 (SD 10%). The study provides preliminary support for the safety, well-tolerance, and efficacy of HOCl + buffer for acute wounds. The pain was frequent although resolved quickly. Excellent wound healing and satisfying antimicrobial properties were observed. A subsequent in vitro biofilm study also indicated good antimicrobial activity against Pseudomonas aeruginosa with a 96% mean reduction of CFU, when used for a treatment duration of 15 min ( P < .0001), and a 50% decrease for Staphylococcus aureus ( P = .1010). Future larger studies are needed to evaluate the safety and performance of HOCl + buffer in acute wounds, including the promising antimicrobial effect by prolonged treatment on bacterial biofilms.

scholarly journals Formulation and in vitro evaluation of self-nanoemulsifying liquisolid tablets of furosemide

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lena Dalal ◽  
Abdul Wahab Allaf ◽  
Hind El-Zein
Keyword(s):  
Theoretical Model ◽  
Castor Oil ◽  
In Vivo Studies ◽  
Coating Materials ◽  
Peg 400 ◽  
The Mean ◽  
Usp Apparatus ◽  
Solid System

AbstractSelf-nanoemulsifying drug delivery systems (SNEDDS) were used to enhance the dissolution rate of furosemide as a model for class IV drugs and the system was solidified into liquisolid tablets. SNEDDS of furosemide contained 10% Castor oil, 60% Cremophor EL, and 30% PEG 400. The mean droplets size was 17.9 ± 4.5 nm. The theoretical model was used to calculate the amounts of the carrier (Avicel PH101) and coating materials (Aerosil 200) to prepare liquisolid powder. Carrier/coating materials ratio of 5/1 was used and Ludipress was added to the solid system, thus tablets with hardness of 45 ± 2 N were obtained. Liquisolid tablets showed 2-folds increase in drug release as compared to the generic tablets after 60 min in HCl 0.1 N using USP apparatus-II. Furosemide loaded SNEDDS tablets have great prospects for further in vivo studies, and the theoretical model is useful for calculating the adequate amounts of adsorbents required to solidify these systems.

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