scholarly journals Indigenous Phosphate Solubilizing Bacteria from Peat Soil for an Eco-friendly Biofertilizer in Oil Palm Plantation

KnE Energy ◽  
2015 ◽  
Vol 1 (1) ◽  
pp. 65 ◽  
Author(s):  
Elizabeth Caroline Situmorang ◽  
Andriessa Prameswara ◽  
Hana Christine Sinthya ◽  
Nurita Toruan-Mathius ◽  
Tony Liwang
Keyword(s):  
Renewable Energy ◽  
Soil Fertility ◽  
Oil Palm ◽  
Biochemical Characterization ◽  
Peat Soil ◽  
Essential Element ◽  
Soil Microbiology ◽  
Phosphate Solubilizing Bacteria ◽  
Medium Temperature ◽  
Phosphate Solubilizing

<p>Degradation in soil fertility is an enormous problem in agricultural intensification system. Availability in phosphate is one of among encountered problems. Peat soils have large areas and great potential for agriculture and plantations, but have many obstacles in their management. One of the issues is the low soil fertility and low availability of nutrients, especially phosphate which exists in many organic forms that are not available to plants. Phosphorous is an essential element after nitrogen, which plays an important role in plant growth and metabolism and the process of soil microbiology. Introduction of phosphate- solubilizing bacteria (PSB) as biofertilizer, is a renewable energy based which can increase the availability of phosphate to plants can overcome this problem and at the same time reducing the use of inorganic P fertilizer. Indigenous PSB, isolated from palm oil plantation could be used as a better and friendly bio-agent to improve soil fertility, specifically for phosphate availability. Eight PSB have been isolated from peat soil in oil palm platation. Morphological, biochemical characterization and molecular identifications showed that the 8 isolates belonged to Flavobacterium and Enterobacter genera, more precisely the species of Burkholderia sp and Novosphingobium nitrogenifigens. Characterization of isolates of the highest PSB activity consisted of the ability to dissolve phosphate in Pikovskaya solid medium, temperature resistance optimization and the ability to produce phosphatase enzyme. Results showed that isolate with the highest potentiality of dissolving phosphate formed 3.5 cm of clear zone diameter and the phosphatase activity at 9 days of incubation 5.992 units (μmol / ml / min). The peak of enzyme’s activity was reached at 6 days , which corresponded to 54.782 ppm of phosphorus solubilised. The lowest pH (3.2) was reached at 1st day of incubation. These isolates showed the ability to grow optimally at temperature range of 25 to 300C. Indigenous PSB could be used as biofertilizer agents which are eco friendly.</p><p><strong>Keywords</strong>: indigenous; biofertilizer; renewable energy, phosphate solubilizing bacteria;phosphatase; oil palm</p>

scholarly journals Potensi bakteri pelarut P dan Penambat N rhizosper kelapa sawit gambut saprik

Jurnal Agro ◽  
10.15575/5776 ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 14-23
Author(s):  
Ida Nur Istina ◽  
Happy Widiastuti ◽  
Benny Joy
Keyword(s):  
Oil Palm ◽  
Symbiotic Nitrogen Fixation ◽  
Soil Depth ◽  
Peat Soil ◽  
N Fixation ◽  
Phosphate Solubilizing Bacteria ◽  
Plate Method ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing ◽  
Biological Fertilizer

Pemanfaatan pupuk hayati sangat berpotensi untuk menurunkan input produksi pada budidaya kelapa sawit khususnya pupuk. Penelitian bertujuan untuk mendapatkan Bakteri Pelarut P dan Penambat N yang berpotensi sebagai bahan pupuk hayati dari rizosfer tanaman kelapa sawit. Penelitian dilakukan di lahan gambut saprik Kabupaten Pelalawan, Provinsi Riau dari bulan Juni sampai November 2014. Pengambilan sampel tanah dilakukan menggunakan bor gambut pada bagian rizosfer dengan kedalaman sampai 20 cm. Isolasi dan karakterisasi dengan metode pure plate menggunakan media selektif N Ashby untuk penambatan N dan Pikovskaya untuk pelarutan P, sedangkan analisis fiksasi N dan pelarutan P dilakukan menggunakan HPLC dan spektrofotometer di Laboratorium mikrobiologi PT. RPN. Hasil penelitian menunjukkan bahwa tanah gambut saprik berpotensi sebagai sumber isolat bakteri pelarut P dan penambat N potensial. Jumlah isolat bakteri pelarut P yang berhasil diperoleh adalah 11 isolat sedangkan jumlah bakteri penambat N non-simbiotik adalah 6 isolat. Isolat bakteri pelarut P potensial asal Sungai Ara dengan kemampuan melarutkan P 329,94 ppm; sedangkan bakteri penambat N non-simbiotik potensial adalah asal Kuala Panduk dengan kemampuan fiksasi N 0,0293 mmol l-1jam-1. ABSTRACTUtilization of biofertilizer is potential to decrease production inputs on oil palm cultivation, especially fertilizer expense. The research aimed to obtain Solubilizing P-Bacteria and Non-Symbiotic Fixing N bacteria which potential as biological fertilizer from oil palm rhyzosphere. The research was conducted at Pelalawan sapric peat soil from June to November 2014. Soil samples were taken by using peat drill into 20 cm soil depth, while isolation and characterization used pure plate method by using the selective media N Ashby for N fixation and Pikovskaya for P solubility. N fixation and P dissolution analyzed by using HPLC and spectrophotometer at PT. RPN microbiology laboratory. The results showed that sapric peat soil potentially utilize as microbial resource. The number of phosphate solubilizing bacteria isolates were 11 isolates, while the number of non-symbiotic nitrogen fixation bacteria inhibiting N Azotobacter sp. were 6 isolates. The potential isolate of P-solubilizing bacteria was Sungai Ara origin with the ability to dissolve P about 329.94 ppm; while the potential of non-symbiotic N-fixing bacteria was Kuala Panduk origin with N fixation ability 0.0293 mmoll-1h-1.

scholarly journals PENGARUH PUPUK HAYATI DAN DOSIS AZOTOBACTER SP. DALAM FITOREMEDIASI MENGGUNAKAN TANAMAN RAMI (BOEHMERIA NIVEA L. GAUD) PADA TANAH TERCEMAR HIDROKARBON MINYAK BUMI

2018 ◽  
Vol 22 (1) ◽  
pp. 13
Author(s):  
Gea Anggun Pratiwi ◽  
Diyan Herdiyantoro ◽  
Pudjawati Suryatmana
Keyword(s):  
Soil Fertility ◽  
Chlorophyll Content ◽  
Petroleum Hydrocarbon ◽  
Hydrocarbon Biodegradation ◽  
Phosphate Solubilizing Bacteria ◽  
Total Chlorophyll ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Total Chlorophyll Content ◽  
Phosphate Solubilizing

Phytoremediation in soil contaminated with petroleum hydrocarbon is a technology to remediate contaminant using plant as nutrient availability facilitator for degrading microbials in the rhizosphere. Phytoremediation can be promoted by biofertilizers containing nitrogen fixer, phosphate solubilizer, and mychorrizal for improving soil fertility, also Azotobacter sp. which produces biosurfactant. The purpose of this research was to determine the effects of biofertilizers and doses of Azotobacter sp. on enhancing of petroleum hydrocarbon biodegradation efficiency, nitrogen fixing bacteria and phosphate solubilizing bacteria population, and total chlorophyll content in ramie leaves. This research was carried out from September 2016 to April 2017 at Laboratory of Soil Biology, Laboratory of Soil Fertility and Plant Nutrition, also Greenhouse and Field Station Faculty of Agriculture Universitas Padjadjaran. This research used factorial randomized complete block design with two factors consisted of biofertilizers (control, nitrogen fixing bacteria, phosphate solubilizing bacteria, phosphate solubilizing fungi, mychorrizal, and biofertilizer consortium) and doses of Azotobacter sp. (control, 1%, and 2%). The results of experiment showed there was an interaction effect between biofertilizer of phosphate solubilizing fungi and 2% dose of Azotobacter sp. on the enhancement of total chlorophyll content in ramie leaves.

scholarly journals Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut

2018 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Ella Dewani Larasati ◽  
MG Isworo Rukmi ◽  
Endang Kusdiyantini ◽  
R Cinta Badia Ginting
Keyword(s):  
Growth And Development ◽  
Bacillus Pumilus ◽  
Peat Soil ◽  
Species Level ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Peat Soils ◽  
Biolog System ◽  
Phosphate Dissolution ◽  
Phosphate Solubilizing

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification

scholarly journals Growth and Yield of Lettuce (Lactuca sativa L.) by Giving Cow Manure and Empty Palm Oil Bunch Fertilizer on Peat Soil

Akta Agrosia ◽  
2020 ◽  
Vol 23 (2) ◽  
pp. 39-46
Author(s):  
Yedija Manullang ◽  
Reny Herawati ◽  
Merakati Handajaningsih ◽  
Dwi Wahyuni Ganefianti ◽  
Eka J.V. Haquarsum ◽  
...  
Keyword(s):  
Soil Fertility ◽  
Lactuca Sativa ◽  
Oil Palm ◽  
Peat Soil ◽  
Growth And Yield ◽  
Empty Fruit Bunch ◽  
Cow Manure ◽  
Lactuca Sativa L ◽  
Lettuce Growth ◽  
Four Levels

Lettuce (Lactuca sativa L.) is a horticultural plant that is beneficial for health. Efforts are being made to expand the plant area. One of them is by utilizing marginal land such as peatlands.  Peatlands in Indonesia are the most extensive soils in various tropical countries in the world that have low soil fertility and high acidity so that they are not optimal for lettuce growth. The provision of organic material can increase soil fertility by improving physical, chemical, and biological soil properties so that it can support lettuce growth. This study aims to determine the dose of cow manure and the dosage of oil palm empty fruit bunch fertilizer and the optimal dose interaction for growth and yield of lettuce. This research was conducted from June 2019 to August 2019 in Bengkulu City. This study uses a Completely Randomized Design consisting of two factors, which are repeated three times. The first factor is the dose of cow manure with three levels, namely: 0 tons ha-1, 15 tons ha-1, and 30 tons ha-1. The second factor is the dose of oil palm empty fruit bunch fertilizer, which consists of four levels, namely 0 tons ha-1, 5 tons ha-1, 10 tons ha-1, and 15 tons ha-1. The results showed that the dosage of cow manure 15 tons ha-1 and without the application of empty fruit bunches fertilizer gave the best results in leaf length of 18.674 cm. The best dose of cow manure for growth and yield of lettuce is 15 ha-1. The application of OPEFB fertilizer has not been shown to affect the growth and yield of lettuce.

scholarly journals Application of Empty Fruit Bunches Compost and Types of P Fertilizer on the Growth and Phosphorus Uptake in Oil Palm Seedlings

2020 ◽  
Vol 4 (2) ◽  
pp. 59
Author(s):  
Pauliz Budi Hastuti ◽  
Sri Manu Rohmiyati
Keyword(s):  
Oil Palm ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Phosphate Solubilizing Bacteria ◽  
Empty Fruit Bunches ◽  
P Fertilizer ◽  
Number Of Leaves ◽  
Phosphate Solubilizing ◽  
Growth Of Seedlings ◽  
Randomized Method

<p>This research aimed to evaluate the effect of empty fruit bunches (efb) compost, phosphate-solubilizing bacteria (PSB), lime, and types of P fertilizer on the growth and availability of Phosphorus and P uptake in the pre-nursery (PN) oil palm seedlings in latosols. The pot experiments were arranged in a Factorial Completely Randomized method. The first factor was the application of efb compost: without efb compost, efb compost, without efb compost + PSB, and without efb compost + lime. The second factor was the types of P fertilizer: SP-36, RP, guano, and NPKMg + Urea as a control. The results showed that the combination of without efb compost +lime and RP fertilizer produces the highest number of leaves. The influence of a single factor showed that the application of without efb, efb compost, without efb compost +PSB, without efb compost + lime, resulted in the same growth of seedlings, except on plant height and the length of the leaf. The various types of P fertilizer led to the same growth of seedlings except for stem diameter. The highest available P was obtained in the combination of without efb compost + PSB with SP-36 fertilizer, which was 631.1% compared to control (without efb compost and NPKMg, Urea) and the lowest in the combination of without efb compost + lime with (NPKMg, Urea). The treatment without efb compost + lime with SP-36 fertilizer increased P uptake of leaves by 55.6% and stem by 47.1% compared to control.</p>

scholarly journals Isolation and Identification of Elite Phosphate Solubilizing Bacteria from Soil under Paddy Cultivation

2014 ◽  
Vol 16 ◽  
pp. 62-68 ◽  
Author(s):  
A. Gandhi ◽  
G. Muralidharan ◽  
E. Sudhakar
Keyword(s):  
Bacterial Species ◽  
Essential Element ◽  
Broth Culture ◽  
Bacillus Species ◽  
Available Phosphorus ◽  
Growth Media ◽  
Screening Methods ◽  
Phosphate Solubilizing Bacteria ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

A considerable number of bacterial species are able to exert a beneficial effect upon plant growth. Mostly they are associated with the plant rhizosphere, so they are called as rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Several scientists have reported the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate. Detection and estimation of the phosphate solublization ability of microorganisms have been possible using plate screening methods. Phosphate solubilizers produce clearing zones around the microbial colonies in growth media. In the present investigation a total number of fifteen phosphate solubilizing bacterial colonies isolated from different paddy soils in Cuddalore district of Tamilnadu, India. The isolated PSB were identified and characterized for effective use in the field. All the PSB isolates were identified as Bacillus species and designated as P with serial number from 1 to 15. Among the fifteen isolates, the PSB isolate P6 showed highest amount of phosphate solubilization. The quantity of available phosphorus estimated in the P6 grown Sperber broth culture medium on 7th day was maximum of 321.7 μg/ml which was the highest value compared to other PSB isolates.

scholarly journals Phylogenetic Relationship of Phosphate Solubilizing Bacteria according to 16S rRNA Genes

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Mohammad Bagher Javadi Nobandegani ◽  
Halimi Mohd Saud ◽  
Wong Mui Yun
Keyword(s):  
16S Rrna ◽  
Oil Palm ◽  
Phosphorus Uptake ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Isolates ◽  
Serratia Plymuthica ◽  
Phosphate Solubilizing

Phosphate solubilizing bacteria (PSB) can convert insoluble form of phosphorous to an available form. Applications of PSB as inoculants increase the phosphorus uptake by plant in the field. In this study, isolation and precise identification of PSB were carried out in Malaysian (Serdang) oil palm field (University Putra Malaysia). Identification and phylogenetic analysis of 8 better isolates were carried out by 16S rRNA gene sequencing in which as a result five isolates belong to the Beta subdivision ofProteobacteria, one isolate was related to the Gama subdivision ofProteobacteria, and two isolates were related to theFirmicutes. Bacterial isolates of 6upmr, 2upmr, 19upmnr, 10upmr, and 24upmr were identified asAlcaligenes faecalis. Also, bacterial isolates of 20upmnr and 17upmnr were identified asBacillus cereusandVagococcus carniphilus, respectively, and bacterial isolates of 31upmr were identified asSerratia plymuthica. Molecular identification and characterization of oil palm strains as the specific phosphate solubilizer can reduce the time and cost of producing effective inoculate (biofertilizer) in an oil palm field.

scholarly journals Study of Root Exudate Organic Acids and Microbial Population in the Rhizosphere of Oil Palm Seedling

2017 ◽  
Vol 22 (1) ◽  
pp. 29-36
Author(s):  
. Anandyawati ◽  
Enok Sumarsih ◽  
Budi Nugroho ◽  
Rahayu Widyastuti
Keyword(s):  
Organic Acids ◽  
Root Exudates ◽  
Oil Palm ◽  
Root Exudate ◽  
Microbial Population ◽  
Phosphate Solubilizing Bacteria ◽  
Randomized Design ◽  
Increase Productivity ◽  
Positive Correlations ◽  
Phosphate Solubilizing

Mutual interaction between plants and microbes occured in the rhizosphere is expected to increase productivity of crops or soil fertility for agriculture. Plants excrete root exudates to attract microbes, and then microbes obtain habitat and food supply from plants and can fulfill the nutrient requirements through assisted enzymatic activity.  The objective of the research was to study the types and amounts of root exudate organic acids, microbial population, and the relationship between root exudate organic acids and microbial population in the rhizosphere of oil palm seedlings. The study was conducted in a greenhouse using a planting medium of sterile quartz sand. The study was conducted using two factorials completely randomized design with three replications. The first factor was oil palm seedling age (control / no oil palm seed, 1, 3, 6, 9 and 12 months-old of oil palm seedlings) and the second factor was the periods of seedling growth (45, 90, 135 and 180 days), so in total there were 72 experimental units. The result of High Pressure Liquid Chromatography (HPLC) analysis revealed that four kinds of organic acids were observed in the rhizosphere of oil palm seedlings, with the highest concentration were: acetic acid (1.66 ppm), citric acid (0.157 ppm), malic acid (2.061 ppm) and oxalic acid (0.675) ppm. The highest total population of microbes, fungi, Azotobacter, phosphate solubilizing bacteria (PSB) and phosphate solubilizing fungi (PSF) were 19.38 × 106 cfu g-1 soil, 3.28 × 104 cfu g-1 soil, 12.09 × 105 cfu g-1 soil, 8.39 × 104 cfu g-1 soil and 1.15 × 104 cfu g-1 soil, respectively. There are positive correlations between root exudate organic acids and total microbes, fungi, Azotobacter, PSB and PSF are.Keywords: microbes, organic acids, rhizosphere, root exudates

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