EFEKTIVITAS KELAMBU BERINSEKTISIDA DALAM PENGENDALIAN VEKTOR MALARIA DI INDONESIA

Malaria is still a health problem in several parts of Indonesia. National malaria elimination is targeted at 2030. One of the programs carried out by the government in malaria control to achieve elimination is the mass distribution and use of insecticide-treated mosquito nets. The use of insecticide-treated mosquito nets is one of the efforts to reduce malaria cases through vector control. Insecticide mosquito nets are recommended as a strategic step to break the chain of malaria transmission because the insecticide content in the treated fibers can kill mosquitoes. The data used in this paper is data on the percentage of mosquito mortality from the efficacy test of insecticide-treated mosquito nets to see the killing power of mosquito nets which is then used to assess the effectiveness of mosquito nets in vector control. The data were obtained from scientific studies of articles published in scientific journals. From the data collected, it is known that some insecticide-treated mosquito nets used by the community are effective in vector control, but some are no longer effective. The best period for using mosquito nets is less than 6 months by paying attention to the proper washing method so that insecticide-treated mosquito nets are still effective.

Deteksi Gen Kdr pada Nyamuk Anopheles di Kabupaten Maluku Tenggara Barat

Malaria is still a health problem in Indonesia, particularly in Eastern part of Indonesia. The use of LLIN insecticide bed nets is one of the efforts to reduce the malaria morbidity rate by protecting human from malaria vector bites. The Anopheles flavirostris, Anopheles barbirostris, and Anopheles subpictus mosquitoes are three of the species reported as malaria vectors in West-Southeast Maluku Regency. The aim of this research was to detect the kdr gene in An. flavirostris, An. barbirostris, and An.subpictus mosquitoes collected from Alusi Kelaan village, West-Southeast Maluku Regency. The research was conducted at the Papua Biomedical Research and Development Center, in June 2016. A total of six An. flavirostris, 42 An. barbirostris, and 24 An. subpictus were pooled separately for genomic DNA extraction. The sample used was the An. flavirostris, An. barbirostris, and An. subpictus that survived after the impregnated paper test. The kdr gene detection was carried out using quantitative PCR (qPCR) focused on points V1010 and L1014. The results showed that there were no kdr mutant strains in the An. flavirostris, An. barbirostris, and An. subpictus. These results indicated that the sensitivity of pyrethroid insecticides contained in LLIN mosquito nets to An. flavirostris, An. barbirostris, and An. subpictus mosquitoes was not decreased in West Southeast Maluku Regency.

Kajian Entomologi dalam Mendukung Pengendalian Malaria melalui Program Flying Health Care (FHC) di Kabupaten Yahukimo, Provinsi Papua

Flying Health Care is one of the programs of the Ministry of Health to accelerate the elimination of malaria in an integrated manner, especially in the Yahukimo Regency. The purpose of this activity is to describe the role of entomological aspects in malaria control efforts. The data includes cases of malaria, mosquitoes and Anopheles larvae, breeding place, and vector control efforts. Data was collected by observation, survey, and secondary data analysis. The results of the study show that malaria cases rank first out of 10 diseases in health facilities. Malaria cases were more common occurred at the age of 10-19 years, male and near to health facilities. Breeding places in the form of swamps and puddles in the environment around patients with water depths ranging from 5-30 cm. There is no entomological data at the district level or at Puskesmas Aplim. Ongoing vector control efforts are in the form of distributing insecticide-treated mosquito nets. Insecticide mosquito nets have not been fully distributed to the community, this is constrained by the existing infrastructure in health care facilities. Vector control efforts should be supported by the results of entomological surveys and it is important to do socialization on how to use and maintain mosquito nets at the time of mosquito net distribution.

Incidence and consequences of damage to insecticide-treated mosquito nets in Kenya

Background Efforts to improve the impact of long-lasting insecticidal nets (LLINs) should be informed by understanding of the causes of decay in effect. Holes in LLINs have been estimated to account for 7–11% of loss in effect on vectorial capacity for Plasmodium falciparum malaria in an analysis of repeated cross-sectional surveys of LLINs in Kenya. This does not account for the effect of holes as a cause of net attrition or non-use, which cannot be measured using only cross-sectional data. There is a need for estimates of how much these indirect effects of physical damage on use and attrition contribute to decay in effectiveness of LLINs. Methods Use, physical integrity, and survival were assessed in a cohort of 4514 LLINs followed for up to 4 years in Kenya. Flow diagrams were used to illustrate how the status of nets, in terms of categories of use, physical integrity, and attrition, changed between surveys carried out at 6-month intervals. A compartment model defined in terms of ordinary differential equations (ODEs) was used to estimate the transition rates between the categories. Effects of physical damage to LLINs on use and attrition were quantified by simulating counterfactuals in which there was no damage. Results Allowing for the direct effect of holes, the effect on use, and the effect on attrition, 18% of the impact on vectorial capacity was estimated to be lost because of damage. The estimated median lifetime of the LLINs was 2.9 years, but this was extended to 5.7 years in the counterfactual without physical damage. Nets that were in use were more likely to be in a damaged state than unused nets but use made little direct difference to LLIN lifetimes. Damage was reported as the reason for attrition for almost half of attrited nets, but the model estimated that almost all attrited nets had suffered some damage before attrition. Conclusions Full quantification of the effects of damage will require measurement of the supply of new nets and of household stocks of unused nets, and also of their impacts on both net use and retention. The timing of mass distribution campaigns is less important than ensuring sufficient supply. In the Kenyan setting, nets acquired damage rapidly once use began and the damage led to rapid attrition. Increasing the robustness of nets could substantially increase their lifetime and impact but the impact of LLIN programmes on malaria transmission is ultimately limited by levels of use. Longitudinal analyses of net integrity data from different settings are needed to determine the importance of physical damage to nets as a driver of attrition and non-use, and the importance of frequent use as a cause of physical damage in different contexts.

Predisposing Factors to Lymphatic Filariasis among Residents in Igbo-Eze North; an Endemic Area in Nigeria

Background: The study on lymphatic filariasis (LF) in Igbo-Eze North was conducted to determine the prevalence and predisposing factors to LF among its residents between May and October 2018. Methods: A total of 201 residents who have lived in the area for at least one year were recruited. They were stratified according to age, gender and occupation, and were clinically examined firstly by rapid assessment method for any lymphoedema and hydrocele. At recruitment, blood samples were collected from all volunteered participants for LF test. In addition, demographic information and risk factors of the respondents were captured using a structured questionnaire by oral interview. Results: The overall prevalence for LF was 84 (41.8%). Furthermore, LF prevalence was significant (P < 0.05) in all the studied communities: 61.5% in Umuogbuagu, 48.1% in Aguibege, 32.7% in Umuagama and 21.7% in Umuopu. The sex-related prevalence of LF was higher among females than males, with slight significant difference (P = 0.046). In relation to age and occupation, higher prevalences (P < 0.0001) were recorded among older (≥ 50 years) subjects (49, 61.2%) and traders (55, 57.9%) respectively. The risk associated with LF implicated lack of knowledge, non-use of mosquito nets, as well as visit and proximity to water bodies as major predispositions (P < 0.05). Conclusion: The prevalence of LF in this study was high. Higher prevalence was among females, older people and traders. Notable risks to the disease outcome are environmental, attitudinal and occupational with chances of scaled up prevalence and burden overtime.

The moderated mediation role of malaria knowledge and household income in the relationship between insecticidetreated mosquito nets and malaria prevalence

Objectives: The study was premised on the notion that insecticidetreated mosquito nets are positively related to malaria prevalence, and that knowledge mediates the relationship between insecticide-treated mosquito nets and malaria. Furthermore, household income was hypothesized to have a moderating effect on the direct and indirect relationships (through malaria knowledge) between insecticide-treated mosquito nets and the prevalence of malaria. Methods: The hypothesized relationships were examined using panel data collected from ten regions of Ethiopia during 2011 – 2015. Structural equation modeling and the random effect model were used to test the hypotheses. Statistical analyses were performed using Stata version 13.0. Results: The results were consistent with our proposed hypotheses, showing a significant relationship between the research variables. The findings suggest that malaria knowledge contributes to improving the relationship between insecticide-treated mosquito nets and malaria prevalence. A positively significant indirect effect (β = 0.47, p = 0.003) as well as direct effect (β = 0.28, p = 0.001) was revealed in the study. Furthermore, a positive impact of household income in strengthening the relationship between insecticide-treated mosquito nets and malaria through knowledge reported, with a considerable value (β = 0.13, p = 0.000). The result also reveals differences in the outcome of malaria prevalence at different levels of household income category, where the indirect effect of insecticide-treated mosquito nets on malaria prevalence via malaria knowledge was positive and significant for households under the second-level income category (β = 0.15, p = 0.000). Conversely, the indirect effect of insecticide-treated mosquito nets on malaria prevalence via malaria knowledge was negative for the high level-income category (β = -0.14, p = 0.022). Besides, insignificant and negative relationships were reported for households under low-level income categories (β = 0.024, p = 0.539). Conclusion: The findings are potentially useful for the health sector to ensure success in infectious disease prevention and control, particularly malaria, and to explain how various factors contribute to the relationship. Keywords: malaria; insecticide-treated mosquito nets; malaria knowledge; household-income; structural equation modelling.

The Moderated Mediation Role of Malaria Knowledge and Household Income in the Relationship between Insecticide-Treated Mosquito Nets and Malaria

Objectives: The study was premised on the notion that insecticide-treated mosquito nets are positively related to malaria prevalence, and that knowledge mediates the relationship between insecticide-treated mosquito nets and malaria. Furthermore, household income was hypothesized to have a moderating effect on the direct and indirect relationships (through malaria knowledge) between insecticide-treated mosquito nets and the prevalence of malaria. Methods: The hypothesized relationships were examined using panel data collected from ten regions of Ethiopia during 2011-2015. Structural equation modeling and the random effect model were used to test the hypotheses. Statistical analyses were performed using Stata version 13.0. Results: The results were consistent with our proposed hypotheses, showing a significant relationship between the research variables. The findings suggest that malaria knowledge contributes to improving the relationship between insecticide-treated mosquito nets and malaria prevalence. A positively significant indirect effect (β = 0.47, p = 0.003) as well as direct effect (β = 0.28, p = 0.001) was revealed in the study. Furthermore, a positive impact of household income in strengthening the relationship between insecticide-treated mosquito nets and malaria through knowledge was reported, with a considerable value (β = 0.13, p = 0.000). The result also reveals differences in the outcome of malaria prevalence at different levels of household income category, where the indirect effect of insecticide-treated mosquito nets on malaria prevalence via malaria knowledge was positive and significant for households under the second-level income category (β = 0.15, p = 0.000). Conversely, the indirect effect of insecticide-treated mosquito nets on malaria prevalence via malaria knowledge was negative for the high level-income category (β = -0.14, p = 0.022). Besides, insignificant and negative relationships were reported for households under low-level income categories (β = 0.024, p = 0.539). Conclusion: The findings are potentially useful for the health sector to ensure success in infectious disease prevention and control, particularly malaria, and to explain how various factors contribute to the relationship.

Exploring Socio-Economic Impact of Chikungunya Fever in Dhaka: A Statistical Modeling Approach

The study mainly focused on the socio-economic impact rather than the biological significance of Chikungunya fever in Dhaka city. The socio-economic impact had been measured mainly upon family cost and absence in the working place. It had been investigated whether a percentage of monthly family income spend on monthly expenditure changes due to Chikungunya incidence. Also, another inquiry was made about the productivity of the respondents, which had been measured by the number of absences in working place. The study considered primary data of 272 affected and 272 unaffected respondents from Dhaka city and found no significant association of family cost with Chikungunya incidence but the loss of productivity turned out as statistically significant. Another important objective was to explore potential determinants of Chikungunya. It is found that some patients use no preventive approach, while the majority use mosquito nets as a preventive approach. The respondents suggest themselves mostly to keep the drain free from blockage, regular changing water from plant containers, and use mosquito repellent and net. They seek from the government mostly to provide regular mosquito spray, develop drainage systems, and remove blocked water sources. Chikungunya has no direct economic impact on a family. But, as it affects productivity, it affects the national economy to a great extent.

Phytochemistry and GC-MS screening and biocidal potentiality of clove (Syzygium aromaticum L.) pods against mosquitos’ larvae

Many measures have been used for mosquito control, including the elimination of breeding places, exclusion via window screens and mosquito nets in addition to natural products including clove (Syzygium aromaticum). This study was run at University of Gezira, Sudan, to run phytochemical and GC-MS screening for clove pods before used it as mosquito control agent. The standard methods, materials and devices were used to screen the phytochemical components and the chemical constituents (GC-MS). The WHO protocol for testing the susceptibility of mosquito’s larvae to insecticides was followed in bioassay. The aqueous and the ethanol extracts from clove pods were prepared and used against Anopheles, Culex and Aedes larvae. The results showed that, Aedes mosquito was relatively more susceptible (LC50= 498 mg/L) to clove aqueous extract than Anopheles (LC50= 561 mg/L) and Culex (LC50= 615 mg/L), and similar findings were observed for clove pods ethanol extract, which is relatively more potent than the aqueous extract. The biocidal activity can be attributed to the presence of the detected saponins, flavonoids, tannins and alkaloids. The GC-MS for the ethanol extract showed that, the principal compounds were Eugenol (81%) and caryophyllene (4.65%). Further studies should be run to improve knowledge about how to use this natural product in more economic trends.

Anemia during pregnancy: Epidemiological, clinical and therapeutic aspects at the obstetric gynecology department of Labe Regional Hospital, Guinee

Objectives: Calculate the frequency of anemia during pregnancy, describe the epidemiological profile and describe the management of anemia during pregnancy in the gynecology-obstetrics Department of the Labé regional hospital. Methodology: This was a prospective, descriptive study lasting six (6) months from September 1, 2020 to February 28, 2021 All pregnant women who developed anemia and treated were considered for the study in the service. All pregnant women without anemia were included. Our data were analyzed by Epi-Info version 7.2.2.6 software, entered and presented by Pack office 2016 software. Results: The frequency of anemia in pregnant women was 27.61% in the department. The epidemiological profile of anemia in pregnant women at the Labé regional hospital was that of: a woman aged 25-29 (30.63%), housewives (72.97%), women not attending school (66, 67 %.), those Coming from the rural areas (76.58%), married women (100%), pauciparous (29.73%) and only 14.42% did not carry out an antenatal consultation. The anemia occurred in the 3rd trimester (76.58 %,). The main reason for consultation was paleness of the conjunctivae and integuments (92.79%) and malaria the dominant antecedent (60.36%). Severe anemia was the most dominant clinical form (54.96%). Transfusion was performed in 54.95% of women with anemia. The average length of stay was 4.9 days with extremes of 1 and 10 days. The maternal prognosis was favorable in 96.40% with a maternal death rate of 3.60%. Conclusion: Anemia is a major problem for pregnant women in the Gynecology-Obstetrics department at the Labé regional hospital. The improvement of the maternal prognosis would pass by chemoprophylaxis of malaria, the use of impregnated mosquito nets with long-lasting insecticide (LLINs), the prevention of intestinal parasitoses, the regular administration of iron, folic acid and the provision of quality prenatal consultation.