Application of Scanning Electron Microscopy to Medical Research

When the transmission electron microscope was commercially introduced a few years ago, it was heralded as one of the most significant aids to medical research of the century. It continues to occupy that niche; however, the scanning electron microscope is gaining rapidly in relative importance as it fills the gap between conventional optical microscopy and transmission electron microscopy.IBM Boulder is conducting three major programs in cooperation with the Colorado School of Medicine. These are the study of the mechanism of failure of the prosthetic heart valve, the study of the ultrastructure of lung tissue, and the definition of the function of the cilia of the ventricular ependyma of the brain.

Copper Localization in Cirrhotic Rat Liver by Scanning Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100062099 ◽

1969 ◽

Vol 27 ◽

pp. 38-39 ◽

Cited By ~ 1

Author(s):

J. C. Russ ◽

E. McNatt

Keyword(s):

Electron Microscopy ◽

Endoplasmic Reticulum ◽

Electron Microscope ◽

Copper Acetate ◽

Lead Citrate ◽

Dense Bodies ◽

Transmission Electron ◽

Electron Mode ◽

In order to study the retention of copper in cirrhotic liver, rats were made cirrhotic by carbon tetrachloride inhalation twice weekly for three months and fed 0.2% copper acetate ad libidum in drinking water for one month. The liver tissue was fixed in osmium, sectioned approximately 2000 Å thick, and stained with lead citrate. The section was examined in a scanning electron microscope (JEOLCO JSM-2) in the transmission electron mode.Figure 1 shows a typical area that includes a red blood cell in a sinusoid, a disse, and a portion of the cytoplasm of a hepatocyte which contains several mitochondria, peribiliary dense bodies, glycogen granules, and endoplasmic reticulum.

Scanning Secondary Electron Microscopy of Myofibrils Using Transmission Techniques

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100116401 ◽

1975 ◽

Vol 33 ◽

pp. 556-557

Author(s):

M. K. Lamvik

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Carbon Films ◽

Photographic Recording ◽

Transmission Electron ◽

Thin Carbon ◽

The Common ◽

Scanning Electron ◽

Better Than ◽

Cellular Organelles

When observing small objects such as cellular organelles by scanning electron microscopy, it is often valuable to use the techniques of transmission electron microscopy. The common practice of mounting and coating for SEM may not always be necessary. These possibilities are illustrated using vertebrate skeletal muscle myofibrils.Micrographs for this study were made using a Hitachi HFS-2 scanning electron microscope, with photographic recording usually done at 60 seconds per frame. The instrument was operated at 25 kV, with a specimen chamber vacuum usually better than 10-7 torr. Myofibrils were obtained from rabbit back muscle using the method of Zak et al. To show the component filaments of this contractile organelle, the myofibrils were partially disrupted by agitation in a relaxing medium. A brief centrifugation was done to clear the solution of most of the undisrupted myofibrils before a drop was placed on the grid. Standard 3 mm transmission electron microscope grids covered with thin carbon films were used in this study.

Organoclay-reinforced polyethersulfone-modified epoxy-based hybrid nanocomposites

High Performance Polymers ◽

10.1177/0954008311418385 ◽

2011 ◽

Vol 23 (7) ◽

pp. 526-534 ◽

Cited By ~ 8

Author(s):

Yang Wang ◽

Boming Zhang ◽

Jinrui Ye

Keyword(s):

Electron Microscopy ◽

Fracture Toughness ◽

Electron Microscope ◽

Optical Microscope ◽

Mechanical Analysis ◽

Hybrid Nanocomposites ◽

X Ray Diffraction ◽

Transmission Electron ◽

Modified Epoxy ◽

Hybrid nanocomposites were successfully prepared by the incorporation of polyethersulfone (PES) and organoclay into epoxy resin. They had higher fracture toughness than the prepared PES/epoxy blend and organoclay/epoxy nanocomposites. The microstructures of the hybrid nanocomposites were studied. They were comprised of homogeneous PES/epoxy semi-interpenetrating network (semi-IPN) matrices and organoclay micro-agglomerates made up of tactoid-like regions composed of ordered exfoliated organoclay with various orientations. The former was confirmed with dynamic mechanical analysis, scanning electron microscopy and transmission electron microscopy, while the latter was successfully observed with X-ray diffraction measurements, optical microscope, scanning electron microscope and transmission electron microscope. The improvement of their fracture toughness was due to the synergistic toughening effect of the PES and the organoclay and related to their microstructures.

Preparation and Characterization of Monodisperse Ceria Microspheres

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.434-435.850 ◽

2010 ◽

Vol 434-435 ◽

pp. 850-852

Author(s):

Qi Wang ◽

Bo Yin ◽

Zhen Wang ◽

Gen Li Shen ◽

Yun Fa Chen

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Particle Size ◽

Electron Microscope ◽

Crystalline Form ◽

X Ray ◽

Transmission Electron ◽

Particle Size Analyzer ◽

In present work, ceria microspheres were synthesized by template hydrothermal method. Crystalline form of the as-synthesized ceria microspheres was defined by X-ray powder diffraction (XRD) and high resolution transmission electron microscopy (HRTEM). Dispersibility of ceria microspheres was comprehensively characterized using scanning electron microscope (SEM) observation and laser particle size analyzer. Furthermore, the ultraviolet light absorption performances of ceria microspheres with several different sizes were compared by ultraviolet visible spectrophotometer. The results showed that ceria microspheres presented excellent UV absorbent property and the size influence was remarkable.

Preparation of Helminths For Scanning Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072630 ◽

1973 ◽

Vol 31 ◽

pp. 438-439

Author(s):

Robert W. Weise

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Transmission Electron Microscope ◽

Room Temperature ◽

Parasitic Nematodes ◽

Live Animal ◽

Transmission Electron ◽

Critical Point Drying ◽

The role that scanning electron microscopy (SEM) is playing in descriptive helminthology is becoming more apparent in the literature. However, the majority of papers on the SEM of helminths have used conventional or modified light microscope techniques of fixation and dehydration, and not established SEM techniques in which freeze- and critical point-drying are routinely used. The present investigation was undertaken to examine the applicability of modified scanning and transmission electron microscope techniques for the preparation of certain helminths for SEM.Method I.– Live animal-parasitic nematodes were fixed in 6% phosphate buffered glutaraldehyde for 24 hr at room temperature.

ISTFA 2002: Conference Proceedings from the 28th International Symposium for Testing and Failure Analysis ◽

STEM (Scanning Transmission Electron Microscopy) in a SEM (Scanning Electron Microscope) for Failure Analysis and Metrology

10.31399/asm.cp.istfa2002p0077 ◽

2002 ◽

Author(s):

William E. Vanderlinde

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Material Structure ◽

Scanning Transmission Electron ◽

Transmission Electron ◽

Scanning Transmission ◽

Scanning Electron ◽

Thin Samples

Abstract Recent developments in transmission electron microscopy (TEM) sample preparation have greatly reduced the time and cost for preparing thin samples. In this paper, a method is demonstrated for viewing thin samples in transmission in an unmodified scanning electron microscope (SEM) using an easily constructed sample holder. Although not a substitute for true TEM analysis, this method allows for spatial resolution that is superior to typical SEM imaging and provides image contrast from material structure that is typical of TEM images. Furthermore, the method can produce extremely high resolution x-ray maps that are typically produced only by scanning transmission electron microscope (STEM) systems.

Fabrication of g-C3N4/Y-TiO2 Z-scheme heterojunction photocatalysts for enhanced photocatalytic activity

New Journal of Chemistry ◽

10.1039/d1nj03691b ◽

2021 ◽

Author(s):

SongSik Pak ◽

KwangChol Ri ◽

Chenmin Xu ◽

Qiuyi Ji ◽

Dunyu Sun ◽

...

Keyword(s):

Electron Microscopy ◽

Fourier Transform ◽

Photocatalytic Activity ◽

Electron Microscope ◽

Photoelectron Spectroscopy ◽

X Ray Diffraction ◽

X Ray ◽

Transmission Electron ◽

The g-C3N4/Y-TiO2 Z-scheme heterojunction photocatalysts were successfully synthesized. The powder X-ray diffraction, scanning electron microscopy, high-resolution transmission electron microscope, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy were used for...

Electron Tomography of HEK293T Cells Using Scanning Electron Microscope–Based Scanning Transmission Electron Microscopy

Microscopy and Microanalysis ◽

10.1017/s1431927612001158 ◽

2012 ◽

Vol 18 (5) ◽

pp. 1037-1042 ◽

Cited By ~ 1

Author(s):

Yun-Wen You ◽

Hsun-Yun Chang ◽

Hua-Yang Liao ◽

Wei-Lun Kao ◽

Guo-Ji Yen ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Scanning Transmission Electron Microscopy ◽

Electron Tomography ◽

Transmission Electron ◽

Scanning Transmission ◽

3D Volume ◽

AbstractBased on a scanning electron microscope operated at 30 kV with a homemade specimen holder and a multiangle solid-state detector behind the sample, low-kV scanning transmission electron microscopy (STEM) is presented with subsequent electron tomography for three-dimensional (3D) volume structure. Because of the low acceleration voltage, the stronger electron-atom scattering leads to a stronger contrast in the resulting image than standard TEM, especially for light elements. Furthermore, the low-kV STEM yields less radiation damage to the specimen, hence the structure can be preserved. In this work, two-dimensional STEM images of a 1-μm-thick cell section with projection angles between ±50° were collected, and the 3D volume structure was reconstructed using the simultaneous iterative reconstructive technique algorithm with the TomoJ plugin for ImageJ, which are both public domain software. Furthermore, the cross-sectional structure was obtained with the Volume Viewer plugin in ImageJ. Although the tilting angle is constrained and limits the resulting structural resolution, slicing the reconstructed volume generated the depth profile of the thick specimen with sufficient resolution to examine cellular uptake of Au nanoparticles, and the final position of these nanoparticles inside the cell was imaged.

Electron Microscopy and X-Ray Microanalysis in Forensic Science

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/56.4.930 ◽

1973 ◽

Vol 56 (4) ◽

pp. 930-943

Author(s):

John L Brown ◽

James W Johnson

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Chemical Elements ◽

Optical Microscope ◽

Forensic Analysis ◽

Depth Of Focus ◽

Crystalline Materials ◽

X Ray ◽

Transmission Electron ◽

Abstract The optical microscope has long been an important tool in forensic analysis for the comparison of firearms markings and the examination and identification of other minute bits of evidence. The electron microscope permits the examination of even smaller details and offers analytical capabilities unique to the type of instrument used. The transmission electron microscope can be used to identify very small amounts of crystalline materials through the process of electron diffraction. The scanning electron microscope can frequently supersede the optical microscope because of its superior depth of focus and range of magnification. When it is equipped with an energy dispersive X-ray analyzer, most of the chemical elements in a sample can be determined. Applications of these instruments have provided some interesting and instructive results in forensic analysis.

Two-Step Fabrication of Nano-PbS on Peacock Feathers Inspired by a Hair-Dyeing Method Used in Ancient Egypt

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.364.737 ◽

2013 ◽

Vol 364 ◽

pp. 737-741

Author(s):

Xiao Wei Liu ◽

Jia Jun Gu ◽

Fang Yu Zhang

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Ancient Egypt ◽

X Ray Diffraction ◽

X Ray ◽

Whole Process ◽

Transmission Electron ◽

Dyeing Method ◽

A rapid method towards nanoPbS on peacock feathers was reported and this is inspired by a hair-dyeing technology used in Ancient Egypt thousands of years ago. Original peacock feather was sulfhydrylated by 2, 3-dimercaptosuccinic acid (DMSA) dissolved in alcohol to enhance reaction sites, and then was immersed in the saturated PbO solution in calcium hydroxide and got the PbS peacock feather. The whole process is only two steps and could be completed within two hours. The morphology and structures of the sample were measured by the X-ray diffraction (XRD), Scanning electron microscopy (SEM), and transmission electron microscope (TEM) and results showed that the structure of original peacock feather was well duplicated. Compared with previous works, this method is faster and more efficient and thus has potentials to fabricate other functional sulfides.