Sirolimus-eluting stents: opposite in vitro effects on the clonogenic cell potential on a long-term exposure

Hydatidosis is characterized by the long-term coexistence of larvaEchinococcus granulosusand its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO2−), nitrate (NO3−) and peroxynitrite (ONOO−) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity ofNO2−andONOO−24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated thatONOO−is more cytotoxic thanNO2−. In contrast,NO3−has no effect. These results suggest possible involvement ofNO2−andONOO−in antihydatic action and point the efficacy of these metabolites as scolicidal agents.

Download Full-text

In vitro effects of CaO nanoparticles on Triticale callus exposed to short and long-term salt stress

Plant Cell Reports ◽

10.1007/s00299-020-02613-0 ◽

2020 ◽

Author(s):

Büşra Yazıcılar ◽

Fatma Böke ◽

Azize Alaylı ◽

Hayrunisa Nadaroglu ◽

Semin Gedikli ◽

...

Keyword(s):

Salt Stress ◽

Short And Long Term ◽

In Vitro Effects

Download Full-text

Short-term effect of aldosterone on NEM-sensitive ATPase in rat collecting tubule

AJP Renal Physiology ◽

10.1152/ajprenal.1989.257.2.f177 ◽

1989 ◽

Vol 257 (2) ◽

pp. F177-F181 ◽

Cited By ~ 4

Author(s):

C. Khadouri ◽

S. Marsy ◽

C. Barlet-Bas ◽

A. Doucet

Keyword(s):

Atpase Activity ◽

Affinity Constant ◽

Short Term ◽

Collecting Tubule ◽

Lag Period ◽

In Vitro Effects ◽

Collecting Tubules

Because previous studies indicated that in the collecting tubule, N-ethylmaleimide (NEM)-sensitive ATPase, the biochemical equivalent of the proton pump, is controlled by mineralocorticoids in the long term, the present study was designed to investigate whether such control also exists in the short term. Therefore we investigated the in vivo and in vitro effects of aldosterone on the enzyme activity in cortical and outer medullary collecting tubules (CCT and MCT, respectively) from adrenalectomized rats. Administration of aldosterone (10 micrograms/kg body wt) markedly stimulated NEM-sensitive ATPase activity in the CCT and MCT within 3 h. Similarly, incubating CCT or MCT for 3 h in the presence of 10(-8) M aldosterone enhanced NEM-sensitive ATPase activity up to values similar to those previously measured in the corresponding nephron segments of normal rats. In vitro stimulation of NEM-sensitive ATPase was dose dependent in regard to aldosterone (apparent affinity constant approximately 10(-9) M), appeared after a 30-min lag period, and reached its maximum after 2-2.5 h. Finally, actinomycin D and cycloheximide totally abolished the in vitro action of aldosterone, demonstrating the involvement of protein synthesis in this process.

Download Full-text

Long-term delivery of superoxide dismutase and catalase entrapped in poly(lactide-co-glycolide) microspheres: In vitro effects on isolated neonatal porcine pancreatic cell clusters

Journal of Controlled Release ◽

10.1016/j.jconrel.2005.05.021 ◽

2005 ◽

Vol 107 (1) ◽

pp. 65-77 ◽

Cited By ~ 39

Author(s):

S GIOVAGNOLI ◽

G LUCA ◽

I CASABURI ◽

P BLASI ◽

G MACCHIARULO ◽

...

Keyword(s):

Superoxide Dismutase ◽

Pancreatic Cell ◽

Cell Clusters ◽

Term Delivery ◽

In Vitro Effects

Download Full-text

Alternative mechanisms with and without steel factor support primitive human hematopoiesis

Blood ◽

10.1182/blood.v81.6.1465.1465 ◽

1993 ◽

Vol 81 (6) ◽

pp. 1465-1470 ◽

Cited By ~ 56

Author(s):

HJ Sutherland ◽

DE Hogge ◽

D Cook ◽

CJ Eaves

Keyword(s):

Hematopoietic Stem ◽

Steel Factor ◽

Stem Cell Maintenance ◽

Murine Fibroblasts ◽

Normal Human ◽

Clonogenic Cell ◽

First Time ◽

Stimulating Factor

Abstract As a first approach to defining the molecular requirements for supporting human hematopoietic stem cell maintenance and differentiation in vitro, we have analyzed and compared the ability of various factors to support the maintenance and initial differentiation of human long-term culture-initiating cells (LTC-ICs), a distinct, rare primitive hematopoietic cell type whose progeny after 5 weeks include cells detectable as colony-forming cells. Normal human marrow cells highly enriched in LTC-ICs (approximately 1% pure) were placed in cultures containing either preestablished, irradiated human marrow adherent feeder layers, or feeders consisting of Steel factor-deficient SI/SI, or normal +/+ murine fibroblasts, or no feeders. In some groups, either Steel factor alone, granulocyte colony-stimulating factor (G- CSF) and interleukin-3 (IL-3), or all three factors combined were also added repeatedly. SI/SI murine fibroblasts were equivalent to +/+ controls and to normal human marrow feeders in supporting both LTC-IC maintenance and clonogenic cell output over a 5-week period. Soluble Steel factor alone could, however, effectively substitute for human marrow feeders to support LTC-IC maintenance, although clonogenic cell output was markedly reduced under these conditions. Conversely, soluble Steel factor with G-CSF and IL-3 or with feeders (or all together) did not further enhance (or depress) LTC-IC maintenance, although under these conditions clonogenic cell output was markedly increased. These findings confirm previous evidence that LTC-IC maintenance and clonogenic cell production are differentially regulated and show for the first time that LTC-IC maintenance can be supported by different nonsynergizing factors that may, but need not, include Steel factor.

Download Full-text

In vivo blockade of TNF-α in patients with rheumatoid arthritis: Indication for tachyphylaxis in long term ex vivo and in vitro effects after repeated infusion of chimeric monoclonal antibody cA2

Immunology Letters ◽

10.1016/s0165-2478(97)85159-0 ◽

1997 ◽

Vol 56 ◽

pp. 42

Author(s):

M. Grünke ◽

H.-M. Lorenz ◽

T. Hieronymus ◽

T. Geiler ◽

C. Antoni ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Monoclonal Antibody ◽

Ex Vivo ◽

Chimeric Monoclonal Antibody ◽

Tnf Α ◽

In Vitro Effects

Download Full-text

IN VITRO EFFECTS OF ISOQUERCITRIN ON SELECTED VITALITY MARKERS OF BOVINE SPERMATOZOA

Archives of Ecotoxicology ◽

10.36547/ae.2019.1.2.18-23 ◽

2019 ◽

Vol 1 (2) ◽

pp. 18-23

Author(s):

Filip Benko ◽

Hana Greifová ◽

Eva Tvrdá

Keyword(s):

Physiological Saline ◽

Biological Properties ◽

Computer Assisted ◽

Movement Activity ◽

Spermatozoa Motility ◽

Physiological Saline Solution ◽

Bovine Spermatozoa ◽

In Vitro Effects

The aim of this study was to evaluate the dose- and time-dependent in vitroeffects of isoquercitrin (ISO), a natural flavonoid with numerous biological properties on bovine spermatozoa during three different time periods (0 h, 2 h, 24 h). Bovine semen samples were diluted and cultivated in physiological saline solution containing 0.5% DMSO together with 200, 100, 50, 10, 5 and 1 μmol/L ISO. Spermatozoa motility was measured using the HTM IVOS CASA (Computer Assisted Semen Analyzer) system. The viability of spermatozoa was assessed by the metabolic (MTT) assay, production of superoxide radicals was quantified using the nitroblue tetrazolium (NBT) test, and chemiluminescence was used to evaluate the generation of reactive oxygen species (ROS). The results of the movement activity showed a significant increase in the motility during long term cultivation in case of concentrationsranging between 5 and 50 μmol/L ISO (P<0.05; 24 h). At the same time, supplementation of several concentrations of ISO led to a significant preservation of the cell viability (P<0.05 in the case of 50 μmol/L, P<0.01 with respect to 1 and 5 μmol/L, and P<0.001 in relation to 10 μmol/L; 24 h). ISO addition at 10 and 50 μmol/L also provided a significantly higher protection against superoxide (P<0.05) and ROS (P<0.001) overgeneration after a 24 h cultivation. We may suggest that supplementation of ISO to bovine spermatozoa, particularly at concentrations ranging between 10 and 50 μmol/L, may offer protection to the motility, viability and oxidative status of the sperm cells, particularly notable at 24 h.

Download Full-text

Phenotypic and functional characterization of competitive long-term repopulating hematopoietic stem cells enriched from 5-fluorouracil- treated murine marrow

Blood ◽

10.1182/blood.v81.9.2310.bloodjournal8192310 ◽

1993 ◽

Vol 81 (9) ◽

pp. 2310-2320 ◽

Cited By ~ 3

Author(s):

SJ Szilvassy ◽

S Cory

Keyword(s):

Stem Cells ◽

Hematopoietic Stem Cells ◽

Functional Characterization ◽

Significant Proportion ◽

Cell Types ◽

Hematopoietic Stem ◽

Clonogenic Cell

Lymphomyeloid stem cells from the bone marrow of C57BL/6 mice treated with 5-fluorouracil (5-FU) were characterized with respect to 12 parameters using fluorescence-activated cell sorting and a competitive long-term repopulation assay. Stem cells were larger than lymphocytes and exhibited side light-scatter characteristic of blast cells. Most expressed low levels of Thy-1.2, high levels of Sca-1 (Ly6-A/E), H-2Kb, and AA4.1 antigens and stained brightly with rhodamine-123. Significantly, most long-term repopulating cells also expressed CD4, some at high density. In addition, a significant proportion displayed low to medium levels of the “lineage-specific” markers CD45R (B220), Gr- 1, and TER-119. A simple and rapid multiparameter sorting procedure enriched the stem cells 100-fold and substantially removed most other clonogenic cell types, including day 12 spleen colony-forming cells. Cells able to generate cobblestone colonies on stromal cells in vitro were co-enriched. Lethally irradiated mice transplanted with limiting numbers of the sorted stem cells did not survive unless cotransplanted with “compromised” marrow cells prepared by prior serial transplantation and shown to be depleted of long-term repopulating activity. A significant number of recipients transplanted with 25 to 100 sorted cells contained donor-derived B and T lymphocytes and granulocytes in their peripheral blood for at least 6 months. Limiting dilution analysis in vivo indicated that the frequency of competitive long-term repopulating units (CRU) in the sorted population was at least 1 in 60 cells. The calculated frequency of CRU was largely independent of the time of recipient analysis between 10 and 52 weeks, indicating that highly enriched stem cells can be recruited relatively early in certain transplant settings. This simple enrichment and assay strategy for repopulating hematopoietic stem cells should facilitate further analysis of their regulation in vivo.

Download Full-text