Genetic Relationship of Transcriptional Factor Genes in the Regulation of Cells Death in Arabidopsis: A Review

The leaf yellowing is the first visible sign of senescence, which starts at the margins of the leaf and progresses to the blade. Although, transcriptional factor family genes generally encode meticulous regulators which perform a range of functions in turns regulating the physiological and developmental mechanism of plant stature. However, the genetic relationship of TFs genes in regulating the cell death of Arabidopsis is well not understood to date. TFs family in a plant regulates various developmental and stress responses in underline pathways. In our review we observed the genetic relationship of TFs genes in regulations of cell death in Arabidopsis. Given that, programmed cell death (PCD) being an active process that includes the expression of hundreds of genes. It is speculated that many TFs are involved in the core elements of the regulatory network. There are only a few factors that are being demonstrated in involving the regulation of cell death, by evaluating the leaf senescence appearances of knocking of mutants and by identifying downstream target genes. In this review, we have focused on the manifold roles of TFs during genetic relationships and the regulation of cell death in Arabidopsis. We also deliberated how the transcription factors family gene regulates the cells’ death by different hormonal stress, environmental strain and their role in retrograde signaling. For deep understanding of regulatory molecular mechanisms of cell death in the plant, future research may be hypothesized to collect appropriate evidence and a detailed study may be implemented on the upstream pathway with a specific targeted gene that recognizes the stress signals involved in cell death in plants. Also, crosstalk between mitochondria and chloroplast is mainly being focused to better understand the regulations of cell death in plants. Present review concludes that regulating the cell death of Arabidopsis is very important for meeting future global food needs, crop yields. Overexpression of ERF transcription factors genes relating cell death of Arabidopsis confers broad-spectrum resistance to pathogens and other abiotic stresses and can also make transgenic plants resistant to drought, salinity and freezing.

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LONP1 and ClpP cooperatively regulate mitochondrial proteostasis for cancer cell survival

Oncogenesis ◽

10.1038/s41389-021-00306-1 ◽

2021 ◽

Vol 10 (2) ◽

Author(s):

Yu Geon Lee ◽

Hui Won Kim ◽

Yeji Nam ◽

Kyeong Jin Shin ◽

Yu Jin Lee ◽

...

Keyword(s):

Cell Death ◽

Cell Growth ◽

Cell Survival ◽

Cancer Cell ◽

Stress Responses ◽

Molecular Mechanisms ◽

Tca Cycle ◽

Mitochondrial Matrix ◽

Mitochondrial Functions ◽

Cancer Cell Survival

AbstractMitochondrial proteases are key components in mitochondrial stress responses that maintain proteostasis and mitochondrial integrity in harsh environmental conditions, which leads to the acquisition of aggressive phenotypes, including chemoresistance and metastasis. However, the molecular mechanisms and exact role of mitochondrial proteases in cancer remain largely unexplored. Here, we identified functional crosstalk between LONP1 and ClpP, which are two mitochondrial matrix proteases that cooperate to attenuate proteotoxic stress and protect mitochondrial functions for cancer cell survival. LONP1 and ClpP genes closely localized on chromosome 19 and were co-expressed at high levels in most human cancers. Depletion of both genes synergistically attenuated cancer cell growth and induced cell death due to impaired mitochondrial functions and increased oxidative stress. Using mitochondrial matrix proteomic analysis with an engineered peroxidase (APEX)-mediated proximity biotinylation method, we identified the specific target substrates of these proteases, which were crucial components of mitochondrial functions, including oxidative phosphorylation, the TCA cycle, and amino acid and lipid metabolism. Furthermore, we found that LONP1 and ClpP shared many substrates, including serine hydroxymethyltransferase 2 (SHMT2). Inhibition of both LONP1 and ClpP additively increased the amount of unfolded SHMT2 protein and enhanced sensitivity to SHMT2 inhibitor, resulting in significantly reduced cell growth and increased cell death under metabolic stress. Additionally, prostate cancer patients with higher LONP1 and ClpP expression exhibited poorer survival. These results suggest that interventions targeting the mitochondrial proteostasis network via LONP1 and ClpP could be potential therapeutic strategies for cancer.

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Genetic Analysis of Tropical Orchid Hybrids (Dendrobium) with Fluorescence Amplified Fragment-length Polymorphism (AFLP)

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.128.5.0731 ◽

2003 ◽

Vol 128 (5) ◽

pp. 731-735 ◽

Cited By ~ 6

Author(s):

N. Xiang ◽

Y. Hong ◽

L.T. Lam-Chan

Keyword(s):

Length Polymorphism ◽

Genetic Relationship ◽

Fragment Length Polymorphism ◽

Genetic Relationships ◽

Aflp Analysis ◽

Tropical Orchid ◽

Trade Names ◽

Relationship Of ◽

Intensive Breeding ◽

Different Parts

Intensive breeding activities of tropical orchids have given rise to many hybrids, among which genetic relationships are difficult to evaluate due to free interbreeding of different species in the same genus or even from different genera, the use of hybrids for further breeding, use of abbreviated or trade names and sometimes intentional non-disclosure of parentage for commercial considerations. We have subjected 43 popular commercial Dendrobium hybrids to fluorescence amplified length polymorphism (AFLP) analysis and their genetic relationship was estimated. The hybrids bearing flowers of similar shapes and colors were clustered into five groups. Each hybrid tested had a distinct AFLP fingerprint profile except the tissue culture mutants. Sibling hybrids were closely clustered (with genetic distance <0.09) followed by those sharing one parent. These results suggest that AFLP fingerprint profiling gives accurate and objective estimation of genetic relationship of the Dendrobium hybrids tested. Our study also found that the AFLP fingerprint profiles were uniform in different parts of tested plants, stable among individuals in vegetatively propagated populations throughout different growth periods. We conclude that AFLP fingerprint profiling has the potential to be an integral part of current new plant varieties protection sytems.

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Genetic Relationship of Ornamental Peach Using Amplified Fragments Length Polymorphism (AFLPs) Fingerprints

HortScience ◽

10.21273/hortsci.39.4.888b ◽

2004 ◽

Vol 39 (4) ◽

pp. 888B-888

Author(s):

Dongyan Hu* ◽

Zuoshuang Zhang ◽

Donglin Zhang ◽

Qixiang Zhang

Keyword(s):

Genetic Relationship ◽

Prunus Persica ◽

Urban Landscape ◽

Genetic Relationships ◽

Base Pairs ◽

Molecular Systematic ◽

Germplasm Identification ◽

The Us ◽

Relationship Of ◽

Close Genetic Relationship

Ornamental peach (Prunus persica (L.) Batsch) is native to China. The ornamental value of peach is gaining popularity for its use in urban landscape and everyday gardens. However, the genetic relationship among ornamental peach cultivars is not clear, which limits the further studies of its molecular systematic. A sample of 51 cultivars of ornamental peach, originated from P. persica and Prunus davidiana, had been studied by using AFLPs. All samples were collected from China, Japan, and the US. A total of 275 useful markers between 75 to 500 base pairs were generated from 6 EcoRI/MseI AFLP primer combinations. Among them, 93% of bands were polymorphic markers. Total markers for each cultivar ranged form 90 to 140, and the average number of markers for each cultivar was 120. Two distinguished clad generated from PAUP-UPGMA tree. P. davidiana, as a species, was apparently an out-group to P. persica, which implied that P. davidiana was far away genetically from ornamental peach (P. persica). Within P. persica clad, 15 out of 17 upright ornamental peach cultivars in this study were grouped to one clad, which indicated cultivars that with upright growth habit had close genetic relationship. Five dwarf cultivars were grouped to one clad, with 81% bootstrap supported. The genetic relationships between these five dwarfs were much closer than any other cultivars, and showed that they probably derived from the similar gene pool. The results demonstrated that AFLP are powerful markers for revealing genetic relationships in ornamental peach. The genetic relationships among ornamental cultivars established in this study could help future ornamental peach germplasm identification, conservation, and new cultivars development.

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Enhancement of the Initial Rate of Ethanol Fermentation Due to Dysfunction of Yeast Stress Response Components Msn2p and/or Msn4p

Applied and Environmental Microbiology ◽

10.1128/aem.01869-10 ◽

2010 ◽

Vol 77 (3) ◽

pp. 934-941 ◽

Cited By ~ 48

Author(s):

Daisuke Watanabe ◽

Hong Wu ◽

Chiemi Noguchi ◽

Yan Zhou ◽

Takeshi Akao ◽

...

Keyword(s):

Gene Expression ◽

Saccharomyces Cerevisiae ◽

Transcription Factors ◽

Stress Response ◽

Stress Responses ◽

Ethanol Fermentation ◽

Molecular Mechanisms ◽

Initial Rate ◽

Response Elements ◽

High Concentrations

ABSTRACTSake yeasts (strains ofSaccharomyces cerevisiae) produce high concentrations of ethanol in sake fermentation. To investigate the molecular mechanisms underlying this brewing property, we compared gene expression of sake and laboratory yeasts in sake mash. DNA microarray and reporter gene analyses revealed defects of sake yeasts in environmental stress responses mediated by transcription factors Msn2p and/or Msn4p (Msn2/4p) and stress response elements (STRE). Furthermore, we found that dysfunction ofMSN2and/orMSN4contributes to the higher initial rate of ethanol fermentation in both sake and laboratory yeasts. These results provide novel insights into yeast stress responses as major impediments of effective ethanol fermentation.

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Protective Effect of Fucoidan against MPP+-Induced SH-SY5Y Cells Apoptosis by Affecting the PI3K/Akt Pathway

Marine Drugs ◽

10.3390/md18060333 ◽

2020 ◽

Vol 18 (6) ◽

pp. 333

Author(s):

Huaide Liu ◽

Jing Wang ◽

Quanbin Zhang ◽

Lihua Geng ◽

Yue Yang ◽

...

Keyword(s):

Cell Death ◽

Protective Effect ◽

Molecular Mechanisms ◽

Caspase 3 ◽

Pi3k Inhibitor ◽

Neuroprotective Activity ◽

Akt Pathway ◽

Cellular Model ◽

Pi3k Inhibitor Ly294002 ◽

Cells Death

The main pathologic changes of the Parkinson’s disease (PD) is dopaminergic (DA) neurons lost. Apoptosis was one of the important reasons involved in the DA lost. Our previous study found a fucoidan fraction sulfated heterosaccharide (UF) had neuroprotective activity. The aim of this study was to clarify the mechanism of UF on DA neurons using human dopaminergic neuroblastoma (SH-SY5Y) cells a typical as a PD cellular model. Results showed that UF prevented MPP+-induced SH-SY5Y cells apoptosis and cell death. Additionally, UF pretreated cells increased phosphorylation of Akt, PI3K and NGF, which means UF-treated active PI3K–Akt pathway. Moreover, UF treated cells decreased the expression of apoptosis-associated protein, such as the ratio of Bax/Bcl-2, GSK3β, caspase-3 and p53 nuclear induced by MPP+. This effect was partially blocked by PI3K inhibitor LY294002. Our data suggested that protective effect of UF against MPP+-induced SH-SY5Y cells death by affecting the PI3K–Akt pathway. These findings contribute to a better understanding of the critical roles of UF in treating PD and may elucidate the molecular mechanisms of UF effects in PD.

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Identification of ferroptosis-associated genes exhibiting altered expression in pulmonary arterial hypertension

Mathematical Biosciences and Engineering ◽

10.3934/mbe.2021377 ◽

2021 ◽

Vol 18 (6) ◽

pp. 7619-7630

Author(s):

Fan Zhang ◽

◽

Hongtao Liu ◽

Keyword(s):

Cell Death ◽

Pulmonary Arterial Hypertension ◽

Arterial Hypertension ◽

Stress Responses ◽

Molecular Mechanisms ◽

Expression Profiles ◽

Pathway Enrichment Analysis ◽

Altered Expression ◽

Network Analyses ◽

Pulmonary Arterial

<abstract> <p>Pulmonary arterial hypertension (PAH) is a life-threatening illness and ferroptosis is an iron-dependent form of regulated cell death, driven by the accumulation of lipid peroxides to levels that are sufficient to trigger cell death. However, only few studies have examined PAH-associated ferroptosis. In the present study, lung samples mRNA expression profiles (derived from 15 patients with PAH and 11 normal controls) were downloaded from a public database, and 514 differentially expressed genes (DEGs) were identified using the Wilcoxon rank-sum test and weighted gene correlation network analyses. These DEGs were screened for ferroptosis-associated genes using the FerrDb database: eight ferroptosis-associated genes were identified. Finally, the construction of gene-microRNA (miRNA) and gene-transcription factor (TF) networks, in conjunction with gene ontology and biological pathway enrichment analysis, were used to inform hypotheses regarding the molecular mechanisms underlying PAH-associated ferroptosis. Ferroptosis-associated genes were largely involved in oxidative stress responses and could be regulated by several identified miRNAs and TFs. This suggests the existence of modulatable pathways that are potentially involved in PAH-associated ferroptosis. Our findings provide novel directions for targeted therapy of PAH in regard to ferroptosis. These findings may ultimately help improve the therapeutic outcomes of PAH.</p> </abstract>

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Genetic Relationship of Ornamental Peach Determined Using AFLP Markers

HortScience ◽

10.21273/hortsci.40.6.1782 ◽

2005 ◽

Vol 40 (6) ◽

pp. 1782-1786 ◽

Cited By ~ 17

Author(s):

Dongyan Hu ◽

Zuoshuang Zhang ◽

Donglin Zhang ◽

Qixiang Zhang ◽

Jianhua Li

Keyword(s):

Genetic Relationship ◽

Prunus Persica ◽

Growth Habit ◽

Genetic Relationships ◽

Aflp Markers ◽

Urban Landscapes ◽

Gene Pools ◽

Base Pairs ◽

Relationship Of ◽

Close Genetic Relationship

Ornamental peach (Prunus persica (L.) Batsch) is a popular plant for urban landscapes and gardens. However, the genetic relationship among ornamental peach cultivars is unclear. In this report, a group of 51 ornamental peach taxa, originated from P. persica and P. davidiana (Carr.) Franch., has been studied using AFLPs. The samples were collected from China, Japan, and US. A total of 275 useful markers ranging in size from 75 to 500 base pairs were generated using six EcoRI/MseI AFLP primer pairs. Among them, 265 bands were polymorphic. Total markers for each taxon ranged from 90 to 140 with an average of 120. Two clades were apparent on the PAUP–UPGMA tree with P. davidiana forming an outgroup to P. persica, indicates that P. davidiana contributed less to the ornamental peach gene pools. Within P. persica clade, 18 out of 20 upright ornamental peach cultivars formed a clade, which indicated that cultivars with upright growth habit had close genetic relationship. Five dwarf cultivars were grouped to one clade, supported by 81% bootstrap value, indicating that they probably derived from a common gene pool. These results demonstrated that AFLP markers are powerful for determining genetic relationships in ornamental peach. The genetic relationships among ornamental cultivars established in this study could be useful in ornamental peach identification, conservation, and breeding.

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RNA Biological Characteristics at the Peak of Cell Death in Different Hereditary Retinal Degeneration Mutants

Frontiers in Genetics ◽

10.3389/fgene.2021.728791 ◽

2021 ◽

Vol 12 ◽

Author(s):

Chunling Wei ◽

Yan Li ◽

Xiaoxiao Feng ◽

Zhulin Hu ◽

François Paquet-Durand ◽

...

Keyword(s):

Cell Death ◽

Transcription Factors ◽

Network Analysis ◽

Retinal Degeneration ◽

Cell Activation ◽

Molecular Mechanisms ◽

Photoreceptor Cell ◽

Predictive Biomarkers ◽

Retinal Tissue ◽

Inherited Retinal Degeneration

Purpose: The present work investigated changes in the gene expression, molecular mechanisms, and pathogenesis of inherited retinal degeneration (RD) in three different disease models, to identify predictive biomarkers for their varied phenotypes and to provide a better scientific basis for their diagnosis, treatment, and prevention.Methods: Differentially expressed genes (DEGs) between retinal tissue from RD mouse models obtained during the photoreceptor cell death peak period (Pde6brd1 at post-natal (PN) day 13, Pde6brd10 at PN23, Prphrd2 at PN29) and retinal tissue from C3H wild-type mice were identified using Illumina high-throughput RNA-sequencing. Co-expression gene modules were identified using a combination of GO and KEGG enrichment analyses and gene co-expression network analysis. CircRNA-miRNA-mRNA network interactions were studied by genome-wide circRNA screening.Results:Pde6brd1, Pde6brd10, and Prphrd2 mice had 1,926, 3,096, and 375 DEGs, respectively. Genes related to ion channels, stress, inflammatory processes, tumor necrosis factor (TNF) production, and microglial cell activation were up-regulated, while genes related to endoplasmic reticulum regulation, metabolism, and homeostasis were down-regulated. Differential expression of transcription factors and non-coding RNAs generally implicated in other human diseases was detected (e.g., glaucoma, diabetic retinopathy, and inherited retinal degeneration). CircRNA-miRNA-mRNA network analysis indicated that these factors may be involved in photoreceptor cell death. Moreover, excessive cGMP accumulation causes photoreceptor cell death, and cGMP-related genes were generally affected by different pathogenic gene mutations.Conclusion: We screened genes and pathways related to photoreceptor cell death. Additionally, up-stream regulatory factors, such as transcription factors and non-coding RNA and their interaction networks were analyzed. Furthermore, RNAs involved in RD were functionally annotated. Overall, this study lays a foundation for future studies on photoreceptor cell death mechanisms.

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Emotions and health: The impact of emotions, emotions regulation, music, and acculturation on health

Zeitschrift für Gesundheitspsychologie ◽

10.1026/0943-8149.16.3.112 ◽

2008 ◽

Vol 16 (3) ◽

pp. 112-115 ◽

Cited By ~ 1

Author(s):

Stephan Bongard ◽

Volker Hodapp ◽

Sonja Rohrmann

Keyword(s):

Stress Responses ◽

Physiological Stress ◽

Anger Expression ◽

Domain Specific ◽

Cardiovascular Stress ◽

Expression Behavior ◽

Cardio Vascular ◽

And Coping ◽

Relationship Of ◽

The Impact

Abstract. Our unit investigates the relationship of emotional processes (experience, expression, and coping), their physiological correlates and possible health outcomes. We study domain specific anger expression behavior and associated cardio-vascular loads and found e.g. that particularly an open anger expression at work is associated with greater blood pressure. Furthermore, we demonstrated that women may be predisposed for the development of certain mental disorders because of their higher disgust sensitivity. We also pointed out that the suppression of negative emotions leads to increased physiological stress responses which results in a higher risk for cardiovascular diseases. We could show that relaxation as well as music activity like singing in a choir causes increases in the local immune parameter immunoglobuline A. Finally, we are investigating connections between migrants’ strategy of acculturation and health and found e.g. elevated cardiovascular stress responses in migrants when they where highly adapted to the German culture.

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