scholarly journals Expression of Cyclin D1 in Oral Squamous Cell Carcinoma

2021 ◽  
Author(s):  
Malak Abdulrahman Seid Ahmed ◽  
Nazik Omer ◽  
Ahmed M. Suliman ◽  
Mona Ellaithi
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cyclin D1 ◽  
Squamous Cell ◽  
Normal Cell ◽  
P Value ◽  
Female Ratio ◽  
Normal Cell Cycle

Background: Cyclin D1 expression regulates normal cell cycle. Its deregulation or overexpression may cause disruption in the normal cell cycle control and lead to cancer progression. In this study, we aimed to study the expression of cyclin D1 in oral squamous cell carcinoma (OSCC) and find its association with the different grades of oral tumors, if any.  Methods: This cross-sectional study included 40 formalin-fixed paraffin-embedded tissue blocks specimens of OSCC with variable grades. The expression of cyclin D1 was evaluated through immunohistochemical (IHC) staining. Results: There were 9 female and 31 male samples, with a male-to-female ratio of 3.4:1. The age ranged between 25 and 90 years with an average age of 65.5 years. Twenty-five (62.5%) samples were diagnosed as well-differentiated squamous cell carcinoma (WDSCC) and fifteen (37.5%) as poorly differentiated squamous cell carcinoma (PDSCC). No cases of moderately differentiated squamous carcinoma were included in the study. The expression of cyclin D1 was detected in the cases of WDSCC and a lesser expression was seen in the PDSCC with a P-value of 0.0003, OR 1581 and 95% CI (29.8239 to 83810.7113). Conclusion: Cyclin D1 is expressed in  OSCC and stronger expression was detected in WDSCC.

scholarly journals Elucidation of β-Catenin Cyclin D1 Pathway in Oral Squamous Cell Carcinoma

2018 ◽  
Vol 2 (2) ◽  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cyclin D1 ◽  
Squamous Cell ◽  
Cell Cycle Regulation ◽  
Mrna Level ◽  
Wnt Signaling Pathway ◽  
Cycle Regulation

Background: Oral cancer is sixth most common cancer in India with poor overall disease free survival. In last decade major changes in the cancer management has happened but no such advantage has been seen in the survival of oral cancer patients. One major reason for the poor survival of head and neck squamous cell carcinoma (HNSCC) is lack of good predictive and prognostic biomarkers. Different studies have shown that in cancer cells, cell-cycle regulatory protein expression is altered. Cyclin D1 is a key regulatory molecule in cell cycle regulation. Many of the molecular alterations that cause abnormal biologic behaviour of cancer cells are based on aberrations of cell cycle regulation. Studies have demonstrated that Cyclin D1, c-Myc and MMP7 were important target genes of WNT signaling pathway and overexpression of them was highly associated with accumulation of β-Catenin and mutational defects of the WNT signaling pathway in numerous tumor types. Aim: This study was planned to characterize the β-Catenin and Cyclin D1 transcript level expression pattern in oral squamous cell carcinoma (OSCC) samples. Materials and Methods: Expression patterns of β-Catenin and Cyclin D1 were studied in OSCC at the transcript and protein levels by using qRT-PCR and immunohistochemistry (IHC) respectively. χ2, t-tests and ANOVA were used for the statistical analyses. Results: β-Catenin and Cyclin D1 were significantly overexpressed in oral squamous cell carcinoma cases when compared to normal. Correlation regression analysis showed the expression of Cyclin D1 and β-Catenin at mRNA level were positively correlated. Further, in immunohistochemical analysis β-Catenin showed cytoplasmic staining rather than nuclear. Conclusion: It is concluded that β-Catenin and Cyclin D1 mRNA level analysis using Real-time PCR could serve as biomarkers in oral squamous cell carcinoma since their expression is consistently altered in majority of the oral squamous cell carcinoma samples.

scholarly journals Matrix metalloproteinase-9 (MMP-9) as prospective histopathological and molecular biomarker for oral squamous cell carcinoma

2018 ◽  
Vol 6 (8) ◽  
pp. 2801
Author(s):  
Bhagyalakshmi Atla ◽  
Sreevalli Prabhakula ◽  
Seeram Satish Kumar ◽  
Lakshmi Saraswathi Boni
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Treatment Strategies ◽  
Matrix Metalloproteinase 9 ◽  
P Value ◽  
Female Ratio ◽  
Metalloproteinase 9

Background: Despite the advance of therapeutic approaches, the morbidity and mortality of oral squamous cell carcinoma have not improved significantly. The invasion potential of oral squamous cell carcinoma is conferred by their ability to utilize Matrix metalloproteinase that are produced by the host’s stromal cells. In the present study an effort was made to analyse the role of clinical features and risk factors in the causation of oral squamous cell carcinoma and expression of Matrix metalloproteinase-9 (MMP-9) was studied in various grades of squamous cell carcinoma with its role in metastasis.Methods: The present study is an hospital based prospective study for a period of two years at the department of pathology from August 2014 to July 2016 consisting of 88 cases of oral squamous cell carcinoma. Tissue for H and E sections were fixed in 10% formalin and subjected to routine paraffin embedded processing and stained with hematoxylin and eosin.Results: Total number of cases of oral squamous cell carcinoma analyzed were 88, out of which 65 cases were subjected to MMP 9 expression by random selection on Immunohistochemistry. Majority of cases occurred in the age group of 41-50 years with male: female ratio of 1.4:1. In stage I and II the expression of MMP-9 is low in 37 cases (82.22%) and in stage III the expression is high in 14 cases (70%) with P value <0.05 (significant).Conclusions: Matrix metalloproteinases could act as a therapeutic target and could play an important role in effective treatment strategies of oral cancer.

scholarly journals Peri-neural Invasion in Oral Squamous Cell Carcinoma (OSCC): A Clinico-pathological Marker of OSCC Prognosis and Risk Assessment

2021 ◽  
pp. 1-9
Author(s):  
Priyankar Singh ◽  
Zeenat S. Imam ◽  
Sidhharth Singh ◽  
Setu Sinha ◽  
Varsha Singh ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
P Value ◽  
Age Group ◽  
Treatment Protocols ◽  
Female Ratio ◽  
Neural Invasion

Aims And Objectives: To assess the incidence of Peri-Neural invasion in oral squamous cell carcinoma (OSCC), and to notify its significance as potent risk factor in OSCC recurrence and metastasis. Study Design and Place of Study: A retrospective in vitro study was conducted at Indira Gandhi Institute of Medical Sciences, Patna, after approval of ethical committee Material and Methods: Clinical, histopathological and follow-up details of 86 cases of oral squamaous cell carcinoma were collected from medical records as secondary data from December 2019 till April 2021. Peri-Neural invasion was assessed in all patients and was associated with, lymph node involvement and with the different histologic grades of OSCC. Location, density and patterns of Peri-Neural invasion was checked along with extracapsular spread and survival. Chi-square test was used for analyzing statistical data. P value of < .05 was considered as significant Results: On statistical analysis it was found that, out of total 86 cases of OSCC, 59 were primary and 27 were recurrent OSCC. Male to female ratio was 3:1 and primary cases were more in age group of 35-44 years where as recurrent OSCC were more in age group of 55-64 years. Out of 59 primary cases of OSCC, Peri-Neural invasion was seen in 27 cases and out of 27 recurrent OSCC 18 had Peri-Neural invasion with a total of 45 cases with Peri-Neural invasion out of 86. Intratumor location was assessed in 22 cases and density of 1-2 nerves per section was seen. “Crescent” pattern was commonly occurring pattern in our study. Conclusion: Our study affirmed that the incidence of Peri-Neural invasion is high in OSCC. It is present in both primary and recurrent tumors, irrespective of tumor’s grading. PNI must be assessed in all cases of OSCC as it gives markable impression on recurrence, metastasis and prognosis, and modulates treatment protocols.

scholarly journals Cyclin D1 Expression and Its Correlation with Histopathological Differentiation in Oral Squamous Cell Carcinoma

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Swati Saawarn ◽  
Madhusudan Astekar ◽  
Nisheeth Saawarn ◽  
Nidhi Dhakar ◽  
Shitalkumar Gomateshwar Sagari
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cyclin D1 ◽  
Squamous Cell ◽  
Cell Cycle Control ◽  
Clinical Staging ◽  
Formalin Fixed Paraffin Embedded ◽  
Well Differentiated

Background. Cyclin D1 regulates the G1 to S transition of cell cycle. Its deregulation or overexpression may lead to disturbance in the normal cell cycle control and tumour formation. Overexpression of cyclin D1 has been reported in various tumors of diverse histogenesis. This case control retrospective study was carried out to study the immunohistochemical reactivity and expression of cyclin D1 and its association with site, clinical staging, and histopathological differentiation of oral squamous cell carcinoma (OSCC).Methods. Forty formalin-fixed paraffin-embedded tissue blocks of biopsy specimens of oral squamous cell carcinoma were immunohistochemically evaluated for expression of cyclin D1.Results. Cyclin D1 expression was seen in 45% cases of OSCC. It did not correlate with site and clinical staging. Highest expression was seen in well-differentiated, followed by moderately differentiated, and poorly differentiated squamous cell carcinomas, with a statistically significant correlation.Conclusion. Cyclin D1 expression significantly increases with increase in differentiation.

Immunohistochemical Expression of TGF-β3 in Oral Squamous Cell Carcinoma

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Asmaa Ali Hussein
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Transforming Growth Factor ◽  
Tumor Grade ◽  
Tumor Stage ◽  
High Rate ◽  
Female Ratio ◽  
Positive Expression

Squamous cell carcinoma characterized by poor prognosis due to aggressive tumor growth and dissemination high rate of tumor cell . age ranged of patient case included in the study 40-62 years and mean age 55±99. The sex distribution male/female ratio 1:1. Male case 15 and female 15 of the present study The results of clinical forums showed in the current study was endophytic 10(33.3%) in the same time Exophytic were presented in 20 cases (76.7%). Regarding distribution of the tumors site, the preponderance of them 19 cases 73.3% were located alveolar mucosa, followed by in the tongue 11 cases(36.7%) Tumor stage was analyzed and recorded in Oral squamous cell carcinoma included cases, the preponderance of them were Stage II 11 cases 36.7% followed by stage III 10 cases 33.3% , 9 cases 30.0% were stage I. While Concerning tumor grade, majority of them 15 cases 50% had grade II moderately differentiated SCC, while 11 cases 36.7% had grade III poorly differentiated SCC and 4 cases 13.3% had grade I well differentiated SCC Positive TGF-β3 immunostaining was detected as cell with staining brown color, all tissues sections included show Positive expression based on IHC teqnique. Positive Transforming Growth Factor TGF-β3 Immuno staining was found in all case results and display that 4 samples with percentage 13.3% expressed strong positive 87.67 ± 1.45 expression , 11cases 36.7% showed 51.33 ±0.88 positive expression moderate at the same time 15 samples 50.0% showed positive weak expression.

Cyclosporine A Suppresses the Malignant Progression of Oral Squamous Cell Carcinoma in vitro

2019 ◽  
Vol 19 (2) ◽  
pp. 248-255 ◽  
Author(s):  
Ling Gao ◽  
Jianwei Dong ◽  
Nanyang Zhang ◽  
Zhanxian Le ◽  
Wenhao Ren ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cyclosporine A ◽  
Migration And Invasion ◽  
Signal Molecules ◽  
Cox 2

Background:The Oral Squamous Cell Carcinoma (OSCC) is one of the most frequent cancer types. Failure of treatment of OSCC is potentially lethal because of local recurrence, regional lymph node metastasis, and distant metastasis. Chemotherapy plays a vital role through suppression of tumorigenesis. Cyclosporine A (CsA), an immunosuppressant drug, has been efficiently used in allograft organ transplant recipients to prevent rejection, and also has been used in a subset of patients with autoimmunity related disorders. The present study aims to investigate novel and effective chemotherapeutic drugs to overcome drug-resistance in the treatment of OSCC.Methods:Cells were incubated in the standard way. Cell viability was assayed using the MTT assay. Cell proliferation was determined using colony formation assay. The cell cycle assay was performed using flow cytometry. Apoptosis was assessed using fluorescence-activated cell sorting after stained by the Annexin V-fluorescein isothiocyanate (FITC). Cell migration and invasion were analyzed using wound healing assay and tranwell. The effect of COX-2, c-Myc, MMP-9, MMP-2, and NFATc1 protein expression was determined using Western blot analysis while NFATc1 mRNA expression was determined by RT-PCR.Results:In vitro studies indicated that CsA inhibited partial OSCC growth by inducing cell cycle arrest, apoptosis, and the migration and invasion of OSCC cells. We also demonstrated that CsA could inhibit the expression of NFATc1 and its downstream genes COX-2, c-Myc, MMP-9, and MMP-2 in OSCC cells. Furthermore, we analyzed the expression of NFATc1 in head and neck cancer through the Oncomine database. The data was consistent with the experimental findings.Conclusion:The present study initially demonstrated that CsA could inhibit the progression of OSCC cells and can mediate the signal molecules of NFATc1 signaling pathway, which has strong relationship with cancer development. That explains us CsA has potential to explore the possibilities as a novel chemotherapeutic drug for the treatment of OSCC.

scholarly journals Rural profile of oral squamous cell carcinoma (OSCC) survival patients attending in tertiary level hospital in Bogura: a hospital based retrospective observational study.

2020 ◽  
Vol 10 (1) ◽  
pp. 3-5
Author(s):  
Parometa Barma ◽  
Ibrahim Khalil ◽  
Tanzima Yeasmin
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Malignant Neoplasm ◽  
P Value ◽  
Oral Epithelium ◽  
Tertiary Level ◽  
Total Cancer ◽  
Grade Ii

Developing country like Bangladesh among 90% of oral malignant neoplasm are arising from squamous layer of oral epithelium which are third leading of this country with severe disfiguration, functiolaesa, psychological impairments and socio-economic hardship. In this study we found the prevalence of OSCC was 6.5% among total cancer patients in the two tertiary level hospitals of Bogura. Male (70%) are affected more than in females (30%). In response to male and female parameter, sex distribution ratio was 2.3:1. The prevalent average age was 55.40 years. About 80% of ulcer site was buccal mucosa then 10 % were lip mucosa. About 80% people were habituated by betel nut, leaf with tobacco chewers. Moreover 46.7% of them were maltreated by quack doctors before. Majority of the patient was in Grade II (56.7%). Correlation between variable in respect of age and cancer grading was explained. According to these study OSCC patients in north bangle region like Bogura was quite high on ( significant p-value ≤ 0.05). Description of oral squamous cell carcinoma on the basis of demographic and clinical profile was the major aim . Most of the cases report with intermediate grade of the disease which often leads to decrease the chance of survival of a patient. So new strategies should be considered to overcome the present situation must be undertaken by oral health programs for the early diagnosis and prevention, build up awareness and management and follow up of oral cancer. Update Dent. Coll. j: 2020; 10 (1): 3-5

scholarly journals p53 immunohistochemical staining patterns in oral squamous cell carcinoma

2016 ◽  
Vol 6 (12) ◽  
pp. 1013-1017
Author(s):  
G Dundy ◽  
H Kumar ◽  
A Singh ◽  
A Chandarakant
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
P53 Protein ◽  
P Value ◽  
P53 Expression ◽  
Normal Oral Mucosa ◽  
Significant Difference

Background: Mutation of p53 gene is one of the most common events in oral carcinogenesis. Accumulation of p53 protein has also been detected in premalignant lesions.Materials and Methods:  This study included 40 biopsy samples, which were received in department of pathology, Sarojini Naidu Medical College, Agra, to ascertain p53 expression by immunohistochemically, in patients with oral squamous cell carcinomas and to correlate its expression with histological grade, different sites in oral cavity and tobacco intake/smoking habits.Results: Out of 40 biopsies of oral mucosa, 03 showed normal oral mucosa and 37 were diagnosed as squamous cell carcinoma (SCC), most patients were in 5th and 6th decade and majority (86.5%) of oral SCC were males with buccal mucosa being the most common site. There was a statistically significant difference in p53 expression between oral SCC and normal oral mucosa (p value <0.05). Of total 37 cases, 12 cases were well differentiated type, 16 moderately differentiated and 09 of poorly differentiated type of SCC. In each category, about two thirds were positive for p53 staining. Out of total 37 cases of oral SCC, 64.9% were positive and 35.1% were negative for p53 expression, 34 cases had positive history of tobacco intake/smoking habits, of which 23 cases were positive while 11 cases were negative for p53 staining.Conclusion: Abnormal p53 protein was detected in 64.9% of oral squamous cell carcinoma, but not in normal oral mucosa. p53 expression was associated with malignant transformation of oral mucosa. 

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