scholarly journals Identification of the chemical constituents of aqueous and alcoholic Glycyrrhiza glabra root extracts and its comparative apoptotic study against human hepatocarcinoma cell line

2019 ◽  
Author(s):  
Nima Rezaiyan Gharagozlu ◽  
Fahimeh Baghbani-Arani ◽  
Seyed Ataollah Sadat Shandiz
Keyword(s):  
Cell Line ◽  
Aqueous Extract ◽  
Hepg2 Cells ◽  
Nuclear Dna ◽  
Chemical Constituents ◽  
Trimethylsilyl Ether ◽  
Ethanolic Extract ◽  
Glycyrrhiza Glabra ◽  
Root Extracts ◽  
Carbon Dioxide Co2

Introdution: Hepatocellular carcinoma (HCC) is one of the most common cancers. It is the third leading cause of cancer related death. This study aimed to evaluate the phytochemical composition of ethanolic and aqueous Glycyrrhiza glabra root extracts its apoptotic effects against human hepatocarcinoma HepG2 cell line. Methods: In the current experimental study, the phyochemical composition of aqueous and ethanolic Glycyrrhiza glabra extract was evaluated by GC/MS method. The HepG2 cells were grown in RPMI1640 supplemented with 10 % (v/v) FBS at 37°C with 5 %carbon dioxide (CO2) atmosphere. The cytotoxicity activities of extracts were measured using MTT after 24 hours. To evaluate the ability of extracts to trigger apoptosis in HepG2 cells, the Hosest8778 staining method was carried out. Results: The phytochemical data of aqueous extract revealed 18 compounds, including Dodecane (13.34%) and 2',6'-Dihydroxyacetophenone bis (trimethylsilyl) ether (12.48%) as the major constitutes. The chemical composition of ethanolic extract showed 17 compounds, including Pentadecane (7.78%), 2,6,11,15-Tetramethylhexadecane (4.67%) as the prominent compounds. The MTT results revealed that the extract-treated cells had a dose-related toxicity effects. The results of Hoechst 33258 staining showed that the nuclear DNA fragmentation was increased in the ethanolic extract treatment compared to aqueous extract. Conclusion: The ethanolic extract of Glycyrrhiza glabra possesses more apoptotic effect on cancer cells and further researches are needed for investigating its potential mechanism.

scholarly journals ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF MORINGA OLEIFERA

2016 ◽  
Vol 8 (4) ◽  
pp. 54 ◽  
Author(s):  
Burli Sanganna ◽  
Havagiray R. Chitme ◽  
Khanvilkar Vrunda ◽  
Mohsin J. Jamadar
Keyword(s):  
Antioxidant Activity ◽  
Growth Inhibition ◽  
Cell Line ◽  
Aqueous Extract ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Colon Cell ◽  
Colon Cell Line ◽  
Phytochemical Constituents ◽  
Ht 29

Objective: The aim of the study was to investigate the ethanolic and aqueous extract of leaves of Moringa oleifera for phytochemical constituents, antiproliferative and antioxidant activity.Methods: The ethanolic extract of leaves of Moringa oleifera, belonging to the family Moringaceae was prepared by using soxhlet apparatus and aqueous extract was prepared by using maceration process. The extract was evaluated for its phytochemical constituents. The antiproliferative effects of both extracts were checked by using MTT ([3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide]) assay on HT-29 colon cell line and the antioxidant activity were checked by using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. In antiproliferative and antioxidant activity the 5-FU (5-fluro uracil) and Ascorbic acid used as a standard drug for present results conclusion respectively.Results: The results obtained in MTT assay shown that ethanolic extract of Moringa oleifera had a more potent antiproliferative effect (growth inhibition of 62.25% at 100 μg/ml) on HT-29 colon cell line as compared to aqueous extract (% growth inhibition of 27.86 at 100 μg/ml) of Moringa oleifera. The ethanolic extract of Moringa oleifera shown more potent antioxidant activity (% inhibition of ethanolic 75.57 at 100 μg/ml) than aqueous extract (38.16 at 100 μg/ml) of Moringa oleifera. The activity shown by the extract is concentration dependent.Conclusion: In the present study we have investigated that the effect of ethanolic and aqueous leaves extracts of Moringa oleifera possess antiproliferative and antioxidant properties.

scholarly journals In-vitro Anti-inflammatory activity of Liquorice (Glycyrrhiza glabra) using Aqueous Extract

2020 ◽  
Vol 11 (1) ◽  
pp. 657-662
Author(s):  
Vasanth M P ◽  
Purushotham KG ◽  
Sathish M ◽  
Vimal Raj D ◽  
Venkatesh M
Keyword(s):  
Medicinal Plant ◽  
Cell Line ◽  
Aqueous Extract ◽  
Cytotoxicity Assay ◽  
Glycyrrhiza Glabra ◽  
Peroxide Radical ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Anti Inflammation

The G.glabra is otherwise called liquorice is a medicinal plant is used for various diseases like cold, cough, hypokalemia and muscle weakness, etc. The liquorice family belongs to the Fabaceae family of the G.glabra. Hence this study tells about the anti-inflammatory and antioxidants.  The quantitative study of phytochemical analysis, antioxidant and Anti-inflammatory, cytotoxicity assay using a response from the root extract of G.glabra  The results are showed above preliminary activity phytochemicals were present Alkaloids, Flavonoids, Coumarin, Saponins, Terpenoids, Steroids, Cardiac Glycosides. The antioxidant activity of aqueous extract of G. glabra were evaluated with the 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide radical (NO), superoxide radical (SO), hydrogen peroxide radical (H2O2), and hydroxyl radical (HO) scavenging activity. This study shows about the activity of Glycyrrhiza glabra herbal medicinal plant aqueous extract using antioxidant assays, Anti-inflammatory assays.  The in vitro MTT 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Cytotoxicity assay were studied GG(G.glabra)  extract using the macrophages  Raw 246.7 Cell line. The cell line assay were studied in two different activity. One is anti-inflammation studies, and another is cytotoxicty of GG aqueous extract. The anti-inflammation IC50 value is 143.65, and GG extract dry sample were against the IC50 value is 326.27. From above, the results were a potential activity of GG extract R-value 0f 0.991.

scholarly journals Chemical Constituents and Their Activities From the Twigs of Euscaphis konishii Hayata

2020 ◽  
Vol 15 (7) ◽  
pp. 1934578X2093493
Author(s):  
Jingxin Chen ◽  
Lin Ni ◽  
Yao Zhang ◽  
Yingsa Zhu ◽  
Wei Huang ◽  
...  
Keyword(s):  
Cell Line ◽  
Ellagic Acid ◽  
Betulinic Acid ◽  
Hepg2 Cells ◽  
Chemical Constituents ◽  
Hepatocarcinoma Cell ◽  
Human Hepatocarcinoma Cell Line ◽  
First Time ◽  
Hepatocarcinoma Cell Line ◽  
Tormentic Acid

A new ellagic acid derivative 3,3′-di- O-methylellagic acid 4 ′-α-l-arabinopyranoside (1), with 9 known compounds identified as 3,3′-di- O-methylellagic acid (2), 3,3′-di- O-methylellagic acid 4′-α-d-arabinofuranoside (3), 3,3′-di- O-methylellagic acid 4′ -β-d-glucopyranoside (4), 3,3′-di- O-methylellagic acid 4 ′-β-d-xylopyranoglucoside (5), 3,3′,4-tri- O-methylellagic acid 4′-β-d-glucopyranoside (6), tormentic acid (7), ursolic acid (8), euscaphic acid (9), and betulinic acid (10), was isolated from the twigs of Euscaphis konishii Hayata. Compounds 1, 3, and 5 to 7 were isolated from this plant for the first time, and compounds 1 and 5 were obtained from the plant genus for the first time. The structure of the new compound was confirmed by HRESIMS, NMR, and compared with data from the literature . The cytotoxicities of 10 isolated compounds were tested, with compounds 1 to 6 showing moderately inhibited activity against the Human Hepatocarcinoma cell line (HepG2 cells) with an IC50 value ranging from 69.7 to 181.8 μM.

scholarly journals Licorice (Glycyrrhiza glabra (Linnaeus, 1753)) roots aqueous extract and some additives against Bactrocera zonata (Saunders, 1841) (Diptera: Tephritidae)

2021 ◽  
Vol 90 (2 - Ahead of print) ◽  
pp. 70-85
Author(s):  
Mervat Abdel-Moneauim Mostafa El-Genaidy ◽  
Mohamed Abd El-Aziz Mohamed Hindy ◽  
Nehad Abdel-Hameed Soliman
Keyword(s):  
Aqueous Extract ◽  
Sandy Soil ◽  
Clay Soil ◽  
Mineral Oil ◽  
Glycyrrhiza Glabra ◽  
Clay Soils ◽  
Insecticidal Effect ◽  
Bactrocera Zonata ◽  
Light Mineral ◽  
Petroleum Oil

Peach fruit fly, Bactrocera zonata (Saunders, 1841) is a destructive polyphagous pest threatening the horticultural production in Egypt. Licorice, Glycyrrhiza glabra (Linnaeus, 1753) is a plant growing in Egypt and many other countries and famous for saponins groups that have insecticidal effect against broad spectrum of insect pests. In the present study, the insecticidal effect of licorice roots aqueous extract (LRAE), petroleum oil, KZ light mineral oil 96% (EC), water and an emulsion (1/4 L LRAE + ¼ L petroleum oil + ½ L KZ light oil 96% (EC)) treatments in a ratio 1 L: 29 L water were used in Matabi® sprayer of 30 L capacity against B. zonata pupae in sandy and clay soils. In sandy and clay soils LRAE reduced B. zonata population by 74.44% and 87.55% while petroleum oil, KZ light mineral oil 96% (EC) prevented flies emergence (100% reduction). Water treatment suppressed B. zonata population by 78.61% in sandy soil but caused 100% population reduction in clay soil. The emulsion reduced B. zonata population by 96.94% in sandy soil and 100% in clay soil. The best method for application of the emulsion was to spray as one target spray technique for eight seconds that was sufficient to obtain suitable coverage on soil with spray speed 1.2 km / hour. The persistence of the emulsion that highly reduced B. zonata larval populations was 3.5 and 4.5 days in sandy and clay soils, respectively. The flies emerged from B. zonata pupae treated with the emulsion neither feed nor move naturally. The histological studies showed that these flies suffered changes in the eyes, labellum, muscles and midgut tissues that were different from the emerged control treatment flies.

scholarly journals In- vitro Hepatoprotective Action of the Crude Extracts of Luffa amara (Whole Fruit) and Rheum emodi (Rhizomes) in CCl4 Intoxicated Rat Hepatocytes

2021 ◽  
pp. 396-404
Author(s):  
Muna Abid ◽  
Zakia Abid ◽  
B. Syed Asad ◽  
Mohammed Ibrahim
Keyword(s):  
Petroleum Ether ◽  
Aqueous Extract ◽  
Protective Action ◽  
Elevated Serum ◽  
Ethanolic Extract ◽  
Rat Hepatocytes ◽  
Ether Extract ◽  
Isolated Rat Hepatocytes ◽  
Primary Rat Hepatocytes

Aim: The objective of this in-vitro study involves evaluating the protective action of the extracts of L. amara (LA) (whole fruits including seeds) and R. emodi (RE) (rhizomes) at various concentrations on isolated primary rat hepatocytes. Methods: The pulverised dried whole fruits of L. amara (LA) and rhizomes of R.emodi (RE) were extracted successively with petroleum ether (PE), ethanol (EE) and distilled water (AE) and vacuum dried. These extracts of LA petroleum ether (PE), ethanolic (EE) and aqueous (AE) extracts and RE obtained were subjected to in vitro studies at doses of 25, 75, 100, and 150 µg/ml and silymarin (250 µg/ml) in CCl4 (1%) intoxicated primary hepatocytes monolayer cultures the hepatoprotective action of all the extracts of both plants at different doses was carried out using isolated rat hepatocytes which were subjected to CCl4 intoxication followed by estimating/ measuring the changes in serum biochemical markers – SGPT, SGOT, ALP, Total proteins (TP), total bilirubin (TB), albumin (ALB) and triglycerides (TGL). Results: Hepatoprotective activity against CCl4 was demonstrated in the rat primary monolayer hepatocyte culture using MMT assay with the ethanolic extracts of both plants showing more hepatocyte protective action compared to the aqueous and petroleum ether extracts by reducing the elevated serum marker levels. Alcoholic and aqueous extracts were found to express more protective action towards CCl4 intoxicated isolated primary rat hepatocytes in a dose dependant manner. Conclusion: Based on the result, it is suggested that the extract with the most hepatocyte protective action at a dose of 150µg is LA ethanolic extract (viability=88.24%), followed by LA aqueous extract (viability=84.31%), RE ethanolic extract (viability=88.24%) and RE aqueous extract (viability=88.24%) - which are comparable to the reference silymarin with viability at 92.15%. the petroleum ether extracts of both plants showed least hepatic cell viability with LA pet ether extract at 49.02% and RE pet ether extract at 47.85%

scholarly journals Evaluation of anticoagulant activity of aqueous extract of Cestrum nocturnum

2017 ◽  
Vol 8 (4) ◽  
pp. 525
Author(s):  
Chandra Kishore Tyagi ◽  
Deenanath Jhade ◽  
Sunil Kumar Shah
Keyword(s):  
Aqueous Extract ◽  
Anticoagulant Activity ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Thrombin Time ◽  
Clotting Time ◽  
Standard Procedures ◽  
Prolonged Aptt ◽  
Pharmacologically Active

<p>The study evaluated anticoagulant properties of the aqueous extract of <em>Cestrum nocturnum</em> using aPTT-Activated Partial Thromboplastin Time, PT- Prothrombin Time &amp; TT-Thrombin Time as standard procedures.</p><p>For <em>in vitro</em> coagulation assays, aqueous extract of plant prolonged APTT, TT, and PT clotting times in a dose-dependent manner (Table 7). It prolonged APTT clotting time from 45 ± 2 (2mg/mL) to 82.2 ± 2.63s (10mg/mL), PT clotting time from 20.4 ± 1.49 (2mg/mL) to 31.4 ± 2.15s (10mg/mL), and TT clotting time from 9.2 ± 1.16 (2mg/mL) to 17.4 ± 1.01s (10mg/mL) at the concentration of 2 to 10mg/mL. Heparin prolonged APTT and PT clotting times more than 111.8s and 40.8s, respectively, at a concentration of 1 IU/mL. Heparin prolonged TT clotting times more than 20.6s at a concentration of 1 IU/mL.</p><p>The phytochemical screening of the plant confirm the presence of saponin in the water and ethanolic extract, Alkaloid in all the extract except hexane extract, tannin in water, ethanol and methanol extract, amino acid in water and ethanolic extract, carbohydrate in water and methanolic extract and triterpenoids and glycoside were absent in all the extracts. The results demonstrated that the aqueous extract of <em>Cestrum nocturnum</em> possesses pharmacologically active anticoagulant principles that could be isolated and evaluated for clinical or physiological purposes.</p>

scholarly journals Phytochemistry and GC-MS screening and biocidal potentiality of clove (Syzygium aromaticum L.) pods against mosquitos’ larvae

2021 ◽  
Vol 3 (2) ◽  
pp. 083-089
Author(s):  
Dalia Mustafa M. Elbashir ◽  
Mutaman AA Kehail ◽  
Yasir Mohamed Abdelrahim ◽  
Abdelmonem Eltiyab H Ali
Keyword(s):  
Aqueous Extract ◽  
Mosquito Control ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Control Agent ◽  
Syzygium Aromaticum ◽  
Mosquito Nets ◽  
Economic Trends ◽  
Ethanol Extracts ◽  
Phytochemical Components

Many measures have been used for mosquito control, including the elimination of breeding places, exclusion via window screens and mosquito nets in addition to natural products including clove (Syzygium aromaticum). This study was run at University of Gezira, Sudan, to run phytochemical and GC-MS screening for clove pods before used it as mosquito control agent. The standard methods, materials and devices were used to screen the phytochemical components and the chemical constituents (GC-MS). The WHO protocol for testing the susceptibility of mosquito’s larvae to insecticides was followed in bioassay. The aqueous and the ethanol extracts from clove pods were prepared and used against Anopheles, Culex and Aedes larvae. The results showed that, Aedes mosquito was relatively more susceptible (LC50= 498 mg/L) to clove aqueous extract than Anopheles (LC50= 561 mg/L) and Culex (LC50= 615 mg/L), and similar findings were observed for clove pods ethanol extract, which is relatively more potent than the aqueous extract. The biocidal activity can be attributed to the presence of the detected saponins, flavonoids, tannins and alkaloids. The GC-MS for the ethanol extract showed that, the principal compounds were Eugenol (81%) and caryophyllene (4.65%). Further studies should be run to improve knowledge about how to use this natural product in more economic trends.

scholarly journals Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Faramarz Zakavi ◽  
Leila Golpasand Hagh ◽  
Arash Daraeighadikolaei ◽  
Ahmad Farajzadeh Sheikh ◽  
Arsham Daraeighadikolaei ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Antibacterial Effect ◽  
Juglans Regia ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Oral Bacteria ◽  
Disk Diffusion ◽  
Resistant Bacteria ◽  
Aqueous Extracts

Background. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria,Streptococcus mutans,Streptococcus salivarius,Streptococcus sanguis, andStaphylococcus aureus.Methods. Aqueous and ethanol extracts of Juglans regia bark were prepared by using disk diffusion technique and Minimal Inhibitory Concentration (MIC) methods. Tetracycline 30 μg and Erythromycin 15 μg were used as positive control and water as negative control in disk diffusion and MIC methods. Data were analyzed by ANOVA test.Results. The results showed thatS. sanguisandS. mutanswere the most sensitive and the most resistant bacteria against ethanolic and aqueous extracts, respectively. Ethanolic extract had significant antibacterial effect against all tested bacteria. Aqueous extract did not show antibacterial effect onS. mutans, in contrast to ethanolic extract. Aqueous extract had significantly antibacterial effect againstStaphylococcus aureus,S. salivarius, andS. sanguiscompared to control (P<0.0001), but it did not show effect onS. mutanswhen compared with Erythromycin. According to the obtained MIC values, ethanol extract of Juglans regia bark had the lowest rate.Conclusion. The results may provide the basis for using natural antimicrobial substance for oral hygiene prophylaxis purposes.

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