scholarly journals Effect of Electron Beam Irradiation on Viability of Sarcocystis spp. in Beef

Author(s):  
G. Eslami ◽  
S. Peletto ◽  
M. Vakili ◽  
S. Kargar

Background: Sarcocystosis is one of the most distributed parasitic diseases over the world, caused by Sarcocystis spp. In this study, we assessed the effect of electron beam irradiation on the viability of Sarcocystis spp. in beef.  Methods: Experimental beef groups were irradiated by four different electron beam doses of 1, 2, 3, and 4 kGy, at intervals of 0 and 24 h after irradiation, then the samples were transferred inside a sterile microtube containing RNAlater solution and stored at -20 °C till next steps. RNA extractions and cDNA synthesis were done using the related kit in order to detect the presence of the 18S ribosomal RNA region. Relative quantification was carried out using SYBR Green Real time Polymerase Chain Reaction. The statistical analysis was done using SPSS 16.0 by Tukey’s and Kruskal-Wallis tests. Results: Irradiation at zero time was not effective on viability of Sarcocystis, but at 24 h, irradiation doses of 3 (p=0.003) and 4 kGy (p=0.008) caused a significant reduction in Sarcocystis viability. Irradiation doses of 1 and 2 kGy had no significant (p>0.05) effect on Sarcocystis viability reduction. Also, no significant differences (p>0.05) were observed between irradiation doses of 3 and 4 kGy. Conclusion: Electron beam radiation at dose of 3 kGy was effective as the optimal dose for the elimination of Sarcocystis spp. in beef. 

2014 ◽  
Vol 1073-1076 ◽  
pp. 86-89
Author(s):  
Ming Hong Wu ◽  
Jia Ling Li ◽  
Xiang Xin He ◽  
Yan Feng Sun ◽  
Gang Xu ◽  
...  

The irradiation degradation of selected estrogens quinestrol (QS) , norethindrone (NET) and dienestrol (DS) were evaluated by electron beam . Processes using electron beam can efficiently degradate QS, NETand DS, usually at reaction dosage lower than 6KGy. Especially remarkable is the high degradation efficiency shown by oxygen saturation which is extremely favored by acidic environment. The order of the degradation rate: DS > NET> QS by different analysis methods such as HPLC. Among electron beam irradiation carried out, higher removal of 90% from solution was observed. In addition, the estrogenic activity of QS, NET and DS was carried out by rotifer test.


2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Jae Sung Jeon ◽  
Do Hung Han ◽  
Boo Young Shin

In this study, we blended poly(ε-caprolactone) (PCL) into poly(lactic acid) (PLA) and compatibilized these PLA/PCL blends by electron-beam irradiation in the presence of a reactive agent to overcome drawbacks of PLA. To produce compatibilized blends, mixtures of the PLA/PCL/reactive agent were prepared using a twin-screw extruder and exposed to electron-beam radiation at room temperature. Glycidyl methacrylate (GMA) was chosen as the reactive agent to achieve interfacial cross-copolymerization between PLA and PCL phases. Morphological, rheological, and mechanical properties and biodegradabilities of blends were investigated. The morphological study showed significantly improved interfacial adhesion for compatibilized blends, and this was supported by FTIR analysis and a rheological study. Impact strengths, thermal stabilities, and rheological properties of PLA/PCL blends were improved by compatibilization, and the biodegradabilities of compatibilized PLA/PCL blends were greater than that of pure PLA.


2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Jae Baek Lee ◽  
Young-Gwang Ko ◽  
Donghwan Cho ◽  
Won Ho Park ◽  
Byeong Nam Kim ◽  
...  

Biodegradable poly(lactide-co-glycolide) (PLGA) has found widespread use in modern medical practice. However, the degradation rate of PLGA should be adjusted for specific biomedical applications such as tissue engineering, drug delivery, and surgical implantation. This study focused on the effect of electron beam radiation on nanofibrous PLGA mats in terms of physical properties and degradation behavior with cell proliferation. PLGA nanofiber mats were prepared by electrospinning, and electron beam was irradiated at doses of 50, 100, 150, 200, 250, and 300 kGy. PLGA mats showed dimensional integrity after electron beam irradiation without change of fiber diameter. The degradation behavior of a control PLGA nanofiber (0 kGy) and electron beam-irradiated PLGA nanofibers was analyzed by measuring the molecular weight, weight loss, change of chemical structure, and fibrous morphology. The molecular weight of the PLGA nanofibers decreased with increasing electron beam radiation dose. The mechanical properties of the PLGA nanofibrous mats were decreased with increasing electron beam irradiation dose. Cell proliferation behavior on all electron beam irradiated PLGA mats was similar to the control PLGA mats. Electron beam irradiation of PLGA nanofibrous mats is a potentially useful approach for modulating the biodegradation rate of tissue-specific nonwoven nanofibrous scaffolds, specifically for soft tissue engineering applications.


2021 ◽  
Vol 11 (1) ◽  
pp. 26-34
Author(s):  
Thu Hong Pham Thi ◽  
Thanh Duoc Nguyen ◽  
Thi Ly Nguyen ◽  
Nhut Khanh Chu ◽  
Van Chung Cao ◽  
...  

Vietnamese graphite powder was irradiated by electron beam radiation (EB) at a range of dose from 0 to 120 kGy, then the graphite samples were expanded with a mixture of H2O2:H2SO4 (1,4:20, v/v) incorporating microwave treatment at 700 W for 30 seconds. The characteristic properties of graphite before and after expansion were evaluated by methods of FTIR, Raman, SEM and XRD. Besides the self-assembled of the graphite’s structure after irradiation, the electron beam radiation also facilitated intercalation processing to make expanded graphite, with coefficient of expansion (Kv) 35% higher than that of pristine graphite.


2021 ◽  
Vol 2112 (1) ◽  
pp. 012005
Author(s):  
Haifeng Qi ◽  
D Sporea ◽  
A Stancalie ◽  
D Ighigeanu ◽  
D Neguţ ◽  
...  

Abstract This paper reports the comparative experimental study concerning the irradiation effects of gamma-ray and electron beam on fiber Bragg grating (FBG) and distributed feedback fiber laser (DFB-FL). The obvious reflection wavelength shifts are observed for FBGs and DFB-FLs without evident changes in reflectivity and bandwidth under the current experimental irradiation condition, up to 60 kGy gamma radiation and 100 kGy electron beam radiation, respectively. Especially for DFB-FLs, evident attenuation in output power is observed and the rising tendency of the attenuation under increasing irradiation dose is demonstrated as well. Thus, the DFB-FLs are more suitable for radiation detection as compared to passive FBGs.


2020 ◽  
Vol 865 ◽  
pp. 19-24
Author(s):  
Shane C. Halligan ◽  
Kieran A. Murray ◽  
Olivier Vrain ◽  
John G. Lyons ◽  
Luke M. Geever

Exposing smart materials to electron beam radiation can induce free radical reactions, such as chain branching or crosslinking, hence enhancing the characteristics of the polymers. Poly (N-vinylcaprolactam) (PNVCL) is a smart material which was synthesised by photopolymerisation. Subsequently, samples were exposed to electron beam technology, where electron beam irradiation was utilised in a novel approach. This led to the modification of the rheological and phase transition properties. Modifying PNVCL through electron beam irradiation opens new avenues and potential applications in the biomedical field. Physically cross-linked PNVCL polymers were prepared by photopolymerisation and samples were subsequently irradiated at different dose ranges (5kGy, 25kGy and 50 kGy). The rheological properties of the PNVCL based samples were established by rheological analysis. Similarly, the PNVCL based sample polymers were further characterised in solution to determine the phase transition of PNVCL.


2020 ◽  
Vol 1002 ◽  
pp. 57-65
Author(s):  
Abdulkader M. Alakrach ◽  
Nik Noriman Zulkepli ◽  
Awad A. Al-Rashdi ◽  
Sam Sung Ting ◽  
Rosniza Hamzah ◽  
...  

This study aimed to develop novel Polylactic acid/ Halloysite (PLA/ HNTs) films which showed better properties when they were used for food packaging. They also displayed better mechanical, barrier, morphological and structural properties when the researchers analysed the impact of the electron beam irradiation on the nanomaterials. They prepared PLA-based nanocomposites containing 5 % w/w of HNTs using the solution casting process. These nanocomposites were further exposed to different ebeam doses (i.e., 0, 20, 40 and 60 kGy). The researchers assessed the effect of the electron beam irradiation on the various properties of the PLA. All the composites showed a homogenous dispersion and distribution of the HNTs in this PLA matrix. Results indicated that the nanocomposites showed better barrier properties in comparison to the neat PLA. Furthermore, the ebeam irradiation could increase the glass-transition temperature and lead to the development of more crosslinks, which increased the degradation temperature and hydrophilicity of the nanocomposites. In this study, the researchers showed that the PLA/HNTs films were effective materials that could be used for the electron beam processing of the pre-packed foods. The best effect was noted for the 20 kGy dosage which was used in the study.


Author(s):  
Nguyen Dang My Duyen ◽  
Pham Ngoc Viet

Electron beam irradiation is one of the most effective starch modification methods. Moisture is one of the factors that is affected by the irradiation via the free radical formation. The effect of the moisture content on the technological properties of irradiated starch samples was studied. The moisture content of corn starch samples in this study was adjusted at 9.08, 12.96, 15.06, 20.39 and 29.09% (w w). Corn starch samples were irradiated at 5 kGy. Color, free acid, solubility, swelling, transparency, syneresis and starch gel structure were studied to elucidate the effect of the moisture on technological properties of corn starch treated by electron beam. The results showed that electron beam radiation caused starch samples at different moisture to break into small molecules due to the separation of the glycosidie bonds. Thus, free acid, swelling, solubility of irradiated starch samples were higher than those of natural starch samples and were increased with low moisture content. The results also indicated that electron beam treatment increased the syneresis of irradiated starch samples. The texture profile analysis (TPA) of starch gel structure showed that textural properties such as hardness, elasticity and chewiness of irradiated starch samples were higher than those of natural starch samples.


Author(s):  
J. Liu ◽  
J. M. Cowley

It is well known that various electron beam induced effects can occur under high energy electron beam irradiation on the studied material in an electron microscope. It has been reported that under electron beam irradiation small metallic Ni particles can be reduced from the bulk NiO crystals and these Ni crystals are always in epitaxial relationship with the NiO substrate. These Ni particles may be formed by the preferential knock-out of oxygen atoms by the incident electrons and the subsequent aggregation of Ni atoms to form the nucleii. These nuclei will grow with further electron beam irradiation, forming the small Ni islands on the surface of the NiO crystals. Recently, we observed that not only does the reduction process occur on the NiO surface but also other kinds of interactions occur at the same time. First, large NiO crystals can be melted and flow away from the radiated area in an electron microscope. The process is radiation dose dependent.


2019 ◽  
Vol 69 (13) ◽  
pp. 1579-1582
Author(s):  
Marine H. Balayan ◽  
Astghik Z. Pepoyan ◽  
Anahit M. Manvelyan ◽  
Vardan V. Tsaturyan ◽  
Bagrat Grigoryan ◽  
...  

Abstract Purpose The implementation of electron beam radiation coupled with the use of probiotics is one of the newest food processing technologies that may be used to ensure food safety and improve shelf life of food products. The purpose of this study was to evaluate the effect of 50–150-Gy electron beam irradiation on the antimicrobial activity of the putative probiotic strain Lactobacillus rhamnosus Vahe. Methods Low-dose electron beam irradiation of lactobacilli cells was performed using the Advanced Research Electron Accelerator Laboratory’s electron accelerator, and the agar well diffusion method and Verhulst logistic function were used to evaluate the effect of radiation on anti–Klebsiella pneumoniae activity of the cell free supernatant of L. rhamnosus Vahe cells in vitro. Results Our results suggest that 50–150-Gy electron beam irradiation decreases the viability of the investigated lactobacilli, but does not significantly change the probiotic’s activity against K. pneumoniae. Conclusions Results indicate that the combined use of irradiation and L. rhamnosus Vahe might be suggested for non-thermal food sterilizing technologies.


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