Bacteriological profile and antibiotic sensitivity pattern in various body fluids –A retrospective study

Introduction : Neonatal sepsis remains an important cause of neonatal morbidity and mortality in NICU setup and a major challenge for the neonatologists. The prevalent organisms and their antibiotic resistance patterns evolve with time and with the usage of antimicrobials.Aims and Objectives : To analyze the bacteriological profile and antibiotic resistance patterns of proven neonatal sepsis cases in Dhaka Shishu Hospital.Materials and Methods : The study was Prospective observational study. All the clinically suspected cases of Neonatal Sepsis admitted to the Neonatal word from January 2015 to December 2015 were included in the study. Clinically suspected cases of neonatal sepsis further evaluated with blood cultures and antibiotic susceptibility testing using the Kirby Bauer disc diffusion method. Data was collected for the following variables: Demographic profile, haematological profile, blood culture result and antibiotic sensitivity patterns.Results : Total 96 cases clinically suspected neonatal sepsis were included in the study. Among them 29 cases (30.21%) was culture positive septicemia. Klebsiella pneumoniae was the most common isolate accounting for 31.03% cases followed by Escherichia coli 27.59%. In Klebsiella infection most common sensitivity were Imipenum and Ciprofloxacin which were 77.78 and 33.33% respectively. In E.coli common sensitive drugs were Imipenum and Amikacin. In serratia common sensitive drugs were Imipenum, Netlmicin and Ciprofloxacin.Conclusion : K. pneumoniae was the most common pathogen. Most common sensitivity was Imipenum, Amikacin, Ciprofloxacin, Gentamicin, Netlmicin, and Ceftazidime,.Northern International Medical College Journal Vol.8(1) July 2016: 174-177

Download Full-text

A retrospective study on antibiotic sensitivity pattern of pathogens isolated from blood culture in cases of late-onset neonatal sepsis at a neonatal intensive care unit

National Journal of Physiology Pharmacy and Pharmacology ◽

10.5455/njppp.2018.8.1145016122017 ◽

2018 ◽

pp. 1

Author(s):

Shruthi S ◽

Narendranath Sanji ◽

Chaitali Raghoji ◽

Guruprasad G ◽

Shashikala H

Keyword(s):

Intensive Care Unit ◽

Intensive Care ◽

Retrospective Study ◽

Blood Culture ◽

Neonatal Intensive Care Unit ◽

Neonatal Sepsis ◽

Neonatal Intensive Care ◽

Late Onset ◽

Antibiotic Sensitivity ◽

Sensitivity Pattern

Download Full-text

Incidence, bacteriological profile and antibiotic sensitivity pattern of neonatal sepsis in a tertiary health facility in Abuja, North-central Nigeria.

10.21203/rs.2.21673/v2 ◽

2021 ◽

Author(s):

Olutunde Oluyinka ◽

Kareem I. Airede ◽

Kudi E. Olateju ◽

Obaro K. Stephen ◽

Nosakhare Izevbigie ◽

...

Keyword(s):

Blood Culture ◽

Incidence Rate ◽

Neonatal Sepsis ◽

White Cell Count ◽

Antibiotic Sensitivity ◽

Signs And Symptoms ◽

Special Care Unit ◽

Bacteriological Profile ◽

Sensitivity Pattern ◽

Pseudomonas Luteola

Abstract Background: Neonatal sepsis is commonly caused by bacteria in the first 28 days of life. Due to diagnostic limitations in developing settings, prompt laboratory identification of causative organisms is usually a challenge. To prevent mortality, clear knowledge of bacteria and their antibiotic sensitivity patterns are important for prompt empirical treatment. Methods: This prospective study enrolled 339 newborns with signs and symptoms suggestive of neonatal sepsis out of 645 that were admitted into the special care unit of the University of Teaching Hospital during the study period. Socio-demographic and clinical profiles of the newborns were obtained using a questionnaire and blood culture was done from every enrolled newborn (339 newborns) using BACTEC 9050. The bacteriological profile and antibiotic sensitivity pattern of newborns with confirmed neonatal sepsis were documented. Results: A total of 339 newborn were admitted for probable sepsis out of a total admission of 645 newborns during the study period based on clinical features and initial laboratory work-up. Forty-six of the 645 newborns (46/645) had culture proven sepsis resulting in a neonatal sepsis incidence rate of 71.3 (95%CI 50.7-91.9) per 1000 admitted newborns. Seventeen of the 46 confirmed sepsis cases were among the 1322 newborns delivered within the study facility during the study period giving an in-hospital neonatal sepsis incidence rate of 12.9 (95% CI 6.7-19.0) per 1000 live births. Amongst the 46 babies with positive blood culture, 27/46 (58.7%) had normal white cell count while the remaining 19/46 (41.3%) had abnormal results. Fifty-two (52) counts of bacteria categorized into 11 bacteria species were isolated from the 46 positive blood cultures. Enterococcus spp and streptococcus species were the commonest gram-positive while Escherichia coli and Pseudomonas luteola were the commonest gram-negative bacteria isolates. Imipenem, amoxicillin/clavulanic acid, Vancomycin, and ofloxacin had the widest coverage of bacteria isolated from newborn with sepsis. Conclusion: Neonatal sepsis is still prevalent in our environment and compared to previous documented isolates and sensitivity pattern, the bacteria causes, and their antibiotic sensitivity patterns appears to be changing.

Download Full-text

Clinical and bacteriological profile of neonatal sepsis with emerging resistance patterns

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20184281 ◽

2018 ◽

Vol 5 (6) ◽

pp. 2203 ◽

Cited By ~ 1

Author(s):

Rohitashwa Rajana ◽

Dhan Raj Bagri ◽

J. N. Sharma ◽

Vijay Agrawal

Keyword(s):

Birth Weight ◽

Low Birth Weight ◽

Neonatal Sepsis ◽

Antibiotic Sensitivity ◽

Polymyxin B ◽

Laboratory Evaluation ◽

Gram Positive ◽

Gram Negative ◽

Bacteriological Profile ◽

Sensitivity Pattern

Background: The present study was designed to evaluate the clinical spectrum, bacteriological profile, antibiotic sensitivity pattern and mortality due to neonatal septicemia in neonates admitted in neonatal units attached to the SMS Medical College, Jaipur.Methods: In born and out born babies of postnatal age up to 28 days who were bacteriological proven cases of septicemia were subjected to history, clinical examination and laboratory evaluation and data were analyzed statistically.Results: Out of 150 cases 67.33% neonates were preterm and 77.33% were low birth weight neonates. Gram negative organisms were most common cause of septicemia (66.6%), Both the Gram negative and Gram positive organisms were sensitive to piperacillin-tazobactam, linezolid, fosfomycin, teicoplanin, polymyxin B, colistin, ofloxacin. Both the Gram negative and Gram positive organisms were resistant against cephalosporins, ampicillin, Amoxyclav, cotrimoxazole. Gram negative isolates were most sensitive to Polymyxin B (70%) and had the highest resistance to cefepime (36%). Gram-positive organisms were most sensitive to vancomycin (84%) and linezolid (82%). Highest resistance was noted from Amoxyclav (52%).Conclusions: Preterm (<37 week) and low birth weight (<2500gm) neonates are considered as major susceptible causes of neonatal sepsis. Proper hygiene and hand washing, early detection of sepsis and judicial use of antibiotics to prevent multidrug resistance is needful in our setup.

Download Full-text