Definition of kinetic characteristics of biochemical oxidation of substances of sewage butter-fat industries

The purpose of research is heifers protein dairy raising of Holsteined Black-Motley cattle of Samara type. Studies on the identification of polymorphism and the definition of occurrence frequencies of alleles and genotypes at loci kappa-casein gene (CSN3), beta-lactoglobulin (LGB), and complex genotypes (LGB/CSN3) Holsteined Black-Motley breed cows of Samara type. It was established the influence of the genotypes of these genes for heifers dairy production. The cattle of CJSC «Lunacharsky» with genotype CSN3ВВ for dairy production during 305 days of lactation had an advantage over CSN3АА genotype at 348 kg and 468 kg in the CSN3АВ and butter fat content showed significant superiority to 0.16% (P˂0.001) and 0.23% (Р˂0,01). At the exit of butter fat and 28.1 to 23.9 kg of milk protein by 15.3 and 12.7 kg, respectively. The heifers of «Breeding Farm “Druzhba”» with these genotypes are equal. The heifers with genotype LGBВВ CJSC «Lunacharsky» had lower level to genotypes LGBАА, LGBАВ and the level of dairy production at 208 kg and 75 kg of milk. But had the advantage of butter fat content 0.08%, protein by 0.05-0.03%. The heifers of «Breeding Farm “Druzhba”» with the heterozygous genotype LGBАВ genotype produced significantly more at 478 kg (P<0,05) and 150 kg of milk compared with homozygous genotypes LGBВВ, LGBАА and that increased butter fat output 19.8 kg (P<0,05) and protein – 14.5 kg (P<0,05).

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Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

Reproduction ◽

10.1530/rep-12-0125 ◽

2012 ◽

Vol 144 (3) ◽

pp. 339-347 ◽

Cited By ~ 9

Author(s):

Alberto Contri ◽

Daniele Zambelli ◽

Massimo Faustini ◽

Marco Cunto ◽

Alessia Gloria ◽

...

Keyword(s):

Neural Networks ◽

Artificial Neural Networks ◽

Sperm Motility ◽

Principal Component ◽

Domestic Cat ◽

Computer Assisted ◽

Multivariate Approach ◽

Kinetic Characteristics ◽

Artificial Neural ◽

Definition Of

This study was designed for the identification of different sperm kinetic subpopulations in feline semen using artificial neural networks (ANNs) and for the evaluation of the effect of ejaculation on motility patterns of these subpopulations. Seven tomcats presented for routine orchiectomy were electroejaculated, and after 5 days, orchiectomized and epididymal tail sperms were collected. Sperm motility characteristics were evaluated using a computer-assisted sperm analyzer that provided individual kinetic characteristics of each spermatozoon. A total of 23 400 spermatozoa for electroejaculated and 9200 for epididymal tail samples were evaluated using a multivariate approach, comprising principal component analysis and ANN classification. The multivariate approach allowed the identification and characterization of three different and well-defined sperm subpopulations. There were significant differences before (epididymal tail spermatozoa) and after (electroejaculated sperm) ejaculation in sperm kinetic subpopulation characteristics. In both epididymal and ejaculated samples, the majority of subpopulation was characterized by high velocity and progressiveness; however, the electroejaculated samples showed significantly higher values, suggesting that the microenvironment of the epididymal tail could affect the sperm motility or, alternatively, seminal plasma could increase the kinetic characteristics of the spermatozoa, indicating that only after ejaculation, the spermatozoa express their motility potential. Nevertheless, further studies are required to clarify the functional significance of each kinetic subpopulation.

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Kinetic characterization of dihydrofolate reductase from Drosophila melanogaster

Biochemistry and Cell Biology ◽

10.1139/o90-161 ◽

1990 ◽

Vol 68 (9) ◽

pp. 1075-1082 ◽

Cited By ~ 12

Author(s):

Susan L. Rancourt ◽

Virginia K. Walker

Keyword(s):

Drosophila Melanogaster ◽

Dihydrofolate Reductase ◽

Competitive Inhibitor ◽

Structural Features ◽

Acidic Ph ◽

Kinetic Characteristics ◽

Ph Values ◽

Definition Of ◽

Organic Mercurials

The kinetic characteristics of a purified insect dihydrofolate reductase (DHFR) have been described. The Km values for the substrate dihydrofolate and the cofactor NADPH have been estimated by primary and secondary Hanes plots to be 0.3 and 5.2 μM, respectively. Drosophila melanogaster DHFR can use folate and NADH at acidic pH values, but at a much lower rate than the preferred substrate and cofactor. Folic acid is a partial competitive inhibitor of Drosophila DHFR (Ki = 0.4 μM) and trimethoprim is a complete competitive inhibitor (Ki = 5.4 μM). Methotrexate binds less tightly to the Drosophila enzyme than to many other DHFRs (Kd = 0.9 nM). Drosophila DHFR is inhibited by KCl and organic mercurials and is slightly activated by urea. These data indicate that Drosophila DHFR has some characteristics which are typical of vertebrate DHFRs and others which are typical of prokaryotic DHFRs. The study of this enzyme, therefore, should aid in the definition of the structural features that are responsible for the kinetic characteristics in different DHFRs.Key words: dihydrofolate reductase, Drosophila melanogaster, methotrexate.

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Introductory paper

Symposium - International Astronomical Union ◽

10.1017/s0074180900053031 ◽

1966 ◽

Vol 24 ◽

pp. 3-5

Author(s):

W. W. Morgan

Keyword(s):

Line Intensity ◽

Classification Scheme ◽

Two Dimensional ◽

Spectral Classification ◽

Dimensional Array ◽

Rr Lyrae ◽

Metallic Line ◽

Introductory Paper ◽

Parameter Varying ◽

Definition Of

1. The definition of “normal” stars in spectral classification changes with time; at the time of the publication of theYerkes Spectral Atlasthe term “normal” was applied to stars whose spectra could be fitted smoothly into a two-dimensional array. Thus, at that time, weak-lined spectra (RR Lyrae and HD 140283) would have been considered peculiar. At the present time we would tend to classify such spectra as “normal”—in a more complicated classification scheme which would have a parameter varying with metallic-line intensity within a specific spectral subdivision.

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2.2. Working Group 2: Conceptual Definitions of Reference Coordinate Systems for Earth Dynamics

International Astronomical Union Colloquium ◽

10.1017/s0252921100050867 ◽

1975 ◽

Vol 26 ◽

pp. 21-26

Keyword(s):

Coordinate System ◽

Working Group ◽

General Character ◽

Coordinate Systems ◽

Reference Coordinate System ◽

Group 2 ◽

Definition Of ◽

Earth Dynamics

An ideal definition of a reference coordinate system should meet the following general requirements:1. It should be as conceptually simple as possible, so its philosophy is well understood by the users.2. It should imply as few physical assumptions as possible. Wherever they are necessary, such assumptions should be of a very general character and, in particular, they should not be dependent upon astronomical and geophysical detailed theories.3. It should suggest a materialization that is dynamically stable and is accessible to observations with the required accuracy.

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Symbiotic Stars at Optical, Infrared and Radio Wavelengths

International Astronomical Union Colloquium ◽

10.1017/s025292110007336x ◽

1979 ◽

Vol 46 ◽

pp. 125-149 ◽

Cited By ~ 3

Author(s):

David A. Allen

Keyword(s):

Temperature Regime ◽

Type Star ◽

Stellar Systems ◽

Symbiotic Stars ◽

Lower Excitation ◽

Definition Of ◽

Original Type

No paper of this nature should begin without a definition of symbiotic stars. It was Paul Merrill who, borrowing on his botanical background, coined the termsymbioticto describe apparently single stellar systems which combine the TiO absorption of M giants (temperature regime ≲ 3500 K) with He II emission (temperature regime ≳ 100,000 K). He and Milton Humason had in 1932 first drawn attention to three such stars: AX Per, CI Cyg and RW Hya. At the conclusion of the Mount Wilson Ha emission survey nearly a dozen had been identified, and Z And had become their type star. The numbers slowly grew, as much because the definition widened to include lower-excitation specimens as because new examples of the original type were found. In 1970 Wackerling listed 30; this was the last compendium of symbiotic stars published.

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Immunoferritin localization of intracellular antigens in positively stained frozen sections

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010008170x ◽

1978 ◽

Vol 36 (2) ◽

pp. 168-169

Author(s):

K. T. Tokuyasu

Keyword(s):

Surface Tension ◽

Water Surface ◽

Thin Layers ◽

The Other ◽

Positive Staining ◽

Frozen Sections ◽

The Past ◽

Ultrathin Frozen Sections ◽

Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

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Initial Polymerization Sites Indicated During Mitochondrial Oxidation of Diaminobenzidine after Toluene Treatment

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100079498 ◽

1977 ◽

Vol 35 ◽

pp. 408-409

Author(s):

W. A. Shannon ◽

M. A. Matlib

Keyword(s):

Membrane Permeability ◽

Cytochrome Oxidase ◽

Rat Liver ◽

Precise Definition ◽

Cytochemical Localization ◽

Oxidative Reaction ◽

Mitochondrial Oxidation ◽

Definition Of ◽

Exogenous Substrates

Numerous studies have dealt with the cytochemical localization of cytochrome oxidase via cytochrome c. More recent studies have dealt with indicating initial foci of this reaction by altering incubation pH (1) or postosmication procedure (2,3). The following study is an attempt to locate such foci by altering membrane permeability. It is thought that such alterations within the limits of maintaining morphological integrity of the membranes will ease the entry of exogenous substrates resulting in a much quicker oxidation and subsequently a more precise definition of the oxidative reaction.The diaminobenzidine (DAB) method of Seligman et al. (4) was used. Minced pieces of rat liver were incubated for 1 hr following toluene treatment (5,6). Experimental variations consisted of incubating fixed or unfixed tissues treated with toluene and unfixed tissues treated with toluene and subsequently fixed.

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Application of Scanning Electron Microscopy to Medical Research

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100061963 ◽

1969 ◽

Vol 27 ◽

pp. 12-13

Author(s):

J. D. Hutchison

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Medical Research ◽

Prosthetic Heart Valve ◽

School Of Medicine ◽

Transmission Electron ◽

Definition Of ◽

Mechanism Of Failure ◽

The Brain ◽

Scanning Electron

When the transmission electron microscope was commercially introduced a few years ago, it was heralded as one of the most significant aids to medical research of the century. It continues to occupy that niche; however, the scanning electron microscope is gaining rapidly in relative importance as it fills the gap between conventional optical microscopy and transmission electron microscopy.IBM Boulder is conducting three major programs in cooperation with the Colorado School of Medicine. These are the study of the mechanism of failure of the prosthetic heart valve, the study of the ultrastructure of lung tissue, and the definition of the function of the cilia of the ventricular ependyma of the brain.

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Selection and Preparation of Specific Tissue Regions For Tem Using Large Epoxy-Embedded Blocks

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007206x ◽

1973 ◽

Vol 31 ◽

pp. 324-325 ◽

Cited By ~ 1

Author(s):

P. M. Lowrie ◽

W. S. Tyler

Keyword(s):

Electron Microscopy ◽

Anatomical Structures ◽

Ultrastructural Studies ◽

Transmission Electron ◽

Microscopic Evaluation ◽

Embedded Blocks ◽

Specific Tissue ◽

Definition Of ◽

Areas Of Interest ◽

Selection Of

The importance of examining stained 1 to 2μ plastic sections by light microscopy has long been recognized, both for increased definition of many histologic features and for selection of specimen samples to be used in ultrastructural studies. Selection of specimens with specific orien ation relative to anatomical structures becomes of critical importance in ultrastructural investigations of organs such as the lung. The uantity of blocks necessary to locate special areas of interest by random sampling is large, however, and the method is lacking in precision. Several methods have been described for selection of specific areas for electron microscopy using light microscopic evaluation of paraffin, epoxy-infiltrated, or epoxy-embedded large blocks from which thick sections were cut. Selected areas from these thick sections were subsequently removed and re-embedded or attached to blank precasted blocks and resectioned for transmission electron microscopy (TEM).

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