scholarly journals Level of Bisphenol A in Follicular Fluid and Serum and Oocyte Morphology in Patients Undergoing IVF Treatment

2019 ◽  
Author(s):  
Seyedeh Mahsa Poormoosavi ◽  
Mohammad Amin Behmanesh ◽  
Sima Janati ◽  
Hosein Najafzadehvarzi
Keyword(s):  
Follicular Fluid ◽  
Germinal Vesicle ◽  
Sample Population ◽  
Serum Samples ◽  
Cross Sectional ◽  
Vitro Fertilization ◽  
Elisa Kit ◽  
Oocyte Morphology ◽  
The Mean

Objective: To assess the correlation between the levels of BPA in the serum and follicular fluid (FF) using oocyte morphology. Materials and methods: In this cross-sectional research, oocyte, FF, and serum samples were obtained from a sample population consisting of 90 women undergone in vitro fertilization in Ganjavian Hospital in Dezful, Iran during October 2017-March 2018. The ELISA kit was utilized for the measurement of the BPA levels. In addition, oocyte morphology simultaneous with inverted optical microscopy. Results: Follicular fluid BPA levels had no significant effect on MII oocytes (p ≥ 0.05). However, the mean levels of degenerated oocytes and germinal vesicle (GV) were significantly higher in the women with high BPA levels in the FF (p ≤ 0.05). Moreover, the mean counts of MII oocytes and oocytes were significantly higher in the women with serum BPA levels of ≤ 50 ng/ml (p ≤ 0.05), while the mean count of GV oocytes was significantly higher in the women with serum BPA levels of ≥ 150 ng/ml (p ≤ 0.05). Conclusion: According to the results, higher FF BPA levels were associated with the higher counts of GVs and oocytes, while oocytes with higher maturity can be achieved in lower levels of BPA in the serum of patients.

scholarly journals Factors Related to the Number of Antral Follicles on InVitro Fertilization (IVF)

2016 ◽  
pp. 75
Author(s):  
Fransiskus C Raharja ◽  
Ketut Suwiyoga ◽  
IPG Wardhiana
Keyword(s):  
Prevalence Ratio ◽  
Cross Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Vitro Fertilization ◽  
Antral Follicles ◽  
Chi Square Test ◽  
The Mean ◽  
Infertile Patients

Objective: To determine factors which are related to the number of antral follicles on infertile patients. Method: This cross sectional study was conducted in In-Vitro Fertilization (IVF) clinic of Graha Tunjung, Sanglah hospital, Bali. All fertile patients following the IVF program were calculated the number of antral follicles in both ovarian using transgene USG. This sample was recruited by random sampling from April 1st, 2001 to April 30th, 2011. We analyzed the data using Chi square test through SPSS for Windows 17.0 version. Result: Of 102 samples, the mean of patients’ age was 32.9% (SD 4.6) years old. From 72 patients (70.6%) experienced above 3 years of infertile period, the primary infertile was on 69 patients (67.7%). There was a relationship between patients’ age and the number of antral follicles significantly (prevalence ratio (PR) 1.41; 95% CI 1.11- 1.79). Meanwhile, the number of antral follicles and type of infertile (PR 1.02; 95% CI 0.76-1.37) also infertile period (PR 0.95; 95% CI 0.72-1.27) were not associated significantly. Conclusion: Patients’ age has an association with the number of antral follicles on IVF. [Indones J Obstet Gynecol 2016; 4-2: 75-77] Keywords: age, infertile, infertile period, number of antral follicles and type of infertile

scholarly journals Follicular and serum levels of vitamin D in women with unexplained infertility and their relationship with in vitro fertilization outcome: an observational pilot study

2021 ◽  
Vol 17 (5) ◽  
pp. 1418-1422
Author(s):  
Ana Jeremic ◽  
Zeljko Mikovic ◽  
Emina Sudar-Milovanovic ◽  
Esma Isenovic ◽  
Milan Perovic
Keyword(s):  
Vitamin D ◽  
Follicular Fluid ◽  
Serum Vitamin ◽  
Serum Levels ◽  
Unexplained Infertility ◽  
Cross Sectional ◽  
Vitro Fertilization ◽  
Ivf Outcomes ◽  
Serum Vitamin D

IntroductionFollicular and serum vitamin D are considered potential markers of the oocyte and embryos' quality and predictors of IVF outcomes.Material and methodsThis retrospective cross-sectional study correlated vitamin D in sera and follicular fluid of women with unexplained infertility mutually and with IVF outcomes. ELISA was used for measuring Vitamin D.ResultsResults show only a positive correlation between follicular and serum levels of Vitamin D (Rho=0.615, p=0.025) and between follicular levels of Vitamin D with the percentage of embryo fragmentation (Rho=0.544; p=0.036).ConclusionsResults suggests that serum and follicular fluid vitamin D measurements could be complementary tools to the routine assessment of embryos.

scholarly journals Effects of melatonin and human follicular fluid supplementation of in vitro maturation medium on mouse vitrified germinal vesicle oocytes: A laboratory study

2021 ◽  
pp. 889-898
Author(s):  
Razieh Doroudi ◽  
Zohre Changizi ◽  
Seyed Noureddin Nematollahi-Mahani
Keyword(s):  
Follicular Fluid ◽  
In Vitro Maturation ◽  
Germinal Vesicle ◽  
Cell Stage ◽  
Human Follicular Fluid ◽  
Vitro Fertilization ◽  
Treatment Groups ◽  
Ivf Outcomes

Background: Vitrification as the most efficient method of cryopreservation, enables successful storage of oocytes for couples who undergo specific procedures including surgery and chemotherapy. However, the efficacy of in vitro maturation (IVM) methods with vitrified germinal vesicle (GV) oocytes could be improved. Objective: As melatonin and follicular fluid (FF) might enhance IVM conditions, we used these supplements to assess the maturation rate of vitrified GV oocytes and their artificial fertilization rate. Materials and Methods: Four hundred mouse GV oocytes were harvested, vitrified, and assigned into control (C-Vit-GV) and treatment groups of melatonin (M-Vit-GV), human follicular fluid (HFF-Vit-GV), and a combination (M + HFF-Vit-GV). A non-vitrified group of GV oocytes (non-Vit-GV) and a group of in vivo matured metaphase II (Vivo-MII) oocytes served as control groups to evaluate the vitrification and IVM conditions, respectively. Maturation of GV oocytes to MII and further development to two-cell-stage embryos were determined in the different groups. Results: Development to two-cell embryos was comparable between the Vivo-MII and non-Vit-GV groups. IVM and in vitro fertilization (IVF) results in the non-Vit-GV group were also comparable with the C-Vit-GV oocytes. In addition, the IVM and IVF outcomes were similar across the different treatment groups including the M-Vit-GV, HFF-Vit-GV, M + HFF-Vit-GV, and C-Vit-GV oocytes. Conclusion: Employing an appropriate technique of vitrification followed by suitable IVM conditions can lead to reasonable IVF outcomes which may not benefit from extra supplementations. However, whether utilizing other supplementation formulas could improve the outcome requires further investigation. Key words: Vitrification, Germinal vesicle, In vitro oocyte maturation, Melatonin, Follicular fluid.

scholarly journals The effect of follicular fluid selenium concentration on oocyte maturation in women with polycystic ovary syndrome undergoing in vitro fertilization/Intracytoplasmic sperm injection: A cross-sectional study

2021 ◽  
pp. 689-698
Author(s):  
Seyedeh Mahsa Poormoosavi ◽  
Mohammad Amin Behmanesh ◽  
Hossein Najafzadeh Varzi ◽  
Shabnam Mansouri ◽  
Sima Janati
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Polycystic Ovary Syndrome ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Selenium Concentration ◽  
Ovary Syndrome ◽  
Cross Sectional ◽  
Vitro Fertilization

Background: A high level of free radicals and oxidative substances in women with polycystic ovary syndrome (PCOS) can affect the ovaries through oxidative stress. Antioxidants such as selenium, a vital trace element in human health, can improve the prognosis of PCOS by reducing oxidative stress. Objective: This study was performed due to the lack of comprehensive information about selenium concentration in follicular fluid and its effect on the oocyte count and quality in infertile women with PCOS. Materials and Methods: In this cross-sectional study, 78 women with PCOS referred to Umm-al-Banin Infertility Clinic Center, Ganjavian Hospital, Dezful, Iran for in-vitro fertilization from March to November 2019 were enrolled. After ovarian stimulation with the antagonist protocol, the oocytes were retrieved under transvaginal ultrasound in in-vitro fertilization/intracytoplasmic sperm injection cycles, and selenium concentrations were measured in the follicular fluid using an atomic absorption method by spectrophotometer device. Oocyte count and morphology were evaluated using inverted optical microscopy. Results: There were no significant differences between follicular fluid selenium concentrations in terms of the total number of oocytes and immature oocytes in the metaphase I and germinal vesicle stages. However, a significantly reduced number of metaphase II oocytes was observed at selenium levels < 40 μg/dL (p = 0.001). Conclusion: Based on our results, low levels of follicular selenium concentration in infertile women with PCOS can reduce the quality and potency of oocyte maturation. Key words: Polycystic ovary syndrome, Oxidative stress, Selenium, In vitro fertilization, Oocyte quality, Follicular fluid.

scholarly journals Depression among Infertile Women in Gaza Strip: Symptom Severity and Predictors

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Aymen Elsous ◽  
Sae’d Abu El-Kass ◽  
Akram Salama ◽  
Mahmoud Radwan ◽  
Soha Abo-Eid ◽  
...  
Keyword(s):  
Psychological Intervention ◽  
Gaza Strip ◽  
Wald Test ◽  
Depression Symptoms ◽  
Mild Depression ◽  
Cross Sectional ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
The Mean

Background. Mental disorders are expected for women suffering from infertility. Depression is a predictable consequence but requires more investigations and considerations. This study is aimed at determining the severity of depression symptoms and its predictors among infertile women in the Gaza Strip, Palestine. Materials and Methods. A cross-sectional study was conducted. Three hundred eighty-five infertile women participated and were selected by convenience sampling. The participated women were selected from three large and major in vitro fertilization treatment centers (Al Helo, Al Basma, and Hindawi). A validated Arabic version of the Beck Depression Inventory-II was used. Univariate and multivariate logistic regression was applied to determine potential predictors for depression symptoms, and p ≤ .05 was statistically significant. Results. The mean age of participated women was 29 ± 6.58 years, and the mean duration of marriage and infertility was 7.76 ± 5.31 and 5.43 ± 3.50 years, respectively. Half of the women had some form of depression symptoms (22.3%, 8.6%, and 10.6% showed to have mild depression, moderate, and severe depression symptoms, respectively). Predictors of depression symptoms were as follows: duration of marriage (Wald test: 10.493; CI95%: 0.248-0.774), at least one abortion (Wald test: 21.233; CI95%: 1.863–4.528), primary infertility (Wald test: 6.666; CI95%: 1.148–2.742), and husband cause of infertility (Wald test: 10.878; CI95%: 0.800–0.982). Conclusion. Infertility affects the various aspect of women’s life. Psychological intervention including counselling, support, and therapy would be necessary to limit the consequences of infertility. Such interventions could be implemented in infertility treatment clinics.

scholarly journals An Evaluation of the Use of G-CSF as an Adjunct to IVF in Women Who Have Previously Failed Attempts at Pregnancy With IVF

2018 ◽  
Vol 7 (3) ◽  
pp. 269-275
Author(s):  
Tasneem Mohamed ◽  
Yasmin Adam ◽  
Mohamed Iqbal Cassim
Keyword(s):  
Pregnancy Outcome ◽  
Endometrial Thickness ◽  
Univariate Analysis ◽  
Cross Sectional Study ◽  
Age Difference ◽  
Cross Sectional ◽  
Vitro Fertilization ◽  
Younger Women ◽  
The Mean

Objectives: The present study aimed to evaluate the effects of granulocyte colony-stimulating factor (G-CSF) as an adjunct to in vitro fertilization (IVF). In other words, it delved into the influence of G-CSF on the endometrium and the achievement of pregnancy. Materials and Methods: This retrospective cross-sectional study was conducted on a subgroup of women with two or more previously failed IVFs, who attended a fertility clinic in Johannesburg. These women underwent a procedure of transvaginal infusion of G-CSF in addition to their IVF protocol although endometrial thickness was not a criterion for G-CSF use. Results: The group included 49 women with a mean age of 38.9 years (SD ± 6.11). The mean number of previous IVFs was 3.1 (SD ± 1.76). The mean endometrial thickness pre-G-CSF and post-G-CSF was 7.53 mm (SD ± 2.69) and 9.11 mm (SD ± 2.12), respectively. The clinical pregnancy rate was 34.69%. Univariate analysis between the groups of women who achieved or failed to achieve pregnancy showed that the age difference between the two groups was statistically significant (P=0.0005). Further, G-CSF use was associated with increased pregnancy rates in younger women. Finally, although the mean endometrial thickness pre and post-G-CSF were not statistically significant between the two groups (P>0.05), the mean change in endometrial thickness was statistically significant in all women regardless of pregnancy outcome (P=0.0029). Conclusions: In general, G-CSF is considered as a useful adjunct for the treatment of women with recurrent failed IVFs and aged less than 38 years. Based on the findings, a statistically significant overall expansion of endometrial thickness was reported by using G-CSF. However, it failed to show any association between endometrial expansion and pregnancy outcome.

THE RELATIONSHIP BETWEEN GDF-9 AND BMP-15 SERUM AND FOLLICULAR FLUID AND THE QUALITY OF OOCYTES IN WOMEN WHO UNDERGO AN IVF CYCLE

2020 ◽  
pp. 22-27
Author(s):  
ZAINAL ARIFIN ◽  
KANADI SUMAPRADJA ◽  
UPIK ANGGRAENI ◽  
NAYLAH MUNA ◽  
BUDI WIWEKO
Keyword(s):  
Follicular Fluid ◽  
Statistical Correlation ◽  
Average Level ◽  
Vitro Fertilization ◽  
Fertilization Rates ◽  
Secreted Factors ◽  
The Mean ◽  
The Relationship

Objective: The quality of the embryo is greatly influenced by the quality of the oocytes; oocyte-secreted factors (OSFs), which include GDF-9 and BMP-15, play an important role in folliculogenesis. This study was to determine the relationship between GDF-9 and BMP-15 serum within the follicular fluid in order to predict the quality of oocytes in women undergoing In vitro Fertilization (IVF). Methods: We collected 30 samples of blood serum and 30 samples of follicular fluid on the day of ovum pickup (OPU), and examined GDF-9 and BMP-15 using ELISA kits. Analysis by Pearson and a partial-correlation was conducted to analyze the correlation between the concentration of GDF-9 and BMP-15 in serum and follicular fluid with general physiological parameters, such as maturation and fertilization rates. Results: The mean age of the subjects was 35,0(26,0-39,0) years. There was no statistical correlation between GDF-9 serum and follicular fluid (p=0.245); but there was a correlation between BMP-15 serum and follicular fluid (p=0.001). Average level of GDF-9 in the follicular fluid was 163,0 pg/ml (48,0-537), and average level in the serum was 260.33 pg/ml±121,82; average levels of BMP-15 in the follicular fluid was 58.30pg/ml±31,54 and average levels of BMP-15 in the serum was 74.20 pg/ml (1,0–610). Conclusion: There were no correlations between levels of GDF-9 serum-FF and BMP-15-FF, and maturation rates and fertilization rates. There was a negative correlation between BMP-15 serum and maturation rates.

scholarly journals Follicular fluid oxidative stress biomarkers and ART outcomes in PCOS women undergoing in vitro fertilization: A cross-sectional study

2021 ◽  
Author(s):  
Kaivalya Gongadashetti ◽  
Pankush Gupta ◽  
Rima Dada ◽  
Neena Mahotra
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cleavage Rate ◽  
Cross Sectional ◽  
Vitro Fertilization ◽  
Key Words Oxidative Stress

Background: Polycystic ovarian syndrome (PCOS) is the most common cause of anovulatory infertility. Oxidative stress (OS), which plays an important role in determining the developmental competence of an oocyte, may be involved in understanding infertility and poor outcomes cycles in PCOS women undergoing in vitro fertilization (IVF). Objective: To measure OS biomarkers in the follicular fluid of PCOS women undergoing IVF. Materials and Methods: In this cross-sectional study, 100 women with PCOS (n = 43) and tubal factor (n = 57) undergoing IVF, who were referred to a tertiary medical center between January 2016 and September 2017 were enrolled. OS markers like reactive oxygen species (ROS), total antioxidant capacity (TAC), and 8-Isoprostane (8-IP) were tested in the follicular fluid and various IVF outcomes in the form of oocytes retrieved, fertilized, cleavage rate, grading of embryos and pregnancy outcomes were compared between the two groups. Results: The results indicated that the levels of ROS, TAC, and 8-IP were higher in the PCOS group compared to the tubal group (p = 0.21, p = 0.95, and p < 0.05, respectively). Biomarkers based on the number of retrieved oocytes, cleavage rate, and grades of embryos did not differ significantly in the two groups. The median ROS, TAC, and 8-IP were not significantly different in the two groups in relation to the pregnancies, although the 8-IP levels were significantly raised in the PCOS women who had a miscarriage (p = 0.02). Conclusion: This study concluded the possible role of OS in PCOS women with increased higher level of 8-IP biomarker as a potential biomarker that needs further evaluation. Key words: Oxidative stress, ART, PCOS, Infertility, 8-IP.

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