scholarly journals Simulation of Acute Destructive Pancreatitis with Damage to the Adjacent Adipose Tissue in Pigs

2020 ◽  
Vol 13 (3) ◽  
pp. 233-240
Author(s):  
Imaeva Alfiya ◽  
Tagir Mustafin ◽  
Bazekin Georgiy ◽  
Radmir Rakhimov ◽  
Ildar Sharifgaliev

The aim of the study was to develop a model of acute destructive pancreatitis with damage to the extra-organ adipose tissue. Materials and methods. Simulation of acute destructive pancreatitis with damage of the extra-organ adipose tissue was performed in 22 white breed boars aged 3 months. The animals were divided into groups in accordance with two series of studies, where the control (first) group included 15 animals with the pancreas and extra-organ adipose tissue remained intact. Acute destructive pancreatitis was simulated in 7 pigs of the experimental (second) group based on the proposed technique (patent for an invention No. 2668201). Ultrasound examination of the abdominal cavity and retroperitoneal region was applied to confirm the development of expected pathology in experimental animals. Intensity of blood supply was assessed using contrast radiography by injecting a contrast medium into the superior mesenteric artery. Complete blood count and biochemical assay were performed. In addition, during autopsy tissue samples were taken for histological and immunohistochemical investigations to assess changes in the internal organs. Results. Acute destructive pancreatitis with damage to the extra-organic adipose tissue was regarded as achieved when clinical manifestations of apathy, lethargy, feed refusal were registered in animals. Biochemical blood tests revealed a significant increase in the concentration of alkaline phosphatase and alpha-amylase enzymes. Nitrogenous toxins, in particular, creatinine, were accumulated in the body of the experimental animals. Complete blood count revealed leukocytosis, the fact supporting the onset of the expected inflammatory process in the pancreas and adjacent adipose tissue. The development of necrotic and inflammatory processes in the organ was evidenced by a sharp increase in the level of amylase, creatinine and alkaline phosphatase in the blood of animals. Ultrasound examination revealed a picture of the blurring of pancreatic contours, heterogeneity of the organ structure, increased echogenicity, and enlargement of the duct. Intensification of the vascular pattern and increase in pancreatic contours were detected during the contrast examination of the abdominal vascular system. At autopsy, there were signs of enzymatic peritonitis, namely, serous-hemorrhagic exudate in the abdominal cavity and the cavity of the omental bursa, increased pneumatization of the intestinal tube with a thinning of its wall and foci of small hemorrhages. The pancreatic tissue in all cases was edematous, full-blooded, with multiple foci of fatty necrosis and purulent inflammation. Similar changes were found in the adjacent adipose tissue. At the light-optical level, vascular congestion, small foci of steatonecrosis and micro-abscesses were determined in the pancreas. Immunohistochemical studies largely evidenced the onset of acute destructive pancreatitis with damage to the extra-organ adipose tissue.Conclusion. Thus, the proposed technique allows simulating acute destructive pancreatitis with damage to the extra-organ adipose tissue in pigs, the event frequently occurring in the clinical practice. In animals the disease is accompanied by clear clinical manifestations correlating with findings of instrumental and laboratory investigations, and autopsy results. Investigations of the pancreatic and extra-organ adipose tissue samples at the light-optical and molecular levels evidence the fact that the authors have simulated a model of acute destructive pancreatitis with involvement of the adipose tissue adjacent to the organ in the pathological process.

2020 ◽  
Vol 10 (1) ◽  
pp. 35-42
Author(s):  
Yurii Yu. Sokolov ◽  
Artem M. Efremenkov ◽  
Aleksandr P. Zykin ◽  
Elena L. Tumanova ◽  
Zhanna R. Omarova ◽  
...  

Introduction. Gastric duplication cyst is a rare clinical observation. More often, these cysts are localized in the fundus or body of the stomach and have a common muscle layers and blood supply. Even more rarely, duplication cysts are not anatomically connected to the stomach and are located in other parts of the abdominal cavity or in the retroperitoneal space. Cystic duplication of the gastrointestinal tract, which are localized in the pancreas, is extremely rare. The aim of the study is to demonstrate the possibility of using laparoscopy for this disease. Materials and methods. The paper describes three rare clinical observations of gastric duplication cyst topographically associated with the pancreas. In the first clinical observation, gastric duplication cyst was combined with mediastinal duplication cysts, an esophageal bronchial fistula, and extralobar pulmonary sequestrations. In the second observation, a duplication cyst had communication with the pancreatic duct system and was clinically manifested by recurrent bleeding. In the third case, a duplication cyst is diagnosed behind the body and tail of the pancreas. Results. In all cases, surgical treatment was carried out by the laparoscopic method, leading to complete recovery. Histological examination in all the described observations confirmed the gastric type of epithelium of the mucous membrane of the cysts. The article provides a review of the literature. Conclusion. Thus, the efficiency of laparoscopic interventions in children with gastric duplications topographically associated with the pancreas was demonstrated. The described rare clinical manifestations, a combination of defects, as well as the possible presence of heterotopy of the mucous membrane of the duplication cyst confirm the need for surgical correction of the disease.


2021 ◽  
Vol 36 (3) ◽  
pp. 199-203
Author(s):  
Michael Kraemer ◽  
Cornelius Hess ◽  
Alexandra Maas ◽  
Burkhard Madea ◽  
Andras Bilkei-Gorzo ◽  
...  

Abstract Objectives In a previous investigation we searched for the occurrence of palmitic acid ester compounds of delta9-tetrahydrocannabinol (THC) and its primary metabolite 11-hydroxy-delta9-THC (11-OH-THC) in human body fluids and tissues (THC palmitic acid monoester [THC-Pal] and 11-OH-THC palmitic acid diester [11-OH-THC-DiPal]). As those esters could not be detected in various human body fluids (e.g. blood) or tissues (e.g. adipose tissue) we extended the investigation analyzing adipose tissue samples of mice previously given synthetic THC or a cannabis extract. Methods In total, 48 adipose tissue samples previously tested positive for THC by means of a liquid chromatographic triple quadrupole mass spectrometric (LC-QQQ-MS) method were analyzed for the presence of THC-Pal and 11-OH-THC-DiPal by means of LC-QQQ-MS. Results THC-Pal and 11-OH-THC-DiPal were not detected among the adipose tissue samples analyzed despite the presence of high THC concentrations within the adipose tissue. THC concentrations in adipose tissue were in a range of approximately 7–2,595 ng/g (median: 468 ng/g, average: 704 ng/g). Conclusions A (site-specific) synthesis of 11-OH-THC palmitic acid monoesters (11-hydroxy-delta9-THC-1-palmitate and 11-palmitoyloxy-delta9-THC) still remains to be done. After synthesis of these monoesters, their presence in the body fluids and tissues after THC administration should be investigated.


2018 ◽  
Vol 17 (2) ◽  
pp. 31-36
Author(s):  
V. O. Krylyuk ◽  
V. K. Hrodetsʹkyy ◽  
S. O. Sokolʹnyk ◽  
O. V. Fedosyeyeva ◽  
H. Yu. Tsymbalyuk

The aim of the study was to study the possibility of using a hemostatic biological haemostatic to stop bleeding from the parenchymal organs of the abdominal cavity using the surgical method of Damage control. The experiment was performed on laboratory rats that were divided into three groups: a control group, a group where, after injury, the liver wound was sutured with standard sutures and a group where the wound was plastered with a biological hemostatic agent. In order to determine the operational stress, we studied the enzymatic link of antioxidant protection - the level of catalase and superoxide dismutase. To study the indices in all experimental animals, the following tissues were taken: the lower lobe of the right lung, the proportion of the liver where the wounds were applied, the gastrocnemius muscle below the harness and the right kidney. The control points of the study were 1, 3 and 7 days after injury. During working with experimental animals, they adhered to all ethical norms established by international rules. The effect of operational stress on the enzymatic component of antioxidant protection in the tissues of the kidneys, liver, lungs and muscles was studied in the study of the combined trauma of the abdominal cavity complicated by massive bleeding and ischemic-reperfusion syndrome of the extremity. The changes in the indices of superoxide dismutase and catalase were studied. The following result was obtained: tamponing the liver wound with chitosan was a quick and effective means of temporary bleeding stop. At the same time, the duration of the operation was reduced from 19.8 to 12.5 minutes (by 36.8%) (p <0.05). Also, the level of catalase and superoxide dismutase was significantly lower in the group where the chitosan of the liver injury was used in comparison with the study group where standard stitches were applied. The deviations, which are determined in lung, liver, kidney and muscle tissues in RG-1 are evidence of the depth of their lesion and dysfunction, is a favorable factor for the further development of the triggering mechanisms of the systemic response of the body to inflammation and multiple organ failure. In contrast to the RG-2 rapid development of the systemic response was avoided. We can assume that ischemic-reperfusion syndrome is a stimulating factor in the development of systemic changes, although this statement requires further proof. It is also important to further prospective study of this topic in terms of the following questions: what effect has chitosan on liver tissue with prolonged exposure (more than 2 days) and what effect does additional injection of infusion solutions have as an agent for the treatment of hypovolemic shock.


2011 ◽  
Vol 56 (No. 11) ◽  
pp. 577-580 ◽  
Author(s):  
Z. Adamiak ◽  
A. Pomianowski ◽  
M. Kwiatkowska ◽  
A. Majchrowski ◽  
H. Matyjasik ◽  
...  

This article describes the procedure of ventriculo-peritoneal shunt implantation in an English bulldog with laparoscopy-assisted placement of the peritoneal catheter in the abdominal cavity. Prior to surgery, the patient was subjected to physical and neurological examinations involving a complete blood count (CBC), biochemistry profiling, EEG and MRI. This case report also describes the patient&rsquo;s pharmacological treatment before the procedure, the applied surgical technique and the benefits of the laparoscopy-assisted approach. &nbsp;


2021 ◽  
Vol 74 (10) ◽  
pp. 2374-2378
Author(s):  
Andriy Bambuliak ◽  
Nataliia Kuzniak ◽  
Valentyna Honcharenko ◽  
Marianna Ostafiychuk ◽  
Alina Palamar

The aim: Determining the ability of samples based on MMSC – AT differentiating in the osteogenic direction. Materials and methods: The study was conducting at Bukovinian State Medical University, Chernivtsi, Ukraine. Adipose tissue samples were obtaining from the neck of 60 experimental animals (white Wistar rats). Multipotent mesenchymal stromal cells of adipose tissue were obtained by grinding adipose tissue of rats in 0.1% collagenase 1A . Alkaline phosphatase activity was assessing by using the Alkaline Phosphatase Detection Kit (Sigma, USA) according to the manufacturer’s protocol. Osteopontin gene expression was determining by immunocytochemical method. To determine the mRNA used the PCR method, which is associated with reverse transcription (RT-PCR) in the area of quantification of gene expression to the marker BGP. Results: On the 21st day of observations, the expression of mRNA encoding the BGP gene decreased in samples № 1 and № 3 to 35,800 ± 420.0 copies and to 35,000 ± 400.0 copies, p1<0.01, p>0.05. Also was observing growth of copies of the BGP gene in samples № 2 and № 4 in 2.1, р<0.01 and 2.2 times, р-р2<0.05, relative to the data in sample № 1. Conclusions: Comparative study of osteoplastic properties samples MMSC-AT showed that a larger number of cells differentiate into the osteoblasts in samples containing MMSC-AT + PRP (№ 2) and MMSC-AT + PRP + «Kolapan» (№ 4). This has been proven higher alkaline phosphatase activity, higher levels osteopontin expression, and higher levels BGP gene expression.


2021 ◽  
Vol 49 ◽  
Author(s):  
Giovana Scuissiatto De Souza ◽  
Gabriela Oliveira da Paz Augusto Pinto ◽  
Weslley Junior De Oliveira ◽  
Rosangela Locatelli-Dittrich

Background: Acute lymphoblastic leukemia (ALL) is a malignant neoplasia in which there is proliferation of lymphoid progenitor cells in the bone marrow, blood, and extramedullary sites. This disorder has a fast and progressive development; in dogs, cases of infiltration of ALL cells in the central nervous system (CNS) are uncommon and rare. Diagnosis can be achieved with the help of the clinical history and physical, radiographic, hematological, myelographic, and cerebrospinal fluid (CSF) tests in patients with or without neurological clinical signs. The present report aims to describe a case of ALL and the presence of lymphoblasts in the CSF of a dog with neurological clinical signs.Case: An 8-year-old Lhasa Apso dog was examined at the Veterinary Hospital of Universidade Federal do Paraná, Curitiba campus. At the physical examination, the animal exhibited apathy and paralysis of pelvic limbs, which progressed to tetraplegia. Abdominal palpation revealed presence of hepatosplenomegaly and absence of lymphadenomegaly. No alterations were observed in radiographs of the cervical, thoracic or lumbar spine. A complete blood count revealed presence of non-regenerative anemia (hematocrit = 22%), extreme lymphocytosis (185,229 cells/µL), lymphoblasts at a level of 72% (133,364 cells/µL), and thrombocytopenia (66,000 platelets/µL). The biochemical tests revealed increased alkaline phosphatase (859 IU/L). The levels of alanine aminotransferase, creatinine, urea, total protein, albumin, and globulin were normal. The diagnosis of ALL was achieved with the help of a myelogram. The myelogram findings included 39% of mature lymphocytes and 59% of lymphoblasts exhibiting large size, spherical shape, poorly delimited borders, with a high nucleus/cytoplasm ratio, marked cytoplasmic basophilia, and 2 to 3 evident nucleoli; metarubricytes (1%) and promyelocytes (0.6%) were also observed. The CSF contained an increased number of nucleated cells (27 cells/µL) comprising lymphocytes (43%), macrophages (33%), and segmented neutrophils (24%). Of the 11.6 lymphocytes per µL of CSF, 8.1 were lymphoblasts, which indicates infiltration of ALL cells in the CNS. The animal died one day after collection of bone barrow and CSF. Discussion: Relevant alterations observed in this case included the neurological signs caused by the infiltration of neoplastic cells in the CNS, severe leukocytosis and lymphocytosis, with large amounts of lymphoblasts in the blood and predominance of lymphoblasts in the bone marrow, which are alterations typically found in ALL. The animal also exhibited non-regenerative anemia and thrombocytopenia, which were secondary to infiltration of leukemic cells in the bone marrow. The CSF exhibited pleocytosis (27 cells/ µL), and 30% of the cells observed were lymphoblasts. Lymphoblast infiltration in the CNS of leukemic dogs is rare, and other studies have reported absence of neurological signs or neurological signs different from those observed in the present study. CSF analysis in indicated in cases of leukemia to assess leukemic cell infiltration in the CNS. In the case reported here, the plasma level of alkaline phosphatase was increased (859 IU/L) as a consequence of hepatomegaly and hepatic cholestasis. ALL is a very aggressive, proliferative neoplasia, and the resulting lymphoblasts infiltrated the CNS of the animal. In cases of ALL, performing complete blood count, myelogram, and CSF analysis is indicated whether the patients exhibit neurological signs or not.


2020 ◽  
Vol 16 (3) ◽  
pp. 202-206
Author(s):  
Jung Han Lim ◽  
Sue Min Kim ◽  
Seungyun Jee ◽  
Seong Oh Park

An 84-year-old male patient with recurrent cellulitis in the left submandibular area visited the outpatient clinic. He underwent debridement of the wound with administration of intravenous antibiotics. The defect was reconstructed using a split-thickness skin graft. After 2 months, the cellulitis recurred adjacent to the prior lesion in the submandibular area. The wound improved with intravenous antibiotics and conservative treatment. During the second hospitalization, the patient was evaluated for abnormal complete blood count, and was finally diagnosed with myelodysplastic syndromes. Recurrent cellulitis in this patient was not accompanied with any other symptoms; instead of being a simple uncontrolled infection it had a systemic origin. Physicians should consider the possibility of systemic causes for recurrent cellulitis, such as myelodysplastic syndromes, especially if laboratory results suggest pancytopenia.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Michael Kraemer ◽  
Cornelius Hess ◽  
Alexandra Maas ◽  
Burkhard Madea ◽  
Andras Bilkei-Gorzo ◽  
...  

Abstract Objectives In a previous investigation we searched for the occurrence of palmitic acid ester compounds of delta9-tetrahydrocannabinol (THC) and its primary metabolite 11-hydroxy-delta9-THC (11-OH-THC) in human body fluids and tissues (THC palmitic acid monoester [THC-Pal] and 11-OH-THC palmitic acid diester [11-OH-THC-DiPal]). As those esters could not be detected in various human body fluids (e.g. blood) or tissues (e.g. adipose tissue) we extended the investigation analyzing adipose tissue samples of mice previously given synthetic THC or a cannabis extract. Methods In total, 48 adipose tissue samples previously tested positive for THC by means of a liquid chromatographic triple quadrupole mass spectrometric (LC-QQQ-MS) method were analyzed for the presence of THC-Pal and 11-OH-THC-DiPal by means of LC-QQQ-MS. Results THC-Pal and 11-OH-THC-DiPal were not detected among the adipose tissue samples analyzed despite the presence of high THC concentrations within the adipose tissue. THC concentrations in adipose tissue were in a range of approximately 7–2,595 ng/g (median: 468 ng/g, average: 704 ng/g). Conclusions A (site-specific) synthesis of 11-OH-THC palmitic acid monoesters (11-hydroxy-delta9-THC-1-palmitate and 11-palmitoyloxy-delta9-THC) still remains to be done. After synthesis of these monoesters, their presence in the body fluids and tissues after THC administration should be investigated.


2017 ◽  
Vol 45 ◽  
pp. 5
Author(s):  
Camila Gottlieb Lupion ◽  
Eloisa Helena Moreira Pino ◽  
Elissandra Da Silveira. ◽  
Carine Ribas Stefanello ◽  
Leticia Talita Baretta ◽  
...  

Background: Pemphigus foliaceus is an autoimmune skin disease whose the occurrence in feline clinic is uncommom. The etiology is unknown in most cases. Althought it is a rare condition in cats, pemphigus foliaceus can be induced by drugs. It may affect both adult and old cats with no sexual or racial predilection and diagnosis is based on animal historical, clinical examination and skin histopathology. Moreover, the treatment consists in immunosuppressive-based drugs. This paper describes a pemphigus foliaceus case in an eight-month-old kitten, probably due a cutaneous adverse drug reaction.Case: A 8-month-old, neutered male mongrel cat with crusted lesions located on face was examinated at the Veterinary Hospital. The owners reported that they had adopted the patient two months before the clinical presentation suggesting that this background was unknown. The cat was treated in a local veterinary clinic with antibiotics and anti-inflammatory for rhinotracheitis and herpetic dermatitis on face due to the occurrence of sneezing and crusted pruriginous dermatitis. The kitten presented erosions, purulent exudation and crusts on pinnae, nasal and supra ocular areas, paronychia with purulent exudate and alopecia at distal portion of the tail. Supplementary exams such as skin scraping, cytology and fungal culture were performed to rule out other agents and confirm the diagnosis. The parasitological and fungal examination resulted negative despite the fact that cytological exam showed coccoid bacteria. Complete blood count and biochemical profile showed no alteration and the test for feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) resulted negative. Histological changes were consistent with intra-epidermal pustular subcorneal with acantholysis dermatitis. An immunosuppressive dose of prednisolona was prescribed for 15 days. After this time, the crusted lesions were substituted by alopecia areas. Every 20 days the corticosteroid dose was reduced by half and after 90 days, the patient showed repilation in all regions previously affected during 545 days whithout treatment.Discussion: Pemphigus foliaceus is usually an idiopathic disease but in some cases it may be induced by drugs and infectious or parasitic agents. Previous reports described that animals younger than 1 year-old and older than 17 year-old can be affected. However, the disease occurrence is rare in young cats. The age of onset of symptoms was early considering that the literature describes 5 years of age as an average for the starting of the disease. Pemphigus foliaceus is considered a pustular disease. However, pustule is rarely reported in cats in contrast with crusted lesions which are the most common clinical sign. The body regions affected were the same as reported in the literature with the addition of systemic signs as anorexia and lethargy. Fungal culture and parasitological examination should always be performed in cats with clinical evidence to discharge other agents. Diagnosis is made by skin histopathology in which acantholytic cells are the main characteristic. The importance of the differential diagnosis and skin biopsy regardless of age enabled the diagnosis and therapeutic success. It is suggested that Pemphigus foliaceus was of pharmacodermic etiology because of the age of the patient, a history of administration of unknown antimicrobial medication and the lack of recurrence of clinical manifestations after 545 days of the end of glucocorticoid treatment


Author(s):  
Daniel E. Figueroa ◽  
Gustavo Macchi ◽  
Manuel Haimovici

Congrids inhabit the warm and temperate seas of the world, from the coastline to the slope of various continents. Many of its species are much valued by commercial and artisan fishing; nevertheless, at world level, there is great ignorance about the life cycle of the members of this genus. Some species of the genus, such as those from the North Atlantic, appear to be semelparous, for they cease feeding, decalcify, loose their teeth and migrate to deep waters to spawn. Specimens of the southern conger eel Conger orbignianus were examined for the purpose of learning about their biology in waters of the western South Atlantic. Histological sections were analysed from ovaries of coastal conger specimens from the North Argentina coastal littoral, the Argentine Sea deep waters, and the giant congers from southern Brazil. Two oocitary generations have been observed in coastal females typical of total spawners, with a previtellogenetic batch that does not reach maturity, and a vitellogenetic batch that does it together with its size, completely surrounded by adipose tissue. The ovaries of fish captured in deep waters occupied the whole abdominal cavity, with scarce adipose tissue and degraded body. The giant congers from southern Brazil were captured in very deep waters, healthy, with few atretic oocytes and abundant adipose tissue. Evidence of semelparity was found in the southern conger eel; the asistosis stage, the body degradation during the reproductive migration and gigantism might be common attributes in other species of the genus.


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