scholarly journals Novel Albumin Gel-platelet-rich fibrin mixture (Alb-PRF); where do we stand?

2022 ◽  
Vol 8 (4) ◽  
pp. 239-241
Author(s):  
Vinayaka Ambujakshi Manjunatha ◽  
Trinath Kishore Damera ◽  
Akshay Kumar T K ◽  
Rupinder Jyot Singh ◽  
Tanmay Popat ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Translational Research ◽  
Blood Cells ◽  
Autologous Blood ◽  
Periodontal Surgery ◽  
Platelet Rich Fibrin ◽  
New Material ◽  
Two Stages

AlbPRF is a blood by-product, without additives, using only autologous blood, which after centrifugation will be produced in two stages: heating and incorporation: heating of the serum and low platelet plasma and incorporation of cells (GF and PRF cytokines liquid, removed from the junction of the leukocyte zone and the red blood cells). This new biomaterial has already been tested in vitro and translational research with this new material has already started. Excellent results can be expected from the use of AlbPRF, not only for oral/periodontal surgery applications but also for use in facial medicine and aesthetics.

scholarly journals ОСОБЛИВОСТІ ТРАНСПОРТУ ЦИНКУ І КАДМІЮ ЧЕРЕЗ МЕМБРАНИ ЕРИТРОЦИТІВ ЗА ДІЇ ПІДВИЩЕНИХ КОНЦЕНТРАЦІЙ ЇХ ІОНІВ У ВОДІ

2021 ◽  
Vol 81 (1-2) ◽  
pp. 31-38
Author(s):  
V. O. Khomnechuk ◽  
Yu. I. Senyk ◽  
V. Z. Kurant
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Biological Membrane ◽  
Membrane Surface ◽  
Membrane Integrity ◽  
Erythrocyte Membranes ◽  
Cell Membrane Integrity ◽  
Cadmium Transport ◽  
Two Stages

The peculiarities of Zinc and Cadmium transport through carp erythrocyte membranes in vitro after 14 acclimatization of fish to elevated concentrations of Zn2+ and Cd2+ ions were studied. It has been established, that the uptake of Zinc and Cadmium by carp erythrocytes is to a certain extent a regulated and concentration-dependent process. The uptake of Zinc and Cadmium has been shown to involve two stages: rapid sorption of metals by the membrane surface of fish erythrocytes and slower penetration through the apical membrane of red blood cells. The increase in the value of the Michaelis constant of sorption of metal ions by erythrocytes of fish acclimatized to elevated concentrations of Zn2+ and Cd2+ ions indicates a decrease in their affinity for metals, which is the adaptive response of cells to elevated concentrations of toxicants. The nature of the penetration of Zinc and Cadmium through the biological membrane of red blood cells of fish acclimatized to the action of 2 MPC of Zn2+ and Cd2+ ions is obviously the result of significant violations of cell membrane integrity, and, consequently, reduced ability to regulate and limit the flow of studied metals .

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

Effect of liposomal curcumin on red blood cells in vitro

2013 ◽  
Vol 1 (Suppl. 1) ◽  
pp. A4.1
Author(s):  
Angela Storka
Keyword(s):  
Red Blood Cells ◽  
Blood Cells

scholarly journals THE SYNTHESIS OF PROTOPORPHYRIN IN VITRO BY RED BLOOD CELLS OF THE DUCK

1950 ◽  
Vol 183 (2) ◽  
pp. 757-765 ◽  
Author(s):  
David Shemin ◽  
Irving M. London ◽  
D. Rittenberg
Keyword(s):  
Red Blood Cells ◽  
Blood Cells

Nucleated red blood cells participate in myocardial regeneration in the toad Bufo Gargarizan Gargarizan

2021 ◽  
pp. 153537022110132
Author(s):  
Shu-Qin Liu ◽  
Xiao-Ye Hou ◽  
Feng Zhao ◽  
Xiao-Ge Zhao
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cardiac Injury ◽  
Myocardial Regeneration ◽  
Matrix Metalloproteinase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cardiac Tissues ◽  
Nucleated Red Blood Cells ◽  
Model Animal

Heart regeneration is negligible in humans and mammals but remarkable in some ectotherms. Humans and mammals lack nucleated red blood cells (NRBCs), while ectotherms have sufficient NRBCs. This study used Bufo gargarizan gargarizan, a Chinese toad subspecies, as a model animal to verify our hypothesis that NRBCs participate in myocardial regeneration. NRBC infiltration into myocardium was seen in the healthy toad hearts. Heart needle-injury was used as an enlarged model of physiological cardiomyocyte loss. It recovered quickly and scarlessly. NRBC infiltration increased during the recovery. Transwell assay was done to in vitro explore effects of myocardial injury on NRBCs. In the transwell system, NRBCs could infiltrate into cardiac pieces and could transdifferentiate toward cardiomyocytes. Heart apex cautery caused approximately 5% of the ventricle to be injured to varying degrees. In the mildly to moderately injured regions, NRBC infiltration increased and myocardial regeneration started soon after the inflammatory response; the severely damaged region underwent inflammation, scarring, and vascularity before NRBC infiltration and myocardial regeneration, and recovered scarlessly in four months. NRBCs were seen in the newly formed myocardium. Enzyme-linked immunosorbent assay and Western blotting showed that the levels of tumor necrosis factor-α, interleukin- 1β, 6, and11, cardiotrophin-1, vascular endothelial growth factor, erythropoietin, matrix metalloproteinase- 2 and 9 in the serum and/or cardiac tissues fluctuated in different patterns during the cardiac injury-regeneration. Cardiotrophin-1 could induce toad NRBC transdifferentiation toward cardiomyocytes in vitro. Taken together, the results suggest that the NRBC is a cell source for cardiomyocyte renewal/regeneration in the toad; cardiomyocyte loss triggers a series of biological processes, facilitating NRBC infiltration and transition to cardiomyocytes. This finding may guide a new direction for improving human myocardial regeneration.

scholarly journals Multimodal Diagnostics of Microrheologic Alterations in Blood of Coronary Heart Disease and Diabetic Patients

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 76
Author(s):  
Anastasia Maslianitsyna ◽  
Petr Ermolinskiy ◽  
Andrei Lugovtsov ◽  
Alexandra Pigurenko ◽  
Maria Sasonko ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Optical Methods ◽  
Diabetic Patients ◽  
Aggregation Index ◽  
Significant Difference

Coronary heart disease (CHD) has serious implications for human health and needs to be diagnosed as early as possible. In this article in vivo and in vitro optical methods are used to study blood properties related to the aggregation of red blood cells in patients with CHD and comorbidities such as type 2 diabetes mellitus (T2DM). The results show not only a significant difference of the aggregation in patients compared to healthy people, but also a correspondence between in vivo and in vitro parameters. Red blood cells aggregate in CHD patients faster and more numerously; in particular the aggregation index increases by 20 ± 7%. The presence of T2DM also significantly elevates aggregation in CHD patients. This work demonstrates multimodal diagnostics and monitoring of patients with socially significant pathologies.

In Vitro Assay for Single-cell Characterization of Impaired Deformability in Red Blood Cells under Recurrent Episodes of Hypoxia

Lab on a Chip ◽  
2021 ◽  
Author(s):  
YUHAO QIANG ◽  
Jia Liu ◽  
Ming Dao ◽  
E Du
Keyword(s):  
Shear Stress ◽  
Red Blood Cells ◽  
Single Cell ◽  
Blood Cells ◽  
Blood Circulation ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Cyclic Shear

Red blood cells (RBCs) are subjected to recurrent changes in shear stress and oxygen tension during blood circulation. The cyclic shear stress has been identified as an important factor that...

The Plasma Fraction of Stored Packed Red Blood Cells Classically Activates Monocytes in Vitro

2014 ◽  
Vol 186 (2) ◽  
pp. 683
Author(s):  
J.A. Yi ◽  
K. Lo ◽  
C.C. Silliman ◽  
B.H. Edil ◽  
R.D. Schulick ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Packed Red Blood Cells ◽  
Plasma Fraction
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